################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccIII AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI CelII ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PstI PvuI RcaI RsrII SalI SbfI SexAI SfaNI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AclI 847 AACGTT AA^CGTT AflII 1659 CTTAAG C^TTAAG AlwNI 695 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1485 CCTGAGG CC^T[ACGT]AGG ApaI 17 GGGCCC GGGCC^C AseI 810 ATTAAT AT^TAAT AsnI 810 ATTAAT AT^TAAT AspHI 79 GAGCTC G[AT]GC[AT]^C AspI 281 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC BclI 906 TGATCA T^GATCA BfrI 1659 CTTAAG C^TTAAG BsaWI 950 TCCGGT [AT]^CCGG[AT] BseRI 1400 CTCCTC GAGGAG(10/8) BsgI 841 CTGCAC GTGCAG(16/14) BsiHKAI 79 GAGCTC G[AT]GC[AT]^C BsrFI 1268 GCCGGT [GA]^CCGG[CT] Bst1107I 535 GTATAC GTA^TAC BstAPI 39 GCACACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 797 TTCGAA TT^CGAA BstEII 250 GGTGACC G^GT[ACGT]ACC BstPI 250 GGTGACC G^GT[ACGT]ACC BstXI 781 CCACTCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 535 GTATAC GTA^TAC Bsu36I 1485 CCTGAGG CC^T[ACGT]AGG Cfr10I 1268 GCCGGT [GA]^CCGG[CT] Eco81I 1485 CCTGAGG CC^T[ACGT]AGG EcoNI 255 CCTTTTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 250 GGTGACC G^GT[ACGT]ACC FbaI 906 TGATCA T^GATCA HindIII 1589 AAGCTT A^AGCTT KspI 21 CCGCGG CCGC^GG MaeII 848 ACGT A^CGT MslI 981 CATAAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 938 CCATGG C^CATGG NdeI 563 CATATG CA^TATG NspV 797 TTCGAA TT^CGAA PflFI 281 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 270 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 263 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 810 ATTAAT AT^TAAT Psp1406I 847 AACGTT AA^CGTT SacI 79 GAGCTC GAGCT^C SacII 21 CCGCGG CCGC^GG SapI 1251 GCTCTTC GCTCTTC(1/4) SfcI 1071 CTACAG C^T[GA][CT]AG SfuI 797 TTCGAA TT^CGAA Tth111I 281 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 270 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1206 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BanII 17 GGGCCC G[GA]GC[CT]^C 79 GAGCTC BsmFI 279 GGGAC GGGAC(10/14) 954 GTCCC BsmI 1522 GAATGC GAATGC(1/-1) 1707 GCATTC BspMI 56 ACCTGC ACCTGC(4/8) 839 ACCTGC BssHII 26 GCGCGC G^CGCGC 110 GCGCGC DraI 580 TTTAAA TTT^AAA 1317 TTTAAA HapII 951 CCGG C^CGG 1269 CCGG HpaII 951 CCGG C^CGG 1269 CCGG MspI 951 CCGG C^CGG 1269 CCGG NspI 1019 ACATGC [GA]CATG^[CT] 1598 ACATGC PleI 1503 GACTC GAGTC(4/5) 327 GAGTC ScaI 438 AGTACT AGT^ACT 1333 AGTACT SmlI 1337 CTCAAG C^T[CT][GA]AG 1659 CTTAAG StuI 1182 AGGCCT AGG^CCT 1482 AGGCCT XcmI 189 CCAAGGTAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 389 CCAATGTCACCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI ApoI AvaII BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII