Restriction map for
pFN21AE2465
(Circular) (Six-base) MAPSORT of: pFN21AE2465.seq Check: 2451 from: 1 to: 17557
pFN21AE2465 17557 bp
With 182 enzymes: SgfI *
June 14, 2013 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5320 6922 10317 11047 13828 14471 14648 5320
Size: 1602 3395 730 2781 643 177 8229
Fragments arranged by size:
8229 3395 2781 1602 730 643 177
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9192 14013 15367 278
Size: 53 83 186 936 7656 4821 1354 2468
Fragments arranged by size:
7656 4821 2468 1354 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2534 3010 5196 13496 14900 1937
Size: 597 476 2186 8300 1404 4594
Fragments arranged by size:
8300 4594 2186 1404 597 476
Acc65I G'GTAC_C
Cuts at: 14878 14878
Size: 17557
AclI AA'CG_TT
Cuts at: 1563 15684 16057 1563
Size: 14121 373 3063
Fragments arranged by size:
14121 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2717 2793 2924 3751 4121 5691 6530 8021
Size: 1085 76 131 827 370 1570 839 1491
Cuts at: 8021 13541 15620 17004 17272 1632
Size: 5520 2079 1384 268 1917
Fragments arranged by size:
5520 2079 1917 1570 1491 1384 1085 839
827 370 268 131 76
AfeI AGC'GCT
Cuts at: 8779 9340 8779
Size: 561 16996
AflII C'TTAA_G
Cuts at: 820 1017 12976 820
Size: 197 11959 5401
Fragments arranged by size:
11959 5401 197
AflIII A'CryG_T
Cuts at: 1203 2439 6575 10083 10800 16456 17433 1203
Size: 1236 4136 3508 717 5656 977 1327
Fragments arranged by size:
5656 4136 3508 1327 1236 977 717
AgeI A'CCGG_T
Cuts at: 16388 16388
Size: 17557
AhdI GACnn_n'nnGTC
Cuts at: 2879 3485 16286 2879
Size: 606 12801 4150
Fragments arranged by size:
12801 4150 606
AleI CACnn'nnGTG
Cuts at: 1166 3564 13556 14835 1166
Size: 2398 9992 1279 3888
Fragments arranged by size:
9992 3888 2398 1279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4219 5766 6378 10238 1184
Size: 3035 1547 612 3860 8503
Fragments arranged by size:
8503 3860 3035 1547 612
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8100 8132 8100
Size: 32 17525
AlwNI CAG_nnn'CTG
Cuts at: 4540 6131 6304 6463 6908 7721 8414 8531 10981
Size: 1591 173 159 445 813 693 117 2450
Cuts at: 10981 11725 11767 12664 12817 13966 13987 14191 14425
Size: 744 42 897 153 1149 21 204 234
Cuts at: 14425 15300 16872 4540
Size: 875 1572 5225
Fragments arranged by size:
5225 2450 1591 1572 1149 897 875 813
744 693 445 234 204 173 159 153
117 42 21
ApaI G_GGCC'C
Cuts at: 11777 14566 11777
Size: 2789 14768
ApaLI G'TGCA_C
Cuts at: 2798 4662 6467 6911 8073 9002 9313 11156 15614
Size: 1864 1805 444 1162 929 311 1843 4458
Cuts at: 15614 16770 2798
Size: 1156 3585
Fragments arranged by size:
4458 3585 1864 1843 1805 1162 1156 929
444 311
ApoI r'AATT_y
Cuts at: 1493 3758 3852 3864 4528 4811 5041 5411 5511
Size: 2265 94 12 664 283 230 370 100
Cuts at: 5511 5644 5962 6230 7396 8136 8507 9520 9764
Size: 133 318 268 1166 740 371 1013 244
Cuts at: 9764 9944 10161 10624 12408 13112 14866 15092 15244
Size: 180 217 463 1784 704 1754 226 152
Cuts at: 15244 17353 1493
Size: 2109 1697
Fragments arranged by size:
2265 2109 1784 1754 1697 1166 1013 740
704 664 463 371 370 318 283 268
244 230 226 217 180 152 133 100
94 12
AseI AT'TA_AT
Cuts at: 160 1032 2991 16111 160
Size: 872 1959 13120 1606
Fragments arranged by size:
13120 1959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 17557
AvaI C'yCGr_G
Cuts at: 1870 1943 6963 8796 9243 10631 11645 11972 12149
Size: 73 5020 1833 447 1388 1014 327 177
Cuts at: 12149 12390 13933 14486 14882 1870
Size: 241 1543 553 396 4545
Fragments arranged by size:
5020 4545 1833 1543 1388 1014 553 447
396 327 241 177 73
BamHI G'GATC_C
Cuts at: 7258 8108 11811 12130 14887 15254 7258
Size: 850 3703 319 2757 367 9561
Fragments arranged by size:
9561 3703 2757 850 367 319
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2749 3161 3181 5319 6374
Size: 325 217 627 962 412 20 2138 1055
Cuts at: 6374 7553 7891 9395 10355 10705 11178 11303 11382
Size: 1179 338 1504 960 350 473 125 79
Cuts at: 11382 11846 12601 13805 14878 16333 618
Size: 464 755 1204 1073 1455 1842
Fragments arranged by size:
2138 1842 1504 1455 1204 1179 1073 1055
962 960 755 627 473 464 412 350
338 325 217 125 79 20
BanII G_rGCy'C
Cuts at: 1391 2013 3510 4843 5375 7212 8699 9510 11145
Size: 622 1497 1333 532 1837 1487 811 1635
Cuts at: 11145 11259 11318 11777 11973 12183 12267 12308 12551
Size: 114 59 459 196 210 84 41 243
Cuts at: 12551 13940 14067 14207 14405 14566 14876 1391
Size: 1389 127 140 198 161 310 4072
Fragments arranged by size:
4072 1837 1635 1497 1487 1389 1333 811
622 532 459 310 243 210 198 196
161 140 127 114 84 59 41
BbeI G_GCGC'C
Cuts at: 7557 11307 11850 7557
Size: 3750 543 13264
Fragments arranged by size:
13264 3750 543
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2014 2442 2654 2799 2875 3146 5151
Size: 971 115 428 212 145 76 271 2005
Cuts at: 5151 5409 7019 7197 7485 8493 8677 9192 9673
Size: 258 1610 178 288 1008 184 515 481
Cuts at: 9673 9828 11583 11875 11925 11984 14054 14847 928
Size: 155 1755 292 50 59 2070 793 3638
Fragments arranged by size:
3638 2070 2005 1755 1610 1008 971 793
515 481 428 292 288 271 258 212
184 178 155 145 115 76 59 50
BbvCI CC'TCA_GC
Cuts at: 2079 4566 4638 8612 11446 2079
Size: 2487 72 3974 2834 8190
Fragments arranged by size:
8190 3974 2834 2487 72
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6051 6085 15748 15782 1903
Size: 34 4114 34 9663 34 3678
Fragments arranged by size:
9663 4114 3678 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10083 15449 16659 10083
Size: 5366 1210 10981
Fragments arranged by size:
10981 5366 1210
BclI T'GATC_A
Cuts at: 1548 4060 4542 14769 1548
Size: 2512 482 10227 4336
Fragments arranged by size:
10227 4336 2512 482
BfrBI ATG'CAT
Cuts at: 4522 13206 4522
Size: 8684 8873
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2249 4760 6833
Size: 107 122 71 631 605 577 2511 2073
Cuts at: 6833 11762 14390 16168 136
Size: 4929 2628 1778 1525
Fragments arranged by size:
4929 2628 2511 2073 1778 1525 631 605
577 122 107 71
BglII A'GATC_T
Cuts at: 3316 10539 11895 17552 3316
Size: 7223 1356 5657 3321
Fragments arranged by size:
7223 5657 3321 1356
BlpI GC'TnA_GC
Cuts at: 9462 9509 11624 12665 13894 14435 14974 9462
Size: 47 2115 1041 1229 541 539 12045
Fragments arranged by size:
12045 2115 1229 1041 541 539 47
Bme1580I G_kGCm'C
Cuts at: 1790 2802 4666 6471 6915 7666 8077 8804 8972
Size: 1012 1864 1805 444 751 411 727 168
Cuts at: 8972 9006 9317 10717 11160 11777 12606 12649 13395
Size: 34 311 1400 443 617 829 43 746
Cuts at: 13395 13889 14566 14682 15618 16774 1790
Size: 494 677 116 936 1156 2573
Fragments arranged by size:
2573 1864 1805 1400 1156 1012 936 829
751 746 727 677 617 494 444 443
411 311 168 116 43 34
BmgBI CAC'GTC
Cuts at: 1318 5572 9052 9073 10621 12021 13261 1318
Size: 4254 3480 21 1548 1400 1240 5614
Fragments arranged by size:
5614 4254 3480 1548 1400 1240 21
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3353 4615 4973
Size: 416 53 83 399 24 1936 1262 358
Cuts at: 4973 6061 8863 10827 10901 11299 14776 15288 16246
Size: 1088 2802 1964 74 398 3477 512 958
Cuts at: 16246 442
Size: 1753
Fragments arranged by size:
3477 2802 1964 1936 1753 1262 1088 958
512 416 399 398 358 83 74 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 17557
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5188 5394 6808 7804 10490 12304 14197 5188
Size: 206 1414 996 2686 1814 1893 8548
Fragments arranged by size:
8548 2686 1893 1814 1414 996 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2455 3174 3253 5469 5828 6778 6995 7136
Size: 1216 719 79 2216 359 950 217 141
Cuts at: 7136 7240 7443 7487 8592 9001 9879 10322 10351
Size: 104 203 44 1105 409 878 443 29
Cuts at: 10351 10932 10964 11186 11195 12815 13174 13271 13469
Size: 581 32 222 9 1620 359 97 198
Cuts at: 13469 14177 14465 14588 14828 16217 1239
Size: 708 288 123 240 1389 2579
Fragments arranged by size:
2579 2216 1620 1389 1216 1105 950 878
719 708 581 443 409 359 359 288
240 222 217 203 198 141 123 104
97 79 44 32 29 9
Bpu10I CC'TnA_GC
Cuts at: 2079 4566 4638 5151 5504 7070 8612 11446 14076
Size: 2487 72 513 353 1566 1542 2834 2630
Cuts at: 14076 2079
Size: 5560
Fragments arranged by size:
5560 2834 2630 2487 1566 1542 513 353
72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6790 7027 12525 13219 14480 15682 16547 16845 6790
Size: 237 5498 694 1261 1202 865 298 7502
Fragments arranged by size:
7502 5498 1261 1202 865 694 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2844 5699 6611 9635 9792 9878 11299
Size: 617 1345 2855 912 3024 157 86 1421
Cuts at: 11299 13445 14141 14747 16220 882
Size: 2146 696 606 1473 2219
Fragments arranged by size:
3024 2855 2219 2146 1473 1421 1345 912
696 617 606 157 86
BsaAI yAC'GTr
Cuts at: 493 1204 6262 11358 15348 493
Size: 711 5058 5096 3990 2702
Fragments arranged by size:
5096 5058 3990 2702 711
BsaBI GATnn'nnATC
Cuts at: 7416 7463 11900 17551 7416
Size: 47 4437 5651 7422
Fragments arranged by size:
7422 5651 4437 47
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6253 7554 9189 11304
Size: 53 83 186 936 4720 1301 1635 2115
Cuts at: 11304 11742 11847 14010 15364 15746 16381 275
Size: 438 105 2163 1354 382 635 1451
Fragments arranged by size:
4720 2163 2115 1635 1451 1354 1301 936
635 438 382 186 105 83 53
BsaWI w'CCGG_w
Cuts at: 4627 8897 14337 15990 16388 16662 16809 17220 4627
Size: 4270 5440 1653 398 274 147 411 4964
Fragments arranged by size:
5440 4964 4270 1653 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2453 2483 5049 5079 8585
Size: 30 165 30 1050 30 2566 30 3506
Cuts at: 8585 8615 9097 9127 9242 9272 9877 9907 10329
Size: 30 482 30 115 30 605 30 422
Cuts at: 10329 10359 11462 11492 12290 12320 14150 14180 14576
Size: 30 1103 30 798 30 1830 30 396
Cuts at: 14576 14606 1178
Size: 30 4129
Fragments arranged by size:
4129 3506 2566 1830 1103 1050 798 605
482 422 396 165 115 30 30 30
30 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2571 2636 3177 7088 9105 10322 10332
Size: 1012 373 65 541 3911 2017 1217 10
Cuts at: 10332 10526 10681 11188 11291 11470 11473 11476 11516
Size: 194 155 507 103 179 3 3 40
Cuts at: 11516 11560 12740 13808 13970 14276 14600 14779 1186
Size: 44 1180 1068 162 306 324 179 3964
Fragments arranged by size:
3964 3911 2017 1217 1180 1068 1012 541
507 373 324 306 194 179 179 162
155 103 65 44 40 10 3 3
BseYI C'CCAG_C
Cuts at: 3987 6995 7262 7270 8475 8774 8780 9087 10099
Size: 3008 267 8 1205 299 6 307 1012
Cuts at: 10099 10807 11519 11725 13889 13987 14184 16760 17256
Size: 708 712 206 2164 98 197 2576 496
Cuts at: 17256 3987
Size: 4288
Fragments arranged by size:
4288 3008 2576 2164 1205 1012 712 708
496 307 299 267 206 197 98 8
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3333 4595 4699 6405 6764 10043 10484
Size: 573 1597 1262 104 1706 359 3279 441
Cuts at: 10484 12244 13292 13613 13952 14296 1163
Size: 1760 1048 321 339 344 4424
Fragments arranged by size:
4424 3279 1760 1706 1597 1262 1048 573
441 359 344 339 321 104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3809 8995 9266 11334 11454 12779
Size: 275 186 1808 5186 271 2068 120 1325
Cuts at: 12779 14385 15007 15768 16796 1540
Size: 1606 622 761 1028 2301
Fragments arranged by size:
5186 2301 2068 1808 1606 1325 1028 761
622 275 271 186 120
BsiHKAI G_wGCw'C
Cuts at: 2174 2795 2802 3358 4666 4843 6471 6649 6915
Size: 621 7 556 1308 177 1628 178 266
Cuts at: 6915 7212 7954 8077 8638 9006 9317 9510 10308
Size: 297 742 123 561 368 311 193 798
Cuts at: 10308 10725 11145 11160 11259 11625 12908 12954 15618
Size: 417 420 15 99 366 1283 46 2664
Cuts at: 15618 15703 16774 2174
Size: 85 1071 2957
Fragments arranged by size:
2957 2664 1628 1308 1283 1071 798 742
621 561 556 420 417 368 366 311
297 266 193 178 177 123 99 85
46 15 7
BsiWI C'GTAC_G
Cuts at: 6496 6496
Size: 17557
BsmI GAATG_Cn'
Cuts at: 3729 7992 13588 15068 15161 3729
Size: 4263 5596 1480 93 6125
Fragments arranged by size:
6125 5596 4263 1480 93
BsmBI CGTCTCn'nnnn_
Cuts at: 7131 9196 11273 12275 13728 14525 7131
Size: 2065 2077 1002 1453 797 10163
Fragments arranged by size:
10163 2077 2065 1453 1002 797
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2174 2795 2802 3358 3510 4666
Size: 399 223 161 621 7 556 152 1156
Cuts at: 4666 4843 5375 6471 6649 6915 7212 7666 7954
Size: 177 532 1096 178 266 297 454 288
Cuts at: 7954 8077 8638 8699 8804 8972 9006 9317 9510
Size: 123 561 61 105 168 34 311 193
Cuts at: 9510 10308 10717 10725 11145 11160 11259 11318 11625
Size: 798 409 8 420 15 99 59 307
Cuts at: 11625 11777 11973 12183 12267 12308 12551 12606 12649
Size: 152 196 210 84 41 243 55 43
Cuts at: 12649 12908 12954 13395 13889 13940 14067 14207 14405
Size: 259 46 441 494 51 127 140 198
Cuts at: 14405 14566 14682 14876 15618 15703 16774 1391
Size: 161 116 194 742 85 1071 2174
Fragments arranged by size:
2174 1156 1096 1071 798 742 621 561
556 532 494 454 441 420 409 399
311 307 297 288 266 259 243 223
210 198 196 194 193 178 177 168
161 161 152 152 140 127 123 116
105 99 85 84 61 59 55 51
46 43 41 34 15 8 7
BspEI T'CCGG_A
Cuts at: 14337 17220 14337
Size: 2883 14674
BspHI T'CATG_A
Cuts at: 5493 14766 15446 17176 5493
Size: 9273 680 1730 5874
Fragments arranged by size:
9273 5874 1730 680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4387 5320 6586 6922 10015 10317 11047 11337
Size: 3543 933 1266 336 3093 302 730 290
Cuts at: 11337 13828 14094 14223 14471 14648 14711 14765 14912
Size: 2491 266 129 248 177 63 54 147
Cuts at: 14912 844
Size: 3489
Fragments arranged by size:
3543 3489 3093 2491 1266 933 730 336
302 290 266 248 177 147 129 63
54
BsrBI CCG'CTC
Cuts at: 1394 1823 9461 9691 9891 15444 1394
Size: 429 7638 230 200 5553 3507
Fragments arranged by size:
7638 5553 3507 429 230 200
BsrDI GCAATG_nn'
Cuts at: 65 1248 7508 8288 9304 9558 9685 10399 16052
Size: 1183 6260 780 1016 254 127 714 5653
Cuts at: 16052 16226 65
Size: 174 1396
Fragments arranged by size:
6260 5653 1396 1183 1016 780 714 254
174 127
BsrFI r'CCGG_y
Cuts at: 1700 1810 2670 11333 12143 12230 14286 14393 16201
Size: 110 860 8663 810 87 2056 107 1808
Cuts at: 16201 16388 1700
Size: 187 2869
Fragments arranged by size:
8663 2869 2056 1808 860 810 187 110
107 87
BsrGI T'GTAC_A
Cuts at: 96 5247 7959 10698 12312 96
Size: 5151 2712 2739 1614 5341
Fragments arranged by size:
5341 5151 2739 2712 1614
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 17557
BssSI C'ACGA_G
Cuts at: 5435 6079 15617 16629 5435
Size: 644 9538 1012 6363
Fragments arranged by size:
9538 6363 1012 644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3047 8072 11862 13966 14086 794
Size: 2253 5025 3790 2104 120 4265
Fragments arranged by size:
5025 4265 3790 2253 2104 120
BstBI TT'CG_AA
Cuts at: 9865 16361 9865
Size: 6496 11061
BstEII G'GTnAC_C
Cuts at: 1184 4487 11336 11440 13558 13754 1184
Size: 3303 6849 104 2118 196 4987
Fragments arranged by size:
6849 4987 3303 2118 196 104
BstXI CCAn_nnnn'nTGG
Cuts at: 3482 6995 10132 11098 11239 11824 13705 3482
Size: 3513 3137 966 141 585 1881 7334
Fragments arranged by size:
7334 3513 3137 1881 966 585 141
BstYI r'GATC_y
Cuts at: 1972 2320 2384 2573 2832 3316 7258 7417 8108
Size: 348 64 189 259 484 3942 159 691
Cuts at: 8108 10539 11811 11895 12130 12386 12813 14887 15254
Size: 2431 1272 84 235 256 427 2074 367
Cuts at: 15254 15639 15656 17108 17194 17206 17552 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
3942 2431 2074 1977 1452 1272 691 484
427 385 367 348 346 259 256 235
189 159 86 84 64 17 12
BstZ17I GTA'TAC
Cuts at: 2535 13497 2535
Size: 10962 6595
Bsu36I CC'TnA_GG
Cuts at: 8355 9603 12818 13077 8355
Size: 1248 3215 259 12835
Fragments arranged by size:
12835 3215 1248 259
BtgI C'CryG_G
Cuts at: 513 1065 2004 3696 4757 5727 6831 9366 9450
Size: 552 939 1692 1061 970 1104 2535 84
Cuts at: 9450 10428 10709 10793 11091 11601 11872 13350 13801
Size: 978 281 84 298 510 271 1478 451
Cuts at: 13801 14196 513
Size: 395 3874
Fragments arranged by size:
3874 2535 1692 1478 1104 1061 978 970
939 552 510 451 395 298 281 271
84 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3584 6043 9591 11731 13492 487
Size: 683 56 2358 2459 3548 2140 1761 4552
Fragments arranged by size:
4552 3548 2459 2358 2140 1761 683 56
BtsI GCAGTG_nn'
Cuts at: 4054 5381 5627 6739 8020 8825 9925 10051 14667
Size: 1327 246 1112 1281 805 1100 126 4616
Cuts at: 14667 15073 15867 15887 4054
Size: 406 794 20 5724
Fragments arranged by size:
5724 4616 1327 1281 1112 1100 805 794
406 246 126 20
ClaI AT'CG_AT
Cuts at: 1554 3429 13209 1554
Size: 1875 9780 5902
Fragments arranged by size:
9780 5902 1875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 8637 8672 529
Size: 35 8073 35 9414
Fragments arranged by size:
9414 8073 35 35
DraI TTT'AAA
Cuts at: 3255 3757 3903 4743 4816 14861 3255
Size: 502 146 840 73 10045 5951
Fragments arranged by size:
10045 5951 840 502 146 73
DraIII CAC_nnn'GTG
Cuts at: 4140 6975 7659 8072 10747 12190 12892 4140
Size: 2835 684 413 2675 1443 702 8805
Fragments arranged by size:
8805 2835 2675 1443 702 684 413
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 16564 809
Size: 507 15248 1802
Fragments arranged by size:
15248 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5000 5092 6306
Size: 54 1425 50 275 18 3170 92 1214
Cuts at: 6306 6825 8992 9206 9263 9453 10197 10482 10738
Size: 519 2167 214 57 190 744 285 256
Cuts at: 10738 10759 11229 11331 11394 12776 13743 14193 14382
Size: 21 470 102 63 1382 967 450 189
Cuts at: 14382 15004 15893 17522 8
Size: 622 889 1629 43
Fragments arranged by size:
3170 2167 1629 1425 1382 1214 967 889
744 622 519 470 450 285 275 256
214 190 189 102 92 63 57 54
50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 8992 9263 11331 12776 14382 15004 1537
Size: 275 7180 271 2068 1445 1606 622 4090
Fragments arranged by size:
7180 4090 2068 1606 1445 622 275 271
EarI CTCTTCn'nnn_
Cuts at: 1353 2548 3510 4704 4713 4882 6011 6708 7422
Size: 1195 962 1194 9 169 1129 697 714
Cuts at: 7422 7620 8301 9029 9324 9771 10274 10578 12489
Size: 198 681 728 295 447 503 304 1911
Cuts at: 12489 12764 15487 1353
Size: 275 2723 3423
Fragments arranged by size:
3423 2723 1911 1195 1194 1129 962 728
714 697 681 503 447 304 295 275
198 169 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4565 8703 10414 11684 12049 13811
Size: 1064 138 2976 4138 1711 1270 365 1762
Cuts at: 13811 16144 16518 16664 387
Size: 2333 374 146 1280
Fragments arranged by size:
4138 2976 2333 1762 1711 1280 1270 1064
374 365 146 138
EcoICRI GAG'CTC
Cuts at: 4841 7210 9508 11143 11257 4841
Size: 2369 2298 1635 114 11141
Fragments arranged by size:
11141 2369 2298 1635 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2455 2717 2793 2924 3174 3253 3751
Size: 393 823 262 76 131 250 79 498
Cuts at: 3751 4121 5469 5691 5828 6530 6778 6995 7136
Size: 370 1348 222 137 702 248 217 141
Cuts at: 7136 7240 7443 7487 8021 8592 9001 9879 10322
Size: 104 203 44 534 571 409 878 443
Cuts at: 10322 10351 10932 10964 11186 11195 12815 13174 13271
Size: 29 581 32 222 9 1620 359 97
Cuts at: 13271 13469 13541 14177 14465 14588 14828 15620 16217
Size: 198 72 636 288 123 240 792 597
Cuts at: 16217 17004 17272 1239
Size: 787 268 1524
Fragments arranged by size:
1620 1524 1348 878 823 792 787 702
636 597 581 571 534 498 443 409
393 370 359 288 268 262 250 248
240 222 222 217 203 198 141 137
131 123 104 97 79 76 72 44
32 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2708 3189 8394 10502 10775 11054 11828 13520 13835
Size: 481 5205 2108 273 279 774 1692 315
Cuts at: 13835 14230 2708
Size: 395 6035
Fragments arranged by size:
6035 5205 2108 1692 774 481 395 315
279 273
EcoO109I rG'GnC_Cy
Cuts at: 2328 2378 4916 6096 6554 8839 9352 9720 10095
Size: 50 2538 1180 458 2285 513 368 375
Cuts at: 10095 11713 11764 11774 13039 14161 14562 14563 14660
Size: 1618 51 10 1265 1122 401 1 97
Cuts at: 14660 2328
Size: 5225
Fragments arranged by size:
5225 2538 2285 1618 1265 1180 1122 513
458 401 375 368 97 51 50 10
1
EcoRI G'AATT_C
Cuts at: 5411 5962 7396 8507 14866 15244 5411
Size: 551 1434 1111 6359 378 7724
Fragments arranged by size:
7724 6359 1434 1111 551 378
EcoRV GAT'ATC
Cuts at: 6147 13141 14101 6147
Size: 6994 960 9603
Fragments arranged by size:
9603 6994 960
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3899 7417 9739 3899
Size: 3518 2322 11717
Fragments arranged by size:
11717 3518 2322
FseI GG_CCGG'CC
Cuts at: 14397 14397
Size: 17557
FspI TGC'GCA
Cuts at: 6667 11150 13201 14459 16063 6667
Size: 4483 2051 1258 1604 8161
Fragments arranged by size:
8161 4483 2051 1604 1258
FspAI rTGC'GCAy
Cuts at: 13201 13201
Size: 17557
HaeII r_GCGC'y
Cuts at: 1678 7172 7557 8781 9169 9342 11307 11587 11850
Size: 5494 385 1224 388 173 1965 280 263
Cuts at: 11850 12057 16704 1678
Size: 207 4647 2531
Fragments arranged by size:
5494 4647 2531 1965 1224 388 385 280
263 207 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2312 2344 2493 2525 2824
Size: 32 135 32 740 32 149 32 299
Cuts at: 2824 2856 2865 2897 2960 2992 3471 3503 5049
Size: 32 9 32 63 32 479 32 1546
Cuts at: 5049 5081 7472 7504 9097 9129 9680 9712 11462
Size: 32 2391 32 1593 32 551 32 1750
Cuts at: 11462 11494 11835 11867 12290 12322 12371 12403 13644
Size: 32 341 32 423 32 49 32 1241
Cuts at: 13644 13676 16198 16230 16272 16304 1373
Size: 32 2522 32 42 32 2626
Fragments arranged by size:
2626 2522 2391 1750 1593 1546 1241 740
551 479 423 341 299 149 135 63
49 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 10612 12489 14901 15147 669
Size: 988 281 8674 1877 2412 246 3079
Fragments arranged by size:
8674 3079 2412 1877 988 281 246
HindIII A'AGCT_T
Cuts at: 748 14774 748
Size: 14026 3531
HpaI GTT'AAC
Cuts at: 15147 15147
Size: 17557
KasI G'GCGC_C
Cuts at: 7553 11303 11846 7553
Size: 3750 543 13264
Fragments arranged by size:
13264 3750 543
KpnI G_GTAC'C
Cuts at: 14882 14882
Size: 17557
MfeI C'AATT_G
Cuts at: 3648 7970 9852 15156 3648
Size: 4322 1882 5304 6049
Fragments arranged by size:
6049 5304 4322 1882
MluI A'CGCG_T
Cuts at: 10800 17433 10800
Size: 6633 10924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2530 2607 3099 4362 5428 7373 7564 9907
Size: 1225 77 492 1263 1066 1945 191 2343
Cuts at: 9907 10068 11063 11232 12156 12276 13240 16671 16855
Size: 161 995 169 924 120 964 3431 184
Cuts at: 16855 1305
Size: 2007
Fragments arranged by size:
3431 2343 2007 1945 1263 1225 1066 995
964 924 492 191 184 169 161 120
77
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6308 6827 9208 9455 10199
Size: 54 1425 343 4476 519 2381 247 744
Cuts at: 10199 10484 10761 11231 13745 14195 10
Size: 285 277 470 2514 450 3372
Fragments arranged by size:
4476 3372 2514 2381 1425 744 519 470
450 343 285 277 247 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3564 5844 9009 9297 10619 11096 11237
Size: 648 2398 2280 3165 288 1322 477 141
Cuts at: 11237 13329 13556 14297 14747 14765 14835 15515 15874
Size: 2092 227 741 450 18 70 680 359
Cuts at: 15874 16033 518
Size: 159 2042
Fragments arranged by size:
3165 2398 2280 2092 2042 1322 741 680
648 477 450 359 288 227 159 141
70 18
MspA1I CmG'CkG
Cuts at: 1606 2655 4579 5740 6358 6491 6867 7156 8531
Size: 1049 1924 1161 618 133 376 289 1375
Cuts at: 8531 10765 10795 10841 10978 11186 11215 11908 12136
Size: 2234 30 46 137 208 29 693 228
Cuts at: 12136 13352 13604 13705 13812 13898 13963 13984 14017
Size: 1216 252 101 107 86 65 21 33
Cuts at: 14017 14422 14973 15650 16798 17043 1606
Size: 405 551 677 1148 245 2120
Fragments arranged by size:
2234 2120 1924 1375 1216 1161 1148 1049
693 677 618 551 405 376 289 252
245 228 208 137 133 107 101 86
65 46 33 30 29 21
NaeI GCC'GGC
Cuts at: 12145 14288 14395 12145
Size: 2143 107 15307
Fragments arranged by size:
15307 2143 107
NarI GG'CG_CC
Cuts at: 7554 11304 11847 7554
Size: 3750 543 13264
Fragments arranged by size:
13264 3750 543
NcoI C'CATG_G
Cuts at: 513 1065 2004 3696 4757 5727 6831 9366 13801
Size: 552 939 1692 1061 970 1104 2535 4435
Cuts at: 13801 513
Size: 4269
Fragments arranged by size:
4435 4269 2535 1692 1104 1061 970 939
552
NdeI CA'TA_TG
Cuts at: 387 13765 387
Size: 13378 4179
NgoMIV G'CCGG_C
Cuts at: 12143 14286 14393 12143
Size: 2143 107 15307
Fragments arranged by size:
15307 2143 107
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 17557
NotI GC'GGCC_GC
Cuts at: 15004 15004
Size: 17557
NruI TCG'CGA
Cuts at: 8704 8704
Size: 17557
NsiI A_TGCA'T
Cuts at: 4524 13208 4524
Size: 8684 8873
NspI r_CATG'y
Cuts at: 1132 1229 2443 3403 3414 5669 5821 6053 6187
Size: 97 1214 960 11 2255 152 232 134
Cuts at: 6187 6475 6579 7838 8463 8734 9205 10087 11656
Size: 288 104 1259 625 271 471 882 1569
Cuts at: 11656 13206 13223 14764 14915 16460 1132
Size: 1550 17 1541 151 1545 2229
Fragments arranged by size:
2255 2229 1569 1550 1545 1541 1259 1214
960 882 625 471 288 271 232 152
151 134 104 97 17 11
PasI CC'CwG_GG
Cuts at: 1348 3446 12551 1348
Size: 2098 9105 6354
Fragments arranged by size:
9105 6354 2098
PciI A'CATG_T
Cuts at: 2439 6575 10083 16456 2439
Size: 4136 3508 6373 3540
Fragments arranged by size:
6373 4136 3540 3508
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6837 7659 10910 11134 11767 14191 1108
Size: 5729 822 3251 224 633 2424 4474
Fragments arranged by size:
5729 4474 3251 2424 822 633 224
PfoI T'CCnGG_A
Cuts at: 5485 6600 7011 7324 7420 9118 10287 10909 10985
Size: 1115 411 313 96 1698 1169 622 76
Cuts at: 10985 11807 5485
Size: 822 11235
Fragments arranged by size:
11235 1698 1169 1115 822 622 411 313
96 76
PmeI GTTT'AAAC
Cuts at: 14861 14861
Size: 17557
PmlI CAC'GTG
Cuts at: 6262 6262
Size: 17557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 11279 11311 15626 15658 17139 17171 11279
Size: 32 4315 32 1481 32 11665
Fragments arranged by size:
11665 4315 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2328 6096 6554 11713 13039 14660 2328
Size: 3768 458 5159 1326 1621 5225
Fragments arranged by size:
5225 5159 3768 1621 1326 458
PshAI GACnn'nnGTC
Cuts at: 1613 6539 11045 1613
Size: 4926 4506 8125
Fragments arranged by size:
8125 4926 4506
PsiI TTA'TAA
Cuts at: 3542 9498 15127 3542
Size: 5956 5629 5972
Fragments arranged by size:
5972 5956 5629
PspOMI G'GGCC_C
Cuts at: 11773 14562 11773
Size: 2789 14768
PspXI vC'TCGA_Gb
Cuts at: 12149 14486 12149
Size: 2337 15220
PstI C_TGCA'G
Cuts at: 830 5634 5745 6300 7636 7858 10557 11058 11913
Size: 4804 111 555 1336 222 2699 501 855
Cuts at: 11913 12089 13431 13857 14091 14234 14364 14421 14909
Size: 176 1342 426 234 143 130 57 488
Cuts at: 14909 830
Size: 3478
Fragments arranged by size:
4804 3478 2699 1342 1336 855 555 501
488 426 234 222 176 143 130 111
57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 17557
PvuII CAG'CTG
Cuts at: 4579 5740 6358 6491 7156 8531 10765 10978 11215
Size: 1161 618 133 665 1375 2234 213 237
Cuts at: 11215 13705 13898 13963 13984 14422 4579
Size: 2490 193 65 21 438 7714
Fragments arranged by size:
7714 2490 2234 1375 1161 665 618 438
237 213 193 133 65 21
SacI G_AGCT'C
Cuts at: 4843 7212 9510 11145 11259 4843
Size: 2369 2298 1635 114 11141
Fragments arranged by size:
11141 2369 2298 1635 114
SacII CC_GC'GG
Cuts at: 10796 13353 10796
Size: 2557 15000
SalI G'TCGA_C
Cuts at: 1936 14899 1936
Size: 12963 4594
SanDI GG'GwC_CC
Cuts at: 2328 6096 14660 2328
Size: 3768 8564 5225
Fragments arranged by size:
8564 5225 3768
SapI GCTCTTCn'nnn_
Cuts at: 4713 4882 9029 4713
Size: 169 4147 13241
Fragments arranged by size:
13241 4147 169
SbfI CC_TGCA'GG
Cuts at: 11058 14091 14909 11058
Size: 3033 818 13706
Fragments arranged by size:
13706 3033 818
ScaI AGT'ACT
Cuts at: 14786 15805 17517 14786
Size: 1019 1712 14826
Fragments arranged by size:
14826 1712 1019
SfcI C'TryA_G
Cuts at: 826 1045 2337 4391 5630 5741 6296 6337 7632
Size: 219 1292 2054 1239 111 555 41 1295
Cuts at: 7632 7698 7854 8190 8215 8925 10553 11054 11909
Size: 66 156 336 25 710 1628 501 855
Cuts at: 11909 12085 12513 13427 13853 14087 14230 14360 14417
Size: 176 428 914 426 234 143 130 57
Cuts at: 14417 14905 16040 16721 16912 826
Size: 488 1135 681 191 1471
Fragments arranged by size:
2054 1628 1471 1295 1292 1239 1135 914
855 710 681 555 501 488 428 426
336 234 219 191 176 156 143 130
111 66 57 41 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 6833 14390 6833
Size: 7557 10000
SfoI GGC'GCC
Cuts at: 7555 11305 11848 7555
Size: 3750 543 13264
Fragments arranged by size:
13264 3750 543
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 17557
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 17557
SmaI CCC'GGG
Cuts at: 1872 8798 10633 14884 1872
Size: 6926 1835 4251 4545
Fragments arranged by size:
6926 4545 4251 1835
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6769 7042 12149 12540 12976 13234
Size: 197 926 4826 273 5107 391 436 258
Cuts at: 13234 14486 14495 15661 16562 16824 820
Size: 1252 9 1166 901 262 1553
Fragments arranged by size:
5107 4826 1553 1252 1166 926 901 436
391 273 262 258 197 9
SnaBI TAC'GTA
Cuts at: 493 11358 15348 493
Size: 10865 3990 2702
Fragments arranged by size:
10865 3990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 17557
SphI G_CATG'C
Cuts at: 1132 8463 13206 14915 1132
Size: 7331 4743 1709 3774
Fragments arranged by size:
7331 4743 3774 1709
SspI AAT'ATT
Cuts at: 5 52 3286 15481 5
Size: 47 3234 12195 2081
Fragments arranged by size:
12195 3234 2081 47
StuI AGG'CCT
Cuts at: 1517 7674 8790 11567 11861 12077 13232 1517
Size: 6157 1116 2777 294 216 1155 5842
Fragments arranged by size:
6157 5842 2777 1155 1116 294 216
StyI C'CwwG_G
Cuts at: 513 1065 2004 2127 3696 4757 5727 6831 7596
Size: 552 939 123 1569 1061 970 1104 765
Cuts at: 7596 9132 9366 9645 12936 13629 13801 14067 14996
Size: 1536 234 279 3291 693 172 266 929
Cuts at: 14996 513
Size: 3074
Fragments arranged by size:
3291 3074 1569 1536 1104 1061 970 939
929 765 693 552 279 266 234 172
123
SwaI ATTT'AAAT
Cuts at: 3757 3757
Size: 17557
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2629 3160 4367 6003 6797 8221 8682
Size: 1255 1049 531 1207 1636 794 1424 461
Cuts at: 8682 11834 12824 13140 13504 14165 14594 15584 15601
Size: 3152 990 316 364 661 429 990 17
Cuts at: 15601 15754 15939 16384 325
Size: 153 185 445 1498
Fragments arranged by size:
3152 1636 1498 1424 1255 1207 1049 990
990 794 661 531 461 445 429 364
316 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2607 5247 7959
Size: 274 80 33 51 1443 630 2640 2712
Cuts at: 7959 8326 9144 10206 10698 12312 12861 13275 14784
Size: 367 818 1062 492 1614 549 414 1509
Cuts at: 14784 15803 17515 96
Size: 1019 1712 138
Fragments arranged by size:
2712 2640 1712 1614 1509 1443 1062 1019
818 630 549 492 414 367 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4761 4793 6746 6778 8371 8403 8585 8617 10329
Size: 32 1953 32 1593 32 182 32 1712
Cuts at: 10329 10361 11642 11674 11973 12005 12176 12208 14150
Size: 32 1281 32 299 32 171 32 1942
Cuts at: 14150 14182 14576 14608 17517 17549 4761
Size: 32 394 32 2909 32 4769
Fragments arranged by size:
4769 2909 1953 1942 1712 1593 1281 394
299 182 171 32 32 32 32 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2505 6784 10877 1316
Size: 1189 4279 4093 7996
Fragments arranged by size:
7996 4279 4093 1189
XbaI T'CTAG_A
Cuts at: 7946 8261 14893 7946
Size: 315 6632 10610
Fragments arranged by size:
10610 6632 315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4361 6608 10129 10612 14515 4361
Size: 2247 3521 483 3903 7403
Fragments arranged by size:
7403 3903 3521 2247 483
XhoI C'TCGA_G
Cuts at: 1943 12149 14486 1943
Size: 10206 2337 5014
Fragments arranged by size:
10206 5014 2337
XmaI C'CCGG_G
Cuts at: 1870 8796 10631 14882 1870
Size: 6926 1835 4251 4545
Fragments arranged by size:
6926 4545 4251 1835
XmnI GAAnn'nnTTC
Cuts at: 2282 5559 7992 8224 12498 13104 15686 2282
Size: 3277 2433 232 4274 606 2582 4153
Fragments arranged by size:
4274 4153 3277 2582 2433 606 232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9190 14011 15365 276
Size: 53 83 186 936 7656 4821 1354 2468
Fragments arranged by size:
7656 4821 2468 1354 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI PacI PsrI RsrII SexAI SrfI