(Linear) (Six-base) MAPSORT of: KIBB8516.seq Check: 8850 from: 1 to: 2613 KIBB8516.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2312 2613 Size: 2312 301 AccI GT'mk_AC Cuts at: 0 1859 2613 Size: 1859 754 Acc65I G'GTAC_C Cuts at: 0 2475 2613 Size: 2475 138 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1807 1871 1980 2316 2613 Size: 1807 64 109 336 297 Fragments arranged by size: 1807 336 297 109 64 AfeI AGC'GCT Cuts at: 0 2236 2613 Size: 2236 377 AflIII A'CryG_T Cuts at: 0 1274 1276 2613 Size: 1274 2 1337 Fragments arranged by size: 1337 1274 2 AhdI GACnn_n'nnGTC Cuts at: 0 471 2613 Size: 471 2142 AleI CACnn'nnGTG Cuts at: 0 350 1513 2119 2613 Size: 350 1163 606 494 Fragments arranged by size: 1163 606 494 350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 250 985 2613 Size: 250 735 1628 Fragments arranged by size: 1628 735 250 AlwNI CAG_nnn'CTG Cuts at: 0 1479 2613 Size: 1479 1134 ApaI G_GGCC'C Cuts at: 0 35 296 2613 Size: 35 261 2317 Fragments arranged by size: 2317 261 35 ApoI r'AATT_y Cuts at: 0 1620 2613 Size: 1620 993 AvaI C'yCGr_G Cuts at: 0 34 55 476 2394 2613 Size: 34 21 421 1918 219 Fragments arranged by size: 1918 421 219 34 21 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1480 1513 2613 Size: 1480 33 1100 Fragments arranged by size: 1480 1100 33 BanI G'GyrC_C Cuts at: 0 70 217 234 285 331 944 1199 2475 Size: 70 147 17 51 46 613 255 1276 Cuts at: 2475 2526 2613 Size: 51 87 Fragments arranged by size: 1276 613 255 147 87 70 51 51 46 17 BanII G_rGCy'C Cuts at: 0 35 53 296 403 484 1361 2302 2613 Size: 35 18 243 107 81 877 941 311 Fragments arranged by size: 941 877 311 243 107 81 35 18 BbeI G_GCGC'C Cuts at: 0 221 948 2613 Size: 221 727 1665 Fragments arranged by size: 1665 727 221 BbsI GAAGACnn'nnnn_ Cuts at: 0 435 580 2073 2613 Size: 435 145 1493 540 Fragments arranged by size: 1493 540 435 145 BbvCI CC'TCA_GC Cuts at: 0 419 2613 Size: 419 2194 BglI GCCn_nnn'nGGC Cuts at: 0 173 1723 2613 Size: 173 1550 890 Fragments arranged by size: 1550 890 173 BlpI GC'TnA_GC Cuts at: 0 47 2056 2613 Size: 47 2009 557 Fragments arranged by size: 2009 557 47 Bme1580I G_kGCm'C Cuts at: 0 35 239 296 1062 1489 1964 2613 Size: 35 204 57 766 427 475 649 Fragments arranged by size: 766 649 475 427 204 57 35 BmrI ACTGGGnnnn_n' Cuts at: 0 1233 1568 2613 Size: 1233 335 1045 Fragments arranged by size: 1233 1045 335 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 150 2613 Size: 150 2463 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 333 1105 1321 1501 1634 2068 2613 Size: 277 56 772 216 180 133 434 545 Fragments arranged by size: 772 545 434 277 216 180 133 56 Bpu10I CC'TnA_GC Cuts at: 0 419 604 2613 Size: 419 185 2009 Fragments arranged by size: 2009 419 185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1036 2613 Size: 1036 1577 BsaI GGTCTCn'nnnn_ Cuts at: 0 1431 1902 1984 2178 2360 2401 2613 Size: 1431 471 82 194 182 41 212 Fragments arranged by size: 1431 471 212 194 182 82 41 BsaAI yAC'GTr Cuts at: 0 759 1277 2613 Size: 759 518 1336 Fragments arranged by size: 1336 759 518 BsaBI GATnn'nnATC Cuts at: 0 1150 2613 Size: 1150 1463 BsaHI Gr'CG_yC Cuts at: 0 218 945 2603 2613 Size: 218 727 1658 10 Fragments arranged by size: 1658 727 218 10 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 773 803 1249 1279 1411 1441 2613 Size: 773 30 446 30 132 30 1172 Fragments arranged by size: 1172 773 446 132 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 90 562 1254 2119 2613 Size: 90 472 692 865 494 Fragments arranged by size: 865 692 494 472 90 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 709 864 1938 2613 Size: 709 155 1074 675 Fragments arranged by size: 1074 709 675 155 BsiHKAI G_wGCw'C Cuts at: 0 53 1097 2302 2613 Size: 53 1044 1205 311 Fragments arranged by size: 1205 1044 311 53 BsmI GAATG_Cn' Cuts at: 0 1767 2613 Size: 1767 846 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1410 1689 2613 Size: 1410 279 924 Fragments arranged by size: 1410 924 279 Bsp1286I G_dGCh'C Cuts at: 0 35 53 239 296 403 484 1062 1097 Size: 35 18 186 57 107 81 578 35 Cuts at: 1097 1361 1489 1964 2302 2613 Size: 264 128 475 338 311 Fragments arranged by size: 578 475 338 311 264 186 128 107 81 57 35 35 18 BspHI T'CATG_A Cuts at: 0 1121 1598 1811 1871 2613 Size: 1121 477 213 60 742 Fragments arranged by size: 1121 742 477 213 60 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 586 1049 1532 2312 2613 Size: 586 463 483 780 301 Fragments arranged by size: 780 586 483 463 301 BsrBI CCG'CTC Cuts at: 0 2270 2610 2613 Size: 2270 340 3 Fragments arranged by size: 2270 340 3 BsrDI GCAATG_nn' Cuts at: 0 615 989 1941 2141 2613 Size: 615 374 952 200 472 Fragments arranged by size: 952 615 472 374 200 BsrFI r'CCGG_y Cuts at: 0 150 449 658 2613 Size: 150 299 209 1955 Fragments arranged by size: 1955 299 209 150 BsrGI T'GTAC_A Cuts at: 0 1313 2613 Size: 1313 1300 BssSI C'ACGA_G Cuts at: 0 769 1339 1974 2613 Size: 769 570 635 639 Fragments arranged by size: 769 639 635 570 BstAPI GCAn_nnn'nTGC Cuts at: 0 2327 2613 Size: 2327 286 BstEII G'GTnAC_C Cuts at: 0 2097 2283 2575 2613 Size: 2097 186 292 38 Fragments arranged by size: 2097 292 186 38 BstXI CCAn_nnnn'nTGG Cuts at: 0 805 2037 2223 2422 2613 Size: 805 1232 186 199 191 Fragments arranged by size: 1232 805 199 191 186 BstYI r'GATC_y Cuts at: 0 1151 1304 2613 Size: 1151 153 1309 Fragments arranged by size: 1309 1151 153 Bsu36I CC'TnA_GG Cuts at: 0 2254 2613 Size: 2254 359 BtgI C'CryG_G Cuts at: 0 308 804 1442 1511 1779 2075 2288 2438 Size: 308 496 638 69 268 296 213 150 Cuts at: 2438 2613 Size: 175 Fragments arranged by size: 638 496 308 296 268 213 175 150 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 197 630 714 2573 2613 Size: 197 433 84 1859 40 Fragments arranged by size: 1859 433 197 84 40 BtsI GCAGTG_nn' Cuts at: 0 485 2212 2613 Size: 485 1727 401 Fragments arranged by size: 1727 485 401 ClaI AT'CG_AT Cuts at: 0 248 2613 Size: 248 2365 DraIII CAC_nnn'GTG Cuts at: 0 759 2613 Size: 759 1854 EaeI y'GGCC_r Cuts at: 0 107 612 986 1724 2562 2613 Size: 107 505 374 738 838 51 Fragments arranged by size: 838 738 505 374 107 51 EarI CTCTTCn'nnn_ Cuts at: 0 1072 1280 1558 1633 1834 1873 2613 Size: 1072 208 278 75 201 39 740 Fragments arranged by size: 1072 740 278 208 201 75 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 949 2613 Size: 19 930 1664 Fragments arranged by size: 1664 930 19 EcoICRI GAG'CTC Cuts at: 0 51 2300 2613 Size: 51 2249 313 Fragments arranged by size: 2249 313 51 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 333 1105 1321 1501 1634 1807 1871 Size: 277 56 772 216 180 133 173 64 Cuts at: 1871 1980 2068 2316 2613 Size: 109 88 248 297 Fragments arranged by size: 772 297 277 248 216 180 173 133 109 88 64 56 EcoNI CCTnn'n_nnAGG Cuts at: 0 586 2257 2613 Size: 586 1671 356 Fragments arranged by size: 1671 586 356 EcoO109I rG'GnC_Cy Cuts at: 0 19 31 1563 1799 1836 2391 2613 Size: 19 12 1532 236 37 555 222 Fragments arranged by size: 1532 555 236 222 37 19 12 EcoRI G'AATT_C Cuts at: 0 1620 2613 Size: 1620 993 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1845 2613 Size: 1845 768 FspI TGC'GCA Cuts at: 0 202 2613 Size: 202 2411 HaeII r_GCGC'y Cuts at: 0 221 813 948 2238 2613 Size: 221 592 135 1290 375 Fragments arranged by size: 1290 592 375 221 135 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 457 489 773 805 1096 1128 1249 1281 Size: 457 32 284 32 291 32 121 32 Cuts at: 1281 1838 1870 2449 2481 2613 Size: 557 32 579 32 132 Fragments arranged by size: 579 557 457 291 284 132 121 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 903 2613 Size: 903 1710 KasI G'GCGC_C Cuts at: 0 217 944 2613 Size: 217 727 1669 Fragments arranged by size: 1669 727 217 KpnI G_GTAC'C Cuts at: 0 2479 2613 Size: 2479 134 MscI TGG'CCA Cuts at: 0 109 988 2564 2613 Size: 109 879 1576 49 Fragments arranged by size: 1576 879 109 49 MslI CAynn'nnrTG Cuts at: 0 350 631 1420 1513 1927 2035 2119 2613 Size: 350 281 789 93 414 108 84 494 Fragments arranged by size: 789 494 414 350 281 108 93 84 MspA1I CmG'CkG Cuts at: 0 1479 1650 2555 2613 Size: 1479 171 905 58 Fragments arranged by size: 1479 905 171 58 NarI GG'CG_CC Cuts at: 0 218 945 2613 Size: 218 727 1668 Fragments arranged by size: 1668 727 218 NcoI C'CATG_G Cuts at: 0 308 1442 2613 Size: 308 1134 1171 Fragments arranged by size: 1171 1134 308 NruI TCG'CGA Cuts at: 0 957 2613 Size: 957 1656 NspI r_CATG'y Cuts at: 0 942 979 2139 2613 Size: 942 37 1160 474 Fragments arranged by size: 1160 942 474 37 PasI CC'CwG_GG Cuts at: 0 777 2613 Size: 777 1836 PflMI CCAn_nnn'nTGG Cuts at: 0 184 1479 2373 2613 Size: 184 1295 894 240 Fragments arranged by size: 1295 894 240 184 PfoI T'CCnGG_A Cuts at: 0 2009 2222 2613 Size: 2009 213 391 Fragments arranged by size: 2009 391 213 PmlI CAC'GTG Cuts at: 0 1277 2613 Size: 1277 1336 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2488 2520 2613 Size: 2488 32 93 Fragments arranged by size: 2488 93 32 PpuMI rG'GwC_Cy Cuts at: 0 1563 1799 1836 2391 2613 Size: 1563 236 37 555 222 Fragments arranged by size: 1563 555 236 222 37 PspOMI G'GGCC_C Cuts at: 0 31 292 2613 Size: 31 261 2321 Fragments arranged by size: 2321 261 31 PstI C_TGCA'G Cuts at: 0 2207 2613 Size: 2207 406 PvuII CAG'CTG Cuts at: 0 1650 2613 Size: 1650 963 SacI G_AGCT'C Cuts at: 0 53 2302 2613 Size: 53 2249 311 Fragments arranged by size: 2249 311 53 SapI GCTCTTCn'nnn_ Cuts at: 0 1558 1873 2613 Size: 1558 315 740 Fragments arranged by size: 1558 740 315 SfcI C'TryA_G Cuts at: 0 1803 2203 2613 Size: 1803 400 410 Fragments arranged by size: 1803 410 400 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1723 2613 Size: 1723 890 SfoI GGC'GCC Cuts at: 0 219 946 2613 Size: 219 727 1667 Fragments arranged by size: 1667 727 219 SmaI CCC'GGG Cuts at: 0 36 57 478 2396 2613 Size: 36 21 421 1918 217 Fragments arranged by size: 1918 421 217 36 21 SmlI C'TyrA_G Cuts at: 0 1051 2613 Size: 1051 1562 SphI G_CATG'C Cuts at: 0 979 2613 Size: 979 1634 StuI AGG'CCT Cuts at: 0 592 1291 1717 2263 2613 Size: 592 699 426 546 350 Fragments arranged by size: 699 592 546 426 350 StyI C'CwwG_G Cuts at: 0 308 587 824 1442 2177 2613 Size: 308 279 237 618 735 436 Fragments arranged by size: 735 618 436 308 279 237 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 321 2613 Size: 321 2292 TatI w'GTAC_w Cuts at: 0 1313 2613 Size: 1313 1300 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 252 492 2073 2220 2613 Size: 252 240 1581 147 393 Fragments arranged by size: 1581 393 252 240 147 XmaI C'CCGG_G Cuts at: 0 34 55 476 2394 2613 Size: 34 21 421 1918 219 Fragments arranged by size: 1918 421 219 34 21 XmnI GAAnn'nnTTC Cuts at: 0 2263 2613 Size: 2263 350 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApoI AvaI BaeI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I NarI NcoI NruI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SapI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TaqII TatI XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AflII AgeI AloI ApaLI AscI AseI AsiSI AvrII BamHI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BsaWI BseYI BsiEI BsiWI BspEI BssHII BstBI BstZ17I CspCI DraI DrdI EagI EcoRV FseI FspAI HindIII HpaI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI XhoI ZraI