################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BalI BamHI BbrPI BciVI BfrI BlnI BsaBI BsaWI BseAI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I FseI FspI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcyI 2119 GGCGCC G[GA]^CG[CT]C AflIII 381 ACACGT A^C[GA][CT]GT AhdI 1282 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 2672 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 489 CCTGAGG CC^T[ACGT]AGG Asp700I 1387 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1282 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 2119 GGCGCC GGCGC^C BcgI 248 CGAATCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1676 TGATCA T^GATCA BlpI 1191 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1191 GCTTAGC GC^T[ACGT]AGC BsaAI 382 CACGTA [CT]AC^GT[GA] BsaHI 2119 GGCGCC G[GA]^CG[CT]C BsaI 2261 GAGACC GGTCTC(1/5) BsgI 1615 GTGCAG GTGCAG(16/14) Bsp1407I 1760 TGTACA T^GTACA BspHI 2213 TCATGA T^CATGA BsrGI 1760 TGTACA T^GTACA BstAPI 402 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2604 GGTAACC G^GT[ACGT]ACC BstPI 2604 GGTAACC G^GT[ACGT]ACC Bsu36I 489 CCTGAGG CC^T[ACGT]AGG CelII 1191 GCTTAGC GC^T[ACGT]AGC CfoI 2120 GCGC GCG^C DraI 720 TTTAAA TTT^AAA DraII 1635 AGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 2084 TGGCCG [CT]^GGCC[GA] Eam1105I 1282 GACCTATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 489 CCTGAGG CC^T[ACGT]AGG EcoNI 1627 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1635 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 2604 GGTAACC G^GT[ACGT]ACC EcoRI 742 GAATTC G^AATTC EcoRV 1377 GATATC GAT^ATC EcoT22I 1247 ATGCAT ATGCA^T FauI 292 GCGGG CCCGC(4/6) FbaI 1676 TGATCA T^GATCA HaeII 2119 GGCGCC [GA]GCGC^[CT] HhaI 2120 GCGC GCG^C Hin1I 2119 GGCGCC G[GA]^CG[CT]C HinP1I 2120 GCGC G^CGC HpaI 519 GTTAAC GTT^AAC KasI 2119 GGCGCC G^GCGCC NarI 2119 GGCGCC GG^CGCC NsiI 1247 ATGCAT ATGCA^T PflMI 666 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuI 1201 CGATCG CGAT^CG RcaI 2213 TCATGA T^CATGA SapI 2313 GCTCTTC GCTCTTC(1/4) SfoI 2119 GGCGCC GGC^GCC SspBI 1760 TGTACA T^GTACA SwaI 719 ATTTAAAT ATTT^AAAT Van91I 666 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1387 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AfaI 385 GTAC GT^AC 1761 GTAC AlwI 1751 GATCC GGATC(4/5) 2487 GGATC AspHI 22 GTGCTC G[AT]GC[AT]^C 1010 GAGCAC AvaII 1281 GGACC G^G[AT]CC 1694 GGACC BbsI 1591 GAAGAC GAAGAC(2/6) 196 GTCTTC BbvCI 189 CCTCAGC CCTCAGC(-5/-2) 90 GCTGAGG BcnI 11 CCCGG CC^[GC]GG 59 CCGGG BglI 1831 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2065 GCCCTGCTGGC BpuAI 1591 GAAGAC GAAGAC(2/6) 196 GTCTTC BseRI 2361 CTCCTC GAGGAG(10/8) 277 GAGGAG BsiEI 56 CGACCG CG[GA][CT]^CG 1201 CGATCG BsiHKAI 22 GTGCTC G[AT]GC[AT]^C 1010 GAGCAC BspMI 1658 ACCTGC ACCTGC(4/8) 2636 ACCTGC DsaI 2081 CCATGG C^C[GA][CT]GG 2165 CCATGG EarI 1025 CTCTTC CTCTTC(1/4) 2314 CTCTTC HapII 12 CCGG C^CGG 59 CCGG HgaI 194 GCGTC GACGC(5/10) 429 GCGTC HincII 519 GTTAAC GT[CT]^[GA]AC 1171 GTTGAC HindII 519 GTTAAC GT[CT]^[GA]AC 1171 GTTGAC HindIII 1123 AAGCTT A^AGCTT 2541 AAGCTT HpaII 12 CCGG C^CGG 59 CCGG Ksp632I 1025 CTCTTC CTCTTC(1/4) 2314 CTCTTC MslI 859 CATTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1240 CACCCCAATG MspA1I 49 CAGCTG C[AC]G^C[GT]G 238 CAGCTG MspI 12 CCGG C^CGG 59 CCGG NciI 11 CCCGG CC^[GC]GG 59 CCGGG NcoI 2081 CCATGG C^CATGG 2165 CCATGG NspI 1808 ACATGC [GA]CATG^[CT] 2437 GCATGT PvuII 49 CAGCTG CAG^CTG 238 CAGCTG RsaI 385 GTAC GT^AC 1761 GTAC SspI 2000 AATATT AAT^ATT 2272 AATATT TaqI 811 TCGA T^CGA 2570 TCGA TthHB8I 811 TCGA T^CGA 2570 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AluI ApoI BanI BanII BbvI BfaI BglII BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII