Restriction map for
pFN21AE1655
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# NO CUT ENZYMES ( 202 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Cac8I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DpnII
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HapII
HhaI Hin1I HinP1I HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MboI MflI MluI MluNI
MroI MscI MslI MspA1I MspI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI Sau96I
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AciI 177 CCGC CCGC(-3/-1)
AcsI 9 GAATTT [GA]^AATT[CT]
AlwNI 250 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 339 CCTTAGG CC^T[ACGT]AGG
ApoI 9 GAATTT [GA]^AATT[CT]
Asp700I 247 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BfaI 383 CTAG C^TAG
BsaJI 100 CCAAGG C^C[ACGT][ACGT]GG
BsiYI 398 CCATTACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 398 CCATTACCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 48 GTCTC GTCTC(1/5)
BsmFI 114 GTCCC GGGAC(10/14)
BsrDI 57 CATTGC GCAATG(2/0)
BsrI 465 ACTGG ACTGG(1/-1)
BstF5I 407 GGATG GGATG(2/0)
Bsu36I 339 CCTTAGG CC^T[ACGT]AGG
DdeI 340 CTTAG C^T[ACGT]AG
Eco81I 339 CCTTAGG CC^T[ACGT]AGG
EcoT14I 100 CCAAGG C^C[AT][AT]GG
FokI 407 GGATG GGATG(9/13)
HgaI 127 GACGC GACGC(5/10)
MaeI 383 CTAG C^TAG
MaeII 275 ACGT A^CGT
MfeI 292 CAATTG C^AATTG
MunI 292 CAATTG C^AATTG
SspI 417 AATATT AAT^ATT
StyI 100 CCAAGG C^C[AT][AT]GG
Tsp45I 40 GTCAC ^GT[GC]AC
XcmI 455 CCATCAGTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 247 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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BbsI 172 GAAGAC GAAGAC(2/6)
271 GAAGAC
BbvI 145 GCAGC GCAGC(8/12)
302 GCTGC
BpuAI 172 GAAGAC GAAGAC(2/6)
271 GAAGAC
BssKI 404 CCAGG ^CC[ACGT]GG
482 CCTGG
BstNI 404 CCAGG CC^[AT]GG
482 CCTGG
EcoRII 404 CCAGG ^CC[AT]GG
482 CCTGG
Fnu4HI 145 GCAGC GC^[ACGT]GC
302 GCTGC
HincII 124 GTTGAC GT[CT]^[GA]AC
284 GTCAAC
HindII 124 GTTGAC GT[CT]^[GA]AC
284 GTCAAC
HinfI 165 GATTC G^A[ACGT]TC
443 GAATC
HphI 6 GGTGA GGTGA(8/7)
83 TCACC
ItaI 145 GCAGC GC^[ACGT]GC
302 GCTGC
MaeIII 40 GTCAC ^GT[ACGT]AC
95 GTTAC
MnlI 21 GAGG CCTC(7/6)
325 GAGG
MseI 199 TTAA T^TAA
488 TTAA
MvaI 404 CCAGG CC^[AT]GG
482 CCTGG
MwoI 121 GCTGTTGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
373 GCTTCATCAGC
NlaIII 149 CATG CATG^
267 CATG
ScrFI 404 CCAGG CC^[ACGT]GG
482 CCTGG
TfiI 165 GATTC G^A[AT]TC
443 GAATC
Tru9I 199 TTAA T^TAA
488 TTAA
TseI 145 GCAGC G^C[AT]GC
302 GCTGC
TspRI 358 CACTG CA[GC]TG[ACGT][ACGT]^
459 CAGTG
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
AluI MboII Tsp509I