Restriction map for
pFN21AE5361
(Circular) (Six-base) MAPSORT of: pFN21AE5361.seq Check: 7460 from: 1 to: 5617
pFN21AE5361 5617 bp
With 182 enzymes: SgfI *
January 30, 2014 17:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3427 278
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2749 2909 2960 1937
Size: 812 160 51 4594
Fragments arranged by size:
4594 812 160 51
Acc65I G'GTAC_C
Cuts at: 2938 2938
Size: 5617
AclI AA'CG_TT
Cuts at: 1563 2311 3744 4117 1563
Size: 748 1433 373 3063
Fragments arranged by size:
3063 1433 748 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2598 2693 3680 5064 5332 1632
Size: 966 95 987 1384 268 1917
Fragments arranged by size:
1917 1384 987 966 268 95
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5420
AflIII A'CryG_T
Cuts at: 1203 2187 2303 4516 5493 1203
Size: 984 116 2213 977 1327
Fragments arranged by size:
2213 1327 984 977 116
AgeI A'CCGG_T
Cuts at: 4448 4448
Size: 5617
AhdI GACnn_n'nnGTC
Cuts at: 4346 4346
Size: 5617
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2447 1184
Size: 1263 4354
AlwNI CAG_nnn'CTG
Cuts at: 3360 4932 3360
Size: 1572 4045
ApaLI G'TGCA_C
Cuts at: 2513 3674 4830 2513
Size: 1161 1156 3300
Fragments arranged by size:
3300 1161 1156
ApoI r'AATT_y
Cuts at: 1493 2355 2731 2926 3152 3304 5413 1493
Size: 862 376 195 226 152 2109 1697
Fragments arranged by size:
2109 1697 862 376 226 195 152
AseI AT'TA_AT
Cuts at: 160 1032 4171 160
Size: 872 3139 1606
Fragments arranged by size:
3139 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
AvaI C'yCGr_G
Cuts at: 1870 1943 2225 2942 1870
Size: 73 282 717 4545
Fragments arranged by size:
4545 717 282 73
BamHI G'GATC_C
Cuts at: 2947 3314 2947
Size: 367 5250
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2205 2938 4393 618
Size: 325 217 627 418 733 1455 1842
Fragments arranged by size:
1842 1455 733 627 418 325 217
BanII G_rGCy'C
Cuts at: 1391 2936 1391
Size: 1545 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4646
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2614 2648 3808 3842 1903
Size: 34 677 34 1160 34 3678
Fragments arranged by size:
3678 1160 677 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3509 4719 3509
Size: 1210 4407
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5617
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4228 136
Size: 107 122 71 631 605 2556 1525
Fragments arranged by size:
2556 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5612 5612
Size: 5617
BlpI GC'TnA_GC
Cuts at: 2775 3034 2775
Size: 259 5358
Bme1580I G_kGCm'C
Cuts at: 1790 2517 3678 4834 1790
Size: 727 1161 1156 2573
Fragments arranged by size:
2573 1161 1156 727
BmgBI CAC'GTC
Cuts at: 1318 2221 2304 2628 1318
Size: 903 83 324 4307
Fragments arranged by size:
4307 903 324 83
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3348 4306 442
Size: 416 53 83 399 24 1931 958 1753
Fragments arranged by size:
1931 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2272 4277 1239
Size: 1033 2005 2579
Fragments arranged by size:
2579 2005 1033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 2617 3742 4607 4905 2144
Size: 473 1125 865 298 2856
Fragments arranged by size:
2856 1125 865 473 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4280 882
Size: 617 2781 2219
Fragments arranged by size:
2781 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3408 493
Size: 711 2204 2702
Fragments arranged by size:
2702 2204 711
BsaBI GATnn'nnATC
Cuts at: 5611 5611
Size: 5617
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3424 3806 4441 275
Size: 53 83 186 936 1891 382 635 1451
Fragments arranged by size:
1891 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2608 4050 4448 4722 4869 5280 2608
Size: 1442 398 274 147 411 2945
Fragments arranged by size:
2945 1442 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5392
Fragments arranged by size:
5392 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2147 1186
Size: 961 4656
BseYI C'CCAG_C
Cuts at: 4820 5316 4820
Size: 496 5121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5044
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2121 3067 3828 4856 1540
Size: 275 186 120 946 761 1028 2301
Fragments arranged by size:
2301 1028 946 761 275 186 120
BsiHKAI G_wGCw'C
Cuts at: 2052 2226 2517 3678 3763 4834 2052
Size: 174 291 1161 85 1071 2835
Fragments arranged by size:
2835 1161 1071 291 174 85
BsiWI C'GTAC_G
Cuts at: 2022 2022
Size: 5617
BsmI GAATG_Cn'
Cuts at: 3128 3221 3128
Size: 93 5524
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2226 2517 2936 3678 3763 4834
Size: 399 262 174 291 419 742 85 1071
Cuts at: 4834 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 419 399 291 262 174
85
BspEI T'CCGG_A
Cuts at: 2608 5280 2608
Size: 2672 2945
BspHI T'CATG_A
Cuts at: 2785 3506 5236 2785
Size: 721 1730 3166
Fragments arranged by size:
3166 1730 721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2972 844
Size: 2128 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3504 1394
Size: 429 1681 3507
Fragments arranged by size:
3507 1681 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2769 4112 4286 65
Size: 1183 1521 1343 174 1396
Fragments arranged by size:
1521 1396 1343 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2081 2120 4261 4448 1700
Size: 110 271 39 2141 187 2869
Fragments arranged by size:
2869 2141 271 187 110 39
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5617
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5617
BssSI C'ACGA_G
Cuts at: 3677 4689 3677
Size: 1012 4605
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2421 2831 794
Size: 1627 410 3580
Fragments arranged by size:
3580 1627 410
BstBI TT'CG_AA
Cuts at: 4421 4421
Size: 5617
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5617
BstYI r'GATC_y
Cuts at: 1972 2947 3314 3699 3716 5168 5254 5266 5612
Size: 975 367 385 17 1452 86 12 346
Cuts at: 5612 1972
Size: 1977
Fragments arranged by size:
1977 1452 975 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2750 2910 2750
Size: 160 5457
BtgI C'CryG_G
Cuts at: 513 1065 2214 513
Size: 552 1149 3916
Fragments arranged by size:
3916 1149 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4878
Fragments arranged by size:
4878 683 56
BtsI GCAGTG_nn'
Cuts at: 3133 3927 3947 3133
Size: 794 20 4803
Fragments arranged by size:
4803 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5582
DraI TTT'AAA
Cuts at: 2921 2921
Size: 5617
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2882 4624 809
Size: 507 1566 1742 1802
Fragments arranged by size:
1802 1742 1566 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 3064 3953
Size: 54 1425 50 275 18 288 946 889
Cuts at: 3953 5582 8
Size: 1629 43
Fragments arranged by size:
1629 1425 946 889 288 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2118 3064 1537
Size: 275 306 946 4090
Fragments arranged by size:
4090 946 306 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3547 1353
Size: 2194 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4204 4578 4724 387
Size: 1064 138 2615 374 146 1280
Fragments arranged by size:
2615 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2272 2598 2693 3680 4277 5064 5332
Size: 393 640 326 95 987 597 787 268
Cuts at: 5332 1239
Size: 1524
Fragments arranged by size:
1524 987 787 640 597 393 326 268
95
EcoNI CCTnn'n_nnAGG
Cuts at: 2162 2162
Size: 5617
EcoO109I rG'GnC_Cy
Cuts at: 2152 2152
Size: 5617
EcoRI G'AATT_C
Cuts at: 2355 2731 2926 3304 2355
Size: 376 195 378 4668
Fragments arranged by size:
4668 378 376 195
FspI TGC'GCA
Cuts at: 4123 4123
Size: 5617
HaeII r_GCGC'y
Cuts at: 1678 4764 1678
Size: 3086 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2590 2612 2622 2640 2644
Size: 32 135 32 1018 22 10 18 4
Cuts at: 2644 2672 4258 4290 4332 4364 1373
Size: 28 1586 32 42 32 2626
Fragments arranged by size:
2626 1586 1018 135 42 32 32 32
32 28 22 18 10 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2961 3207 669
Size: 988 281 1023 246 3079
Fragments arranged by size:
3079 1023 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5617
HpaI GTT'AAC
Cuts at: 3207 3207
Size: 5617
KpnI G_GTAC'C
Cuts at: 2942 2942
Size: 5617
MfeI C'AATT_G
Cuts at: 3216 3216
Size: 5617
MluI A'CGCG_T
Cuts at: 2187 5493 2187
Size: 3306 2311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4731 4915 1305
Size: 3426 184 2007
Fragments arranged by size:
3426 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3795
Fragments arranged by size:
3795 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2429 3575 3934 4093 518
Size: 648 1263 1146 359 159 2042
Fragments arranged by size:
2042 1263 1146 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2216 3033 3710 4858 5103 1606
Size: 610 817 677 1148 245 2120
Fragments arranged by size:
2120 1148 817 677 610 245
NaeI GCC'GGC
Cuts at: 2083 2122 2083
Size: 39 5578
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5065
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5617
NgoMIV G'CCGG_C
Cuts at: 2081 2120 2081
Size: 39 5578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5617
NotI GC'GGCC_GC
Cuts at: 3064 3064
Size: 5617
NspI r_CATG'y
Cuts at: 1132 1229 2692 2838 2975 4520 1132
Size: 97 1463 146 137 1545 2229
Fragments arranged by size:
2229 1545 1463 146 137 97
PasI CC'CwG_GG
Cuts at: 1348 2076 1348
Size: 728 4889
PciI A'CATG_T
Cuts at: 4516 4516
Size: 5617
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5617
PmeI GTTT'AAAC
Cuts at: 2921 2921
Size: 5617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3718 5199 5231 3686
Size: 32 1481 32 4072
Fragments arranged by size:
4072 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5617
PsiI TTA'TAA
Cuts at: 3187 3187
Size: 5617
PstI C_TGCA'G
Cuts at: 830 2969 830
Size: 2139 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5617
SacII CC_GC'GG
Cuts at: 2217 2217
Size: 5617
SalI G'TCGA_C
Cuts at: 1936 2959 1936
Size: 1023 4594
SbfI CC_TGCA'GG
Cuts at: 2969 2969
Size: 5617
ScaI AGT'ACT
Cuts at: 3865 5577 3865
Size: 1712 3905
SfcI C'TryA_G
Cuts at: 826 1045 2965 4100 4781 4972 826
Size: 219 1920 1135 681 191 1471
Fragments arranged by size:
1920 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5617
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5617
SmaI CCC'GGG
Cuts at: 1872 2944 1872
Size: 1072 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 2596 3721 4622 4884 820
Size: 197 926 216 437 1125 901 262 1553
Fragments arranged by size:
1553 1125 926 901 437 262 216 197
SnaBI TAC'GTA
Cuts at: 493 3408 493
Size: 2915 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5617
SphI G_CATG'C
Cuts at: 1132 2975 1132
Size: 1843 3774
SspI AAT'ATT
Cuts at: 5 52 3541 5
Size: 47 3489 2081
Fragments arranged by size:
3489 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5617
StyI C'CwwG_G
Cuts at: 513 1065 3056 513
Size: 552 1991 3074
Fragments arranged by size:
3074 1991 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2002 2084 3644 3661 3814 3999 4444
Size: 1255 422 82 1560 17 153 185 445
Cuts at: 4444 325
Size: 1498
Fragments arranged by size:
1560 1498 1255 445 422 185 153 82
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3863 5575 96
Size: 274 80 33 51 1443 1886 1712 138
Fragments arranged by size:
1886 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2174 2206 2838 2870 5577 5609 2174
Size: 32 632 32 2707 32 2182
Fragments arranged by size:
2707 2182 632 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5617
XbaI T'CTAG_A
Cuts at: 2953 2953
Size: 5617
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5617
XmaI C'CCGG_G
Cuts at: 1870 2942 1870
Size: 1072 4545
XmnI GAAnn'nnTTC
Cuts at: 2141 3746 2141
Size: 1605 4012
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3425 276
Size: 53 83 186 936 1891 2468
Fragments arranged by size:
2468 1891 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsmBI BstXI Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI