Restriction map for
pFN21AB7637
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AcyI AfaI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BamHI BbeI BbvCI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MroI MseI MslI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI PvuI PvuII RcaI
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tru9I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 172 CGCG CG^CG
AclI 572 AACGTT AA^CGTT
AhdI 106 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 236 GGATC GGATC(4/5)
Asp700I 807 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 106 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 579 TGGCCA TGG^CCA
BanI 401 GGCACC G^G[CT][GA]CC
BbsI 912 GTCTTC GAAGAC(2/6)
BcgI 15 CGAGAAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 379 GTATCC GTATCC(6/5)
BcnI 468 CCCGG CC^[GC]GG
BfaI 430 CTAG C^TAG
BpuAI 912 GTCTTC GAAGAC(2/6)
BseRI 433 GAGGAG GAGGAG(10/8)
BsgI 148 GTGCAG GTGCAG(16/14)
BsmBI 97 GAGACG CGTCTC(1/5)
BsrDI 374 GCAATG GCAATG(2/0)
BstBI 932 TTCGAA TT^CGAA
BstUI 172 CGCG CG^CG
DpnII 237 GATC ^GATC
EaeI 579 TGGCCA [CT]^GGCC[GA]
Eam1105I 106 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 851 CTCTTC CTCTTC(1/4)
FauI 170 CCCGC CCCGC(4/6)
HapII 469 CCGG C^CGG
HpaII 469 CCGG C^CGG
HphI 416 TCACC GGTGA(8/7)
Ksp632I 851 CTCTTC CTCTTC(1/4)
MaeI 430 CTAG C^TAG
MboI 237 GATC ^GATC
MluNI 579 TGGCCA TGG^CCA
MscI 579 TGGCCA TGG^CCA
MspI 469 CCGG C^CGG
MvnI 172 CGCG CG^CG
NciI 468 CCCGG CC^[GC]GG
NdeII 237 GATC ^GATC
NspV 932 TTCGAA TT^CGAA
Psp1406I 572 AACGTT AA^CGTT
PstI 134 CTGCAG CTGCA^G
SacI 508 GAGCTC GAGCT^C
Sau3AI 237 GATC ^GATC
SfcI 134 CTGCAG C^T[GA][CT]AG
SfuI 932 TTCGAA TT^CGAA
SmlI 28 CTCAAG C^T[CT][GA]AG
SspI 961 AATATT AAT^ATT
StuI 152 AGGCCT AGG^CCT
XmnI 807 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 171 CCGC CCGC(-3/-1)
956 CCGC
BanII 33 GAGCCC G[GA]GC[CT]^C
508 GAGCTC
BpmI 82 CTGGAG CTGGAG(16/14)
439 CTGGAG
BsiYI 316 CCCGTGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
317 CCGTGAAAGGG
BslI 316 CCCGTGAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
317 CCGTGAAAGGG
BstAPI 180 GCATCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
679 GCAGGAAATGC
BstNI 521 CCAGG CC^[AT]GG
528 CCAGG
DraII 75 AGGTCCT [GA]G^G[ACGT]CC[CT]
199 AGGACCT
EcoO109I 75 AGGTCCT [GA]G^G[ACGT]CC[CT]
199 AGGACCT
EcoRII 521 CCAGG ^CC[AT]GG
528 CCAGG
EcoT14I 637 CCAAGG C^C[AT][AT]GG
944 CCAAGG
EcoT22I 714 ATGCAT ATGCA^T
878 ATGCAT
HgaI 99 GACGC GACGC(5/10)
130 GCGTC
MvaI 521 CCAGG CC^[AT]GG
528 CCAGG
NsiI 714 ATGCAT ATGCA^T
878 ATGCAT
NspI 716 GCATGT [GA]CATG^[CT]
876 ACATGC
PleI 847 GACTC GAGTC(4/5)
112 GAGTC
PpuMI 75 AGGTCCT [GA]G^G[AT]CC[CT]
199 AGGACCT
SfaNI 327 GATGC GCATC(5/9)
180 GCATC
StyI 637 CCAAGG C^C[AT][AT]GG
944 CCAAGG
TaqI 546 TCGA T^CGA
933 TCGA
TfiI 660 GATTC G^A[AT]TC
775 GATTC
Tsp45I 53 GTGAC ^GT[GC]AC
728 GTGAC
Tsp509I 670 AATT ^AATT
835 AATT
TthHB8I 546 TCGA T^CGA
933 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI AspHI AvaII BbrPI BbvI
BmyI BsaAI BsaJI BsiHKAI BsmAI
Bsp1286I BsrI BssKI BstF5I Cac8I
CfoI Cfr13I DdeI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI ItaI
MaeII MaeIII MboII MnlI MwoI
NlaIII NlaIV PmaCI PmlI Sau96I
ScrFI TseI TspRI