################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaWI BseAI BseRI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI Bsu36I CelII Cfr10I ClaI CpoI DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KasI KspI MluI MluNI MroI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 911 GGTACC G^GTACC AcyI 1206 GGCGTC G[GA]^CG[CT]C AlwNI 1085 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 1069 ATTAAT AT^TAAT AsnI 1069 ATTAAT AT^TAAT Asp718I 911 GGTACC G^GTACC BbsI 304 GTCTTC GAAGAC(2/6) BciVI 1607 GGATAC GTATCC(6/5) BcnI 312 CCCGG CC^[GC]GG BglII 1098 AGATCT A^GATCT BpmI 1062 CTCCAG CTGGAG(16/14) BpuAI 304 GTCTTC GAAGAC(2/6) BsaAI 43 TACGTG [CT]AC^GT[GA] BsaBI 596 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1206 GGCGTC G[GA]^CG[CT]C BsaI 1136 GGTCTC GGTCTC(1/5) BsmBI 382 CGTCTC CGTCTC(1/5) Bst1107I 423 GTATAC GTA^TAC BstYI 1098 AGATCT [GA]^GATC[CT] BstZ17I 423 GTATAC GTA^TAC DraI 651 TTTAAA TTT^AAA DraII 745 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 533 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 400 CTCTTC CTCTTC(1/4) EcoO109I 745 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 1426 GATATC GAT^ATC FauI 55 GCGGG CCCGC(4/6) HaeII 171 GGCGCT [GA]GCGC^[CT] HapII 313 CCGG C^CGG HgaI 1207 GCGTC GACGC(5/10) Hin1I 1206 GGCGTC G[GA]^CG[CT]C HincII 775 GTCAAC GT[CT]^[GA]AC HindII 775 GTCAAC GT[CT]^[GA]AC HpaII 313 CCGG C^CGG KpnI 911 GGTACC GGTAC^C Ksp632I 400 CTCTTC CTCTTC(1/4) MaeII 44 ACGT A^CGT MamI 596 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1316 CAATTG C^AATTG MflI 1098 AGATCT [GA]^GATC[CT] MspI 313 CCGG C^CGG MunI 1316 CAATTG C^AATTG NciI 312 CCCGG CC^[GC]GG NheI 187 GCTAGC G^CTAGC NotI 143 GCGGCCGC GC^GGCCGC PpuMI 745 GGGACCT [GA]G^G[AT]CC[CT] PshBI 1069 ATTAAT AT^TAAT SacI 1376 GAGCTC GAGCT^C SpeI 1495 ACTAGT A^CTAGT SphI 59 GCATGC GCATG^C TaqI 363 TCGA T^CGA TthHB8I 363 TCGA T^CGA XhoII 1098 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AccI 232 GTCTAC GT^[AC][GT]AC 423 GTATAC AlwI 925 GATCC GGATC(4/5) 581 GGATC AspHI 1322 GAGCAC G[AT]GC[AT]^C 1376 GAGCTC BfaI 188 CTAG C^TAG 1496 CTAG BsiEI 74 CGGCCG CG[GA][CT]^CG 144 CGGCCG BsiHKAI 1322 GAGCAC G[AT]GC[AT]^C 1376 GAGCTC BstF5I 602 CATCC GGATG(2/0) 1738 CATCC DsaI 10 CCACGG C^C[GA][CT]GG 167 CCGTGG EagI 74 CGGCCG C^GGCCG 144 CGGCCG EclXI 74 CGGCCG C^GGCCG 144 CGGCCG Eco52I 74 CGGCCG C^GGCCG 144 CGGCCG FokI 602 CATCC GGATG(9/13) 1738 CATCC MaeI 188 CTAG C^TAG 1496 CTAG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI AvaII BanI BanII BbvI BmyI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MslI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI