(Circular) (Six-base) MAPSORT of: pFN21AE6256.seq Check: 7638 from: 1 to: 5332 pFN21AE6256 5332 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2100 2100 Size: 5332 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3142 278 Size: 53 83 186 936 1606 2468 Fragments arranged by size: 2468 1606 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2675 1937 Size: 738 4594 Acc65I G'GTAC_C Cuts at: 2653 2653 Size: 5332 AclI AA'CG_TT Cuts at: 1563 3459 3832 1563 Size: 1896 373 3063 Fragments arranged by size: 3063 1896 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2051 3395 4779 5047 1632 Size: 419 1344 1384 268 1917 Fragments arranged by size: 1917 1384 1344 419 268 AfeI AGC'GCT Cuts at: 2496 2496 Size: 5332 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5135 AflIII A'CryG_T Cuts at: 1203 4231 5208 1203 Size: 3028 977 1327 Fragments arranged by size: 3028 1327 977 AgeI A'CCGG_T Cuts at: 4163 4163 Size: 5332 AhdI GACnn_n'nnGTC Cuts at: 4061 4061 Size: 5332 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5332 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5332 AlwNI CAG_nnn'CTG Cuts at: 3075 4647 3075 Size: 1572 3760 ApaI G_GGCC'C Cuts at: 2070 2070 Size: 5332 ApaLI G'TGCA_C Cuts at: 3389 4545 3389 Size: 1156 4176 ApoI r'AATT_y Cuts at: 1493 2641 2867 3019 5128 1493 Size: 1148 226 152 2109 1697 Fragments arranged by size: 2109 1697 1148 226 152 AseI AT'TA_AT Cuts at: 160 1032 3886 160 Size: 872 2854 1606 Fragments arranged by size: 2854 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5332 AvaI C'yCGr_G Cuts at: 1870 1943 2075 2657 1870 Size: 73 132 582 4545 Fragments arranged by size: 4545 582 132 73 BamHI G'GATC_C Cuts at: 2662 3029 2662 Size: 367 4965 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2653 4108 618 Size: 325 217 627 866 1455 1842 Fragments arranged by size: 1842 1455 866 627 325 217 BanII G_rGCy'C Cuts at: 1391 2070 2651 1391 Size: 679 581 4072 Fragments arranged by size: 4072 679 581 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2314 928 Size: 971 415 3946 Fragments arranged by size: 3946 971 415 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3523 3557 1903 Size: 34 1586 34 3678 Fragments arranged by size: 3678 1586 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3224 4434 3224 Size: 1210 4122 BclI T'GATC_A Cuts at: 1548 1548 Size: 5332 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2279 2393 3943 Size: 107 122 71 631 605 607 114 1550 Cuts at: 3943 136 Size: 1525 Fragments arranged by size: 1550 1525 631 607 605 122 114 107 71 BglII A'GATC_T Cuts at: 2442 5327 2442 Size: 2885 2447 BlpI GC'TnA_GC Cuts at: 2613 2749 2613 Size: 136 5196 Bme1580I G_kGCm'C Cuts at: 1790 2070 3393 4549 1790 Size: 280 1323 1156 2573 Fragments arranged by size: 2573 1323 1156 280 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5332 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2117 3063 4021 Size: 416 53 83 399 24 700 946 958 Cuts at: 4021 442 Size: 1753 Fragments arranged by size: 1753 958 946 700 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5332 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2246 2312 2555 3992 1239 Size: 1007 66 243 1437 2579 Fragments arranged by size: 2579 1437 1007 243 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2396 3457 4322 4620 2396 Size: 1061 865 298 3108 Fragments arranged by size: 3108 1061 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2054 3995 882 Size: 617 555 1941 2219 Fragments arranged by size: 2219 1941 617 555 BsaAI yAC'GTr Cuts at: 493 1204 3123 493 Size: 711 1919 2702 Fragments arranged by size: 2702 1919 711 BsaBI GATnn'nnATC Cuts at: 5326 5326 Size: 5332 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3139 3521 4156 275 Size: 53 83 186 936 1606 382 635 1451 Fragments arranged by size: 1606 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2205 3765 4163 4437 4584 4995 2205 Size: 1560 398 274 147 411 2542 Fragments arranged by size: 2542 1560 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2513 2543 1178 Size: 30 165 30 1110 30 3967 Fragments arranged by size: 3967 1110 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2537 2540 1186 Size: 1351 3 3978 Fragments arranged by size: 3978 1351 3 BseYI C'CCAG_C Cuts at: 2194 4535 5031 2194 Size: 2341 496 2495 Fragments arranged by size: 2495 2341 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2456 1163 Size: 573 720 4039 Fragments arranged by size: 4039 720 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2782 3543 4571 1540 Size: 275 186 781 761 1028 2301 Fragments arranged by size: 2301 1028 781 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3393 3478 4549 3393 Size: 85 1071 4176 Fragments arranged by size: 4176 1071 85 BsmI GAATG_Cn' Cuts at: 2843 2936 2843 Size: 93 5239 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2651 3393 3478 4549 1391 Size: 399 280 581 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 581 399 280 85 BspEI T'CCGG_A Cuts at: 2205 4995 2205 Size: 2790 2542 BspHI T'CATG_A Cuts at: 3221 4951 3221 Size: 1730 3602 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2100 2687 844 Size: 1256 587 3489 Fragments arranged by size: 3489 1256 587 BsrBI CCG'CTC Cuts at: 1394 1823 2350 3219 1394 Size: 429 527 869 3507 Fragments arranged by size: 3507 869 527 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3827 4001 65 Size: 1183 2579 174 1396 Fragments arranged by size: 2579 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3976 4163 1700 Size: 110 2166 187 2869 Fragments arranged by size: 2869 2166 187 110 BsrGI T'GTAC_A Cuts at: 96 2595 96 Size: 2499 2833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5332 BssSI C'ACGA_G Cuts at: 3392 4404 3392 Size: 1012 4320 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5332 BstBI TT'CG_AA Cuts at: 4136 4136 Size: 5332 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5332 BstYI r'GATC_y Cuts at: 1972 2442 2541 2662 3029 3414 3431 4883 4969 Size: 470 99 121 367 385 17 1452 86 Cuts at: 4969 4981 5327 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 470 385 367 346 121 99 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2422 513 Size: 552 939 39 379 3423 Fragments arranged by size: 3423 939 552 379 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4593 Fragments arranged by size: 4593 683 56 BtsI GCAGTG_nn' Cuts at: 2848 3642 3662 2848 Size: 794 20 4518 Fragments arranged by size: 4518 794 20 ClaI AT'CG_AT Cuts at: 1554 2577 1554 Size: 1023 4309 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5297 DraI TTT'AAA Cuts at: 2636 2636 Size: 5332 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4339 809 Size: 507 3023 1802 Fragments arranged by size: 3023 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2779 3668 Size: 54 1425 50 275 18 186 763 889 Cuts at: 3668 5297 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 763 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2779 1537 Size: 275 967 4090 Fragments arranged by size: 4090 967 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2555 3262 1353 Size: 1202 707 3423 Fragments arranged by size: 3423 1202 707 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2534 3919 4293 4439 387 Size: 1064 138 945 1385 374 146 1280 Fragments arranged by size: 1385 1280 1064 945 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2246 2312 2555 3395 3992 4779 Size: 393 419 195 66 243 840 597 787 Cuts at: 4779 5047 1239 Size: 268 1524 Fragments arranged by size: 1524 840 787 597 419 393 268 243 195 66 EcoO109I rG'GnC_Cy Cuts at: 2067 2118 2067 Size: 51 5281 EcoRI G'AATT_C Cuts at: 2641 3019 2641 Size: 378 4954 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2500 2500 Size: 5332 FspI TGC'GCA Cuts at: 3838 3838 Size: 5332 HaeII r_GCGC'y Cuts at: 1678 2012 2285 2498 4479 1678 Size: 334 273 213 1981 2531 Fragments arranged by size: 2531 1981 334 273 213 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2069 2101 3973 4005 4047 Size: 32 135 32 497 32 1872 32 42 Cuts at: 4047 4079 1373 Size: 32 2626 Fragments arranged by size: 2626 1872 497 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2383 2560 2676 2922 669 Size: 988 281 445 177 116 246 3079 Fragments arranged by size: 3079 988 445 281 246 177 116 HindIII A'AGCT_T Cuts at: 748 748 Size: 5332 HpaI GTT'AAC Cuts at: 2922 2922 Size: 5332 KpnI G_GTAC'C Cuts at: 2657 2657 Size: 5332 MfeI C'AATT_G Cuts at: 2931 2931 Size: 5332 MluI A'CGCG_T Cuts at: 5208 5208 Size: 5332 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4446 4630 1305 Size: 3141 184 2007 Fragments arranged by size: 3141 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3510 Fragments arranged by size: 3510 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2603 3290 3649 3808 518 Size: 648 1437 687 359 159 2042 Fragments arranged by size: 2042 1437 687 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2045 2748 3425 4573 4818 1606 Size: 432 7 703 677 1148 245 2120 Fragments arranged by size: 2120 1148 703 677 432 245 7 NcoI C'CATG_G Cuts at: 513 1065 2004 2422 513 Size: 552 939 418 3423 Fragments arranged by size: 3423 939 552 418 NdeI CA'TA_TG Cuts at: 387 387 Size: 5332 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5332 NotI GC'GGCC_GC Cuts at: 2779 2779 Size: 5332 NspI r_CATG'y Cuts at: 1132 1229 2690 4235 1132 Size: 97 1461 1545 2229 Fragments arranged by size: 2229 1545 1461 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5332 PciI A'CATG_T Cuts at: 4231 4231 Size: 5332 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5332 PfoI T'CCnGG_A Cuts at: 2223 2223 Size: 5332 PmeI GTTT'AAAC Cuts at: 2636 2636 Size: 5332 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3401 3433 4914 4946 3401 Size: 32 1481 32 3787 Fragments arranged by size: 3787 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2118 2118 Size: 5332 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5332 PsiI TTA'TAA Cuts at: 2902 2902 Size: 5332 PspOMI G'GGCC_C Cuts at: 2066 2066 Size: 5332 PspXI vC'TCGA_Gb Cuts at: 2075 2075 Size: 5332 PstI C_TGCA'G Cuts at: 830 2217 2684 830 Size: 1387 467 3478 Fragments arranged by size: 3478 1387 467 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5332 SacII CC_GC'GG Cuts at: 2046 2046 Size: 5332 SalI G'TCGA_C Cuts at: 1936 2674 1936 Size: 738 4594 SanDI GG'GwC_CC Cuts at: 2118 2118 Size: 5332 SbfI CC_TGCA'GG Cuts at: 2684 2684 Size: 5332 ScaI AGT'ACT Cuts at: 3580 5292 3580 Size: 1712 3620 SfcI C'TryA_G Cuts at: 826 1045 2213 2680 3815 4496 4687 826 Size: 219 1168 467 1135 681 191 1471 Fragments arranged by size: 1471 1168 1135 681 467 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5332 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5332 SmaI CCC'GGG Cuts at: 1872 2659 1872 Size: 787 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2075 2411 3436 4337 4599 820 Size: 197 926 132 336 1025 901 262 1553 Fragments arranged by size: 1553 1025 926 901 336 262 197 132 SnaBI TAC'GTA Cuts at: 493 3123 493 Size: 2630 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5332 SphI G_CATG'C Cuts at: 1132 2690 1132 Size: 1558 3774 SspI AAT'ATT Cuts at: 5 52 3256 5 Size: 47 3204 2081 Fragments arranged by size: 3204 2081 47 StuI AGG'CCT Cuts at: 1517 2304 2396 1517 Size: 787 92 4453 Fragments arranged by size: 4453 787 92 StyI C'CwwG_G Cuts at: 513 1065 2004 2113 2391 2422 2502 2771 513 Size: 552 939 109 278 31 80 269 3074 Fragments arranged by size: 3074 939 552 278 269 109 80 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 3359 3376 3529 3714 4159 325 Size: 1255 1035 744 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1035 744 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 3578 5290 Size: 274 80 33 51 1443 618 983 1712 Cuts at: 5290 96 Size: 138 Fragments arranged by size: 1712 1443 983 618 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2513 2545 5292 5324 2513 Size: 32 2747 32 2521 Fragments arranged by size: 2747 2521 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5332 XbaI T'CTAG_A Cuts at: 2668 2668 Size: 5332 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5332 XhoI C'TCGA_G Cuts at: 1943 2075 1943 Size: 132 5200 XmaI C'CCGG_G Cuts at: 1870 2657 1870 Size: 787 4545 XmnI GAAnn'nnTTC Cuts at: 2444 3461 2444 Size: 1017 4315 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3140 276 Size: 53 83 186 936 1606 2468 Fragments arranged by size: 2468 1606 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI PvuII RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI