################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII Cfr10I CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MluI MroI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TspRI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcyI 3 GGCGTC G[GA]^CG[CT]C AflII 1102 CTTAAG C^TTAAG AspHI 1076 GAGCTC G[AT]GC[AT]^C BanII 1076 GAGCTC G[GA]GC[CT]^C BbvI 290 GCAGC GCAGC(8/12) BfaI 848 CTAG C^TAG BfrI 1102 CTTAAG C^TTAAG BglII 351 AGATCT A^GATCT BmyI 1076 GAGCTC G[AGT]GC[ACT]^C BpmI 16 CTGGAG CTGGAG(16/14) BsaHI 3 GGCGTC G[GA]^CG[CT]C BsaJI 272 CCCTGG C^C[ACGT][ACGT]GG BseRI 166 GAGGAG GAGGAG(10/8) BsgI 235 GTGCAG GTGCAG(16/14) BsiHKAI 1076 GAGCTC G[AT]GC[AT]^C BsmBI 5 CGTCTC CGTCTC(1/5) BsmFI 497 GTCCC GGGAC(10/14) Bsp1286I 1076 GAGCTC G[AGT]GC[ACT]^C BspDI 832 ATCGAT AT^CGAT BsrBI 19 GAGCGG CCGCTC(-3/-3) BsrI 1071 ACTGG ACTGG(1/-1) BssKI 273 CCTGG ^CC[ACGT]GG BstNI 273 CCTGG CC^[AT]GG Cac8I 1030 GCCAGC GC[ACGT]^[ACGT]GC CfoI 35 GCGC GCG^C ClaI 832 ATCGAT AT^CGAT EcoRI 401 GAATTC G^AATTC EcoRII 273 CCTGG ^CC[AT]GG HaeII 34 GGCGCT [GA]GCGC^[CT] HapII 73 CCGG C^CGG HgaI 4 GCGTC GACGC(5/10) HhaI 35 GCGC GCG^C Hin1I 3 GGCGTC G[GA]^CG[CT]C HinP1I 35 GCGC G^CGC HpaII 73 CCGG C^CGG HphI 853 GGTGA GGTGA(8/7) MaeI 848 CTAG C^TAG MfeI 983 CAATTG C^AATTG MspA1I 1085 CAGCTG C[AC]G^C[GT]G MspI 73 CCGG C^CGG MunI 983 CAATTG C^AATTG MvaI 273 CCTGG CC^[AT]GG NspI 421 GCATGT [GA]CATG^[CT] PvuII 1085 CAGCTG CAG^CTG SacI 1076 GAGCTC GAGCT^C ScrFI 273 CCTGG CC^[ACGT]GG SfaNI 1034 GCATC GCATC(5/9) SmlI 1102 CTTAAG C^T[CT][GA]AG SspI 634 AATATT AAT^ATT TseI 290 GCAGC G^C[AT]GC Tsp45I 425 GTGAC ^GT[GC]AC XcmI 152 CCAGAGGACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AciI 21 GCGG CCGC(-3/-1) 885 GCGG AcsI 49 GAATTT [GA]^AATT[CT] 401 GAATTC AfaI 305 GTAC GT^AC 397 GTAC AlwI 325 GATCC GGATC(4/5) 505 GGATC AocI 970 CCTAAGG CC^T[ACGT]AGG 994 CCTAAGG ApoI 49 GAATTT [GA]^AATT[CT] 401 GAATTC BalI 226 TGGCCA TGG^CCA 1028 TGGCCA BbsI 315 GAAGAC GAAGAC(2/6) 479 GAAGAC BpuAI 315 GAAGAC GAAGAC(2/6) 479 GAAGAC BsiYI 25 CCAGAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 583 CCTTTTAGTGG BslI 25 CCAGAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 583 CCTTTTAGTGG BsmAI 936 GAGAC GTCTC(1/5) 6 GTCTC BstF5I 640 CATCC GGATG(2/0) 411 GGATG BstYI 351 AGATCT [GA]^GATC[CT] 505 GGATCT Bsu36I 970 CCTAAGG CC^T[ACGT]AGG 994 CCTAAGG DdeI 971 CTAAG C^T[ACGT]AG 995 CTAAG Eco81I 970 CCTAAGG CC^T[ACGT]AGG 994 CCTAAGG FokI 640 CATCC GGATG(9/13) 411 GGATG HindIII 660 AAGCTT A^AGCTT 1001 AAGCTT HinfI 88 GAATC G^A[ACGT]TC 691 GATTC MflI 351 AGATCT [GA]^GATC[CT] 505 GGATCT MluNI 226 TGGCCA TGG^CCA 1028 TGGCCA MscI 226 TGGCCA TGG^CCA 1028 TGGCCA MslI 259 CATGTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 742 CACAAAAATG RsaI 305 GTAC GT^AC 397 GTAC TaqI 284 TCGA T^CGA 833 TCGA TfiI 88 GAATC G^A[AT]TC 691 GATTC TthHB8I 284 TCGA T^CGA 833 TCGA XhoII 351 AGATCT [GA]^GATC[CT] 505 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AluI AvaII Cfr13I DpnII EaeI Fnu4HI HaeIII ItaI MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I Tru9I Tsp509I