################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BciVI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboII MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 153 GTAC GT^AC AlwI 217 GATCC GGATC(4/5) AlwNI 121 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 71 CCTCAGG CC^T[ACGT]AGG AvaII 176 GGACC G^G[AT]CC BanI 496 GGCACC G^G[CT][GA]CC BanII 550 GAGCTC G[GA]GC[CT]^C BcgI 512 GCAGTGGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 314 TGATCA T^GATCA BcnI 528 CCCGG CC^[GC]GG BglI 523 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 283 TCCGGT [AT]^CCGG[AT] BsiYI 528 CCCGGCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 528 CCCGGCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 175 GGGAC GGGAC(10/14) BsmI 7 GCATTC GAATGC(1/-1) BstF5I 359 GGATG GGATG(2/0) BstYI 216 AGATCC [GA]^GATC[CT] Bsu36I 71 CCTCAGG CC^T[ACGT]AGG Eco81I 71 CCTCAGG CC^T[ACGT]AGG EcoT14I 402 CCTTGG C^C[AT][AT]GG FbaI 314 TGATCA T^GATCA FokI 359 GGATG GGATG(9/13) HinfI 280 GACTC G^A[ACGT]TC MflI 216 AGATCC [GA]^GATC[CT] MseI 260 TTAA T^TAA NciI 528 CCCGG CC^[GC]GG NlaIII 157 CATG CATG^ PleI 280 GACTC GAGTC(4/5) PstI 148 CTGCAG CTGCA^G RsaI 153 GTAC GT^AC SacI 550 GAGCTC GAGCT^C SfaNI 360 GATGC GCATC(5/9) SfcI 148 CTGCAG C^T[GA][CT]AG StyI 402 CCTTGG C^C[AT][AT]GG TaqI 54 TCGA T^CGA Tru9I 260 TTAA T^TAA TthHB8I 54 TCGA T^CGA XhoII 216 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 274 AAATTT [GA]^AATT[CT] 456 AAATTC AluI 379 AGCT AG^CT 551 AGCT ApoI 274 AAATTT [GA]^AATT[CT] 456 AAATTC AspHI 395 GTGCTC G[AT]GC[AT]^C 550 GAGCTC BsiHKAI 395 GTGCTC G[AT]GC[AT]^C 550 GAGCTC BspMI 14 ACCTGC ACCTGC(4/8) 480 ACCTGC BsrI 436 ACTGG ACTGG(1/-1) 43 CCAGT BstNI 220 CCTGG CC^[AT]GG 534 CCAGG Cac8I 38 GCCAGC GC[ACGT]^[ACGT]GC 523 GCCAGC DpnII 217 GATC ^GATC 315 GATC DraIII 307 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG 364 CACAGGGTG EcoRII 220 CCTGG ^CC[AT]GG 534 CCAGG HapII 284 CCGG C^CGG 529 CCGG HpaII 284 CCGG C^CGG 529 CCGG HphI 27 GGTGA GGTGA(8/7) 399 TCACC MaeIII 28 GTGAC ^GT[ACGT]AC 160 GTCAC MboI 217 GATC ^GATC 315 GATC MspI 284 CCGG C^CGG 529 CCGG MvaI 220 CCTGG CC^[AT]GG 534 CCAGG NdeII 217 GATC ^GATC 315 GATC NlaIV 175 GGGACC GG[ACGT]^[ACGT]CC 496 GGCACC Sau3AI 217 GATC ^GATC 315 GATC Tsp45I 28 GTGAC ^GT[GC]AC 160 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AciI BbvI BmyI BsaJI Bsp1286I BssKI Cfr13I DdeI Fnu4HI HaeIII ItaI MnlI MwoI Sau96I ScrFI TseI Tsp509I TspRI