Restriction map for
pFN21AE2465
(Linear) (Six-base) MAPSORT of: KIEE2465.seq Check: 7778 from: 1 to: 12852
KIEE2465.orf
With 182 enzymes: SgfI *
June 14, 2013 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3315 4917 8312 9042 11823 12466 12643 12852
Size: 3315 1602 3395 730 2781 643 177 209
Fragments arranged by size:
3395 3315 2781 1602 730 643 209 177
AatII G_ACGT'C
Cuts at: 0 7187 12008 12852
Size: 7187 4821 844
Fragments arranged by size:
7187 4821 844
AccI GT'mk_AC
Cuts at: 0 529 1005 3191 11491 12852
Size: 529 476 2186 8300 1361
Fragments arranged by size:
8300 2186 1361 529 476
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 788 919 1746 2116 3686 4525 6016
Size: 712 76 131 827 370 1570 839 1491
Cuts at: 6016 11536 12852
Size: 5520 1316
Fragments arranged by size:
5520 1570 1491 1316 839 827 712 370
131 76
AfeI AGC'GCT
Cuts at: 0 6774 7335 12852
Size: 6774 561 5517
Fragments arranged by size:
6774 5517 561
AflII C'TTAA_G
Cuts at: 0 10971 12852
Size: 10971 1881
AflIII A'CryG_T
Cuts at: 0 434 4570 8078 8795 12852
Size: 434 4136 3508 717 4057
Fragments arranged by size:
4136 4057 3508 717 434
AhdI GACnn_n'nnGTC
Cuts at: 0 874 1480 12852
Size: 874 606 11372
Fragments arranged by size:
11372 874 606
AleI CACnn'nnGTG
Cuts at: 0 1559 11551 12830 12852
Size: 1559 9992 1279 22
Fragments arranged by size:
9992 1559 1279 22
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2214 3761 4373 8233 12852
Size: 2214 1547 612 3860 4619
Fragments arranged by size:
4619 3860 2214 1547 612
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 6095 6127 12852
Size: 6095 32 6725
Fragments arranged by size:
6725 6095 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2535 4126 4299 4458 4903 5716 6409 6526
Size: 2535 1591 173 159 445 813 693 117
Cuts at: 6526 8976 9720 9762 10659 10812 11961 11982 12186
Size: 2450 744 42 897 153 1149 21 204
Cuts at: 12186 12420 12852
Size: 234 432
Fragments arranged by size:
2535 2450 1591 1149 897 813 744 693
445 432 234 204 173 159 153 117
42 21
ApaI G_GGCC'C
Cuts at: 0 9772 12561 12852
Size: 9772 2789 291
Fragments arranged by size:
9772 2789 291
ApaLI G'TGCA_C
Cuts at: 0 793 2657 4462 4906 6068 6997 7308 9151
Size: 793 1864 1805 444 1162 929 311 1843
Cuts at: 9151 12852
Size: 3701
Fragments arranged by size:
3701 1864 1843 1805 1162 929 793 444
311
ApoI r'AATT_y
Cuts at: 0 1753 1847 1859 2523 2806 3036 3406 3506
Size: 1753 94 12 664 283 230 370 100
Cuts at: 3506 3639 3957 4225 5391 6131 6502 7515 7759
Size: 133 318 268 1166 740 371 1013 244
Cuts at: 7759 7939 8156 8619 10403 11107 12852
Size: 180 217 463 1784 704 1745
Fragments arranged by size:
1784 1753 1745 1166 1013 740 704 664
463 371 370 318 283 268 244 230
217 180 133 100 94 12
AseI AT'TA_AT
Cuts at: 0 986 12852
Size: 986 11866
AvaI C'yCGr_G
Cuts at: 0 4958 6791 7238 8626 9640 9967 10144 10385
Size: 4958 1833 447 1388 1014 327 177 241
Cuts at: 10385 11928 12481 12852
Size: 1543 553 371
Fragments arranged by size:
4958 1833 1543 1388 1014 553 447 371
327 241 177
BamHI G'GATC_C
Cuts at: 0 5253 6103 9806 10125 12852
Size: 5253 850 3703 319 2727
Fragments arranged by size:
5253 3703 2727 850 319
BanI G'GyrC_C
Cuts at: 0 744 1156 1176 3314 4369 5548 5886 7390
Size: 744 412 20 2138 1055 1179 338 1504
Cuts at: 7390 8350 8700 9173 9298 9377 9841 10596 11800
Size: 960 350 473 125 79 464 755 1204
Cuts at: 11800 12852
Size: 1052
Fragments arranged by size:
2138 1504 1204 1179 1055 1052 960 755
744 473 464 412 350 338 125 79
20
BanII G_rGCy'C
Cuts at: 0 8 1505 2838 3370 5207 6694 7505 9140
Size: 8 1497 1333 532 1837 1487 811 1635
Cuts at: 9140 9254 9313 9772 9968 10178 10262 10303 10546
Size: 114 59 459 196 210 84 41 243
Cuts at: 10546 11935 12062 12202 12400 12561 12852
Size: 1389 127 140 198 161 291
Fragments arranged by size:
1837 1635 1497 1487 1389 1333 811 532
459 291 243 210 198 196 161 140
127 114 84 59 41 8
BbeI G_GCGC'C
Cuts at: 0 5552 9302 9845 12852
Size: 5552 3750 543 3007
Fragments arranged by size:
5552 3750 3007 543
BbsI GAAGACnn'nnnn_
Cuts at: 0 9 437 649 794 870 1141 3146 3404
Size: 9 428 212 145 76 271 2005 258
Cuts at: 3404 5014 5192 5480 6488 6672 7187 7668 7823
Size: 1610 178 288 1008 184 515 481 155
Cuts at: 7823 9578 9870 9920 9979 12049 12842 12852
Size: 1755 292 50 59 2070 793 10
Fragments arranged by size:
2070 2005 1755 1610 1008 793 515 481
428 292 288 271 258 212 184 178
155 145 76 59 50 10 9
BbvCI CC'TCA_GC
Cuts at: 0 74 2561 2633 6607 9441 12852
Size: 74 2487 72 3974 2834 3411
Fragments arranged by size:
3974 3411 2834 2487 74 72
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4046 4080 12852
Size: 4046 34 8772
Fragments arranged by size:
8772 4046 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 8078 12852
Size: 8078 4774
BclI T'GATC_A
Cuts at: 0 2055 2537 12764 12852
Size: 2055 482 10227 88
Fragments arranged by size:
10227 2055 482 88
BfrBI ATG'CAT
Cuts at: 0 2517 11201 12852
Size: 2517 8684 1651
Fragments arranged by size:
8684 2517 1651
BglI GCCn_nnn'nGGC
Cuts at: 0 244 2755 4828 9757 12385 12852
Size: 244 2511 2073 4929 2628 467
Fragments arranged by size:
4929 2628 2511 2073 467 244
BglII A'GATC_T
Cuts at: 0 1311 8534 9890 12852
Size: 1311 7223 1356 2962
Fragments arranged by size:
7223 2962 1356 1311
BlpI GC'TnA_GC
Cuts at: 0 7457 7504 9619 10660 11889 12430 12852
Size: 7457 47 2115 1041 1229 541 422
Fragments arranged by size:
7457 2115 1229 1041 541 422 47
Bme1580I G_kGCm'C
Cuts at: 0 797 2661 4466 4910 5661 6072 6799 6967
Size: 797 1864 1805 444 751 411 727 168
Cuts at: 6967 7001 7312 8712 9155 9772 10601 10644 11390
Size: 34 311 1400 443 617 829 43 746
Cuts at: 11390 11884 12561 12677 12852
Size: 494 677 116 175
Fragments arranged by size:
1864 1805 1400 829 797 751 746 727
677 617 494 444 443 411 311 175
168 116 43 34
BmgBI CAC'GTC
Cuts at: 0 3567 7047 7068 8616 10016 11256 12852
Size: 3567 3480 21 1548 1400 1240 1596
Fragments arranged by size:
3567 3480 1596 1548 1400 1240 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 1348 2610 2968 4056 6858 8822 8896 9294
Size: 1348 1262 358 1088 2802 1964 74 398
Cuts at: 9294 12771 12852
Size: 3477 81
Fragments arranged by size:
3477 2802 1964 1348 1262 1088 398 358
81 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3183 3389 4803 5799 8485 10299 12192 12852
Size: 3183 206 1414 996 2686 1814 1893 660
Fragments arranged by size:
3183 2686 1893 1814 1414 996 660 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 1169 1248 3464 3823 4773 4990 5131
Size: 450 719 79 2216 359 950 217 141
Cuts at: 5131 5235 5438 5482 6587 6996 7874 8317 8346
Size: 104 203 44 1105 409 878 443 29
Cuts at: 8346 8927 8959 9181 9190 10810 11169 11266 11464
Size: 581 32 222 9 1620 359 97 198
Cuts at: 11464 12172 12460 12583 12823 12852
Size: 708 288 123 240 29
Fragments arranged by size:
2216 1620 1105 950 878 719 708 581
450 443 409 359 359 288 240 222
217 203 198 141 123 104 97 79
44 32 29 29 9
Bpu10I CC'TnA_GC
Cuts at: 0 74 2561 2633 3146 3499 5065 6607 9441
Size: 74 2487 72 513 353 1566 1542 2834
Cuts at: 9441 12071 12852
Size: 2630 781
Fragments arranged by size:
2834 2630 2487 1566 1542 781 513 353
74 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4785 5022 10520 11214 12475 12852
Size: 4785 237 5498 694 1261 377
Fragments arranged by size:
5498 4785 1261 694 377 237
BsaI GGTCTCn'nnnn_
Cuts at: 0 839 3694 4606 7630 7787 7873 9294 11440
Size: 839 2855 912 3024 157 86 1421 2146
Cuts at: 11440 12136 12742 12852
Size: 696 606 110
Fragments arranged by size:
3024 2855 2146 1421 912 839 696 606
157 110 86
BsaAI yAC'GTr
Cuts at: 0 4257 9353 12852
Size: 4257 5096 3499
Fragments arranged by size:
5096 4257 3499
BsaBI GATnn'nnATC
Cuts at: 0 5411 5458 9895 12852
Size: 5411 47 4437 2957
Fragments arranged by size:
5411 4437 2957 47
BsaHI Gr'CG_yC
Cuts at: 0 4248 5549 7184 9299 9737 9842 12005 12852
Size: 4248 1301 1635 2115 438 105 2163 847
Fragments arranged by size:
4248 2163 2115 1635 1301 847 438 105
BsaWI w'CCGG_w
Cuts at: 0 2622 6892 12332 12852
Size: 2622 4270 5440 520
Fragments arranged by size:
5440 4270 2622 520
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 448 478 3044 3074 6580 6610 7092 7122
Size: 448 30 2566 30 3506 30 482 30
Cuts at: 7122 7237 7267 7872 7902 8324 8354 9457 9487
Size: 115 30 605 30 422 30 1103 30
Cuts at: 9487 10285 10315 12145 12175 12571 12601 12852
Size: 798 30 1830 30 396 30 251
Fragments arranged by size:
3506 2566 1830 1103 798 605 482 448
422 396 251 115 30 30 30 30
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 566 631 1172 5083 7100 8317 8327
Size: 193 373 65 541 3911 2017 1217 10
Cuts at: 8327 8521 8676 9183 9286 9465 9468 9471 9511
Size: 194 155 507 103 179 3 3 40
Cuts at: 9511 9555 10735 11803 11965 12271 12595 12774 12852
Size: 44 1180 1068 162 306 324 179 78
Fragments arranged by size:
3911 2017 1217 1180 1068 541 507 373
324 306 194 193 179 179 162 155
103 78 65 44 40 10 3 3
BseYI C'CCAG_C
Cuts at: 0 1982 4990 5257 5265 6470 6769 6775 7082
Size: 1982 3008 267 8 1205 299 6 307
Cuts at: 7082 8094 8802 9514 9720 11884 11982 12179 12852
Size: 1012 708 712 206 2164 98 197 673
Fragments arranged by size:
3008 2164 1982 1205 1012 712 708 673
307 299 267 206 197 98 8 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1328 2590 2694 4400 4759 8038 8479 10239
Size: 1328 1262 104 1706 359 3279 441 1760
Cuts at: 10239 11287 11608 11947 12291 12852
Size: 1048 321 339 344 561
Fragments arranged by size:
3279 1760 1706 1328 1262 1048 561 441
359 344 339 321 104
BsiEI CG_ry'CG
Cuts at: 0 1804 6990 7261 9329 9449 10774 12380 12852
Size: 1804 5186 271 2068 120 1325 1606 472
Fragments arranged by size:
5186 2068 1804 1606 1325 472 271 120
BsiHKAI G_wGCw'C
Cuts at: 0 169 790 797 1353 2661 2838 4466 4644
Size: 169 621 7 556 1308 177 1628 178
Cuts at: 4644 4910 5207 5949 6072 6633 7001 7312 7505
Size: 266 297 742 123 561 368 311 193
Cuts at: 7505 8303 8720 9140 9155 9254 9620 10903 10949
Size: 798 417 420 15 99 366 1283 46
Cuts at: 10949 12852
Size: 1903
Fragments arranged by size:
1903 1628 1308 1283 798 742 621 561
556 420 417 368 366 311 297 266
193 178 177 169 123 99 46 15
7
BsiWI C'GTAC_G
Cuts at: 0 4491 12852
Size: 4491 8361
BsmI GAATG_Cn'
Cuts at: 0 1724 5987 11583 12852
Size: 1724 4263 5596 1269
Fragments arranged by size:
5596 4263 1724 1269
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5126 7191 9268 10270 11723 12520 12852
Size: 5126 2065 2077 1002 1453 797 332
Fragments arranged by size:
5126 2077 2065 1453 1002 797 332
Bsp1286I G_dGCh'C
Cuts at: 0 8 169 790 797 1353 1505 2661 2838
Size: 8 161 621 7 556 152 1156 177
Cuts at: 2838 3370 4466 4644 4910 5207 5661 5949 6072
Size: 532 1096 178 266 297 454 288 123
Cuts at: 6072 6633 6694 6799 6967 7001 7312 7505 8303
Size: 561 61 105 168 34 311 193 798
Cuts at: 8303 8712 8720 9140 9155 9254 9313 9620 9772
Size: 409 8 420 15 99 59 307 152
Cuts at: 9772 9968 10178 10262 10303 10546 10601 10644 10903
Size: 196 210 84 41 243 55 43 259
Cuts at: 10903 10949 11390 11884 11935 12062 12202 12400 12561
Size: 46 441 494 51 127 140 198 161
Cuts at: 12561 12677 12852
Size: 116 175
Fragments arranged by size:
1156 1096 798 621 561 556 532 494
454 441 420 409 311 307 297 288
266 259 243 210 198 196 193 178
177 175 168 161 161 152 152 140
127 123 116 105 99 84 61 59
55 51 46 43 41 34 15 8
8 7
BspEI T'CCGG_A
Cuts at: 0 12332 12852
Size: 12332 520
BspHI T'CATG_A
Cuts at: 0 3488 12761 12852
Size: 3488 9273 91
Fragments arranged by size:
9273 3488 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2382 3315 4581 4917 8010 8312 9042 9332
Size: 2382 933 1266 336 3093 302 730 290
Cuts at: 9332 11823 12089 12218 12466 12643 12706 12760 12852
Size: 2491 266 129 248 177 63 54 92
Fragments arranged by size:
3093 2491 2382 1266 933 730 336 302
290 266 248 177 129 92 63 54
BsrBI CCG'CTC
Cuts at: 0 7456 7686 7886 12852
Size: 7456 230 200 4966
Fragments arranged by size:
7456 4966 230 200
BsrDI GCAATG_nn'
Cuts at: 0 5503 6283 7299 7553 7680 8394 12852
Size: 5503 780 1016 254 127 714 4458
Fragments arranged by size:
5503 4458 1016 780 714 254 127
BsrFI r'CCGG_y
Cuts at: 0 665 9328 10138 10225 12281 12388 12852
Size: 665 8663 810 87 2056 107 464
Fragments arranged by size:
8663 2056 810 665 464 107 87
BsrGI T'GTAC_A
Cuts at: 0 3242 5954 8693 10307 12852
Size: 3242 2712 2739 1614 2545
Fragments arranged by size:
3242 2739 2712 2545 1614
BssSI C'ACGA_G
Cuts at: 0 3430 4074 12852
Size: 3430 644 8778
Fragments arranged by size:
8778 3430 644
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1042 6067 9857 11961 12081 12852
Size: 1042 5025 3790 2104 120 771
Fragments arranged by size:
5025 3790 2104 1042 771 120
BstBI TT'CG_AA
Cuts at: 0 7860 12852
Size: 7860 4992
BstEII G'GTnAC_C
Cuts at: 0 2482 9331 9435 11553 11749 12852
Size: 2482 6849 104 2118 196 1103
Fragments arranged by size:
6849 2482 2118 1103 196 104
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1477 4990 8127 9093 9234 9819 11700 12852
Size: 1477 3513 3137 966 141 585 1881 1152
Fragments arranged by size:
3513 3137 1881 1477 1152 966 585 141
BstYI r'GATC_y
Cuts at: 0 315 379 568 827 1311 5253 5412 6103
Size: 315 64 189 259 484 3942 159 691
Cuts at: 6103 8534 9806 9890 10125 10381 10808 12852
Size: 2431 1272 84 235 256 427 2044
Fragments arranged by size:
3942 2431 2044 1272 691 484 427 315
259 256 235 189 159 84 64
BstZ17I GTA'TAC
Cuts at: 0 530 11492 12852
Size: 530 10962 1360
Fragments arranged by size:
10962 1360 530
Bsu36I CC'TnA_GG
Cuts at: 0 6350 7598 10813 11072 12852
Size: 6350 1248 3215 259 1780
Fragments arranged by size:
6350 3215 1780 1248 259
BtgI C'CryG_G
Cuts at: 0 1691 2752 3722 4826 7361 7445 8423 8704
Size: 1691 1061 970 1104 2535 84 978 281
Cuts at: 8704 8788 9086 9596 9867 11345 11796 12191 12852
Size: 84 298 510 271 1478 451 395 661
Fragments arranged by size:
2535 1691 1478 1104 1061 978 970 661
510 451 395 298 281 271 84 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1579 4038 7586 9726 11487 12852
Size: 1579 2459 3548 2140 1761 1365
Fragments arranged by size:
3548 2459 2140 1761 1579 1365
BtsI GCAGTG_nn'
Cuts at: 0 2049 3376 3622 4734 6015 6820 7920 8046
Size: 2049 1327 246 1112 1281 805 1100 126
Cuts at: 8046 12662 12852
Size: 4616 190
Fragments arranged by size:
4616 2049 1327 1281 1112 1100 805 246
190 126
ClaI AT'CG_AT
Cuts at: 0 1424 11204 12852
Size: 1424 9780 1648
Fragments arranged by size:
9780 1648 1424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6632 6667 12852
Size: 6632 35 6185
Fragments arranged by size:
6632 6185 35
DraI TTT'AAA
Cuts at: 0 1250 1752 1898 2738 2811 12852
Size: 1250 502 146 840 73 10041
Fragments arranged by size:
10041 1250 840 502 146 73
DraIII CAC_nnn'GTG
Cuts at: 0 2135 4970 5654 6067 8742 10185 10887 12852
Size: 2135 2835 684 413 2675 1443 702 1965
Fragments arranged by size:
2835 2675 2135 1965 1443 702 684 413
EaeI y'GGCC_r
Cuts at: 0 2995 3087 4301 4820 6987 7201 7258 7448
Size: 2995 92 1214 519 2167 214 57 190
Cuts at: 7448 8192 8477 8733 8754 9224 9326 9389 10771
Size: 744 285 256 21 470 102 63 1382
Cuts at: 10771 11738 12188 12377 12852
Size: 967 450 189 475
Fragments arranged by size:
2995 2167 1382 1214 967 744 519 475
470 450 285 256 214 190 189 102
92 63 57 21
EagI C'GGCC_G
Cuts at: 0 6987 7258 9326 10771 12377 12852
Size: 6987 271 2068 1445 1606 475
Fragments arranged by size:
6987 2068 1606 1445 475 271
EarI CTCTTCn'nnn_
Cuts at: 0 543 1505 2699 2708 2877 4006 4703 5417
Size: 543 962 1194 9 169 1129 697 714
Cuts at: 5417 5615 6296 7024 7319 7766 8269 8573 10484
Size: 198 681 728 295 447 503 304 1911
Cuts at: 10484 10759 12852
Size: 275 2093
Fragments arranged by size:
2093 1911 1194 1129 962 728 714 697
681 543 503 447 304 295 275 198
169 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2560 6698 8409 9679 10044 11806 12852
Size: 2560 4138 1711 1270 365 1762 1046
Fragments arranged by size:
4138 2560 1762 1711 1270 1046 365
EcoICRI GAG'CTC
Cuts at: 0 2836 5205 7503 9138 9252 12852
Size: 2836 2369 2298 1635 114 3600
Fragments arranged by size:
3600 2836 2369 2298 1635 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 712 788 919 1169 1248 1746 2116
Size: 450 262 76 131 250 79 498 370
Cuts at: 2116 3464 3686 3823 4525 4773 4990 5131 5235
Size: 1348 222 137 702 248 217 141 104
Cuts at: 5235 5438 5482 6016 6587 6996 7874 8317 8346
Size: 203 44 534 571 409 878 443 29
Cuts at: 8346 8927 8959 9181 9190 10810 11169 11266 11464
Size: 581 32 222 9 1620 359 97 198
Cuts at: 11464 11536 12172 12460 12583 12823 12852
Size: 72 636 288 123 240 29
Fragments arranged by size:
1620 1348 878 702 636 581 571 534
498 450 443 409 370 359 288 262
250 248 240 222 222 217 203 198
141 137 131 123 104 97 79 76
72 44 32 29 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 703 1184 6389 8497 8770 9049 9823 11515
Size: 703 481 5205 2108 273 279 774 1692
Cuts at: 11515 11830 12225 12852
Size: 315 395 627
Fragments arranged by size:
5205 2108 1692 774 703 627 481 395
315 279 273
EcoO109I rG'GnC_Cy
Cuts at: 0 323 373 2911 4091 4549 6834 7347 7715
Size: 323 50 2538 1180 458 2285 513 368
Cuts at: 7715 8090 9708 9759 9769 11034 12156 12557 12558
Size: 375 1618 51 10 1265 1122 401 1
Cuts at: 12558 12655 12852
Size: 97 197
Fragments arranged by size:
2538 2285 1618 1265 1180 1122 513 458
401 375 368 323 197 97 51 50
10 1
EcoRI G'AATT_C
Cuts at: 0 3406 3957 5391 6502 12852
Size: 3406 551 1434 1111 6350
Fragments arranged by size:
6350 3406 1434 1111 551
EcoRV GAT'ATC
Cuts at: 0 4142 11136 12096 12852
Size: 4142 6994 960 756
Fragments arranged by size:
6994 4142 960 756
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1894 5412 7734 12852
Size: 1894 3518 2322 5118
Fragments arranged by size:
5118 3518 2322 1894
FseI GG_CCGG'CC
Cuts at: 0 12392 12852
Size: 12392 460
FspI TGC'GCA
Cuts at: 0 4662 9145 11196 12454 12852
Size: 4662 4483 2051 1258 398
Fragments arranged by size:
4662 4483 2051 1258 398
FspAI rTGC'GCAy
Cuts at: 0 11196 12852
Size: 11196 1656
HaeII r_GCGC'y
Cuts at: 0 5167 5552 6776 7164 7337 9302 9582 9845
Size: 5167 385 1224 388 173 1965 280 263
Cuts at: 9845 10052 12852
Size: 207 2800
Fragments arranged by size:
5167 2800 1965 1224 388 385 280 263
207 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 488 520 819 851 860 892
Size: 307 32 149 32 299 32 9 32
Cuts at: 892 955 987 1466 1498 3044 3076 5467 5499
Size: 63 32 479 32 1546 32 2391 32
Cuts at: 5499 7092 7124 7675 7707 9457 9489 9830 9862
Size: 1593 32 551 32 1750 32 341 32
Cuts at: 9862 10285 10317 10366 10398 11639 11671 12852
Size: 423 32 49 32 1241 32 1181
Fragments arranged by size:
2391 1750 1593 1546 1241 1181 551 479
423 341 307 299 149 63 49 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 0 8607 10484 12852
Size: 8607 1877 2368
Fragments arranged by size:
8607 2368 1877
HindIII A'AGCT_T
Cuts at: 0 12769 12852
Size: 12769 83
KasI G'GCGC_C
Cuts at: 0 5548 9298 9841 12852
Size: 5548 3750 543 3011
Fragments arranged by size:
5548 3750 3011 543
MfeI C'AATT_G
Cuts at: 0 1643 5965 7847 12852
Size: 1643 4322 1882 5005
Fragments arranged by size:
5005 4322 1882 1643
MluI A'CGCG_T
Cuts at: 0 8795 12852
Size: 8795 4057
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 602 1094 2357 3423 5368 5559 7902
Size: 525 77 492 1263 1066 1945 191 2343
Cuts at: 7902 8063 9058 9227 10151 10271 11235 12852
Size: 161 995 169 924 120 964 1617
Fragments arranged by size:
2343 1945 1617 1263 1066 995 964 924
525 492 191 169 161 120 77
MscI TGG'CCA
Cuts at: 0 4303 4822 7203 7450 8194 8479 8756 9226
Size: 4303 519 2381 247 744 285 277 470
Cuts at: 9226 11740 12190 12852
Size: 2514 450 662
Fragments arranged by size:
4303 2514 2381 744 662 519 470 450
285 277 247
MslI CAynn'nnrTG
Cuts at: 0 1559 3839 7004 7292 8614 9091 9232 11324
Size: 1559 2280 3165 288 1322 477 141 2092
Cuts at: 11324 11551 12292 12742 12760 12830 12852
Size: 227 741 450 18 70 22
Fragments arranged by size:
3165 2280 2092 1559 1322 741 477 450
288 227 141 70 22 18
MspA1I CmG'CkG
Cuts at: 0 650 2574 3735 4353 4486 4862 5151 6526
Size: 650 1924 1161 618 133 376 289 1375
Cuts at: 6526 8760 8790 8836 8973 9181 9210 9903 10131
Size: 2234 30 46 137 208 29 693 228
Cuts at: 10131 11347 11599 11700 11807 11893 11958 11979 12012
Size: 1216 252 101 107 86 65 21 33
Cuts at: 12012 12417 12852
Size: 405 435
Fragments arranged by size:
2234 1924 1375 1216 1161 693 650 618
435 405 376 289 252 228 208 137
133 107 101 86 65 46 33 30
29 21
NaeI GCC'GGC
Cuts at: 0 10140 12283 12390 12852
Size: 10140 2143 107 462
Fragments arranged by size:
10140 2143 462 107
NarI GG'CG_CC
Cuts at: 0 5549 9299 9842 12852
Size: 5549 3750 543 3010
Fragments arranged by size:
5549 3750 3010 543
NcoI C'CATG_G
Cuts at: 0 1691 2752 3722 4826 7361 11796 12852
Size: 1691 1061 970 1104 2535 4435 1056
Fragments arranged by size:
4435 2535 1691 1104 1061 1056 970
NdeI CA'TA_TG
Cuts at: 0 11760 12852
Size: 11760 1092
NgoMIV G'CCGG_C
Cuts at: 0 10138 12281 12388 12852
Size: 10138 2143 107 464
Fragments arranged by size:
10138 2143 464 107
NruI TCG'CGA
Cuts at: 0 6699 12852
Size: 6699 6153
NsiI A_TGCA'T
Cuts at: 0 2519 11203 12852
Size: 2519 8684 1649
Fragments arranged by size:
8684 2519 1649
NspI r_CATG'y
Cuts at: 0 438 1398 1409 3664 3816 4048 4182 4470
Size: 438 960 11 2255 152 232 134 288
Cuts at: 4470 4574 5833 6458 6729 7200 8082 9651 11201
Size: 104 1259 625 271 471 882 1569 1550
Cuts at: 11201 11218 12759 12852
Size: 17 1541 93
Fragments arranged by size:
2255 1569 1550 1541 1259 960 882 625
471 438 288 271 232 152 134 104
93 17 11
PasI CC'CwG_GG
Cuts at: 0 1441 10546 12852
Size: 1441 9105 2306
Fragments arranged by size:
9105 2306 1441
PciI A'CATG_T
Cuts at: 0 434 4570 8078 12852
Size: 434 4136 3508 4774
Fragments arranged by size:
4774 4136 3508 434
PflMI CCAn_nnn'nTGG
Cuts at: 0 4832 5654 8905 9129 9762 12186 12852
Size: 4832 822 3251 224 633 2424 666
Fragments arranged by size:
4832 3251 2424 822 666 633 224
PfoI T'CCnGG_A
Cuts at: 0 3480 4595 5006 5319 5415 7113 8282 8904
Size: 3480 1115 411 313 96 1698 1169 622
Cuts at: 8904 8980 9802 12852
Size: 76 822 3050
Fragments arranged by size:
3480 3050 1698 1169 1115 822 622 411
313 96 76
PmlI CAC'GTG
Cuts at: 0 4257 12852
Size: 4257 8595
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 9274 9306 12852
Size: 9274 32 3546
Fragments arranged by size:
9274 3546 32
PpuMI rG'GwC_Cy
Cuts at: 0 323 4091 4549 9708 11034 12655 12852
Size: 323 3768 458 5159 1326 1621 197
Fragments arranged by size:
5159 3768 1621 1326 458 323 197
PshAI GACnn'nnGTC
Cuts at: 0 4534 9040 12852
Size: 4534 4506 3812
Fragments arranged by size:
4534 4506 3812
PsiI TTA'TAA
Cuts at: 0 1537 7493 12852
Size: 1537 5956 5359
Fragments arranged by size:
5956 5359 1537
PspOMI G'GGCC_C
Cuts at: 0 9768 12557 12852
Size: 9768 2789 295
Fragments arranged by size:
9768 2789 295
PspXI vC'TCGA_Gb
Cuts at: 0 10144 12481 12852
Size: 10144 2337 371
Fragments arranged by size:
10144 2337 371
PstI C_TGCA'G
Cuts at: 0 3629 3740 4295 5631 5853 8552 9053 9908
Size: 3629 111 555 1336 222 2699 501 855
Cuts at: 9908 10084 11426 11852 12086 12229 12359 12416 12852
Size: 176 1342 426 234 143 130 57 436
Fragments arranged by size:
3629 2699 1342 1336 855 555 501 436
426 234 222 176 143 130 111 57
PvuII CAG'CTG
Cuts at: 0 2574 3735 4353 4486 5151 6526 8760 8973
Size: 2574 1161 618 133 665 1375 2234 213
Cuts at: 8973 9210 11700 11893 11958 11979 12417 12852
Size: 237 2490 193 65 21 438 435
Fragments arranged by size:
2574 2490 2234 1375 1161 665 618 438
435 237 213 193 133 65 21
SacI G_AGCT'C
Cuts at: 0 2838 5207 7505 9140 9254 12852
Size: 2838 2369 2298 1635 114 3598
Fragments arranged by size:
3598 2838 2369 2298 1635 114
SacII CC_GC'GG
Cuts at: 0 8791 11348 12852
Size: 8791 2557 1504
Fragments arranged by size:
8791 2557 1504
SanDI GG'GwC_CC
Cuts at: 0 323 4091 12655 12852
Size: 323 3768 8564 197
Fragments arranged by size:
8564 3768 323 197
SapI GCTCTTCn'nnn_
Cuts at: 0 2708 2877 7024 12852
Size: 2708 169 4147 5828
Fragments arranged by size:
5828 4147 2708 169
SbfI CC_TGCA'GG
Cuts at: 0 9053 12086 12852
Size: 9053 3033 766
Fragments arranged by size:
9053 3033 766
ScaI AGT'ACT
Cuts at: 0 12781 12852
Size: 12781 71
SfcI C'TryA_G
Cuts at: 0 332 2386 3625 3736 4291 4332 5627 5693
Size: 332 2054 1239 111 555 41 1295 66
Cuts at: 5693 5849 6185 6210 6920 8548 9049 9904 10080
Size: 156 336 25 710 1628 501 855 176
Cuts at: 10080 10508 11422 11848 12082 12225 12355 12412 12852
Size: 428 914 426 234 143 130 57 440
Fragments arranged by size:
2054 1628 1295 1239 914 855 710 555
501 440 428 426 336 332 234 176
156 143 130 111 66 57 41 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4828 12385 12852
Size: 4828 7557 467
Fragments arranged by size:
7557 4828 467
SfoI GGC'GCC
Cuts at: 0 5550 9300 9843 12852
Size: 5550 3750 543 3009
Fragments arranged by size:
5550 3750 3009 543
SmaI CCC'GGG
Cuts at: 0 6793 8628 12852
Size: 6793 1835 4224
Fragments arranged by size:
6793 4224 1835
SmlI C'TyrA_G
Cuts at: 0 4764 5037 10144 10535 10971 11229 12481 12490
Size: 4764 273 5107 391 436 258 1252 9
Cuts at: 12490 12852
Size: 362
Fragments arranged by size:
5107 4764 1252 436 391 362 273 258
9
SnaBI TAC'GTA
Cuts at: 0 9353 12852
Size: 9353 3499
SphI G_CATG'C
Cuts at: 0 6458 11201 12852
Size: 6458 4743 1651
Fragments arranged by size:
6458 4743 1651
SspI AAT'ATT
Cuts at: 0 1281 12852
Size: 1281 11571
StuI AGG'CCT
Cuts at: 0 5669 6785 9562 9856 10072 11227 12852
Size: 5669 1116 2777 294 216 1155 1625
Fragments arranged by size:
5669 2777 1625 1155 1116 294 216
StyI C'CwwG_G
Cuts at: 0 122 1691 2752 3722 4826 5591 7127 7361
Size: 122 1569 1061 970 1104 765 1536 234
Cuts at: 7361 7640 10931 11624 11796 12062 12852
Size: 279 3291 693 172 266 790
Fragments arranged by size:
3291 1569 1536 1104 1061 970 790 765
693 279 266 234 172 122
SwaI ATTT'AAAT
Cuts at: 0 1752 12852
Size: 1752 11100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 624 1155 2362 3998 4792 6216 6677 9829
Size: 624 531 1207 1636 794 1424 461 3152
Cuts at: 9829 10819 11135 11499 12160 12589 12852
Size: 990 316 364 661 429 263
Fragments arranged by size:
3152 1636 1424 1207 990 794 661 624
531 461 429 364 316 263
TatI w'GTAC_w
Cuts at: 0 602 3242 5954 6321 7139 8201 8693 10307
Size: 602 2640 2712 367 818 1062 492 1614
Cuts at: 10307 10856 11270 12779 12852
Size: 549 414 1509 73
Fragments arranged by size:
2712 2640 1614 1509 1062 818 602 549
492 414 367 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2756 2788 4741 4773 6366 6398 6580 6612
Size: 2756 32 1953 32 1593 32 182 32
Cuts at: 6612 8324 8356 9637 9669 9968 10000 10171 10203
Size: 1712 32 1281 32 299 32 171 32
Cuts at: 10203 12145 12177 12571 12603 12852
Size: 1942 32 394 32 249
Fragments arranged by size:
2756 1953 1942 1712 1593 1281 394 299
249 182 171 32 32 32 32 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 500 4779 8872 12852
Size: 500 4279 4093 3980
Fragments arranged by size:
4279 4093 3980 500
XbaI T'CTAG_A
Cuts at: 0 5941 6256 12852
Size: 5941 315 6596
Fragments arranged by size:
6596 5941 315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2356 4603 8124 8607 12510 12852
Size: 2356 2247 3521 483 3903 342
Fragments arranged by size:
3903 3521 2356 2247 483 342
XhoI C'TCGA_G
Cuts at: 0 10144 12481 12852
Size: 10144 2337 371
Fragments arranged by size:
10144 2337 371
XmaI C'CCGG_G
Cuts at: 0 6791 8626 12852
Size: 6791 1835 4226
Fragments arranged by size:
6791 4226 1835
XmnI GAAnn'nnTTC
Cuts at: 0 277 3554 5987 6219 10493 11099 12852
Size: 277 3277 2433 232 4274 606 1753
Fragments arranged by size:
4274 3277 2433 1753 606 277 232
ZraI GAC'GTC
Cuts at: 0 7185 12006 12852
Size: 7185 4821 846
Fragments arranged by size:
7185 4821 846
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII KasI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NruI NsiI NspI PasI PciI PflMI PfoI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuII
SacI SacII SanDI SapI SbfI ScaI SfcI SfiI
SfoI SmaI SmlI SnaBI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AgeI AscI AsiSI AvrII BaeI BmtI
BssHII DrdI HpaI KpnI NheI NotI PacI PmeI
PsrI PvuI RsrII SalI SexAI SgfI SgrAI SpeI
SrfI