(Circular) (Six-base) MAPSORT of: pFN21AE2630.seq Check: 3740 from: 1 to: 7162 pFN21AE2630 7162 bp With 182 enzymes: SgfI * June 3, 2018 17:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4972 278 Size: 53 83 186 936 3436 2468 Fragments arranged by size: 3436 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4505 1937 Size: 2568 4594 Acc65I G'GTAC_C Cuts at: 4483 4483 Size: 7162 AclI AA'CG_TT Cuts at: 1563 3077 5289 5662 1563 Size: 1514 2212 373 3063 Fragments arranged by size: 3063 2212 1514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2008 2579 2657 3809 5225 6609 6877 1632 Size: 376 571 78 1152 1416 1384 268 1917 Fragments arranged by size: 1917 1416 1384 1152 571 376 268 78 AfeI AGC'GCT Cuts at: 2940 2940 Size: 7162 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6965 AflIII A'CryG_T Cuts at: 1203 2416 2883 6061 7038 1203 Size: 1213 467 3178 977 1327 Fragments arranged by size: 3178 1327 1213 977 467 AgeI A'CCGG_T Cuts at: 5993 5993 Size: 7162 AhdI GACnn_n'nnGTC Cuts at: 4375 5891 4375 Size: 1516 5646 AleI CACnn'nnGTG Cuts at: 1166 2981 1166 Size: 1815 5347 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2291 1184 Size: 1107 6055 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3953 3985 3953 Size: 32 7130 AlwNI CAG_nnn'CTG Cuts at: 2717 4039 4318 4905 6477 2717 Size: 1322 279 587 1572 3402 Fragments arranged by size: 3402 1572 1322 587 279 ApaLI G'TGCA_C Cuts at: 5219 6375 5219 Size: 1156 6006 ApoI r'AATT_y Cuts at: 1493 3116 3552 4252 4471 4697 4849 6958 1493 Size: 1623 436 700 219 226 152 2109 1697 Fragments arranged by size: 2109 1697 1623 700 436 226 219 152 AseI AT'TA_AT Cuts at: 160 1032 5716 160 Size: 872 4684 1606 Fragments arranged by size: 4684 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 AvaI C'yCGr_G Cuts at: 1870 1943 4487 1870 Size: 73 2544 4545 Fragments arranged by size: 4545 2544 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4019 4052 4019 Size: 33 7129 BamHI G'GATC_C Cuts at: 4492 4859 4492 Size: 367 6795 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2089 4483 5938 618 Size: 325 217 627 302 2394 1455 1842 Fragments arranged by size: 2394 1842 1455 627 325 302 217 BanII G_rGCy'C Cuts at: 1391 4481 1391 Size: 3090 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2482 928 Size: 971 583 5608 Fragments arranged by size: 5608 971 583 BbvCI CC'TCA_GC Cuts at: 3370 3987 3370 Size: 617 6545 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5353 5387 1903 Size: 34 50 34 3332 34 3678 Fragments arranged by size: 3678 3332 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5054 6264 5054 Size: 1210 5952 BclI T'GATC_A Cuts at: 1548 4083 1548 Size: 2535 4627 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 2909 5773 Size: 107 122 71 631 605 355 882 2864 Cuts at: 5773 136 Size: 1525 Fragments arranged by size: 2864 1525 882 631 605 355 122 107 71 BglII A'GATC_T Cuts at: 7157 7157 Size: 7162 BlpI GC'TnA_GC Cuts at: 4579 4579 Size: 7162 Bme1580I G_kGCm'C Cuts at: 1790 4018 5223 6379 1790 Size: 2228 1205 1156 2573 Fragments arranged by size: 2573 2228 1205 1156 BmgBI CAC'GTC Cuts at: 1318 2401 3940 1318 Size: 1083 1539 4540 Fragments arranged by size: 4540 1539 1083 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3797 4893 5851 Size: 416 53 83 399 24 2380 1096 958 Cuts at: 5851 442 Size: 1753 Fragments arranged by size: 2380 1753 1096 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7162 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2080 3223 3239 3519 5822 1239 Size: 841 1143 16 280 2303 2579 Fragments arranged by size: 2579 2303 1143 841 280 16 Bpu10I CC'TnA_GC Cuts at: 3370 3705 3987 3370 Size: 335 282 6545 Fragments arranged by size: 6545 335 282 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5287 6152 6450 5287 Size: 865 298 5999 Fragments arranged by size: 5999 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2378 2981 5825 882 Size: 617 879 603 2844 2219 Fragments arranged by size: 2844 2219 879 617 603 BsaAI yAC'GTr Cuts at: 493 1204 2380 2884 4953 493 Size: 711 1176 504 2069 2702 Fragments arranged by size: 2702 2069 1176 711 504 BsaBI GATnn'nnATC Cuts at: 7156 7156 Size: 7162 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4969 5351 5986 275 Size: 53 83 186 936 3436 382 635 1451 Fragments arranged by size: 3436 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5595 5993 6267 6414 6825 5595 Size: 398 274 147 411 5932 Fragments arranged by size: 5932 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6937 Fragments arranged by size: 6937 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2194 3598 1186 Size: 1008 1404 4750 Fragments arranged by size: 4750 1404 1008 BseYI C'CCAG_C Cuts at: 2463 2780 2941 2969 4052 4189 6365 6861 2463 Size: 317 161 28 1083 137 2176 496 2764 Fragments arranged by size: 2764 2176 1083 496 317 161 137 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 3440 4025 4176 1163 Size: 573 561 1143 585 151 4149 Fragments arranged by size: 4149 1143 585 573 561 151 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4060 4612 5373 6401 1540 Size: 275 186 2059 552 761 1028 2301 Fragments arranged by size: 2301 2059 1028 761 552 275 186 BsiHKAI G_wGCw'C Cuts at: 3657 5223 5308 6379 3657 Size: 1566 85 1071 4440 Fragments arranged by size: 4440 1566 1071 85 BsmI GAATG_Cn' Cuts at: 4240 4673 4766 4240 Size: 433 93 6636 Fragments arranged by size: 6636 433 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3860 3860 Size: 7162 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3657 4018 4481 5223 5308 6379 1391 Size: 399 1867 361 463 742 85 1071 2174 Fragments arranged by size: 2174 1867 1071 742 463 399 361 85 BspEI T'CCGG_A Cuts at: 6825 6825 Size: 7162 BspHI T'CATG_A Cuts at: 5051 6781 5051 Size: 1730 5432 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3412 4047 4517 844 Size: 2568 635 470 3489 Fragments arranged by size: 3489 2568 635 470 BsrBI CCG'CTC Cuts at: 1394 1823 3694 5049 1394 Size: 429 1871 1355 3507 Fragments arranged by size: 3507 1871 1355 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3705 5657 5831 65 Size: 1183 2457 1952 174 1396 Fragments arranged by size: 2457 1952 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3878 5806 5993 1700 Size: 110 2068 1928 187 2869 Fragments arranged by size: 2869 2068 1928 187 110 BsrGI T'GTAC_A Cuts at: 96 3324 96 Size: 3228 3934 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7162 BssSI C'ACGA_G Cuts at: 5222 6234 5222 Size: 1012 6150 BstAPI GCAn_nnn'nTGC Cuts at: 794 4039 794 Size: 3245 3917 BstBI TT'CG_AA Cuts at: 5966 5966 Size: 7162 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7162 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2528 3067 3557 3586 2248 Size: 280 539 490 29 5824 Fragments arranged by size: 5824 539 490 280 29 BstYI r'GATC_y Cuts at: 1972 4063 4492 4859 5244 5261 6713 6799 6811 Size: 2091 429 367 385 17 1452 86 12 Cuts at: 6811 7157 1972 Size: 346 1977 Fragments arranged by size: 2091 1977 1452 429 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4323 4323 Size: 7162 BtgI C'CryG_G Cuts at: 513 1065 2104 2317 3060 3999 513 Size: 552 1039 213 743 939 3676 Fragments arranged by size: 3676 1039 939 743 552 213 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6423 Fragments arranged by size: 6423 683 56 BtsI GCAGTG_nn' Cuts at: 3390 3591 4218 4678 5472 5492 3390 Size: 201 627 460 794 20 5060 Fragments arranged by size: 5060 794 627 460 201 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7162 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7127 DraI TTT'AAA Cuts at: 4466 4466 Size: 7162 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2132 6169 809 Size: 507 816 4037 1802 Fragments arranged by size: 4037 1802 816 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2686 3012 Size: 54 1425 50 275 18 327 529 326 Cuts at: 3012 3462 3714 3750 3880 4057 4609 5498 7127 Size: 450 252 36 130 177 552 889 1629 Cuts at: 7127 8 Size: 43 Fragments arranged by size: 1629 1425 889 552 529 450 327 326 275 252 177 130 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 4057 4609 1537 Size: 275 2245 552 4090 Fragments arranged by size: 4090 2245 552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2954 3551 4179 5092 1353 Size: 1601 597 628 913 3423 Fragments arranged by size: 3423 1601 913 628 597 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5749 6123 6269 387 Size: 1064 138 4160 374 146 1280 Fragments arranged by size: 4160 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2008 2080 2579 2657 3223 3239 3519 Size: 393 376 72 499 78 566 16 280 Cuts at: 3519 3809 5225 5822 6609 6877 1239 Size: 290 1416 597 787 268 1524 Fragments arranged by size: 1524 1416 787 597 566 499 393 376 290 280 268 78 72 16 EcoNI CCTnn'n_nnAGG Cuts at: 2171 2171 Size: 7162 EcoO109I rG'GnC_Cy Cuts at: 2668 3781 4138 2668 Size: 1113 357 5692 Fragments arranged by size: 5692 1113 357 EcoRI G'AATT_C Cuts at: 4252 4471 4849 4252 Size: 219 378 6565 Fragments arranged by size: 6565 378 219 FspI TGC'GCA Cuts at: 2227 5668 2227 Size: 3441 3721 HaeII r_GCGC'y Cuts at: 1678 2087 2103 2557 2942 6309 1678 Size: 409 16 454 385 3367 2531 Fragments arranged by size: 3367 2531 454 409 385 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2472 2504 2716 2748 3833 Size: 32 135 32 900 32 212 32 1085 Cuts at: 3833 3865 4361 4393 5803 5835 5877 5909 1373 Size: 32 496 32 1410 32 42 32 2626 Fragments arranged by size: 2626 1410 1085 900 496 212 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2995 4506 4752 669 Size: 988 281 1057 1511 246 3079 Fragments arranged by size: 3079 1511 1057 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7162 HpaI GTT'AAC Cuts at: 4752 4752 Size: 7162 KpnI G_GTAC'C Cuts at: 4487 4487 Size: 7162 MfeI C'AATT_G Cuts at: 4761 4761 Size: 7162 MluI A'CGCG_T Cuts at: 7038 7038 Size: 7162 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6276 6460 1305 Size: 4971 184 2007 Fragments arranged by size: 4971 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2159 3464 3716 10 Size: 54 1425 343 327 1305 252 3456 Fragments arranged by size: 3456 1425 1305 343 327 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 2954 2981 3561 5120 5479 5638 518 Size: 648 1788 27 580 1559 359 159 2042 Fragments arranged by size: 2042 1788 1559 648 580 359 159 27 MspA1I CmG'CkG Cuts at: 1606 2248 2780 2788 3028 4056 4578 5255 6403 Size: 642 532 8 240 1028 522 677 1148 Cuts at: 6403 6648 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1028 677 642 532 522 245 240 8 NcoI C'CATG_G Cuts at: 513 1065 3060 513 Size: 552 1995 4615 Fragments arranged by size: 4615 1995 552 NdeI CA'TA_TG Cuts at: 387 4168 387 Size: 3781 3381 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7162 NotI GC'GGCC_GC Cuts at: 4609 4609 Size: 7162 NspI r_CATG'y Cuts at: 1132 1229 2420 4520 6065 1132 Size: 97 1191 2100 1545 2229 Fragments arranged by size: 2229 2100 1545 1191 97 PasI CC'CwG_GG Cuts at: 1348 2045 1348 Size: 697 6465 PciI A'CATG_T Cuts at: 2416 6061 2416 Size: 3645 3517 PflMI CCAn_nnn'nTGG Cuts at: 1108 3061 3385 3460 1108 Size: 1953 324 75 4810 Fragments arranged by size: 4810 1953 324 75 PfoI T'CCnGG_A Cuts at: 2823 2856 3108 3506 2823 Size: 33 252 398 6479 Fragments arranged by size: 6479 398 252 33 PmeI GTTT'AAAC Cuts at: 4466 4466 Size: 7162 PmlI CAC'GTG Cuts at: 2380 2380 Size: 7162 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5231 5263 6744 6776 5231 Size: 32 1481 32 5617 Fragments arranged by size: 5617 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2668 3781 4138 2668 Size: 1113 357 5692 Fragments arranged by size: 5692 1113 357 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7162 PsiI TTA'TAA Cuts at: 4732 4732 Size: 7162 PstI C_TGCA'G Cuts at: 830 4514 830 Size: 3684 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7162 PvuII CAG'CTG Cuts at: 2248 2780 2788 3028 2248 Size: 532 8 240 6382 Fragments arranged by size: 6382 532 240 8 SalI G'TCGA_C Cuts at: 1936 4504 1936 Size: 2568 4594 SanDI GG'GwC_CC Cuts at: 4138 4138 Size: 7162 SapI GCTCTTCn'nnn_ Cuts at: 2954 4179 2954 Size: 1225 5937 SbfI CC_TGCA'GG Cuts at: 4514 4514 Size: 7162 ScaI AGT'ACT Cuts at: 5410 7122 5410 Size: 1712 5450 SexAI A'CCwGG_T Cuts at: 3963 3963 Size: 7162 SfcI C'TryA_G Cuts at: 826 1045 2709 4284 4510 5645 6326 6517 826 Size: 219 1664 1575 226 1135 681 191 1471 Fragments arranged by size: 1664 1575 1471 1135 681 226 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7162 SmaI CCC'GGG Cuts at: 1872 4489 1872 Size: 2617 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5266 6167 6429 820 Size: 197 926 3323 901 262 1553 Fragments arranged by size: 3323 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4953 493 Size: 4460 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7162 SphI G_CATG'C Cuts at: 1132 4520 1132 Size: 3388 3774 SspI AAT'ATT Cuts at: 5 52 4277 5086 5 Size: 47 4225 809 2081 Fragments arranged by size: 4225 2081 809 47 StuI AGG'CCT Cuts at: 1517 2815 1517 Size: 1298 5864 StyI C'CwwG_G Cuts at: 513 1065 2503 2683 3060 4601 513 Size: 552 1438 180 377 1541 3074 Fragments arranged by size: 3074 1541 1438 552 377 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3929 5189 5206 5359 5544 5989 325 Size: 1255 2349 1260 17 153 185 445 1498 Fragments arranged by size: 2349 1498 1260 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3324 5408 7120 Size: 274 80 33 51 1443 1347 2084 1712 Cuts at: 7120 96 Size: 138 Fragments arranged by size: 2084 1712 1443 1347 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7122 7154 7122 Size: 32 7130 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7162 XbaI T'CTAG_A Cuts at: 4498 4498 Size: 7162 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3774 3774 Size: 7162 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7162 XmaI C'CCGG_G Cuts at: 1870 4487 1870 Size: 2617 4545 XmnI GAAnn'nnTTC Cuts at: 3077 5291 3077 Size: 2214 4948 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4970 276 Size: 53 83 186 936 3436 2468 Fragments arranged by size: 3436 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI