(Linear) (Six-base) MAPSORT of: KIBB6166.seq Check: 3248 from: 1 to: 1809 KIBB6166.orf With 182 enzymes: SgfI * July 15, 2010 18:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 220 287 1809 Size: 220 67 1522 Fragments arranged by size: 1522 220 67 AatII G_ACGT'C Cuts at: 0 369 1809 Size: 369 1440 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1506 1525 1715 1809 Size: 1506 19 190 94 Fragments arranged by size: 1506 190 94 19 AflII C'TTAA_G Cuts at: 0 900 1809 Size: 900 909 AflIII A'CryG_T Cuts at: 0 1116 1809 Size: 1116 693 ApaLI G'TGCA_C Cuts at: 0 1168 1809 Size: 1168 641 ApoI r'AATT_y Cuts at: 0 429 928 1567 1591 1809 Size: 429 499 639 24 218 Fragments arranged by size: 639 499 429 218 24 AseI AT'TA_AT Cuts at: 0 470 1809 Size: 470 1339 BanII G_rGCy'C Cuts at: 0 1024 1809 Size: 1024 785 BbsI GAAGACnn'nnnn_ Cuts at: 0 1363 1375 1809 Size: 1363 12 434 Fragments arranged by size: 1363 434 12 BciVI GTATCCnnnnn_n' Cuts at: 0 1480 1809 Size: 1480 329 BclI T'GATC_A Cuts at: 0 105 1809 Size: 105 1704 BglII A'GATC_T Cuts at: 0 858 1809 Size: 858 951 Bme1580I G_kGCm'C Cuts at: 0 1172 1224 1809 Size: 1172 52 585 Fragments arranged by size: 1172 585 52 BmrI ACTGGGnnnn_n' Cuts at: 0 83 1227 1311 1809 Size: 83 1144 84 498 Fragments arranged by size: 1144 498 84 83 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1206 1809 Size: 1206 603 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 203 287 761 1019 1082 1176 1809 Size: 203 84 474 258 63 94 633 Fragments arranged by size: 633 474 258 203 94 84 63 BsaI GGTCTCn'nnnn_ Cuts at: 0 1720 1809 Size: 1720 89 BsaHI Gr'CG_yC Cuts at: 0 366 1809 Size: 366 1443 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 41 71 642 672 1809 Size: 41 30 571 30 1137 Fragments arranged by size: 1137 571 41 30 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 254 1809 Size: 254 1555 BsiHKAI G_wGCw'C Cuts at: 0 1009 1024 1172 1433 1809 Size: 1009 15 148 261 376 Fragments arranged by size: 1009 376 261 148 15 BsmI GAATG_Cn' Cuts at: 0 1686 1809 Size: 1686 123 Bsp1286I G_dGCh'C Cuts at: 0 1009 1024 1172 1224 1433 1809 Size: 1009 15 148 52 209 376 Fragments arranged by size: 1009 376 209 148 52 15 BspHI T'CATG_A Cuts at: 0 722 1809 Size: 722 1087 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 220 287 494 1809 Size: 220 67 207 1315 Fragments arranged by size: 1315 220 207 67 BsrDI GCAATG_nn' Cuts at: 0 912 1809 Size: 912 897 BsrGI T'GTAC_A Cuts at: 0 1047 1809 Size: 1047 762 BssSI C'ACGA_G Cuts at: 0 578 1809 Size: 578 1231 BstAPI GCAn_nnn'nTGC Cuts at: 0 1013 1809 Size: 1013 796 BstYI r'GATC_y Cuts at: 0 858 1809 Size: 858 951 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1739 1774 1809 Size: 1739 35 35 Fragments arranged by size: 1739 35 35 DraI TTT'AAA Cuts at: 0 411 1809 Size: 411 1398 EaeI y'GGCC_r Cuts at: 0 176 815 1809 Size: 176 639 994 Fragments arranged by size: 994 639 176 EarI CTCTTCn'nnn_ Cuts at: 0 1525 1809 Size: 1525 284 EcoICRI GAG'CTC Cuts at: 0 1022 1809 Size: 1022 787 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 203 287 761 1019 1082 1176 1506 1525 Size: 203 84 474 258 63 94 330 19 Cuts at: 1525 1715 1809 Size: 190 94 Fragments arranged by size: 474 330 258 203 190 94 94 84 63 19 EcoO109I rG'GnC_Cy Cuts at: 0 633 1809 Size: 633 1176 EcoRI G'AATT_C Cuts at: 0 1591 1809 Size: 1591 218 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 226 1809 Size: 226 1583 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 343 375 901 933 1384 1416 1637 1669 Size: 343 32 526 32 451 32 221 32 Cuts at: 1669 1768 1800 1809 Size: 99 32 9 Fragments arranged by size: 526 451 343 221 99 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 0 237 695 1403 1809 Size: 237 458 708 406 Fragments arranged by size: 708 458 406 237 HindIII A'AGCT_T Cuts at: 0 897 1280 1809 Size: 897 383 529 Fragments arranged by size: 897 529 383 MfeI C'AATT_G Cuts at: 0 1365 1809 Size: 1365 444 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 617 685 1809 Size: 617 68 1124 Fragments arranged by size: 1124 617 68 MscI TGG'CCA Cuts at: 0 178 817 1809 Size: 178 639 992 Fragments arranged by size: 992 639 178 MspA1I CmG'CkG Cuts at: 0 491 760 1809 Size: 491 269 1049 Fragments arranged by size: 1049 491 269 NspI r_CATG'y Cuts at: 0 1120 1809 Size: 1120 689 PciI A'CATG_T Cuts at: 0 1116 1809 Size: 1116 693 PfoI T'CCnGG_A Cuts at: 0 195 996 1809 Size: 195 801 813 Fragments arranged by size: 813 801 195 PpuMI rG'GwC_Cy Cuts at: 0 633 1809 Size: 633 1176 PsiI TTA'TAA Cuts at: 0 1781 1809 Size: 1781 28 PstI C_TGCA'G Cuts at: 0 284 505 1776 1809 Size: 284 221 1271 33 Fragments arranged by size: 1271 284 221 33 PvuII CAG'CTG Cuts at: 0 760 1809 Size: 760 1049 SacI G_AGCT'C Cuts at: 0 1024 1809 Size: 1024 785 ScaI AGT'ACT Cuts at: 0 1517 1809 Size: 1517 292 SfcI C'TryA_G Cuts at: 0 280 501 1444 1632 1772 1809 Size: 280 221 943 188 140 37 Fragments arranged by size: 943 280 221 188 140 37 SmlI C'TyrA_G Cuts at: 0 900 1809 Size: 900 909 SspI AAT'ATT Cuts at: 0 729 1809 Size: 729 1080 StyI C'CwwG_G Cuts at: 0 117 309 1809 Size: 117 192 1500 Fragments arranged by size: 1500 192 117 TatI w'GTAC_w Cuts at: 0 685 808 1047 1515 1809 Size: 685 123 239 468 294 Fragments arranged by size: 685 468 294 239 123 Tth111I GACn'n_nGTC Cuts at: 0 544 1809 Size: 544 1265 ZraI GAC'GTC Cuts at: 0 367 1809 Size: 367 1442 Enzymes that do cut: AarI AatII AcuI AflII AflIII ApaLI ApoI AseI BanII BbsI BciVI BclI BglII Bme1580I BmrI BplI BpmI BsaI BsaHI BsaXI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI BstYI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII MfeI MmeI MscI MspA1I NspI PciI PfoI PpuMI PsiI PstI PvuII SacI ScaI SfcI SmlI SspI StyI TatI Tth111I ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BfrBI BglI BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BseRI BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI EagI EciI EcoNI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI XbaI XcmI XhoI XmaI XmnI