(Linear) (Six-base) MAPSORT of: KIEE9441.seq Check: 9733 from: 1 to: 2721 KIEE9441.orf With 182 enzymes: SgfI * February 2, 2012 14:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1667 2721 Size: 1667 1054 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1111 1925 2414 2721 Size: 1111 814 489 307 Fragments arranged by size: 1111 814 489 307 AflIII A'CryG_T Cuts at: 0 381 2721 Size: 381 2340 AhdI GACnn_n'nnGTC Cuts at: 0 1287 2721 Size: 1287 1434 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 154 2721 Size: 154 2567 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1972 2004 2721 Size: 1972 32 717 Fragments arranged by size: 1972 717 32 AlwNI CAG_nnn'CTG Cuts at: 0 2677 2721 Size: 2677 44 ApoI r'AATT_y Cuts at: 0 405 742 958 1209 2011 2721 Size: 405 337 216 251 802 710 Fragments arranged by size: 802 710 405 337 251 216 BanI G'GyrC_C Cuts at: 0 494 940 1882 2119 2721 Size: 494 446 942 237 602 Fragments arranged by size: 942 602 494 446 237 BanII G_rGCy'C Cuts at: 0 125 170 698 2159 2721 Size: 125 45 528 1461 562 Fragments arranged by size: 1461 562 528 125 45 BbeI G_GCGC'C Cuts at: 0 2123 2721 Size: 2123 598 BbsI GAAGACnn'nnnn_ Cuts at: 0 189 1598 2721 Size: 189 1409 1123 Fragments arranged by size: 1409 1123 189 BbvCI CC'TCA_GC Cuts at: 0 91 190 2721 Size: 91 99 2531 Fragments arranged by size: 2531 99 91 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 237 271 2721 Size: 237 34 2450 Fragments arranged by size: 2450 237 34 BclI T'GATC_A Cuts at: 0 1676 2721 Size: 1676 1045 BfrBI ATG'CAT Cuts at: 0 1249 2721 Size: 1249 1472 BglI GCCn_nnn'nGGC Cuts at: 0 1837 2071 2721 Size: 1837 234 650 Fragments arranged by size: 1837 650 234 BglII A'GATC_T Cuts at: 0 892 1016 1071 2721 Size: 892 124 55 1650 Fragments arranged by size: 1650 892 124 55 BlpI GC'TnA_GC Cuts at: 0 1192 2721 Size: 1192 1529 Bme1580I G_kGCm'C Cuts at: 0 461 2067 2721 Size: 461 1606 654 Fragments arranged by size: 1606 654 461 BmrI ACTGGGnnnn_n' Cuts at: 0 254 1097 1328 2639 2721 Size: 254 843 231 1311 82 Fragments arranged by size: 1311 843 254 231 82 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 612 1009 1028 1809 1999 2287 2604 Size: 295 317 397 19 781 190 288 317 Cuts at: 2604 2721 Size: 117 Fragments arranged by size: 781 397 317 317 295 288 190 117 19 Bpu10I CC'TnA_GC Cuts at: 0 91 142 190 2721 Size: 91 51 48 2531 Fragments arranged by size: 2531 91 51 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1387 1532 2579 2721 Size: 1387 145 1047 142 Fragments arranged by size: 1387 1047 145 142 BsaI GGTCTCn'nnnn_ Cuts at: 0 2255 2721 Size: 2255 466 BsaAI yAC'GTr Cuts at: 0 384 2721 Size: 384 2337 BsaHI Gr'CG_yC Cuts at: 0 2120 2721 Size: 2120 601 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1043 1073 1972 2002 2010 2040 2721 Size: 1043 30 899 30 8 30 681 Fragments arranged by size: 1043 899 681 30 30 30 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 292 2352 2721 Size: 292 2060 369 Fragments arranged by size: 2060 369 292 BseYI C'CCAG_C Cuts at: 0 2100 2158 2721 Size: 2100 58 563 Fragments arranged by size: 2100 563 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1636 2721 Size: 1636 1085 BsiEI CG_ry'CG Cuts at: 0 59 1204 2721 Size: 59 1145 1517 Fragments arranged by size: 1517 1145 59 BsiHKAI G_wGCw'C Cuts at: 0 26 1014 2721 Size: 26 988 1707 Fragments arranged by size: 1707 988 26 BsmI GAATG_Cn' Cuts at: 0 325 483 1464 2721 Size: 325 158 981 1257 Fragments arranged by size: 1257 981 325 158 Bsp1286I G_dGCh'C Cuts at: 0 26 125 170 461 698 1014 2067 2159 Size: 26 99 45 291 237 316 1053 92 Cuts at: 2159 2721 Size: 562 Fragments arranged by size: 1053 562 316 291 237 99 92 45 26 BspHI T'CATG_A Cuts at: 0 2213 2721 Size: 2213 508 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1667 2645 2721 Size: 1667 978 76 Fragments arranged by size: 1667 978 76 BsrDI GCAATG_nn' Cuts at: 0 114 559 2222 2300 2721 Size: 114 445 1663 78 421 Fragments arranged by size: 1663 445 421 114 78 BsrGI T'GTAC_A Cuts at: 0 1760 2721 Size: 1760 961 BstAPI GCAn_nnn'nTGC Cuts at: 0 408 2721 Size: 408 2313 BstEII G'GTnAC_C Cuts at: 0 2604 2721 Size: 2604 117 BstXI CCAn_nnnn'nTGG Cuts at: 0 576 886 1918 2166 2721 Size: 576 310 1032 248 555 Fragments arranged by size: 1032 576 555 310 248 BstYI r'GATC_y Cuts at: 0 892 1016 1071 1750 2487 2721 Size: 892 124 55 679 737 234 Fragments arranged by size: 892 737 679 234 124 55 Bsu36I CC'TnA_GG Cuts at: 0 490 2721 Size: 490 2231 BtgI C'CryG_G Cuts at: 0 2081 2165 2721 Size: 2081 84 556 Fragments arranged by size: 2081 556 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1779 2721 Size: 1779 942 BtsI GCAGTG_nn' Cuts at: 0 749 1624 1808 2721 Size: 749 875 184 913 Fragments arranged by size: 913 875 749 184 DraI TTT'AAA Cuts at: 0 722 2721 Size: 722 1999 EaeI y'GGCC_r Cuts at: 0 2084 2721 Size: 2084 637 EarI CTCTTCn'nnn_ Cuts at: 0 1031 2320 2721 Size: 1031 1289 401 Fragments arranged by size: 1289 1031 401 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 295 612 1009 1028 1111 1809 1925 1999 Size: 295 317 397 19 83 698 116 74 Cuts at: 1999 2287 2414 2604 2721 Size: 288 127 190 117 Fragments arranged by size: 698 397 317 295 288 190 127 117 116 83 74 19 EcoNI CCTnn'n_nnAGG Cuts at: 0 1631 2721 Size: 1631 1090 EcoO109I rG'GnC_Cy Cuts at: 0 1636 2721 Size: 1636 1085 EcoRI G'AATT_C Cuts at: 0 742 2721 Size: 742 1979 EcoRV GAT'ATC Cuts at: 0 1379 2721 Size: 1379 1342 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 702 2721 Size: 702 2019 HaeII r_GCGC'y Cuts at: 0 2123 2721 Size: 2123 598 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1001 1008 1033 1040 1273 1305 1461 1493 Size: 1001 7 25 7 233 32 156 32 Cuts at: 1493 2673 2705 2721 Size: 1180 32 16 Fragments arranged by size: 1180 1001 233 156 32 32 32 25 16 7 7 HincII GTy'rAC Cuts at: 0 521 1173 2721 Size: 521 652 1548 Fragments arranged by size: 1548 652 521 HindIII A'AGCT_T Cuts at: 0 1123 2541 2721 Size: 1123 1418 180 Fragments arranged by size: 1418 1123 180 HpaI GTT'AAC Cuts at: 0 521 2721 Size: 521 2200 KasI G'GCGC_C Cuts at: 0 2119 2721 Size: 2119 602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 182 209 794 1316 2721 Size: 182 27 585 522 1405 Fragments arranged by size: 1405 585 522 182 27 MslI CAynn'nnrTG Cuts at: 0 863 1244 2721 Size: 863 381 1477 Fragments arranged by size: 1477 863 381 MspA1I CmG'CkG Cuts at: 0 51 240 2721 Size: 51 189 2481 Fragments arranged by size: 2481 189 51 NarI GG'CG_CC Cuts at: 0 2120 2721 Size: 2120 601 NcoI C'CATG_G Cuts at: 0 2081 2165 2721 Size: 2081 84 556 Fragments arranged by size: 2081 556 84 NsiI A_TGCA'T Cuts at: 0 1251 2721 Size: 1251 1470 NspI r_CATG'y Cuts at: 0 1812 2441 2721 Size: 1812 629 280 Fragments arranged by size: 1812 629 280 PasI CC'CwG_GG Cuts at: 0 672 2721 Size: 672 2049 PflMI CCAn_nnn'nTGG Cuts at: 0 672 2721 Size: 672 2049 PfoI T'CCnGG_A Cuts at: 0 272 2721 Size: 272 2449 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 915 947 1972 2004 2721 Size: 915 32 1025 32 717 Fragments arranged by size: 1025 915 717 32 32 PstI C_TGCA'G Cuts at: 0 50 365 404 2721 Size: 50 315 39 2317 Fragments arranged by size: 2317 315 50 39 PvuI CG_AT'CG Cuts at: 0 1204 2721 Size: 1204 1517 PvuII CAG'CTG Cuts at: 0 51 240 2721 Size: 51 189 2481 Fragments arranged by size: 2481 189 51 SapI GCTCTTCn'nnn_ Cuts at: 0 2320 2721 Size: 2320 401 SfcI C'TryA_G Cuts at: 0 46 361 400 2721 Size: 46 315 39 2321 Fragments arranged by size: 2321 315 46 39 SfoI GGC'GCC Cuts at: 0 2121 2721 Size: 2121 600 SmlI C'TyrA_G Cuts at: 0 1402 1547 2594 2721 Size: 1402 145 1047 127 Fragments arranged by size: 1402 1047 145 127 SspI AAT'ATT Cuts at: 0 2002 2274 2721 Size: 2002 272 447 Fragments arranged by size: 2002 447 272 StyI C'CwwG_G Cuts at: 0 317 575 2081 2165 2600 2721 Size: 317 258 1506 84 435 121 Fragments arranged by size: 1506 435 317 258 121 84 SwaI ATTT'AAAT Cuts at: 0 722 2721 Size: 722 1999 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2092 2552 2721 Size: 2092 460 169 Fragments arranged by size: 2092 460 169 TatI w'GTAC_w Cuts at: 0 1760 2721 Size: 1760 961 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1476 1508 2721 Size: 1476 32 1213 Fragments arranged by size: 1476 1213 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1704 1833 2172 2721 Size: 1704 129 339 549 Fragments arranged by size: 1704 549 339 129 XmnI GAAnn'nnTTC Cuts at: 0 1391 2721 Size: 1391 1330 Enzymes that do cut: AarI AcuI AflIII AhdI AlfI AloI AlwNI ApoI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI MmeI MslI MspA1I NarI NcoI NsiI NspI PasI PflMI PfoI PpiI PstI PvuI PvuII SapI SfcI SfoI SmlI SspI StyI SwaI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AleI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BciVI BmgBI BmtI BplI BsaBI BsaWI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstBI BstZ17I ClaI CspCI DraIII DrdI EagI EciI EcoICRI FseI FspI FspAI KpnI MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI Tth111I XbaI XhoI XmaI ZraI