################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI BsmI BspHI BspLU11I BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HindIII HinfI HpaI HphI KasI KpnI MaeII MamI MfeI MluI MluNI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 39 CGCG CG^CG AccIII 200 TCCGGA T^CCGGA Aor51HI 489 AGCGCT AGC^GCT ApaI 61 GGGCCC GGGCC^C Asp700I 435 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 70 CTCGAG C^[CT]CG[GA]G BanII 61 GGGCCC G[GA]GC[CT]^C BbsI 316 GTCTTC GAAGAC(2/6) BcnI 59 CCGGG CC^[GC]GG BfaI 550 CTAG C^TAG BglII 437 AGATCT A^GATCT BlpI 607 GCTGAGC GC^T[ACGT]AGC BmyI 61 GGGCCC G[AGT]GC[ACT]^C Bpu1102I 607 GCTGAGC GC^T[ACGT]AGC BpuAI 316 GTCTTC GAAGAC(2/6) BsaI 55 GAGACC GGTCTC(1/5) BsaWI 200 TCCGGA [AT]^CCGG[AT] BseAI 200 TCCGGA T^CCGGA BsgI 430 GTGCAG GTGCAG(16/14) BsmAI 55 GAGAC GTCTC(1/5) BsoBI 70 CTCGAG C^[CT]CG[GA]G Bsp1286I 61 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 590 TGTACA T^GTACA BspDI 571 ATCGAT AT^CGAT BspEI 200 TCCGGA T^CCGGA BspMI 86 ACCTGC ACCTGC(4/8) BsrBI 343 GAGCGG CCGCTC(-3/-3) BsrGI 590 TGTACA T^GTACA BstUI 39 CGCG CG^CG CelII 607 GCTGAGC GC^T[ACGT]AGC ClaI 571 ATCGAT AT^CGAT DdeI 608 CTGAG C^T[ACGT]AG EaeI 11 TGGCCG [CT]^GGCC[GA] EarI 544 CTCTTC CTCTTC(1/4) Eco47III 489 AGCGCT AGC^GCT Ksp632I 544 CTCTTC CTCTTC(1/4) KspI 38 CCGCGG CCGC^GG MaeI 550 CTAG C^TAG MaeIII 511 GTTAC ^GT[ACGT]AC MroI 200 TCCGGA T^CCGGA MseI 614 TTAA T^TAA MslI 594 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 39 CGCG CG^CG NciI 59 CCGGG CC^[GC]GG NcoI 417 CCATGG C^CATGG NlaIII 418 CATG CATG^ PaeR7I 70 CTCGAG C^TCGAG PpuMI 112 GGGTCCC [GA]G^G[AT]CC[CT] PstI 208 CTGCAG CTGCA^G SacII 38 CCGCGG CCGC^GG SfaNI 329 GCATC GCATC(5/9) SfcI 208 CTGCAG C^T[GA][CT]AG SspBI 590 TGTACA T^GTACA Tru9I 614 TTAA T^TAA XhoI 70 CTCGAG C^TCGAG XmnI 435 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AluI 171 AGCT AG^CT 611 AGCT AlwI 537 GATCC GGATC(4/5) 425 GGATC AvaII 113 GGTCC G^G[AT]CC 203 GGACC BglI 268 GCCACACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 382 GCCACCAAGGC BseRI 517 GAGGAG GAGGAG(10/8) 520 GAGGAG BsrI 273 ACTGG ACTGG(1/-1) 118 CCAGT BstYI 437 AGATCT [GA]^GATC[CT] 536 AGATCC Cac8I 9 GCTGGC GC[ACGT]^[ACGT]GC 36 GCCCGC DraII 61 GGGCCCT [GA]G^G[ACGT]CC[CT] 112 GGGTCCC DsaI 38 CCGCGG C^C[GA][CT]GG 417 CCATGG EcoO109I 61 GGGCCCT [GA]G^G[ACGT]CC[CT] 112 GGGTCCC FauI 37 CCCGC CCCGC(4/6) 345 GCGGG HapII 59 CCGG C^CGG 201 CCGG HincII 376 GTCAAC GT[CT]^[GA]AC 553 GTCAAC HindII 376 GTCAAC GT[CT]^[GA]AC 553 GTCAAC HpaII 59 CCGG C^CGG 201 CCGG MflI 437 AGATCT [GA]^GATC[CT] 536 AGATCC MspA1I 31 CCGCTG C[AC]G^C[GT]G 38 CCGCGG MspI 59 CCGG C^CGG 201 CCGG SmlI 70 CTCGAG C^T[CT][GA]AG 406 CTCAAG StuI 297 AGGCCT AGG^CCT 389 AGGCCT XhoII 437 AGATCT [GA]^GATC[CT] 536 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AfaI BbvI BpmI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI CfoI Cfr13I DpnII EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HhaI HinP1I ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI StyI TaqI TseI TspRI TthHB8I