(Circular) (Six-base) MAPSORT of: pFN21AE5558.seq Check: 5740 from: 1 to: 5848 pFN21AE5558 5848 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3191 1937 Size: 1254 4594 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2396 3114 3911 5295 5563 1632 Size: 764 718 797 1384 268 1917 Fragments arranged by size: 1917 1384 797 764 718 268 AflII C'TTAA_G Cuts at: 820 1017 3107 820 Size: 197 2090 3561 Fragments arranged by size: 3561 2090 197 AflIII A'CryG_T Cuts at: 1203 4747 5724 1203 Size: 3544 977 1327 Fragments arranged by size: 3544 1327 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5848 AlwNI CAG_nnn'CTG Cuts at: 3591 5163 3591 Size: 1572 4276 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 2054 2406 3157 3383 3535 5644 1493 Size: 561 352 751 226 152 2109 1697 Fragments arranged by size: 2109 1697 751 561 352 226 152 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 3173 1870 Size: 73 1230 4545 Fragments arranged by size: 4545 1230 73 BamHI G'GATC_C Cuts at: 3178 3545 3178 Size: 367 5481 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3169 4624 618 Size: 325 217 627 1382 1455 1842 Fragments arranged by size: 1842 1455 1382 627 325 217 BanII G_rGCy'C Cuts at: 1391 3085 3167 1391 Size: 1694 82 4072 Fragments arranged by size: 4072 1694 82 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2327 2491 928 Size: 971 428 164 4285 Fragments arranged by size: 4285 971 428 164 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4039 4073 1903 Size: 34 2102 34 3678 Fragments arranged by size: 3678 2102 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3740 4950 3740 Size: 1210 4638 BclI T'GATC_A Cuts at: 1548 1548 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4459 136 Size: 107 122 71 631 605 2787 1525 Fragments arranged by size: 2787 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2356 5843 2356 Size: 3487 2361 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 3909 5065 1790 Size: 2119 1156 2573 Fragments arranged by size: 2573 2119 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5848 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3086 3579 4537 Size: 416 53 83 399 24 1669 493 958 Cuts at: 4537 442 Size: 1753 Fragments arranged by size: 1753 1669 958 493 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 4508 1239 Size: 803 2466 2579 Fragments arranged by size: 2579 2466 803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3973 4838 5136 3973 Size: 865 298 4685 Fragments arranged by size: 4685 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4511 882 Size: 617 3012 2219 Fragments arranged by size: 3012 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3639 493 Size: 711 2435 2702 Fragments arranged by size: 2702 2435 711 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3655 4037 4672 Size: 53 83 186 936 476 1646 382 635 Cuts at: 4672 275 Size: 1451 Fragments arranged by size: 1646 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4281 4679 4953 5100 5511 4281 Size: 398 274 147 411 4618 Fragments arranged by size: 4618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5623 Fragments arranged by size: 5623 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 1186 Size: 1000 4848 BseYI C'CCAG_C Cuts at: 5051 5547 5051 Size: 496 5352 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2261 1163 Size: 573 525 4750 Fragments arranged by size: 4750 573 525 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3298 4059 5087 1540 Size: 275 186 1297 761 1028 2301 Fragments arranged by size: 2301 1297 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3085 3909 3994 5065 3085 Size: 824 85 1071 3868 Fragments arranged by size: 3868 1071 824 85 BsmI GAATG_Cn' Cuts at: 3359 3452 3359 Size: 93 5755 BsmBI CGTCTCn'nnnn_ Cuts at: 2016 2016 Size: 5848 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3085 3167 3909 3994 5065 1391 Size: 399 1295 82 742 85 1071 2174 Fragments arranged by size: 2174 1295 1071 742 399 85 82 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3737 5467 3737 Size: 1730 4118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3203 844 Size: 2359 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2026 3735 1394 Size: 429 203 1709 3507 Fragments arranged by size: 3507 1709 429 203 BsrDI GCAATG_nn' Cuts at: 65 1248 4343 4517 65 Size: 1183 3095 174 1396 Fragments arranged by size: 3095 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4492 4679 1700 Size: 110 2682 187 2869 Fragments arranged by size: 2869 2682 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5848 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5848 BssSI C'ACGA_G Cuts at: 3908 4920 3908 Size: 1012 4836 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5848 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5848 BstYI r'GATC_y Cuts at: 1972 2356 2510 3178 3545 3930 3947 5399 5485 Size: 384 154 668 367 385 17 1452 86 Cuts at: 5485 5497 5843 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 668 385 384 367 346 154 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2976 3000 2976 Size: 24 5824 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4357 Fragments arranged by size: 4357 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 3364 4158 4178 3364 Size: 794 20 5034 Fragments arranged by size: 5034 794 20 ClaI AT'CG_AT Cuts at: 1554 2838 1554 Size: 1284 4564 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2027 2231 3033 Size: 54 1425 50 275 18 197 204 802 Cuts at: 3033 3295 4184 5813 8 Size: 262 889 1629 43 Fragments arranged by size: 1629 1425 889 802 275 262 204 197 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3295 1537 Size: 275 1483 4090 Fragments arranged by size: 4090 1483 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3778 1353 Size: 2425 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4435 4809 4955 387 Size: 1064 138 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3083 3083 Size: 5848 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2396 3114 3911 4508 5295 5563 Size: 393 410 354 718 797 597 787 268 Cuts at: 5563 1239 Size: 1524 Fragments arranged by size: 1524 797 787 718 597 410 393 354 268 EcoRI G'AATT_C Cuts at: 2406 3157 3535 2406 Size: 751 378 4719 Fragments arranged by size: 4719 751 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2639 2639 Size: 5848 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 2043 4995 1678 Size: 365 2952 2531 Fragments arranged by size: 2952 2531 365 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2314 2346 4489 4521 4563 Size: 32 135 32 742 32 2143 32 42 Cuts at: 4563 4595 1373 Size: 32 2626 Fragments arranged by size: 2626 2143 742 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3192 3438 669 Size: 988 281 1254 246 3079 Fragments arranged by size: 3079 1254 988 281 246 HindIII A'AGCT_T Cuts at: 748 2665 3006 748 Size: 1917 341 3590 Fragments arranged by size: 3590 1917 341 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 2988 3447 2988 Size: 459 5389 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2102 4962 5146 1305 Size: 797 2860 184 2007 Fragments arranged by size: 2860 2007 797 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2233 3035 10 Size: 54 1425 343 401 802 2823 Fragments arranged by size: 2823 1425 802 401 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2268 2751 3806 4165 4324 518 Size: 648 1102 483 1055 359 159 2042 Fragments arranged by size: 2042 1102 1055 648 483 359 159 MspA1I CmG'CkG Cuts at: 1606 3092 3264 3941 5089 5334 1606 Size: 1486 172 677 1148 245 2120 Fragments arranged by size: 2120 1486 1148 677 245 172 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4357 Fragments arranged by size: 4357 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 2430 3206 4751 1132 Size: 97 1201 776 1545 2229 Fragments arranged by size: 2229 1545 1201 776 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5848 PciI A'CATG_T Cuts at: 4747 4747 Size: 5848 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5848 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 2655 3418 2655 Size: 763 5085 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2385 2417 2385 Size: 32 5816 PstI C_TGCA'G Cuts at: 830 3200 830 Size: 2370 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 PvuII CAG'CTG Cuts at: 3092 3092 Size: 5848 SacI G_AGCT'C Cuts at: 3085 3085 Size: 5848 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 4096 5808 4096 Size: 1712 4136 SfcI C'TryA_G Cuts at: 826 1045 3196 4331 5012 5203 826 Size: 219 2151 1135 681 191 1471 Fragments arranged by size: 2151 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 3175 1872 Size: 1303 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3107 3952 4853 5115 820 Size: 197 926 1164 845 901 262 1553 Fragments arranged by size: 1553 1164 926 901 845 262 197 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 3206 1132 Size: 2074 3774 SspI AAT'ATT Cuts at: 5 52 2641 3772 5 Size: 47 2589 1131 2081 Fragments arranged by size: 2589 2081 1131 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 2004 3287 513 Size: 552 939 1283 3074 Fragments arranged by size: 3074 1283 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3875 3892 4045 4230 4675 325 Size: 1255 2295 17 153 185 445 1498 Fragments arranged by size: 2295 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2309 4094 5806 Size: 274 80 33 51 1443 332 1785 1712 Cuts at: 5806 96 Size: 138 Fragments arranged by size: 1785 1712 1443 332 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5808 5840 5808 Size: 32 5816 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5848 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2164 2164 Size: 5848 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 3173 1870 Size: 1303 4545 XmnI GAAnn'nnTTC Cuts at: 3977 3977 Size: 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI