################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII AccIII AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI AspI AvrII BamHI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaWI BseAI BsiEI BsiWI BspEI BspLU11I BssHII Bst1107I BstBI BstZ17I CpoI DraI DrdI EagI EclXI Eco52I EcoRV EcoT22I FbaI FseI HindIII HpaI KspI MaeI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 2475 GGTACC G^GTACC AccI 1858 GTCTAC GT^[AC][GT]AC AcsI 1620 GAATTC [GA]^AATT[CT] AhdI 466 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1474 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2253 CCTGAGG CC^T[ACGT]AGG Aor51HI 2234 AGCGCT AGC^GCT ApoI 1620 GAATTC [GA]^AATT[CT] Asp700I 2259 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2475 GGTACC G^GTACC AspEI 466 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 200 TGCGCA TGC^GCA BbrPI 1275 CACGTG CAC^GTG BbvCI 418 CCTCAGC CCTCAGC(-5/-2) BsaBI 1146 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 1761 GAATGC GAATGC(1/-1) Bsp1407I 1313 TGTACA T^GTACA BspDI 247 ATCGAT AT^CGAT BsrGI 1313 TGTACA T^GTACA BstAPI 2321 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 2253 CCTGAGG CC^T[ACGT]AGG ClaI 247 ATCGAT AT^CGAT DraIII 754 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 466 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2234 AGCGCT AGC^GCT Eco81I 2253 CCTGAGG CC^T[ACGT]AGG EcoRI 1620 GAATTC G^AATTC FspI 200 TGCGCA TGC^GCA HincII 901 GTCAAC GT[CT]^[GA]AC HindII 901 GTCAAC GT[CT]^[GA]AC KpnI 2475 GGTACC GGTAC^C MamI 1146 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NruI 955 TCGCGA TCG^CGA PmaCI 1275 CACGTG CAC^GTG PmlI 1275 CACGTG CAC^GTG PstI 2203 CTGCAG CTGCA^G PvuII 1648 CAGCTG CAG^CTG SfiI 1716 GGCCTACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1051 CTCAAG C^T[CT][GA]AG SphI 975 GCATGC GCATG^C SspBI 1313 TGTACA T^GTACA Tsp509I 1621 AATT ^AATT XmnI 2259 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 1274 ACACGT A^C[GA][CT]GT 1276 ACGTGT ApaI 31 GGGCCC GGGCC^C 292 GGGCCC BbeI 217 GGCGCC GGCGC^C 944 GGCGCC BglI 167 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1717 GCCTACCTGGC BlpI 46 GCTGAGC GC^T[ACGT]AGC 2055 GCTGAGC Bpu1102I 46 GCTGAGC GC^T[ACGT]AGC 2055 GCTGAGC BsaAI 757 TACGTG [CT]AC^GT[GA] 1275 CACGTG BsmBI 1404 CGTCTC CGTCTC(1/5) 1683 CGTCTC BsrBI 2268 CCGCTC CCGCTC(-3/-3) 2608 CCGCTC BstYI 1151 GGATCT [GA]^GATC[CT] 1304 AGATCC CelII 46 GCTGAGC GC^T[ACGT]AGC 2055 GCTGAGC EcoNI 582 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2253 CCTGAGGAAGG HgaI 2602 GACGC GACGC(5/10) 741 GCGTC KasI 217 GGCGCC G^GCGCC 944 GGCGCC MflI 1151 GGATCT [GA]^GATC[CT] 1304 AGATCC MseI 680 TTAA T^TAA 2021 TTAA NarI 217 GGCGCC GG^CGCC 944 GGCGCC NcoI 308 CCATGG C^CATGG 1442 CCATGG SacI 49 GAGCTC GAGCT^C 2298 GAGCTC SapI 1551 GCTCTTC GCTCTTC(1/4) 1866 GCTCTTC SfcI 1803 CTATAG C^T[GA][CT]AG 2203 CTGCAG SfoI 217 GGCGCC GGC^GCC 944 GGCGCC Tru9I 680 TTAA T^TAA 2021 TTAA XhoII 1151 GGATCT [GA]^GATC[CT] 1304 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SmaI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XmaCI XmaI