(Circular) (Six-base) MAPSORT of: pFN21AE9441.seq Check: 7378 from: 1 to: 7426 pFN21AE9441 7426 bp With 182 enzymes: SgfI * February 2, 2012 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3672 3672 Size: 7426 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5236 278 Size: 53 83 186 936 3700 2468 Fragments arranged by size: 3700 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4769 1937 Size: 2832 4594 Acc65I G'GTAC_C Cuts at: 4747 4747 Size: 7426 AclI AA'CG_TT Cuts at: 1563 5553 5926 1563 Size: 3990 373 3063 Fragments arranged by size: 3990 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3116 3930 4419 5489 6873 7141 1632 Size: 1484 814 489 1070 1384 268 1917 Fragments arranged by size: 1917 1484 1384 1070 814 489 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7229 AflIII A'CryG_T Cuts at: 1203 2386 6325 7302 1203 Size: 1183 3939 977 1327 Fragments arranged by size: 3939 1327 1183 977 AgeI A'CCGG_T Cuts at: 6257 6257 Size: 7426 AhdI GACnn_n'nnGTC Cuts at: 3292 6155 3292 Size: 2863 4563 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7426 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2159 1184 Size: 975 6451 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3977 4009 3977 Size: 32 7394 AlwNI CAG_nnn'CTG Cuts at: 4682 5169 6741 4682 Size: 487 1572 5367 Fragments arranged by size: 5367 1572 487 ApaLI G'TGCA_C Cuts at: 5483 6639 5483 Size: 1156 6270 ApoI r'AATT_y Cuts at: 1493 2410 2747 2963 3214 4016 4735 4961 5113 Size: 917 337 216 251 802 719 226 152 Cuts at: 5113 7222 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 917 802 719 337 251 226 216 152 AseI AT'TA_AT Cuts at: 160 1032 5980 160 Size: 872 4948 1606 Fragments arranged by size: 4948 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7426 AvaI C'yCGr_G Cuts at: 1870 1943 4751 1870 Size: 73 2808 4545 Fragments arranged by size: 4545 2808 73 BamHI G'GATC_C Cuts at: 4756 5123 4756 Size: 367 7059 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2499 2945 3887 4124 4747 Size: 325 217 627 712 446 942 237 623 Cuts at: 4747 6202 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 942 712 627 623 446 325 237 217 BanII G_rGCy'C Cuts at: 1391 2130 2175 2703 4164 4745 1391 Size: 739 45 528 1461 581 4072 Fragments arranged by size: 4072 1461 739 581 528 45 BbeI G_GCGC'C Cuts at: 4128 4128 Size: 7426 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2194 3603 928 Size: 971 295 1409 4751 Fragments arranged by size: 4751 1409 971 295 BbvCI CC'TCA_GC Cuts at: 2096 2195 2096 Size: 99 7327 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2242 2276 5617 5651 1903 Size: 34 305 34 3341 34 3678 Fragments arranged by size: 3678 3341 305 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5318 6528 5318 Size: 1210 6216 BclI T'GATC_A Cuts at: 1548 3681 1548 Size: 2133 5293 BfrBI ATG'CAT Cuts at: 3254 3254 Size: 7426 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3842 4076 6037 Size: 107 122 71 631 605 2170 234 1961 Cuts at: 6037 136 Size: 1525 Fragments arranged by size: 2170 1961 1525 631 605 234 122 107 71 BglII A'GATC_T Cuts at: 2897 3021 3076 7421 2897 Size: 124 55 4345 2902 Fragments arranged by size: 4345 2902 124 55 BlpI GC'TnA_GC Cuts at: 3197 4843 3197 Size: 1646 5780 Bme1580I G_kGCm'C Cuts at: 1790 2466 4072 5487 6643 1790 Size: 676 1606 1415 1156 2573 Fragments arranged by size: 2573 1606 1415 1156 676 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7426 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2259 3102 3333 Size: 416 53 83 399 24 842 843 231 Cuts at: 3333 4644 5157 6115 442 Size: 1311 513 958 1753 Fragments arranged by size: 1753 1311 958 843 842 513 416 399 231 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7426 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2617 3014 3033 3814 4004 4292 4609 Size: 1061 317 397 19 781 190 288 317 Cuts at: 4609 6086 1239 Size: 1477 2579 Fragments arranged by size: 2579 1477 1061 781 397 317 317 288 190 19 Bpu10I CC'TnA_GC Cuts at: 2096 2147 2195 2096 Size: 51 48 7327 Fragments arranged by size: 7327 51 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3392 3537 4584 5551 6416 6714 3392 Size: 145 1047 967 865 298 4104 Fragments arranged by size: 4104 1047 967 865 298 145 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4260 6089 882 Size: 617 2761 1829 2219 Fragments arranged by size: 2761 2219 1829 617 BsaAI yAC'GTr Cuts at: 493 1204 2389 5217 493 Size: 711 1185 2828 2702 Fragments arranged by size: 2828 2702 1185 711 BsaBI GATnn'nnATC Cuts at: 7420 7420 Size: 7426 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4125 5233 5615 6250 Size: 53 83 186 936 2592 1108 382 635 Cuts at: 6250 275 Size: 1451 Fragments arranged by size: 2592 1451 1108 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5859 6257 6531 6678 7089 5859 Size: 398 274 147 411 6196 Fragments arranged by size: 6196 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3048 3078 3977 4007 4015 Size: 30 165 30 1645 30 899 30 8 Cuts at: 4015 4045 1178 Size: 30 4559 Fragments arranged by size: 4559 1645 899 165 30 30 30 30 30 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 4357 1186 Size: 1111 2060 4255 Fragments arranged by size: 4255 2060 1111 BseYI C'CCAG_C Cuts at: 4105 4163 6629 7125 4105 Size: 58 2466 496 4406 Fragments arranged by size: 4406 2466 496 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3641 1163 Size: 573 1905 4948 Fragments arranged by size: 4948 1905 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2064 3209 4876 5637 6665 1540 Size: 275 186 63 1145 1667 761 1028 2301 Fragments arranged by size: 2301 1667 1145 1028 761 275 186 63 BsiHKAI G_wGCw'C Cuts at: 2031 3019 5487 5572 6643 2031 Size: 988 2468 85 1071 2814 Fragments arranged by size: 2814 2468 1071 988 85 BsmI GAATG_Cn' Cuts at: 2330 2488 3469 4937 5030 2330 Size: 158 981 1468 93 4726 Fragments arranged by size: 4726 1468 981 158 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2130 2175 2466 2703 3019 4072 Size: 399 241 99 45 291 237 316 1053 Cuts at: 4072 4164 4745 5487 5572 6643 1391 Size: 92 581 742 85 1071 2174 Fragments arranged by size: 2174 1071 1053 742 581 399 316 291 241 237 99 92 85 45 BspEI T'CCGG_A Cuts at: 7089 7089 Size: 7426 BspHI T'CATG_A Cuts at: 4218 5315 7045 4218 Size: 1097 1730 4599 Fragments arranged by size: 4599 1730 1097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3672 4650 4781 844 Size: 2828 978 131 3489 Fragments arranged by size: 3489 2828 978 131 BsrBI CCG'CTC Cuts at: 1394 1823 5313 1394 Size: 429 3490 3507 Fragments arranged by size: 3507 3490 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2119 2564 4227 4305 5921 6095 65 Size: 1183 871 445 1663 78 1616 174 1396 Fragments arranged by size: 1663 1616 1396 1183 871 445 174 78 BsrFI r'CCGG_y Cuts at: 1700 1810 6070 6257 1700 Size: 110 4260 187 2869 Fragments arranged by size: 4260 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3765 96 Size: 3669 3757 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7426 BssSI C'ACGA_G Cuts at: 5486 6498 5486 Size: 1012 6414 BstAPI GCAn_nnn'nTGC Cuts at: 794 2413 794 Size: 1619 5807 BstBI TT'CG_AA Cuts at: 6230 6230 Size: 7426 BstEII G'GTnAC_C Cuts at: 1184 4609 1184 Size: 3425 4001 BstXI CCAn_nnnn'nTGG Cuts at: 2581 2891 3923 4171 2581 Size: 310 1032 248 5836 Fragments arranged by size: 5836 1032 310 248 BstYI r'GATC_y Cuts at: 1972 2897 3021 3076 3755 4492 4756 5123 5508 Size: 925 124 55 679 737 264 367 385 Cuts at: 5508 5525 6977 7063 7075 7421 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 925 737 679 385 367 346 264 124 86 55 17 12 Bsu36I CC'TnA_GG Cuts at: 2495 2495 Size: 7426 BtgI C'CryG_G Cuts at: 513 1065 2004 4086 4170 513 Size: 552 939 2082 84 3769 Fragments arranged by size: 3769 2082 939 552 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3784 487 Size: 683 56 2558 4129 Fragments arranged by size: 4129 2558 683 56 BtsI GCAGTG_nn' Cuts at: 2754 3629 3813 4942 5736 5756 2754 Size: 875 184 1129 794 20 4424 Fragments arranged by size: 4424 1129 875 794 184 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7426 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7391 DraI TTT'AAA Cuts at: 2727 4730 2727 Size: 2003 5423 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6433 809 Size: 507 5117 1802 Fragments arranged by size: 5117 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4089 4873 5762 Size: 54 1425 50 275 18 2259 784 889 Cuts at: 5762 7391 8 Size: 1629 43 Fragments arranged by size: 2259 1629 1425 889 784 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4873 1537 Size: 275 3061 4090 Fragments arranged by size: 4090 3061 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3036 4325 5356 1353 Size: 1683 1289 1031 3423 Fragments arranged by size: 3423 1683 1289 1031 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6013 6387 6533 387 Size: 1064 138 4424 374 146 1280 Fragments arranged by size: 4424 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2617 3014 3033 3116 3814 3930 Size: 393 668 317 397 19 83 698 116 Cuts at: 3930 4004 4292 4419 4609 5489 6086 6873 7141 Size: 74 288 127 190 880 597 787 268 Cuts at: 7141 1239 Size: 1524 Fragments arranged by size: 1524 880 787 698 668 597 397 393 317 288 268 190 127 116 83 74 19 EcoNI CCTnn'n_nnAGG Cuts at: 3636 3636 Size: 7426 EcoO109I rG'GnC_Cy Cuts at: 3641 3641 Size: 7426 EcoRI G'AATT_C Cuts at: 2747 4735 5113 2747 Size: 1988 378 5060 Fragments arranged by size: 5060 1988 378 EcoRV GAT'ATC Cuts at: 3384 3384 Size: 7426 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2707 2707 Size: 7426 FspI TGC'GCA Cuts at: 5932 5932 Size: 7426 HaeII r_GCGC'y Cuts at: 1678 4128 6573 1678 Size: 2450 2445 2531 Fragments arranged by size: 2531 2450 2445 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3006 3013 3038 3045 3278 Size: 32 135 32 1434 7 25 7 233 Cuts at: 3278 3310 3466 3498 4678 4710 6067 6099 6141 Size: 32 156 32 1180 32 1357 32 42 Cuts at: 6141 6173 1373 Size: 32 2626 Fragments arranged by size: 2626 1434 1357 1180 233 156 135 42 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2526 3178 4770 5016 669 Size: 988 281 588 652 1592 246 3079 Fragments arranged by size: 3079 1592 988 652 588 281 246 HindIII A'AGCT_T Cuts at: 748 3128 4546 748 Size: 2380 1418 3628 Fragments arranged by size: 3628 2380 1418 HpaI GTT'AAC Cuts at: 2526 5016 2526 Size: 2490 4936 KasI G'GCGC_C Cuts at: 4124 4124 Size: 7426 KpnI G_GTAC'C Cuts at: 4751 4751 Size: 7426 MfeI C'AATT_G Cuts at: 5025 5025 Size: 7426 MluI A'CGCG_T Cuts at: 7302 7302 Size: 7426 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2187 2214 2799 3321 6540 6724 1305 Size: 882 27 585 522 3219 184 2007 Fragments arranged by size: 3219 2007 882 585 522 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5604 Fragments arranged by size: 5604 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2868 3249 5384 5743 5902 518 Size: 648 1702 381 2135 359 159 2042 Fragments arranged by size: 2135 2042 1702 648 381 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2245 4842 5519 6667 6912 1606 Size: 450 189 2597 677 1148 245 2120 Fragments arranged by size: 2597 2120 1148 677 450 245 189 NarI GG'CG_CC Cuts at: 4125 4125 Size: 7426 NcoI C'CATG_G Cuts at: 513 1065 2004 4086 4170 513 Size: 552 939 2082 84 3769 Fragments arranged by size: 3769 2082 939 552 84 NdeI CA'TA_TG Cuts at: 387 387 Size: 7426 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7426 NotI GC'GGCC_GC Cuts at: 4873 4873 Size: 7426 NsiI A_TGCA'T Cuts at: 3256 3256 Size: 7426 NspI r_CATG'y Cuts at: 1132 1229 3817 4446 4784 6329 1132 Size: 97 2588 629 338 1545 2229 Fragments arranged by size: 2588 2229 1545 629 338 97 PasI CC'CwG_GG Cuts at: 1348 2677 1348 Size: 1329 6097 PciI A'CATG_T Cuts at: 6325 6325 Size: 7426 PflMI CCAn_nnn'nTGG Cuts at: 1108 2677 1108 Size: 1569 5857 PfoI T'CCnGG_A Cuts at: 2277 2277 Size: 7426 PmeI GTTT'AAAC Cuts at: 4730 4730 Size: 7426 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2920 2952 3977 4009 5495 5527 7008 7040 2920 Size: 32 1025 32 1486 32 1481 32 3306 Fragments arranged by size: 3306 1486 1481 1025 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7426 PsiI TTA'TAA Cuts at: 4996 4996 Size: 7426 PstI C_TGCA'G Cuts at: 830 2055 2370 2409 4778 830 Size: 1225 315 39 2369 3478 Fragments arranged by size: 3478 2369 1225 315 39 PvuI CG_AT'CG Cuts at: 2001 3209 2001 Size: 1208 6218 PvuII CAG'CTG Cuts at: 2056 2245 2056 Size: 189 7237 SalI G'TCGA_C Cuts at: 1936 4768 1936 Size: 2832 4594 SapI GCTCTTCn'nnn_ Cuts at: 4325 4325 Size: 7426 SbfI CC_TGCA'GG Cuts at: 4778 4778 Size: 7426 ScaI AGT'ACT Cuts at: 5674 7386 5674 Size: 1712 5714 SfcI C'TryA_G Cuts at: 826 1045 2051 2366 2405 4774 5909 6590 6781 Size: 219 1006 315 39 2369 1135 681 191 Cuts at: 6781 826 Size: 1471 Fragments arranged by size: 2369 1471 1135 1006 681 315 219 191 39 SfoI GGC'GCC Cuts at: 4126 4126 Size: 7426 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7426 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7426 SmaI CCC'GGG Cuts at: 1872 4753 1872 Size: 2881 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3407 3552 4599 5530 6431 6693 Size: 197 926 1464 145 1047 931 901 262 Cuts at: 6693 820 Size: 1553 Fragments arranged by size: 1553 1464 1047 931 926 901 262 197 145 SnaBI TAC'GTA Cuts at: 493 5217 493 Size: 4724 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7426 SphI G_CATG'C Cuts at: 1132 4784 1132 Size: 3652 3774 SspI AAT'ATT Cuts at: 5 52 4007 4279 5350 5 Size: 47 3955 272 1071 2081 Fragments arranged by size: 3955 2081 1071 272 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7426 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 2580 4086 4170 4605 4865 Size: 552 939 318 258 1506 84 435 260 Cuts at: 4865 513 Size: 3074 Fragments arranged by size: 3074 1506 939 552 435 318 260 258 84 SwaI ATTT'AAAT Cuts at: 2727 2727 Size: 7426 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4097 4557 5453 5470 5623 5808 6253 Size: 1255 2517 460 896 17 153 185 445 Cuts at: 6253 325 Size: 1498 Fragments arranged by size: 2517 1498 1255 896 460 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3765 5672 7384 Size: 274 80 33 51 1443 1788 1907 1712 Cuts at: 7384 96 Size: 138 Fragments arranged by size: 1907 1788 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3481 3513 7386 7418 3481 Size: 32 3873 32 3489 Fragments arranged by size: 3873 3489 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7426 XbaI T'CTAG_A Cuts at: 4762 4762 Size: 7426 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3709 3838 4177 3709 Size: 129 339 6958 Fragments arranged by size: 6958 339 129 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7426 XmaI C'CCGG_G Cuts at: 1870 4751 1870 Size: 2881 4545 XmnI GAAnn'nnTTC Cuts at: 3396 5555 3396 Size: 2159 5267 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5234 276 Size: 53 83 186 936 3700 2468 Fragments arranged by size: 3700 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI