(Circular) (Six-base) MAPSORT of: pFN21AB6149.seq Check: 9144 from: 1 to: 6493 pFN21AB6149 6493 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4303 278 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2190 2541 3075 3836 1937 Size: 253 351 534 761 4594 Fragments arranged by size: 4594 761 534 351 253 Acc65I G'GTAC_C Cuts at: 3814 3814 Size: 6493 AclI AA'CG_TT Cuts at: 1563 2853 4620 4993 1563 Size: 1290 1767 373 3063 Fragments arranged by size: 3063 1767 1290 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2322 3376 3384 3681 4556 5940 6208 1632 Size: 690 1054 8 297 875 1384 268 1917 Fragments arranged by size: 1917 1384 1054 875 690 297 268 8 AflII C'TTAA_G Cuts at: 820 1017 3664 820 Size: 197 2647 3649 Fragments arranged by size: 3649 2647 197 AflIII A'CryG_T Cuts at: 1203 5392 6369 1203 Size: 4189 977 1327 Fragments arranged by size: 4189 1327 977 AgeI A'CCGG_T Cuts at: 5324 5324 Size: 6493 AhdI GACnn_n'nnGTC Cuts at: 5222 5222 Size: 6493 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6493 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6493 AlwNI CAG_nnn'CTG Cuts at: 2705 4236 5808 2705 Size: 1531 1572 3390 Fragments arranged by size: 3390 1572 1531 ApaI G_GGCC'C Cuts at: 2026 2026 Size: 6493 ApaLI G'TGCA_C Cuts at: 4550 5706 4550 Size: 1156 5337 ApoI r'AATT_y Cuts at: 1493 2494 3241 3755 3802 4028 4180 6289 1493 Size: 1001 747 514 47 226 152 2109 1697 Fragments arranged by size: 2109 1697 1001 747 514 226 152 47 AseI AT'TA_AT Cuts at: 160 1032 2816 5047 160 Size: 872 1784 2231 1606 Fragments arranged by size: 2231 1784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 AvaI C'yCGr_G Cuts at: 1870 1943 3818 1870 Size: 73 1875 4545 Fragments arranged by size: 4545 1875 73 BamHI G'GATC_C Cuts at: 3823 4190 3823 Size: 367 6126 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3814 5269 618 Size: 325 217 627 2027 1455 1842 Fragments arranged by size: 2027 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2026 2088 3812 1391 Size: 635 62 1724 4072 Fragments arranged by size: 4072 1724 635 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5522 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4684 4718 1903 Size: 34 2747 34 3678 Fragments arranged by size: 3678 2747 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4385 5595 4385 Size: 1210 5283 BclI T'GATC_A Cuts at: 1548 2911 1548 Size: 1363 5130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5104 136 Size: 107 122 71 631 605 3432 1525 Fragments arranged by size: 3432 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6488 6488 Size: 6493 BlpI GC'TnA_GC Cuts at: 3910 3910 Size: 6493 Bme1580I G_kGCm'C Cuts at: 1790 2026 2236 4554 5710 1790 Size: 236 210 2318 1156 2573 Fragments arranged by size: 2573 2318 1156 236 210 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6493 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3603 4224 5182 Size: 416 53 83 399 24 2186 621 958 Cuts at: 5182 442 Size: 1753 Fragments arranged by size: 2186 1753 958 621 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6493 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2258 3437 3690 5153 1239 Size: 1019 1179 253 1463 2579 Fragments arranged by size: 2579 1463 1179 1019 253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3327 4618 5483 5781 3327 Size: 1291 865 298 4039 Fragments arranged by size: 4039 1291 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5156 882 Size: 617 3657 2219 Fragments arranged by size: 3657 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4284 493 Size: 711 3080 2702 Fragments arranged by size: 3080 2702 711 BsaBI GATnn'nnATC Cuts at: 6487 6487 Size: 6493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4300 4682 5317 275 Size: 53 83 186 936 2767 382 635 1451 Fragments arranged by size: 2767 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2955 4926 5324 5598 5745 6156 2955 Size: 1971 398 274 147 411 3292 Fragments arranged by size: 3292 1971 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2256 2286 1178 Size: 30 165 30 853 30 5385 Fragments arranged by size: 5385 853 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3396 1186 Size: 2210 4283 BseYI C'CCAG_C Cuts at: 2119 5696 6192 2119 Size: 3577 496 2420 Fragments arranged by size: 3577 2420 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2831 1163 Size: 573 1095 4825 Fragments arranged by size: 4825 1095 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3943 4704 5732 1540 Size: 275 186 1942 761 1028 2301 Fragments arranged by size: 2301 1942 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2088 4554 4639 5710 2088 Size: 2466 85 1071 2871 Fragments arranged by size: 2871 2466 1071 85 BsmI GAATG_Cn' Cuts at: 3533 3712 4004 4097 3533 Size: 179 292 93 5929 Fragments arranged by size: 5929 292 179 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2088 2236 3812 4554 4639 5710 Size: 399 236 62 148 1576 742 85 1071 Cuts at: 5710 1391 Size: 2174 Fragments arranged by size: 2174 1576 1071 742 399 236 148 85 62 BspEI T'CCGG_A Cuts at: 6156 6156 Size: 6493 BspHI T'CATG_A Cuts at: 4382 6112 4382 Size: 1730 4763 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2070 2853 3848 844 Size: 1226 783 995 3489 Fragments arranged by size: 3489 1226 995 783 BsrBI CCG'CTC Cuts at: 1394 1823 4380 1394 Size: 429 2557 3507 Fragments arranged by size: 3507 2557 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4988 5162 65 Size: 1183 3740 174 1396 Fragments arranged by size: 3740 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3273 5137 5324 1700 Size: 110 1463 1864 187 2869 Fragments arranged by size: 2869 1864 1463 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6493 BssHII G'CGCG_C Cuts at: 1925 2031 2115 1925 Size: 106 84 6303 Fragments arranged by size: 6303 106 84 BssSI C'ACGA_G Cuts at: 4553 5565 4553 Size: 1012 5481 BstAPI GCAn_nnn'nTGC Cuts at: 794 2050 794 Size: 1256 5237 BstBI TT'CG_AA Cuts at: 2803 5297 2803 Size: 2494 3999 BstEII G'GTnAC_C Cuts at: 1184 2255 1184 Size: 1071 5422 BstXI CCAn_nnnn'nTGG Cuts at: 2793 2793 Size: 6493 BstYI r'GATC_y Cuts at: 1972 3157 3367 3688 3823 4190 4575 4592 6044 Size: 1185 210 321 135 367 385 17 1452 Cuts at: 6044 6130 6142 6488 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1185 385 367 346 321 210 135 86 17 12 BstZ17I GTA'TAC Cuts at: 2542 2542 Size: 6493 Bsu36I CC'TnA_GG Cuts at: 3491 3491 Size: 6493 BtgI C'CryG_G Cuts at: 513 1065 2004 2026 2403 2943 513 Size: 552 939 22 377 540 4063 Fragments arranged by size: 4063 939 552 540 377 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5754 Fragments arranged by size: 5754 683 56 BtsI GCAGTG_nn' Cuts at: 2331 2612 2771 4009 4803 4823 2331 Size: 281 159 1238 794 20 4001 Fragments arranged by size: 4001 1238 794 281 159 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6458 DraI TTT'AAA Cuts at: 2587 3324 3797 2587 Size: 737 473 5283 Fragments arranged by size: 5283 737 473 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5500 809 Size: 507 4184 1802 Fragments arranged by size: 4184 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3940 4829 6458 Size: 54 1425 50 275 18 2110 889 1629 Cuts at: 6458 8 Size: 43 Fragments arranged by size: 2110 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3940 1537 Size: 275 2128 4090 Fragments arranged by size: 4090 2128 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2339 2618 3027 3176 3263 4423 1353 Size: 986 279 409 149 87 1160 3423 Fragments arranged by size: 3423 1160 986 409 279 149 87 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5080 5454 5600 387 Size: 1064 138 3491 374 146 1280 Fragments arranged by size: 3491 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2086 2086 Size: 6493 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2322 3376 3384 3437 3681 3690 Size: 393 626 64 1054 8 53 244 9 Cuts at: 3690 4556 5153 5940 6208 1239 Size: 866 597 787 268 1524 Fragments arranged by size: 1524 1054 866 787 626 597 393 268 244 64 53 9 8 EcoNI CCTnn'n_nnAGG Cuts at: 2264 2264 Size: 6493 EcoO109I rG'GnC_Cy Cuts at: 2022 2269 3669 2022 Size: 247 1400 4846 Fragments arranged by size: 4846 1400 247 EcoRI G'AATT_C Cuts at: 3802 4180 3802 Size: 378 6115 FspI TGC'GCA Cuts at: 4999 4999 Size: 6493 HaeII r_GCGC'y Cuts at: 1678 5640 1678 Size: 3962 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2272 2304 5134 5166 5208 Size: 32 135 32 700 32 2830 32 42 Cuts at: 5208 5240 1373 Size: 32 2626 Fragments arranged by size: 2830 2626 700 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3837 4083 669 Size: 988 281 1899 246 3079 Fragments arranged by size: 3079 1899 988 281 246 HindIII A'AGCT_T Cuts at: 748 3594 748 Size: 2846 3647 HpaI GTT'AAC Cuts at: 4083 4083 Size: 6493 KpnI G_GTAC'C Cuts at: 3818 3818 Size: 6493 MfeI C'AATT_G Cuts at: 4092 4092 Size: 6493 MluI A'CGCG_T Cuts at: 6369 6369 Size: 6493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5607 5791 1305 Size: 4302 184 2007 Fragments arranged by size: 4302 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4671 Fragments arranged by size: 4671 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2990 4451 4810 4969 518 Size: 648 1824 1461 359 159 2042 Fragments arranged by size: 2042 1824 1461 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2028 2106 2157 3151 3744 3909 4586 5734 Size: 422 78 51 994 593 165 677 1148 Cuts at: 5734 5979 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 994 677 593 422 245 165 78 51 NcoI C'CATG_G Cuts at: 513 1065 2004 2943 513 Size: 552 939 939 4063 Fragments arranged by size: 4063 939 939 552 NdeI CA'TA_TG Cuts at: 387 2569 387 Size: 2182 4311 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6493 NotI GC'GGCC_GC Cuts at: 3940 3940 Size: 6493 NspI r_CATG'y Cuts at: 1132 1229 3028 3607 3851 5396 1132 Size: 97 1799 579 244 1545 2229 Fragments arranged by size: 2229 1799 1545 579 244 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6493 PciI A'CATG_T Cuts at: 5392 5392 Size: 6493 PflMI CCAn_nnn'nTGG Cuts at: 1108 2281 1108 Size: 1173 5320 PfoI T'CCnGG_A Cuts at: 2274 3414 2274 Size: 1140 5353 PmeI GTTT'AAAC Cuts at: 3797 3797 Size: 6493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3730 3762 4562 4594 6075 6107 3730 Size: 32 800 32 1481 32 4116 Fragments arranged by size: 4116 1481 800 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2269 2269 Size: 6493 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6493 PsiI TTA'TAA Cuts at: 4063 4063 Size: 6493 PspOMI G'GGCC_C Cuts at: 2022 2022 Size: 6493 PstI C_TGCA'G Cuts at: 830 3845 830 Size: 3015 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6493 PvuII CAG'CTG Cuts at: 2106 2157 3151 3744 2106 Size: 51 994 593 4855 Fragments arranged by size: 4855 994 593 51 SacI G_AGCT'C Cuts at: 2088 2088 Size: 6493 SacII CC_GC'GG Cuts at: 2029 2029 Size: 6493 SalI G'TCGA_C Cuts at: 1936 3835 1936 Size: 1899 4594 SapI GCTCTTCn'nnn_ Cuts at: 3263 3263 Size: 6493 SbfI CC_TGCA'GG Cuts at: 3845 3845 Size: 6493 ScaI AGT'ACT Cuts at: 2445 3340 4741 6453 2445 Size: 895 1401 1712 2485 Fragments arranged by size: 2485 1712 1401 895 SfcI C'TryA_G Cuts at: 826 1045 3076 3841 4976 5657 5848 826 Size: 219 2031 765 1135 681 191 1471 Fragments arranged by size: 2031 1471 1135 765 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6493 SmaI CCC'GGG Cuts at: 1872 3820 1872 Size: 1948 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3342 3664 4597 5498 5760 820 Size: 197 926 1399 322 933 901 262 1553 Fragments arranged by size: 1553 1399 933 926 901 322 262 197 SnaBI TAC'GTA Cuts at: 493 4284 493 Size: 3791 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6493 SphI G_CATG'C Cuts at: 1132 3851 1132 Size: 2719 3774 SspI AAT'ATT Cuts at: 5 52 4417 5 Size: 47 4365 2081 Fragments arranged by size: 4365 2081 47 StuI AGG'CCT Cuts at: 1517 3189 3489 1517 Size: 1672 300 4521 Fragments arranged by size: 4521 1672 300 StyI C'CwwG_G Cuts at: 513 1065 2004 2194 2792 2943 3932 513 Size: 552 939 190 598 151 989 3074 Fragments arranged by size: 3074 989 939 598 552 190 151 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2682 2857 4520 4537 4690 4875 5320 Size: 1255 1102 175 1663 17 153 185 445 Cuts at: 5320 325 Size: 1498 Fragments arranged by size: 1663 1498 1255 1102 445 185 175 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2443 3036 3338 Size: 274 80 33 51 1443 466 593 302 Cuts at: 3338 3780 4739 6451 96 Size: 442 959 1712 138 Fragments arranged by size: 1712 1443 959 593 466 442 302 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2942 2974 6453 6485 2942 Size: 32 3479 32 2950 Fragments arranged by size: 3479 2950 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2289 1316 Size: 973 5520 XbaI T'CTAG_A Cuts at: 3211 3829 3211 Size: 618 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2201 2401 2201 Size: 200 6293 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6493 XmaI C'CCGG_G Cuts at: 1870 3818 1870 Size: 1948 4545 XmnI GAAnn'nnTTC Cuts at: 4622 4622 Size: 6493 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4301 276 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI