Restriction map for
pFN21AE2630
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI Alw44I ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AvaI AvrII BamHI BanII
BbeI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiWI BsoBI BspDI
BspEI BspHI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraI DraIII
EcoO65I EcoRV EcoT22I FseI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AclI 1071 AACGTT AA^CGTT
AhdI 2365 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2317 CCTGAGG CC^T[ACGT]AGG
Aor51HI 933 AGCGCT AGC^GCT
Asp700I 1068 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2365 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1648 GAGCAC G[AT]GC[AT]^C
AviII 220 TGCGCA TGC^GCA
BanI 84 GGTGCC G^G[CT][GA]CC
BbrPI 373 CACGTG CAC^GTG
BbsI 484 GTCTTC GAAGAC(2/6)
BclI 2078 TGATCA T^GATCA
BcnI 143 CCCGG CC^[GC]GG
BpuAI 484 GTCTTC GAAGAC(2/6)
BsiEI 2052 CGGCCG CG[GA][CT]^CG
BsiHKAI 1648 GAGCAC G[AT]GC[AT]^C
BsmBI 1849 CGTCTC CGTCTC(1/5)
BsmI 2229 GAATGC GAATGC(1/-1)
Bsp1407I 1319 TGTACA T^GTACA
BspLU11I 411 ACATGT A^CATGT
BsrBI 1687 GAGCGG CCGCTC(-3/-3)
BsrDI 1693 GCAATG GCAATG(2/0)
BsrFI 1873 ACCGGC [GA]^CCGG[CT]
BsrGI 1319 TGTACA T^GTACA
BstAPI 2028 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 2058 AGATCC [GA]^GATC[CT]
Bsu36I 2317 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1873 ACCGGC [GA]^CCGG[CT]
DrdI 121 GACTGCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2052 CGGCCG C^GGCCG
Eam1105I 2365 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2052 CGGCCG C^GGCCG
Eco47III 933 AGCGCT AGC^GCT
Eco52I 2052 CGGCCG C^GGCCG
Eco81I 2317 CCTGAGG CC^T[ACGT]AGG
EcoNI 162 CCTATCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2247 GAATTC G^AATTC
FauI 1689 GCGGG CCCGC(4/6)
FbaI 2078 TGATCA T^GATCA
FspI 220 TGCGCA TGC^GCA
HgaI 686 GACGC GACGC(5/10)
HincII 988 GTCAAC GT[CT]^[GA]AC
HindII 988 GTCAAC GT[CT]^[GA]AC
MflI 2058 AGATCC [GA]^GATC[CT]
NciI 143 CCCGG CC^[GC]GG
NcoI 1055 CCATGG C^CATGG
NdeI 2162 CATATG CA^TATG
NspI 411 ACATGT [GA]CATG^[CT]
PmaCI 373 CACGTG CAC^GTG
PmlI 373 CACGTG CAC^GTG
Psp1406I 1071 AACGTT AA^CGTT
SexAI 1958 ACCAGGT A^CC[AT]GGT
SspBI 1319 TGTACA T^GTACA
SspI 2270 AATATT AAT^ATT
StuI 808 AGGCCT AGG^CCT
XcmI 1762 CCAGCCGAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2058 AGATCC [GA]^GATC[CT]
XmnI 1068 GAAAACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 411 ACATGT A^C[GA][CT]GT
878 ACGTGT
BbvCI 1364 CCTCAGC CCTCAGC(-5/-2)
1981 GCTGAGG
BglI 16 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
898 GCCACAAAGGC
BmyI 1648 GAGCAC G[AGT]GC[ACT]^C
2009 GTGCCC
BsaAI 373 CACGTG [CT]AC^GT[GA]
877 TACGTG
BsaI 379 GAGACC GGTCTC(1/5)
982 GAGACC
BseRI 198 CTCCTC GAGGAG(10/8)
1602 CTCCTC
Bsp1286I 1648 GAGCAC G[AGT]GC[ACT]^C
2009 GTGCCC
BspMI 2033 ACCTGC ACCTGC(4/8)
1416 GCAGGT
HapII 144 CCGG C^CGG
1874 CCGG
HpaII 144 CCGG C^CGG
1874 CCGG
MseI 2084 TTAA T^TAA
2173 TTAA
MspI 144 CCGG C^CGG
1874 CCGG
SapI 954 GAAGAGC GCTCTTC(1/4)
2179 GAAGAGC
SfcI 704 CTACAG C^T[GA][CT]AG
2279 CTATAG
Tru9I 2084 TTAA T^TAA
2173 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BalI BbvI
BfaI BpmI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I