(Circular) (Six-base) MAPSORT of: pFN21AE1884.seq Check: 5479 from: 1 to: 5935 pFN21AE1884 5935 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3745 278 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2328 3278 1937 Size: 391 950 4594 Fragments arranged by size: 4594 950 391 Acc65I G'GTAC_C Cuts at: 3256 3256 Size: 5935 AclI AA'CG_TT Cuts at: 1563 4062 4435 1563 Size: 2499 373 3063 Fragments arranged by size: 3063 2499 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2021 2106 2750 2900 3998 5382 5650 1632 Size: 389 85 644 150 1098 1384 268 1917 Fragments arranged by size: 1917 1384 1098 644 389 268 150 85 AflII C'TTAA_G Cuts at: 820 1017 2887 820 Size: 197 1870 3868 Fragments arranged by size: 3868 1870 197 AflIII A'CryG_T Cuts at: 1203 4834 5811 1203 Size: 3631 977 1327 Fragments arranged by size: 3631 1327 977 AgeI A'CCGG_T Cuts at: 4766 4766 Size: 5935 AhdI GACnn_n'nnGTC Cuts at: 4664 4664 Size: 5935 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5935 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5935 AlwNI CAG_nnn'CTG Cuts at: 2150 2354 3678 5250 2150 Size: 204 1324 1572 2835 Fragments arranged by size: 2835 1572 1324 204 ApaLI G'TGCA_C Cuts at: 3992 5148 3992 Size: 1156 4779 ApoI r'AATT_y Cuts at: 1493 2624 2712 2756 3177 3244 3470 3622 5731 Size: 1131 88 44 421 67 226 152 2109 Cuts at: 5731 1493 Size: 1697 Fragments arranged by size: 2109 1697 1131 421 226 152 88 67 44 AseI AT'TA_AT Cuts at: 160 1032 4489 160 Size: 872 3457 1606 Fragments arranged by size: 3457 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 AvaI C'yCGr_G Cuts at: 1870 1943 2425 2844 3260 1870 Size: 73 482 419 416 4545 Fragments arranged by size: 4545 482 419 416 73 BamHI G'GATC_C Cuts at: 3265 3632 3265 Size: 367 5568 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3256 4711 618 Size: 325 217 627 1469 1455 1842 Fragments arranged by size: 1842 1469 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2508 2851 3254 1391 Size: 1117 343 403 4072 Fragments arranged by size: 4072 1117 403 343 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4964 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4126 4160 1903 Size: 34 2189 34 3678 Fragments arranged by size: 3678 2189 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3827 5037 3827 Size: 1210 4725 BclI T'GATC_A Cuts at: 1548 2536 1548 Size: 988 4947 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4546 136 Size: 107 122 71 631 605 2874 1525 Fragments arranged by size: 2874 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2608 5930 2608 Size: 3322 2613 BlpI GC'TnA_GC Cuts at: 3352 3352 Size: 5935 Bme1580I G_kGCm'C Cuts at: 1790 3996 5152 1790 Size: 2206 1156 2573 Fragments arranged by size: 2573 2206 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5935 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3154 3666 4624 Size: 416 53 83 399 24 1737 512 958 Cuts at: 4624 442 Size: 1753 Fragments arranged by size: 1753 1737 958 512 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5935 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2599 4595 1239 Size: 1360 1996 2579 Fragments arranged by size: 2579 1996 1360 Bpu10I CC'TnA_GC Cuts at: 2154 2154 Size: 5935 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2031 4060 4925 5223 2031 Size: 2029 865 298 2743 Fragments arranged by size: 2743 2029 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4598 882 Size: 617 3099 2219 Fragments arranged by size: 3099 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3726 493 Size: 711 2522 2702 Fragments arranged by size: 2702 2522 711 BsaBI GATnn'nnATC Cuts at: 5929 5929 Size: 5935 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3742 4124 4759 275 Size: 53 83 186 936 2209 382 635 1451 Fragments arranged by size: 2209 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4368 4766 5040 5187 5598 4368 Size: 398 274 147 411 4705 Fragments arranged by size: 4705 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2727 2757 1178 Size: 30 165 30 1324 30 4356 Fragments arranged by size: 4356 1324 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3129 1186 Size: 1943 3992 BseYI C'CCAG_C Cuts at: 2143 5138 5634 2143 Size: 2995 496 2444 Fragments arranged by size: 2995 2444 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5362 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3385 4146 5174 1540 Size: 275 186 1384 761 1028 2301 Fragments arranged by size: 2301 1384 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2836 3996 4081 5152 2836 Size: 1160 85 1071 3619 Fragments arranged by size: 3619 1160 1071 85 BsmI GAATG_Cn' Cuts at: 3135 3446 3539 3135 Size: 311 93 5531 Fragments arranged by size: 5531 311 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2508 2836 2851 3254 3996 4081 5152 Size: 399 718 328 15 403 742 85 1071 Cuts at: 5152 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 718 403 399 328 85 15 BspEI T'CCGG_A Cuts at: 5598 5598 Size: 5935 BspHI T'CATG_A Cuts at: 3824 5554 3824 Size: 1730 4205 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3290 844 Size: 2446 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3822 1394 Size: 429 1999 3507 Fragments arranged by size: 3507 1999 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4430 4604 65 Size: 1183 3182 174 1396 Fragments arranged by size: 3182 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4579 4766 1700 Size: 110 2769 187 2869 Fragments arranged by size: 2869 2769 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5935 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5935 BssSI C'ACGA_G Cuts at: 3995 5007 3995 Size: 1012 4923 BstAPI GCAn_nnn'nTGC Cuts at: 794 2831 794 Size: 2037 3898 BstBI TT'CG_AA Cuts at: 4739 4739 Size: 5935 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5935 BstXI CCAn_nnnn'nTGG Cuts at: 2581 2581 Size: 5935 BstYI r'GATC_y Cuts at: 1972 2608 3265 3632 4017 4034 5486 5572 5584 Size: 636 657 367 385 17 1452 86 12 Cuts at: 5584 5930 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 657 636 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4444 Fragments arranged by size: 4444 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5196 Fragments arranged by size: 5196 683 56 BtsI GCAGTG_nn' Cuts at: 2691 3067 3155 3451 4245 4265 2691 Size: 376 88 296 794 20 4361 Fragments arranged by size: 4361 794 376 296 88 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5935 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5900 DraI TTT'AAA Cuts at: 2311 3239 2311 Size: 928 5007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4942 809 Size: 507 3626 1802 Fragments arranged by size: 3626 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3382 4271 5900 Size: 54 1425 50 275 18 1552 889 1629 Cuts at: 5900 8 Size: 43 Fragments arranged by size: 1629 1552 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3382 1537 Size: 275 1570 4090 Fragments arranged by size: 4090 1570 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3085 3865 1353 Size: 1732 780 3423 Fragments arranged by size: 3423 1732 780 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4522 4896 5042 387 Size: 1064 138 2933 374 146 1280 Fragments arranged by size: 2933 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2021 2106 2599 2750 2900 3998 4595 Size: 393 389 85 493 151 150 1098 597 Cuts at: 4595 5382 5650 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1098 787 597 493 393 389 268 151 150 85 EcoRI G'AATT_C Cuts at: 3244 3622 3244 Size: 378 5557 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2375 2375 Size: 5935 FspI TGC'GCA Cuts at: 4441 4441 Size: 5935 HaeII r_GCGC'y Cuts at: 1678 5082 1678 Size: 3404 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2356 2380 2388 2412 4576 Size: 32 135 32 784 24 8 24 2164 Cuts at: 4576 4608 4650 4682 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2164 784 135 42 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 3279 3525 669 Size: 988 281 1341 246 3079 Fragments arranged by size: 3079 1341 988 281 246 HindIII A'AGCT_T Cuts at: 748 2049 748 Size: 1301 4634 HpaI GTT'AAC Cuts at: 3525 3525 Size: 5935 KpnI G_GTAC'C Cuts at: 3260 3260 Size: 5935 MfeI C'AATT_G Cuts at: 3534 3534 Size: 5935 MluI A'CGCG_T Cuts at: 5811 5811 Size: 5935 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5049 5233 1305 Size: 3744 184 2007 Fragments arranged by size: 3744 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4113 Fragments arranged by size: 4113 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2532 3893 4252 4411 518 Size: 648 1366 1361 359 159 2042 Fragments arranged by size: 2042 1366 1361 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2521 3351 4028 5176 5421 1606 Size: 915 830 677 1148 245 2120 Fragments arranged by size: 2120 1148 915 830 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4444 Fragments arranged by size: 4444 939 552 NdeI CA'TA_TG Cuts at: 387 2529 3028 387 Size: 2142 499 3294 Fragments arranged by size: 3294 2142 499 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5935 NotI GC'GGCC_GC Cuts at: 3382 3382 Size: 5935 NspI r_CATG'y Cuts at: 1132 1229 3293 4838 1132 Size: 97 2064 1545 2229 Fragments arranged by size: 2229 2064 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5935 PciI A'CATG_T Cuts at: 4834 4834 Size: 5935 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5935 PmeI GTTT'AAAC Cuts at: 3239 3239 Size: 5935 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4004 4036 5517 5549 4004 Size: 32 1481 32 4390 Fragments arranged by size: 4390 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5935 PsiI TTA'TAA Cuts at: 3505 3505 Size: 5935 PspXI vC'TCGA_Gb Cuts at: 2844 2844 Size: 5935 PstI C_TGCA'G Cuts at: 830 3287 830 Size: 2457 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5935 PvuII CAG'CTG Cuts at: 2521 2521 Size: 5935 SalI G'TCGA_C Cuts at: 1936 3277 1936 Size: 1341 4594 SbfI CC_TGCA'GG Cuts at: 3287 3287 Size: 5935 ScaI AGT'ACT Cuts at: 4183 5895 4183 Size: 1712 4223 SfcI C'TryA_G Cuts at: 826 1045 2954 3283 4418 5099 5290 826 Size: 219 1909 329 1135 681 191 1471 Fragments arranged by size: 1909 1471 1135 681 329 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5935 SmaI CCC'GGG Cuts at: 1872 2427 3262 1872 Size: 555 835 4545 Fragments arranged by size: 4545 835 555 SmlI C'TyrA_G Cuts at: 820 1017 1943 2046 2844 2887 4039 4940 5202 Size: 197 926 103 798 43 1152 901 262 Cuts at: 5202 820 Size: 1553 Fragments arranged by size: 1553 1152 926 901 798 262 197 103 43 SnaBI TAC'GTA Cuts at: 493 3726 493 Size: 3233 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5935 SphI G_CATG'C Cuts at: 1132 3293 1132 Size: 2161 3774 SspI AAT'ATT Cuts at: 5 52 3859 5 Size: 47 3807 2081 Fragments arranged by size: 3807 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5935 StyI C'CwwG_G Cuts at: 513 1065 2004 3374 513 Size: 552 939 1370 3074 Fragments arranged by size: 3074 1370 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3962 3979 4132 4317 4762 325 Size: 1255 2382 17 153 185 445 1498 Fragments arranged by size: 2382 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2650 4181 5893 Size: 274 80 33 51 1443 673 1531 1712 Cuts at: 5893 96 Size: 138 Fragments arranged by size: 1712 1531 1443 673 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5895 5927 5895 Size: 32 5903 Tth111I GACn'n_nGTC Cuts at: 1316 2405 3160 1316 Size: 1089 755 4091 Fragments arranged by size: 4091 1089 755 XbaI T'CTAG_A Cuts at: 3271 3271 Size: 5935 XhoI C'TCGA_G Cuts at: 1943 2844 1943 Size: 901 5034 XmaI C'CCGG_G Cuts at: 1870 2425 3260 1870 Size: 555 835 4545 Fragments arranged by size: 4545 835 555 XmnI GAAnn'nnTTC Cuts at: 2133 4064 2133 Size: 1931 4004 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3743 276 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI