(Linear) (Six-base) MAPSORT of: KIEE6493.seq Check: 4633 from: 1 to: 1830 KIEE6493.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AccI GT'mk_AC Cuts at: 0 233 424 1830 Size: 233 191 1406 Fragments arranged by size: 1406 233 191 Acc65I G'GTAC_C Cuts at: 0 911 1830 Size: 911 919 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1101 1554 1830 Size: 1101 453 276 Fragments arranged by size: 1101 453 276 AleI CACnn'nnGTG Cuts at: 0 28 1830 Size: 28 1802 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1749 1781 1830 Size: 1749 32 49 Fragments arranged by size: 1749 49 32 AlwNI CAG_nnn'CTG Cuts at: 0 1090 1830 Size: 1090 740 ApoI r'AATT_y Cuts at: 0 681 690 1461 1830 Size: 681 9 771 369 Fragments arranged by size: 771 681 369 9 AseI AT'TA_AT Cuts at: 0 1070 1830 Size: 1070 760 BanI G'GyrC_C Cuts at: 0 22 217 911 1830 Size: 22 195 694 919 Fragments arranged by size: 919 694 195 22 BanII G_rGCy'C Cuts at: 0 896 1182 1380 1830 Size: 896 286 198 450 Fragments arranged by size: 896 450 286 198 BbsI GAAGACnn'nnnn_ Cuts at: 0 297 1830 Size: 297 1533 BciVI GTATCCnnnnn_n' Cuts at: 0 1601 1830 Size: 1601 229 BglII A'GATC_T Cuts at: 0 1098 1830 Size: 1098 732 Bme1580I G_kGCm'C Cuts at: 0 167 220 1830 Size: 167 53 1610 Fragments arranged by size: 1610 167 53 BmrI ACTGGGnnnn_n' Cuts at: 0 337 544 1830 Size: 337 207 1286 Fragments arranged by size: 1286 337 207 BmtI G_CTAG'C Cuts at: 0 191 1830 Size: 191 1639 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1047 1830 Size: 1047 783 Bpu10I CC'TnA_GC Cuts at: 0 1077 1830 Size: 1077 753 BsaI GGTCTCn'nnnn_ Cuts at: 0 1142 1830 Size: 1142 688 BsaAI yAC'GTr Cuts at: 0 45 1830 Size: 45 1785 BsaBI GATnn'nnATC Cuts at: 0 600 1830 Size: 600 1230 BsaHI Gr'CG_yC Cuts at: 0 1207 1830 Size: 1207 623 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1313 1343 1830 Size: 1313 30 487 Fragments arranged by size: 1313 487 30 BseYI C'CCAG_C Cuts at: 0 103 1830 Size: 103 1727 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 501 513 811 935 1830 Size: 501 12 298 124 895 Fragments arranged by size: 895 501 298 124 12 BsiEI CG_ry'CG Cuts at: 0 77 147 1830 Size: 77 70 1683 Fragments arranged by size: 1683 77 70 BsiHKAI G_wGCw'C Cuts at: 0 1326 1380 1830 Size: 1326 54 450 Fragments arranged by size: 1326 450 54 BsmBI CGTCTCn'nnnn_ Cuts at: 0 388 1830 Size: 388 1442 Bsp1286I G_dGCh'C Cuts at: 0 167 220 896 1182 1326 1380 1830 Size: 167 53 676 286 144 54 450 Fragments arranged by size: 676 450 286 167 144 54 53 BsrDI GCAATG_nn' Cuts at: 0 1408 1414 1821 1830 Size: 1408 6 407 9 Fragments arranged by size: 1408 407 9 6 BstYI r'GATC_y Cuts at: 0 1098 1830 Size: 1098 732 BstZ17I GTA'TAC Cuts at: 0 425 1830 Size: 425 1405 BtgI C'CryG_G Cuts at: 0 10 167 1830 Size: 10 157 1663 Fragments arranged by size: 1663 157 10 BtsI GCAGTG_nn' Cuts at: 0 167 1830 Size: 167 1663 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 491 526 1830 Size: 491 35 1304 Fragments arranged by size: 1304 491 35 DraI TTT'AAA Cuts at: 0 653 1830 Size: 653 1177 DraIII CAC_nnn'GTG Cuts at: 0 538 1830 Size: 538 1292 EaeI y'GGCC_r Cuts at: 0 74 111 144 1830 Size: 74 37 33 1686 Fragments arranged by size: 1686 74 37 33 EagI C'GGCC_G Cuts at: 0 74 144 1830 Size: 74 70 1686 Fragments arranged by size: 1686 74 70 EarI CTCTTCn'nnn_ Cuts at: 0 406 1830 Size: 406 1424 EcoICRI GAG'CTC Cuts at: 0 1378 1830 Size: 1378 452 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1047 1101 1554 1830 Size: 1047 54 453 276 Fragments arranged by size: 1047 453 276 54 EcoO109I rG'GnC_Cy Cuts at: 0 746 1830 Size: 746 1084 EcoRV GAT'ATC Cuts at: 0 1428 1830 Size: 1428 402 HaeII r_GCGC'y Cuts at: 0 175 1830 Size: 175 1655 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1669 1701 1830 Size: 1669 32 129 Fragments arranged by size: 1669 129 32 HincII GTy'rAC Cuts at: 0 777 1830 Size: 777 1053 KpnI G_GTAC'C Cuts at: 0 915 1830 Size: 915 915 MfeI C'AATT_G Cuts at: 0 1316 1830 Size: 1316 514 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1347 1560 1830 Size: 1347 213 270 Fragments arranged by size: 1347 270 213 MslI CAynn'nnrTG Cuts at: 0 28 920 1480 1830 Size: 28 892 560 350 Fragments arranged by size: 892 560 350 28 NheI G'CTAG_C Cuts at: 0 187 1830 Size: 187 1643 NotI GC'GGCC_GC Cuts at: 0 144 1830 Size: 144 1686 NspI r_CATG'y Cuts at: 0 63 878 1720 1830 Size: 63 815 842 110 Fragments arranged by size: 842 815 110 63 PfoI T'CCnGG_A Cuts at: 0 1002 1011 1830 Size: 1002 9 819 Fragments arranged by size: 1002 819 9 PpuMI rG'GwC_Cy Cuts at: 0 746 1830 Size: 746 1084 SacI G_AGCT'C Cuts at: 0 1380 1830 Size: 1380 450 SpeI A'CTAG_T Cuts at: 0 1495 1830 Size: 1495 335 SphI G_CATG'C Cuts at: 0 63 1830 Size: 63 1767 StyI C'CwwG_G Cuts at: 0 125 928 1155 1431 1830 Size: 125 803 227 276 399 Fragments arranged by size: 803 399 276 227 125 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 526 1830 Size: 526 1304 TatI w'GTAC_w Cuts at: 0 1565 1830 Size: 1565 265 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1007 1039 1151 1183 1830 Size: 1007 32 112 32 647 Fragments arranged by size: 1007 647 112 32 32 Enzymes that do cut: AccI Acc65I AcuI AleI AloI AlwNI ApoI AseI BanI BanII BbsI BciVI BglII Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrDI BstYI BstZ17I BtgI BtsI CspCI DraI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII KpnI MfeI MmeI MslI NheI NotI NspI PfoI PpuMI SacI SpeI SphI StyI TaqII TatI TstI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AhdI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI BplI BpuEI BsaWI BseRI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgZI ClaI DrdI EciI EcoNI EcoRI FalI FseI FspI FspAI HindIII HpaI KasI MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI