(Circular) (Six-base) MAPSORT of: pFN21AB6166.seq Check: 6775 from: 1 to: 6514 pFN21AB6166 6514 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2225 2292 2225 Size: 67 6447 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2374 4324 278 Size: 53 83 186 936 838 1950 2468 Fragments arranged by size: 2468 1950 936 838 186 83 53 AccI GT'mk_AC Cuts at: 1937 3857 1937 Size: 1920 4594 Acc65I G'GTAC_C Cuts at: 3835 3835 Size: 6514 AclI AA'CG_TT Cuts at: 1563 4641 5014 1563 Size: 3078 373 3063 Fragments arranged by size: 3078 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3511 3530 3720 4577 5961 6229 1632 Size: 1879 19 190 857 1384 268 1917 Fragments arranged by size: 1917 1879 1384 857 268 190 19 AflII C'TTAA_G Cuts at: 820 1017 2905 820 Size: 197 1888 4429 Fragments arranged by size: 4429 1888 197 AflIII A'CryG_T Cuts at: 1203 3121 5413 6390 1203 Size: 1918 2292 977 1327 Fragments arranged by size: 2292 1918 1327 977 AgeI A'CCGG_T Cuts at: 5345 5345 Size: 6514 AhdI GACnn_n'nnGTC Cuts at: 5243 5243 Size: 6514 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6514 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6514 AlwNI CAG_nnn'CTG Cuts at: 4257 5829 4257 Size: 1572 4942 ApaLI G'TGCA_C Cuts at: 3173 4571 5727 3173 Size: 1398 1156 3960 Fragments arranged by size: 3960 1398 1156 ApoI r'AATT_y Cuts at: 1493 2434 2933 3572 3596 3823 4049 4201 6310 Size: 941 499 639 24 227 226 152 2109 Cuts at: 6310 1493 Size: 1697 Fragments arranged by size: 2109 1697 941 639 499 227 226 152 24 AseI AT'TA_AT Cuts at: 160 1032 2475 5068 160 Size: 872 1443 2593 1606 Fragments arranged by size: 2593 1606 1443 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6514 AvaI C'yCGr_G Cuts at: 1870 1943 3839 1870 Size: 73 1896 4545 Fragments arranged by size: 4545 1896 73 BamHI G'GATC_C Cuts at: 3844 4211 3844 Size: 367 6147 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3835 5290 618 Size: 325 217 627 2048 1455 1842 Fragments arranged by size: 2048 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3029 3833 1391 Size: 1638 804 4072 Fragments arranged by size: 4072 1638 804 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3368 3380 928 Size: 971 1469 12 4062 Fragments arranged by size: 4062 1469 971 12 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4705 4739 1903 Size: 34 2768 34 3678 Fragments arranged by size: 3678 2768 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3485 4406 5616 3485 Size: 921 1210 4383 Fragments arranged by size: 4383 1210 921 BclI T'GATC_A Cuts at: 1548 2110 1548 Size: 562 5952 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5125 136 Size: 107 122 71 631 605 3453 1525 Fragments arranged by size: 3453 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2863 6509 2863 Size: 3646 2868 BlpI GC'TnA_GC Cuts at: 3931 3931 Size: 6514 Bme1580I G_kGCm'C Cuts at: 1790 3177 3229 4575 5731 1790 Size: 1387 52 1346 1156 2573 Fragments arranged by size: 2573 1387 1346 1156 52 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6514 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2088 3232 3316 Size: 416 53 83 399 24 671 1144 84 Cuts at: 3316 4245 5203 442 Size: 929 958 1753 Fragments arranged by size: 1753 1144 958 929 671 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6514 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3211 3211 Size: 6514 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2208 2292 2766 3024 3087 3181 5174 1239 Size: 969 84 474 258 63 94 1993 2579 Fragments arranged by size: 2579 1993 969 474 258 94 84 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4639 5504 5802 4639 Size: 865 298 5351 Fragments arranged by size: 5351 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3725 5177 882 Size: 617 2226 1452 2219 Fragments arranged by size: 2226 2219 1452 617 BsaAI yAC'GTr Cuts at: 493 1204 4305 493 Size: 711 3101 2702 Fragments arranged by size: 3101 2702 711 BsaBI GATnn'nnATC Cuts at: 6508 6508 Size: 6514 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2371 4321 4703 5338 Size: 53 83 186 936 838 1950 382 635 Cuts at: 5338 275 Size: 1451 Fragments arranged by size: 1950 1451 936 838 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4947 5345 5619 5766 6177 4947 Size: 398 274 147 411 5284 Fragments arranged by size: 5284 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2046 2076 2647 2677 1178 Size: 30 165 30 643 30 571 30 5015 Fragments arranged by size: 5015 643 571 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6514 BseYI C'CCAG_C Cuts at: 5717 6213 5717 Size: 496 6018 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2259 1163 Size: 573 523 5418 Fragments arranged by size: 5418 573 523 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3964 4725 5753 1540 Size: 275 186 1963 761 1028 2301 Fragments arranged by size: 2301 1963 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3014 3029 3177 3438 4575 4660 5731 3014 Size: 15 148 261 1137 85 1071 3797 Fragments arranged by size: 3797 1137 1071 261 148 85 15 BsmI GAATG_Cn' Cuts at: 3691 4025 4118 3691 Size: 334 93 6087 Fragments arranged by size: 6087 334 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3014 3029 3177 3229 3438 3833 4575 Size: 399 1224 15 148 52 209 395 742 Cuts at: 4575 4660 5731 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1224 1071 742 399 395 209 148 85 52 15 BspEI T'CCGG_A Cuts at: 6177 6177 Size: 6514 BspHI T'CATG_A Cuts at: 2727 4403 6133 2727 Size: 1676 1730 3108 Fragments arranged by size: 3108 1730 1676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2225 2292 2499 3869 844 Size: 1381 67 207 1370 3489 Fragments arranged by size: 3489 1381 1370 207 67 BsrBI CCG'CTC Cuts at: 1394 1823 4401 1394 Size: 429 2578 3507 Fragments arranged by size: 3507 2578 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2917 5009 5183 65 Size: 1183 1669 2092 174 1396 Fragments arranged by size: 2092 1669 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5158 5345 1700 Size: 110 3348 187 2869 Fragments arranged by size: 3348 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3052 96 Size: 2956 3558 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6514 BssSI C'ACGA_G Cuts at: 2583 4574 5586 2583 Size: 1991 1012 3511 Fragments arranged by size: 3511 1991 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3018 794 Size: 2224 4290 BstBI TT'CG_AA Cuts at: 5318 5318 Size: 6514 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6514 BstYI r'GATC_y Cuts at: 1972 2863 3844 4211 4596 4613 6065 6151 6163 Size: 891 981 367 385 17 1452 86 12 Cuts at: 6163 6509 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 981 891 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5023 Fragments arranged by size: 5023 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5775 Fragments arranged by size: 5775 683 56 BtsI GCAGTG_nn' Cuts at: 4030 4824 4844 4030 Size: 794 20 5700 Fragments arranged by size: 5700 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6514 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3744 3779 529 Size: 35 3180 35 3264 Fragments arranged by size: 3264 3180 35 35 DraI TTT'AAA Cuts at: 2416 3818 2416 Size: 1402 5112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5521 809 Size: 507 4205 1802 Fragments arranged by size: 4205 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 2820 3961 Size: 54 1425 50 275 18 351 639 1141 Cuts at: 3961 4850 6479 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1141 889 639 351 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3961 1537 Size: 275 2149 4090 Fragments arranged by size: 4090 2149 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3530 4444 1353 Size: 2177 914 3423 Fragments arranged by size: 3423 2177 914 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5101 5475 5621 387 Size: 1064 138 3512 374 146 1280 Fragments arranged by size: 3512 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3027 3027 Size: 6514 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2208 2292 2766 3024 3087 3181 3511 Size: 393 576 84 474 258 63 94 330 Cuts at: 3511 3530 3720 4577 5174 5961 6229 1239 Size: 19 190 857 597 787 268 1524 Fragments arranged by size: 1524 857 787 597 576 474 393 330 268 258 190 94 84 63 19 EcoO109I rG'GnC_Cy Cuts at: 2638 2638 Size: 6514 EcoRI G'AATT_C Cuts at: 3596 3823 4201 3596 Size: 227 378 5909 Fragments arranged by size: 5909 378 227 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2231 2231 Size: 6514 FspI TGC'GCA Cuts at: 5020 5020 Size: 6514 HaeII r_GCGC'y Cuts at: 1678 5661 1678 Size: 3983 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2348 2380 2906 2938 3389 Size: 32 135 32 776 32 526 32 451 Cuts at: 3389 3421 3642 3674 3773 3805 5155 5187 5229 Size: 32 221 32 99 32 1350 32 42 Cuts at: 5229 5261 1373 Size: 32 2626 Fragments arranged by size: 2626 1350 776 526 451 221 135 99 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2242 2700 3408 3858 4104 669 Size: 988 281 304 458 708 450 246 3079 Fragments arranged by size: 3079 988 708 458 450 304 281 246 HindIII A'AGCT_T Cuts at: 748 2902 3285 748 Size: 2154 383 3977 Fragments arranged by size: 3977 2154 383 HpaI GTT'AAC Cuts at: 4104 4104 Size: 6514 KpnI G_GTAC'C Cuts at: 3839 3839 Size: 6514 MfeI C'AATT_G Cuts at: 3370 4113 3370 Size: 743 5771 MluI A'CGCG_T Cuts at: 6390 6390 Size: 6514 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2622 2690 5628 5812 1305 Size: 1317 68 2938 184 2007 Fragments arranged by size: 2938 2007 1317 184 68 MscI TGG'CCA Cuts at: 10 64 1489 1832 2183 2822 10 Size: 54 1425 343 351 639 3702 Fragments arranged by size: 3702 1425 639 351 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4472 4831 4990 518 Size: 648 3306 359 159 2042 Fragments arranged by size: 3306 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2496 2765 3930 4607 5755 6000 1606 Size: 890 269 1165 677 1148 245 2120 Fragments arranged by size: 2120 1165 1148 890 677 269 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5023 Fragments arranged by size: 5023 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6514 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6514 NotI GC'GGCC_GC Cuts at: 3961 3961 Size: 6514 NspI r_CATG'y Cuts at: 1132 1229 3125 3872 5417 1132 Size: 97 1896 747 1545 2229 Fragments arranged by size: 2229 1896 1545 747 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6514 PciI A'CATG_T Cuts at: 3121 5413 3121 Size: 2292 4222 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6514 PfoI T'CCnGG_A Cuts at: 2200 3001 2200 Size: 801 5713 PmeI GTTT'AAAC Cuts at: 3818 3818 Size: 6514 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4583 4615 6096 6128 4583 Size: 32 1481 32 4969 Fragments arranged by size: 4969 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2638 2638 Size: 6514 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6514 PsiI TTA'TAA Cuts at: 3786 4084 3786 Size: 298 6216 PstI C_TGCA'G Cuts at: 830 2289 2510 3781 3866 830 Size: 1459 221 1271 85 3478 Fragments arranged by size: 3478 1459 1271 221 85 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6514 PvuII CAG'CTG Cuts at: 2765 2765 Size: 6514 SacI G_AGCT'C Cuts at: 3029 3029 Size: 6514 SalI G'TCGA_C Cuts at: 1936 3856 1936 Size: 1920 4594 SbfI CC_TGCA'GG Cuts at: 3866 3866 Size: 6514 ScaI AGT'ACT Cuts at: 3522 4762 6474 3522 Size: 1240 1712 3562 Fragments arranged by size: 3562 1712 1240 SfcI C'TryA_G Cuts at: 826 1045 2285 2506 3449 3637 3777 3862 4997 Size: 219 1240 221 943 188 140 85 1135 Cuts at: 4997 5678 5869 826 Size: 681 191 1471 Fragments arranged by size: 1471 1240 1135 943 681 221 219 191 188 140 85 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6514 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6514 SmaI CCC'GGG Cuts at: 1872 3841 1872 Size: 1969 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2905 4618 5519 5781 820 Size: 197 926 962 1713 901 262 1553 Fragments arranged by size: 1713 1553 962 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4305 493 Size: 3812 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6514 SphI G_CATG'C Cuts at: 1132 3872 1132 Size: 2740 3774 SspI AAT'ATT Cuts at: 5 52 2734 4438 5 Size: 47 2682 1704 2081 Fragments arranged by size: 2682 2081 1704 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6514 StyI C'CwwG_G Cuts at: 513 1065 2004 2122 2314 3953 513 Size: 552 939 118 192 1639 3074 Fragments arranged by size: 3074 1639 939 552 192 118 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4541 4558 4711 4896 5341 325 Size: 1255 2961 17 153 185 445 1498 Fragments arranged by size: 2961 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2690 2813 3052 Size: 274 80 33 51 1443 713 123 239 Cuts at: 3052 3520 4760 6472 96 Size: 468 1240 1712 138 Fragments arranged by size: 1712 1443 1240 713 468 274 239 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6474 6506 6474 Size: 32 6482 Tth111I GACn'n_nGTC Cuts at: 1316 2549 1316 Size: 1233 5281 XbaI T'CTAG_A Cuts at: 3850 3850 Size: 6514 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6514 XmaI C'CCGG_G Cuts at: 1870 3839 1870 Size: 1969 4545 XmnI GAAnn'nnTTC Cuts at: 4643 4643 Size: 6514 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2372 4322 276 Size: 53 83 186 936 838 1950 2468 Fragments arranged by size: 2468 1950 936 838 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI