################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeIII HapII HgaI Hin1I HincII HindII HpaI HpaII HphI KasI KpnI KspI MaeIII MamI MfeI MflI MluI MluNI MnlI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspBI StuI SwaI TaqI Tsp45I TspRI Tth111I TthHB8I Van91I XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 612 AAATTT [GA]^AATT[CT] AfaI 97 GTAC GT^AC ApoI 612 AAATTT [GA]^AATT[CT] AvaII 684 GGTCC G^G[AT]CC AvrII 312 CCTAGG C^CTAGG BanI 187 GGTGCC G^G[CT][GA]CC BciVI 308 GTATCC GTATCC(6/5) BlnI 312 CCTAGG C^CTAGG BsaJI 312 CCTAGG C^C[ACGT][ACGT]GG BsiYI 291 CCTGCAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 291 CCTGCAGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BspHI 197 TCATGA T^CATGA BsrDI 107 GCAATG GCAATG(2/0) CfoI 386 GCGC GCG^C Cfr13I 684 GGTCC G^G[ACGT]CC DdeI 332 CTAAG C^T[ACGT]AG DraI 445 TTTAAA TTT^AAA EarI 439 GAAGAG CTCTTC(1/4) EcoT14I 312 CCTAGG C^C[AT][AT]GG HaeII 385 AGCGCC [GA]GCGC^[CT] HhaI 386 GCGC GCG^C HinP1I 386 GCGC G^CGC HindIII 376 AAGCTT A^AGCTT Ksp632I 439 GAAGAG CTCTTC(1/4) MaeII 158 ACGT A^CGT MseI 446 TTAA T^TAA MwoI 181 GCTGTAGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 187 GGTGCC GG[ACGT]^[ACGT]CC PleI 112 GACTC GAGTC(4/5) PstI 292 CTGCAG CTGCA^G RcaI 197 TCATGA T^CATGA RsaI 97 GTAC GT^AC Sau96I 684 GGTCC G^G[ACGT]CC SbfI 291 CCTGCAGG CCTGCA^GG SmlI 116 CTCAAG C^T[CT][GA]AG Sse8387I 291 CCTGCAGG CCTGCA^GG SspI 26 AATATT AAT^ATT StyI 312 CCTAGG C^C[AT][AT]GG TfiI 201 GATTC G^A[AT]TC Tru9I 446 TTAA T^TAA XbaI 522 TCTAGA T^CTAGA XcmI 543 CCAAAGCTATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 224 GCGG CCGC(-3/-1) 455 GCGG AlwI 75 GATCC GGATC(4/5) 282 GATCC BsaI 534 GAGACC GGTCTC(1/5) 574 GAGACC BsmAI 534 GAGAC GTCTC(1/5) 574 GAGAC BspMI 4 GCAGGT ACCTGC(4/8) 294 GCAGGT BsrI 645 ACTGG ACTGG(1/-1) 663 ACTGG BssKI 213 CCTGG ^CC[ACGT]GG 414 CCAGG BstNI 213 CCTGG CC^[AT]GG 414 CCAGG EcoRII 213 CCTGG ^CC[AT]GG 414 CCAGG HinfI 112 GACTC G^A[ACGT]TC 201 GATTC MboII 136 GAAGA GAAGA(8/7) 439 GAAGA MslI 192 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 423 CATAATTGTG MvaI 213 CCTGG CC^[AT]GG 414 CCAGG NspI 217 GCATGC [GA]CATG^[CT] 248 GCATGC ScrFI 213 CCTGG CC^[ACGT]GG 414 CCAGG SfcI 182 CTGTAG C^T[GA][CT]AG 292 CTGCAG SphI 217 GCATGC GCATG^C 248 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI BbvI BfaI Cac8I DpnII Fnu4HI ItaI MaeI MboI NdeII NlaIII Sau3AI TseI Tsp509I