Restriction map for
pFN21AB7637
(Linear) (Six-base) MAPSORT of: KIBB7637.seq Check: 9349 from: 1 to: 978
KIBB7637.orf
With 182 enzymes: SgfI *
March 22, 2012 15:38 ..
AclI AA'CG_TT
Cuts at: 0 573 978
Size: 573 405
AhdI GACnn_n'nnGTC
Cuts at: 0 111 978
Size: 111 867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 159 163 978
Size: 159 4 815
Fragments arranged by size:
815 159 4
BanI G'GyrC_C
Cuts at: 0 401 978
Size: 401 577
BanII G_rGCy'C
Cuts at: 0 37 512 978
Size: 37 475 466
Fragments arranged by size:
475 466 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 905 978
Size: 905 73
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4 38 978
Size: 4 34 940
Fragments arranged by size:
940 34 4
BciVI GTATCCnnnnn_n'
Cuts at: 0 390 978
Size: 390 588
BfrBI ATG'CAT
Cuts at: 0 716 880 978
Size: 716 164 98
Fragments arranged by size:
716 164 98
Bme1580I G_kGCm'C
Cuts at: 0 827 978
Size: 827 151
BmrI ACTGGGnnnn_n'
Cuts at: 0 869 978
Size: 869 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 219 978
Size: 219 759
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 460 978
Size: 103 357 518
Fragments arranged by size:
518 357 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 978
Size: 13 965
BsaAI yAC'GTr
Cuts at: 0 186 727 822 978
Size: 186 541 95 156
Fragments arranged by size:
541 186 156 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 721 751 978
Size: 721 30 227
Fragments arranged by size:
721 227 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 448 978
Size: 448 530
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 978
Size: 169 809
BsiHKAI G_wGCw'C
Cuts at: 0 89 512 953 978
Size: 89 423 441 25
Fragments arranged by size:
441 423 89 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 91 978
Size: 91 887
Bsp1286I G_dGCh'C
Cuts at: 0 37 89 512 827 953 978
Size: 37 52 423 315 126 25
Fragments arranged by size:
423 315 126 52 37 25
BsrDI GCAATG_nn'
Cuts at: 0 381 978
Size: 381 597
BstAPI GCAn_nnn'nTGC
Cuts at: 0 186 685 978
Size: 186 499 293
Fragments arranged by size:
499 293 186
BstBI TT'CG_AA
Cuts at: 0 933 978
Size: 933 45
EaeI y'GGCC_r
Cuts at: 0 579 978
Size: 579 399
EarI CTCTTCn'nnn_
Cuts at: 0 857 978
Size: 857 121
EcoICRI GAG'CTC
Cuts at: 0 510 978
Size: 510 468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 460 978
Size: 103 357 518
Fragments arranged by size:
518 357 103
EcoO109I rG'GnC_Cy
Cuts at: 0 76 200 978
Size: 76 124 778
Fragments arranged by size:
778 124 76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 929 978
Size: 929 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 721 753 978
Size: 97 32 592 32 225
Fragments arranged by size:
592 225 97 32 32
MscI TGG'CCA
Cuts at: 0 581 978
Size: 581 397
NsiI A_TGCA'T
Cuts at: 0 718 882 978
Size: 718 164 96
Fragments arranged by size:
718 164 96
NspI r_CATG'y
Cuts at: 0 720 880 978
Size: 720 160 98
Fragments arranged by size:
720 160 98
PmlI CAC'GTG
Cuts at: 0 186 727 822 978
Size: 186 541 95 156
Fragments arranged by size:
541 186 156 95
PpuMI rG'GwC_Cy
Cuts at: 0 76 200 978
Size: 76 124 778
Fragments arranged by size:
778 124 76
PstI C_TGCA'G
Cuts at: 0 138 978
Size: 138 840
SacI G_AGCT'C
Cuts at: 0 512 978
Size: 512 466
SfcI C'TryA_G
Cuts at: 0 134 978
Size: 134 844
SmlI C'TyrA_G
Cuts at: 0 28 978
Size: 28 950
SspI AAT'ATT
Cuts at: 0 963 978
Size: 963 15
StuI AGG'CCT
Cuts at: 0 154 978
Size: 154 824
StyI C'CwwG_G
Cuts at: 0 637 944 978
Size: 637 307 34
Fragments arranged by size:
637 307 34
XmnI GAAnn'nnTTC
Cuts at: 0 811 978
Size: 811 167
Enzymes that do cut:
AclI AhdI AlfI BanI BanII BbsI BcgI BciVI
BfrBI Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaXI
BseRI BsgI BsiHKAI BsmBI Bsp1286I BsrDI BstAPI BstBI
EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I MscI
NsiI NspI PmlI PpuMI PstI SacI SfcI SmlI
SspI StuI StyI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BclI BglI BglII BlpI BmgBI BmtI Bpu10I BsaI
BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI