################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsgI BsmBI BsmI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstPI BstXI BstYI Bsu36I ClaI CpoI DraI DraIII Eam1105I EarI Eco47III Eco81I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I MamI MfeI MflI MluNI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 603 TCCGGA T^CCGGA AclI 305 AACGTT AA^CGTT Alw44I 508 GTGCAC G^TGCAC AlwI 265 GATCC GGATC(4/5) ApaLI 508 GTGCAC G^TGCAC Asp700I 132 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 220 CTCGGG C^[CT]CG[GA]G AvaII 9 GGTCC G^G[AT]CC BanI 200 GGCACC G^G[CT][GA]CC BcgI 620 GCACGTCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 197 CCCGG CC^[GC]GG BlpI 769 GCTGAGC GC^T[ACGT]AGC BpmI 246 CTGGAG CTGGAG(16/14) Bpu1102I 769 GCTGAGC GC^T[ACGT]AGC BsaWI 603 TCCGGA [AT]^CCGG[AT] BseAI 603 TCCGGA T^CCGGA BseRI 151 CTCCTC GAGGAG(10/8) BsiEI 113 CGGCCG CG[GA][CT]^CG BsiWI 17 CGTACG C^GTACG BsoBI 220 CTCGGG C^[CT]CG[GA]G BspEI 603 TCCGGA T^CCGGA BspHI 780 TCATGA T^CATGA BsrDI 765 CATTGC GCAATG(2/0) BsrI 105 ACTGG ACTGG(1/-1) BstF5I 606 GGATG GGATG(2/0) CelII 769 GCTGAGC GC^T[ACGT]AGC DraII 146 AGGGCCT [GA]G^G[ACGT]CC[CT] DrdI 871 GACATCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 209 CCGCGG C^C[GA][CT]GG EaeI 113 CGGCCG [CT]^GGCC[GA] EagI 113 CGGCCG C^GGCCG EclXI 113 CGGCCG C^GGCCG Eco52I 113 CGGCCG C^GGCCG EcoNI 153 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 146 AGGGCCT [GA]G^G[ACGT]CC[CT] FauI 35 CCCGC CCCGC(4/6) FokI 606 GGATG GGATG(9/13) HgaI 181 GACGC GACGC(5/10) KspI 209 CCGCGG CCGC^GG MboII 702 TCTTC GAAGA(8/7) MluI 182 ACGCGT A^CGCGT MroI 603 TCCGGA T^CCGGA MslI 420 CATTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 209 CCGCGG C[AC]G^C[GT]G NciI 197 CCCGG CC^[GC]GG NlaIV 200 GGCACC GG[ACGT]^[ACGT]CC Psp1406I 305 AACGTT AA^CGTT RcaI 780 TCATGA T^CATGA SacII 209 CCGCGG CCGC^GG SfaNI 607 GATGC GCATC(5/9) TaqI 629 TCGA T^CGA TthHB8I 629 TCGA T^CGA XmnI 132 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 743 GTATAC GT^[AC][GT]AC 903 GTATAC AcsI 350 GAATTC [GA]^AATT[CT] 726 GAATTC AfaI 18 GTAC GT^AC 331 GTAC AflIII 182 ACGCGT A^C[GA][CT]GT 298 ACGTGT ApoI 350 GAATTC [GA]^AATT[CT] 726 GAATTC BfaI 335 CTAG C^TAG 640 CTAG BsmAI 251 GAGAC GTCTC(1/5) 644 GAGAC BsrFI 76 GCCGGC [GA]^CCGG[CT] 115 GCCGGC Bst1107I 743 GTATAC GTA^TAC 903 GTATAC BstAPI 410 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 820 GCACAGTTTGC BstZ17I 743 GTATAC GTA^TAC 903 GTATAC CfoI 38 GCGC GCG^C 164 GCGC Cfr10I 76 GCCGGC [GA]^CCGG[CT] 115 GCCGGC DpnII 265 GATC ^GATC 515 GATC EcoRI 350 GAATTC G^AATTC 726 GAATTC HhaI 38 GCGC GCG^C 164 GCGC HinP1I 38 GCGC G^CGC 164 GCGC MaeI 335 CTAG C^TAG 640 CTAG MboI 265 GATC ^GATC 515 GATC MseI 289 TTAA T^TAA 721 TTAA NaeI 76 GCCGGC GCC^GGC 115 GCCGGC NdeII 265 GATC ^GATC 515 GATC NgoMIV 76 GCCGGC G^CCGGC 115 GCCGGC NspI 683 ACATGC [GA]CATG^[CT] 829 GCATGT PleI 671 GACTC GAGTC(4/5) 584 GAGTC RsaI 18 GTAC GT^AC 331 GTAC Sau3AI 265 GATC ^GATC 515 GATC SmlI 154 CTCAAG C^T[CT][GA]AG 591 CTTGAG TfiI 472 GATTC G^A[AT]TC 699 GATTC Tru9I 289 TTAA T^TAA 721 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AccII AciI AluI AspHI BbvI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstUI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MnlI MspI MvaI MvnI MwoI NlaIII Sau96I ScrFI TseI Tsp45I Tsp509I