(Circular) (Six-base) MAPSORT of: pFN21AB7637.seq Check: 5765 from: 1 to: 5683 pFN21AB7637 5683 bp With 182 enzymes: SgfI * March 28, 2012 12:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3026 1937 Size: 1089 4594 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 2578 3810 4183 1563 Size: 1015 1232 373 3063 Fragments arranged by size: 3063 1232 1015 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3746 5130 5398 1632 Size: 2114 1384 268 1917 Fragments arranged by size: 2114 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 4582 5559 1203 Size: 3379 977 1327 Fragments arranged by size: 3379 1327 977 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 2116 4412 2116 Size: 2296 3387 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2164 2168 1184 Size: 980 4 4699 Fragments arranged by size: 4699 980 4 AlwNI CAG_nnn'CTG Cuts at: 3426 4998 3426 Size: 1572 4111 ApaLI G'TGCA_C Cuts at: 3740 4896 3740 Size: 1156 4527 ApoI r'AATT_y Cuts at: 1493 2992 3218 3370 5479 1493 Size: 1499 226 152 2109 1697 Fragments arranged by size: 2109 1697 1499 226 152 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 3008 1870 Size: 73 1065 4545 Fragments arranged by size: 4545 1065 73 BamHI G'GATC_C Cuts at: 3013 3380 3013 Size: 367 5316 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2406 3004 4459 618 Size: 325 217 627 619 598 1455 1842 Fragments arranged by size: 1842 1455 627 619 598 325 217 BanII G_rGCy'C Cuts at: 1391 2042 2517 3002 1391 Size: 651 475 485 4072 Fragments arranged by size: 4072 651 485 475 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2910 928 Size: 971 1011 3701 Fragments arranged by size: 3701 1011 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2009 2043 3874 3908 1903 Size: 34 72 34 1831 34 3678 Fragments arranged by size: 3678 1831 72 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2395 3575 4785 2395 Size: 1180 1210 3293 Fragments arranged by size: 3293 1210 1180 BclI T'GATC_A Cuts at: 1548 1548 Size: 5683 BfrBI ATG'CAT Cuts at: 2721 2885 2721 Size: 164 5519 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4294 136 Size: 107 122 71 631 605 2622 1525 Fragments arranged by size: 2622 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 3100 3100 Size: 5683 Bme1580I G_kGCm'C Cuts at: 1790 2832 3744 4900 1790 Size: 1042 912 1156 2573 Fragments arranged by size: 2573 1156 1042 912 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5683 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2874 3414 4372 Size: 416 53 83 399 24 1457 540 958 Cuts at: 4372 442 Size: 1753 Fragments arranged by size: 1753 1457 958 540 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2224 2224 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2465 4343 1239 Size: 869 357 1878 2579 Fragments arranged by size: 2579 1878 869 357 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2018 3808 4673 4971 2018 Size: 1790 865 298 2730 Fragments arranged by size: 2730 1790 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4346 882 Size: 617 2847 2219 Fragments arranged by size: 2847 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2191 2732 2827 3474 493 Size: 711 987 541 95 647 2702 Fragments arranged by size: 2702 987 711 647 541 95 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3490 3872 4507 275 Size: 53 83 186 936 1957 382 635 1451 Fragments arranged by size: 1957 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 4514 4788 4935 5346 4116 Size: 398 274 147 411 4453 Fragments arranged by size: 4453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2726 2756 1178 Size: 30 165 30 1323 30 4105 Fragments arranged by size: 4105 1323 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2453 1186 Size: 1267 4416 BseYI C'CCAG_C Cuts at: 4886 5382 4886 Size: 496 5187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2174 1163 Size: 573 438 4672 Fragments arranged by size: 4672 573 438 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3133 3894 4922 1540 Size: 275 186 1132 761 1028 2301 Fragments arranged by size: 2301 1132 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2094 2517 2958 3744 3829 4900 2094 Size: 423 441 786 85 1071 2877 Fragments arranged by size: 2877 1071 786 441 423 85 BsmI GAATG_Cn' Cuts at: 3194 3287 3194 Size: 93 5590 BsmBI CGTCTCn'nnnn_ Cuts at: 2096 2096 Size: 5683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2094 2517 2832 2958 3002 3744 Size: 399 252 52 423 315 126 44 742 Cuts at: 3744 3829 4900 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 423 399 315 252 126 85 52 44 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3038 844 Size: 2194 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3570 1394 Size: 429 1747 3507 Fragments arranged by size: 3507 1747 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2386 4178 4352 65 Size: 1183 1138 1792 174 1396 Fragments arranged by size: 1792 1396 1183 1138 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4327 4514 1700 Size: 110 2517 187 2869 Fragments arranged by size: 2869 2517 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5683 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 2191 2690 794 Size: 1397 499 3787 Fragments arranged by size: 3787 1397 499 BstBI TT'CG_AA Cuts at: 2938 4487 2938 Size: 1549 4134 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5683 BstYI r'GATC_y Cuts at: 1972 3013 3380 3765 3782 5234 5320 5332 5678 Size: 1041 367 385 17 1452 86 12 346 Cuts at: 5678 1972 Size: 1977 Fragments arranged by size: 1977 1452 1041 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2979 513 Size: 552 939 975 3217 Fragments arranged by size: 3217 975 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4944 Fragments arranged by size: 4944 683 56 BtsI GCAGTG_nn' Cuts at: 3199 3993 4013 3199 Size: 794 20 4869 Fragments arranged by size: 4869 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2584 3130 4019 Size: 54 1425 50 275 18 754 546 889 Cuts at: 4019 5648 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 754 546 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2862 3613 1353 Size: 1509 751 3423 Fragments arranged by size: 3423 1509 751 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4270 4644 4790 387 Size: 1064 138 2681 374 146 1280 Fragments arranged by size: 2681 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2515 2515 Size: 5683 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2465 3746 4343 5130 5398 1239 Size: 393 476 357 1281 597 787 268 1524 Fragments arranged by size: 1524 1281 787 597 476 393 357 268 EcoO109I rG'GnC_Cy Cuts at: 2081 2205 2081 Size: 124 5559 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2934 2934 Size: 5683 FspI TGC'GCA Cuts at: 4189 4189 Size: 5683 HaeII r_GCGC'y Cuts at: 1678 4830 1678 Size: 3152 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2726 2758 4324 Size: 32 135 32 530 32 592 32 1566 Cuts at: 4324 4356 4398 4430 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1566 592 530 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3027 3273 669 Size: 988 281 1089 246 3079 Fragments arranged by size: 3079 1089 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4797 4981 1305 Size: 3492 184 2007 Fragments arranged by size: 3492 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2586 10 Size: 54 1425 343 754 3107 Fragments arranged by size: 3107 1425 754 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3641 4000 4159 518 Size: 648 2475 359 159 2042 Fragments arranged by size: 2475 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3099 3776 4924 5169 1606 Size: 1493 677 1148 245 2120 Fragments arranged by size: 2120 1493 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2979 513 Size: 552 939 975 3217 Fragments arranged by size: 3217 975 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NsiI A_TGCA'T Cuts at: 2723 2887 2723 Size: 164 5519 NspI r_CATG'y Cuts at: 1132 1229 2725 2885 3041 4586 1132 Size: 97 1496 160 156 1545 2229 Fragments arranged by size: 2229 1545 1496 160 156 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5683 PciI A'CATG_T Cuts at: 4582 4582 Size: 5683 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5683 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PmlI CAC'GTG Cuts at: 2191 2732 2827 2191 Size: 541 95 5047 Fragments arranged by size: 5047 541 95 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2081 2205 2081 Size: 124 5559 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5683 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PstI C_TGCA'G Cuts at: 830 2143 3035 830 Size: 1313 892 3478 Fragments arranged by size: 3478 1313 892 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 SacI G_AGCT'C Cuts at: 2517 2517 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SfcI C'TryA_G Cuts at: 826 1045 2139 3031 4166 4847 5038 826 Size: 219 1094 892 1135 681 191 1471 Fragments arranged by size: 1471 1135 1094 892 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 3010 1872 Size: 1138 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2033 3787 4688 4950 820 Size: 197 926 90 1754 901 262 1553 Fragments arranged by size: 1754 1553 926 901 262 197 90 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 2968 3607 5 Size: 47 2916 639 2081 Fragments arranged by size: 2916 2081 639 47 StuI AGG'CCT Cuts at: 1517 2159 1517 Size: 642 5041 StyI C'CwwG_G Cuts at: 513 1065 2004 2642 2949 2979 3122 513 Size: 552 939 638 307 30 143 3074 Fragments arranged by size: 3074 939 638 552 307 143 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3710 3727 3880 4065 4510 325 Size: 1255 2130 17 153 185 445 1498 Fragments arranged by size: 2130 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3929 5641 96 Size: 274 80 33 51 1443 1952 1712 138 Fragments arranged by size: 1952 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5643 5675 5643 Size: 32 5651 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5683 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 3008 1870 Size: 1138 4545 XmnI GAAnn'nnTTC Cuts at: 2816 3812 2816 Size: 996 4687 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI