(Circular) (Six-base) MAPSORT of: pFN21AE6516.seq Check: 2189 from: 1 to: 5260 pFN21AE6516 5260 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2494 2494 Size: 5260 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3070 278 Size: 53 83 186 936 1534 2468 Fragments arranged by size: 2468 1534 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2603 1937 Size: 666 4594 Acc65I G'GTAC_C Cuts at: 2581 2581 Size: 5260 AclI AA'CG_TT Cuts at: 1563 3387 3760 1563 Size: 1824 373 3063 Fragments arranged by size: 3063 1824 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3323 4707 4975 1632 Size: 1691 1384 268 1917 Fragments arranged by size: 1917 1691 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5063 AflIII A'CryG_T Cuts at: 1203 4159 5136 1203 Size: 2956 977 1327 Fragments arranged by size: 2956 1327 977 AgeI A'CCGG_T Cuts at: 4091 4091 Size: 5260 AhdI GACnn_n'nnGTC Cuts at: 3989 3989 Size: 5260 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5260 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5260 AlwNI CAG_nnn'CTG Cuts at: 2131 3003 4575 2131 Size: 872 1572 2816 Fragments arranged by size: 2816 1572 872 ApaLI G'TGCA_C Cuts at: 3317 4473 3317 Size: 1156 4104 ApoI r'AATT_y Cuts at: 1493 2279 2461 2569 2795 2947 5056 1493 Size: 786 182 108 226 152 2109 1697 Fragments arranged by size: 2109 1697 786 226 182 152 108 AseI AT'TA_AT Cuts at: 160 1032 3814 160 Size: 872 2782 1606 Fragments arranged by size: 2782 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5260 AvaI C'yCGr_G Cuts at: 1870 1943 2585 1870 Size: 73 642 4545 Fragments arranged by size: 4545 642 73 BamHI G'GATC_C Cuts at: 2590 2957 2590 Size: 367 4893 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2501 2581 4036 618 Size: 325 217 627 714 80 1455 1842 Fragments arranged by size: 1842 1455 714 627 325 217 80 BanII G_rGCy'C Cuts at: 1391 2559 2579 1391 Size: 1168 20 4072 Fragments arranged by size: 4072 1168 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4289 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2506 2540 3451 3485 1903 Size: 34 569 34 911 34 3678 Fragments arranged by size: 3678 911 569 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3152 4362 3152 Size: 1210 4050 BclI T'GATC_A Cuts at: 1548 2319 1548 Size: 771 4489 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2534 3871 136 Size: 107 122 71 631 605 862 1337 1525 Fragments arranged by size: 1525 1337 862 631 605 122 107 71 BglII A'GATC_T Cuts at: 5255 5255 Size: 5260 BlpI GC'TnA_GC Cuts at: 2677 2677 Size: 5260 Bme1580I G_kGCm'C Cuts at: 1790 2415 3321 4477 1790 Size: 625 906 1156 2573 Fragments arranged by size: 2573 1156 906 625 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5260 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2451 2991 3949 Size: 416 53 83 399 24 1034 540 958 Cuts at: 3949 442 Size: 1753 Fragments arranged by size: 1753 1034 958 540 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5260 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3920 1239 Size: 2681 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3385 4250 4548 3385 Size: 865 298 4097 Fragments arranged by size: 4097 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3923 882 Size: 617 2424 2219 Fragments arranged by size: 2424 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3051 493 Size: 711 1847 2702 Fragments arranged by size: 2702 1847 711 BsaBI GATnn'nnATC Cuts at: 5254 5254 Size: 5260 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3067 3449 4084 275 Size: 53 83 186 936 1534 382 635 1451 Fragments arranged by size: 1534 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2288 3693 4091 4365 4512 4923 2288 Size: 1405 398 274 147 411 2625 Fragments arranged by size: 2625 1405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5035 Fragments arranged by size: 5035 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5260 BseYI C'CCAG_C Cuts at: 2121 4463 4959 2121 Size: 2342 496 2422 Fragments arranged by size: 2422 2342 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4687 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2710 3471 4499 1540 Size: 275 186 709 761 1028 2301 Fragments arranged by size: 2301 1028 761 709 275 186 BsiHKAI G_wGCw'C Cuts at: 2404 2559 3321 3406 4477 2404 Size: 155 762 85 1071 3187 Fragments arranged by size: 3187 1071 762 155 85 BsmI GAATG_Cn' Cuts at: 2012 2771 2864 2012 Size: 759 93 4408 Fragments arranged by size: 4408 759 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2404 2415 2559 2579 3321 3406 4477 Size: 399 614 11 144 20 742 85 1071 Cuts at: 4477 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 614 399 144 85 20 11 BspEI T'CCGG_A Cuts at: 4923 4923 Size: 5260 BspHI T'CATG_A Cuts at: 3149 4879 3149 Size: 1730 3530 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2028 2494 2615 844 Size: 1184 466 121 3489 Fragments arranged by size: 3489 1184 466 121 BsrBI CCG'CTC Cuts at: 1394 1823 3147 1394 Size: 429 1324 3507 Fragments arranged by size: 3507 1324 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3755 3929 65 Size: 1183 2507 174 1396 Fragments arranged by size: 2507 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3904 4091 1700 Size: 110 2094 187 2869 Fragments arranged by size: 2869 2094 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5260 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5260 BssSI C'ACGA_G Cuts at: 3320 4332 3320 Size: 1012 4248 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5260 BstBI TT'CG_AA Cuts at: 4064 4064 Size: 5260 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5260 BstYI r'GATC_y Cuts at: 1972 2221 2590 2957 3342 3359 4811 4897 4909 Size: 249 369 367 385 17 1452 86 12 Cuts at: 4909 5255 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 369 367 346 249 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2077 2077 Size: 5260 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4708 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4521 Fragments arranged by size: 4521 683 56 BtsI GCAGTG_nn' Cuts at: 2503 2524 2776 3570 3590 2503 Size: 21 252 794 20 4173 Fragments arranged by size: 4173 794 252 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5260 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5225 DraI TTT'AAA Cuts at: 2564 2564 Size: 5260 DraIII CAC_nnn'GTG Cuts at: 2317 2374 2317 Size: 57 5203 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4267 809 Size: 507 2951 1802 Fragments arranged by size: 2951 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2707 3596 5225 Size: 54 1425 50 275 18 877 889 1629 Cuts at: 5225 8 Size: 43 Fragments arranged by size: 1629 1425 889 877 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2707 1537 Size: 275 895 4090 Fragments arranged by size: 4090 895 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3190 1353 Size: 1837 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3847 4221 4367 387 Size: 1064 138 2258 374 146 1280 Fragments arranged by size: 2258 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2557 2557 Size: 5260 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3323 3920 4707 4975 1239 Size: 393 1691 597 787 268 1524 Fragments arranged by size: 1691 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2569 2947 2569 Size: 378 4882 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2131 2131 Size: 5260 FspI TGC'GCA Cuts at: 3766 3766 Size: 5260 HaeII r_GCGC'y Cuts at: 1678 4407 1678 Size: 2729 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3901 3933 3975 4007 1373 Size: 32 135 32 2329 32 42 32 2626 Fragments arranged by size: 2626 2329 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2604 2850 669 Size: 988 281 666 246 3079 Fragments arranged by size: 3079 988 666 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5260 HpaI GTT'AAC Cuts at: 2850 2850 Size: 5260 KpnI G_GTAC'C Cuts at: 2585 2585 Size: 5260 MfeI C'AATT_G Cuts at: 2859 2859 Size: 5260 MluI A'CGCG_T Cuts at: 5136 5136 Size: 5260 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2462 4374 4558 1305 Size: 1157 1912 184 2007 Fragments arranged by size: 2007 1912 1157 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3438 Fragments arranged by size: 3438 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3218 3577 3736 518 Size: 648 2052 359 159 2042 Fragments arranged by size: 2052 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2676 3353 4501 4746 1606 Size: 1070 677 1148 245 2120 Fragments arranged by size: 2120 1148 1070 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4708 NdeI CA'TA_TG Cuts at: 387 387 Size: 5260 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5260 NotI GC'GGCC_GC Cuts at: 2707 2707 Size: 5260 NspI r_CATG'y Cuts at: 1132 1229 2618 4163 1132 Size: 97 1389 1545 2229 Fragments arranged by size: 2229 1545 1389 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5260 PciI A'CATG_T Cuts at: 4159 4159 Size: 5260 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5260 PfoI T'CCnGG_A Cuts at: 2224 2224 Size: 5260 PmeI GTTT'AAAC Cuts at: 2564 2564 Size: 5260 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3329 3361 4842 4874 3329 Size: 32 1481 32 3715 Fragments arranged by size: 3715 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5260 PsiI TTA'TAA Cuts at: 2830 2830 Size: 5260 PstI C_TGCA'G Cuts at: 830 2157 2612 830 Size: 1327 455 3478 Fragments arranged by size: 3478 1327 455 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5260 SacI G_AGCT'C Cuts at: 2559 2559 Size: 5260 SalI G'TCGA_C Cuts at: 1936 2602 1936 Size: 666 4594 SbfI CC_TGCA'GG Cuts at: 2612 2612 Size: 5260 ScaI AGT'ACT Cuts at: 3508 5220 3508 Size: 1712 3548 SfcI C'TryA_G Cuts at: 826 1045 2153 2608 3743 4424 4615 826 Size: 219 1108 455 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 681 455 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5260 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5260 SmaI CCC'GGG Cuts at: 1872 2587 1872 Size: 715 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3364 4265 4527 820 Size: 197 926 1421 901 262 1553 Fragments arranged by size: 1553 1421 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3051 493 Size: 2558 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5260 SphI G_CATG'C Cuts at: 1132 2618 1132 Size: 1486 3774 SspI AAT'ATT Cuts at: 5 52 3184 5 Size: 47 3132 2081 Fragments arranged by size: 3132 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5260 StyI C'CwwG_G Cuts at: 513 1065 2407 2699 513 Size: 552 1342 292 3074 Fragments arranged by size: 3074 1342 552 292 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2198 3287 3304 3457 3642 4087 325 Size: 1255 618 1089 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1089 618 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3506 5218 96 Size: 274 80 33 51 1443 1529 1712 138 Fragments arranged by size: 1712 1529 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2173 2205 5220 5252 2173 Size: 32 3015 32 2181 Fragments arranged by size: 3015 2181 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5260 XbaI T'CTAG_A Cuts at: 2596 2596 Size: 5260 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5260 XmaI C'CCGG_G Cuts at: 1870 2585 1870 Size: 715 4545 XmnI GAAnn'nnTTC Cuts at: 3389 3389 Size: 5260 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3068 276 Size: 53 83 186 936 1534 2468 Fragments arranged by size: 2468 1534 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI