Restriction map for
pFN21AE2630
(Linear) (Six-base) MAPSORT of: KIEE2630.seq Check: 7557 from: 1 to: 2457
KIEE2630.orf
With 182 enzymes: SgfI *
June 3, 2018 14:24 ..
AclI AA'CG_TT
Cuts at: 0 1072 2457
Size: 1072 1385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 574 652 1804 2457
Size: 3 571 78 1152 653
Fragments arranged by size:
1152 653 571 78 3
AfeI AGC'GCT
Cuts at: 0 935 2457
Size: 935 1522
AflIII A'CryG_T
Cuts at: 0 411 878 2457
Size: 411 467 1579
Fragments arranged by size:
1579 467 411
AhdI GACnn_n'nnGTC
Cuts at: 0 2370 2457
Size: 2370 87
AleI CACnn'nnGTG
Cuts at: 0 976 2457
Size: 976 1481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 286 2457
Size: 286 2171
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1948 1980 2457
Size: 1948 32 477
Fragments arranged by size:
1948 477 32
AlwNI CAG_nnn'CTG
Cuts at: 0 712 2034 2313 2457
Size: 712 1322 279 144
Fragments arranged by size:
1322 712 279 144
ApoI r'AATT_y
Cuts at: 0 1111 1547 2247 2457
Size: 1111 436 700 210
Fragments arranged by size:
1111 700 436 210
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2014 2047 2457
Size: 2014 33 410
Fragments arranged by size:
2014 410 33
BanI G'GyrC_C
Cuts at: 0 84 2457
Size: 84 2373
BbsI GAAGACnn'nnnn_
Cuts at: 0 477 2457
Size: 477 1980
BbvCI CC'TCA_GC
Cuts at: 0 1365 1982 2457
Size: 1365 617 475
Fragments arranged by size:
1365 617 475
BclI T'GATC_A
Cuts at: 0 2078 2457
Size: 2078 379
BglI GCCn_nnn'nGGC
Cuts at: 0 22 904 2457
Size: 22 882 1553
Fragments arranged by size:
1553 882 22
Bme1580I G_kGCm'C
Cuts at: 0 2013 2457
Size: 2013 444
BmgBI CAC'GTC
Cuts at: 0 396 1935 2457
Size: 396 1539 522
Fragments arranged by size:
1539 522 396
BmrI ACTGGGnnnn_n'
Cuts at: 0 1792 2457
Size: 1792 665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 1218 1234 1514 2457
Size: 75 1143 16 280 943
Fragments arranged by size:
1143 943 280 75 16
Bpu10I CC'TnA_GC
Cuts at: 0 1365 1700 1982 2457
Size: 1365 335 282 475
Fragments arranged by size:
1365 475 335 282
BsaI GGTCTCn'nnnn_
Cuts at: 0 373 976 2457
Size: 373 603 1481
Fragments arranged by size:
1481 603 373
BsaAI yAC'GTr
Cuts at: 0 375 879 2457
Size: 375 504 1578
Fragments arranged by size:
1578 504 375
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 189 1593 2457
Size: 189 1404 864
Fragments arranged by size:
1404 864 189
BseYI C'CCAG_C
Cuts at: 0 458 775 936 964 2047 2184 2457
Size: 458 317 161 28 1083 137 273
Fragments arranged by size:
1083 458 317 273 161 137 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1435 2020 2171 2457
Size: 292 1143 585 151 286
Fragments arranged by size:
1143 585 292 286 151
BsiEI CG_ry'CG
Cuts at: 0 2055 2457
Size: 2055 402
BsiHKAI G_wGCw'C
Cuts at: 0 1652 2457
Size: 1652 805
BsmI GAATG_Cn'
Cuts at: 0 2235 2457
Size: 2235 222
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1855 2457
Size: 1855 602
Bsp1286I G_dGCh'C
Cuts at: 0 1652 2013 2457
Size: 1652 361 444
Fragments arranged by size:
1652 444 361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1407 2042 2457
Size: 1407 635 415
Fragments arranged by size:
1407 635 415
BsrBI CCG'CTC
Cuts at: 0 1689 2457
Size: 1689 768
BsrDI GCAATG_nn'
Cuts at: 0 1700 2457
Size: 1700 757
BsrFI r'CCGG_y
Cuts at: 0 1873 2457
Size: 1873 584
BsrGI T'GTAC_A
Cuts at: 0 1319 2457
Size: 1319 1138
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2034 2457
Size: 2034 423
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 523 1062 1552 1581 2457
Size: 243 280 539 490 29 876
Fragments arranged by size:
876 539 490 280 243 29
BstYI r'GATC_y
Cuts at: 0 2058 2457
Size: 2058 399
Bsu36I CC'TnA_GG
Cuts at: 0 2318 2457
Size: 2318 139
BtgI C'CryG_G
Cuts at: 0 99 312 1055 1994 2457
Size: 99 213 743 939 463
Fragments arranged by size:
939 743 463 213 99
BtsI GCAGTG_nn'
Cuts at: 0 1385 1586 2213 2457
Size: 1385 201 627 244
Fragments arranged by size:
1385 627 244 201
DrdI GACnn_nn'nnGTC
Cuts at: 0 127 2457
Size: 127 2330
EaeI y'GGCC_r
Cuts at: 0 152 681 1007 1457 1709 1745 1875 2052
Size: 152 529 326 450 252 36 130 177
Cuts at: 2052 2457
Size: 405
Fragments arranged by size:
529 450 405 326 252 177 152 130
36
EagI C'GGCC_G
Cuts at: 0 2052 2457
Size: 2052 405
EarI CTCTTCn'nnn_
Cuts at: 0 949 1546 2174 2457
Size: 949 597 628 283
Fragments arranged by size:
949 628 597 283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 75 574 652 1218 1234 1514 1804
Size: 3 72 499 78 566 16 280 290
Cuts at: 1804 2457
Size: 653
Fragments arranged by size:
653 566 499 290 280 78 72 16
3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 166 2457
Size: 166 2291
EcoO109I rG'GnC_Cy
Cuts at: 0 663 1776 2133 2457
Size: 663 1113 357 324
Fragments arranged by size:
1113 663 357 324
EcoRI G'AATT_C
Cuts at: 0 2247 2457
Size: 2247 210
FspI TGC'GCA
Cuts at: 0 222 2457
Size: 222 2235
HaeII r_GCGC'y
Cuts at: 0 82 98 552 937 2457
Size: 82 16 454 385 1520
Fragments arranged by size:
1520 454 385 82 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 467 499 711 743 1828 1860 2356 2388
Size: 467 32 212 32 1085 32 496 32
Cuts at: 2388 2457
Size: 69
Fragments arranged by size:
1085 496 467 212 69 32 32 32
32
HincII GTy'rAC
Cuts at: 0 990 2457
Size: 990 1467
MscI TGG'CCA
Cuts at: 0 154 1459 1711 2457
Size: 154 1305 252 746
Fragments arranged by size:
1305 746 252 154
MslI CAynn'nnrTG
Cuts at: 0 949 976 1556 2457
Size: 949 27 580 901
Fragments arranged by size:
949 901 580 27
MspA1I CmG'CkG
Cuts at: 0 243 775 783 1023 2051 2457
Size: 243 532 8 240 1028 406
Fragments arranged by size:
1028 532 406 243 240 8
NcoI C'CATG_G
Cuts at: 0 1055 2457
Size: 1055 1402
NdeI CA'TA_TG
Cuts at: 0 2163 2457
Size: 2163 294
NspI r_CATG'y
Cuts at: 0 415 2457
Size: 415 2042
PasI CC'CwG_GG
Cuts at: 0 40 2457
Size: 40 2417
PciI A'CATG_T
Cuts at: 0 411 2457
Size: 411 2046
PflMI CCAn_nnn'nTGG
Cuts at: 0 1056 1380 1455 2457
Size: 1056 324 75 1002
Fragments arranged by size:
1056 1002 324 75
PfoI T'CCnGG_A
Cuts at: 0 818 851 1103 1501 2457
Size: 818 33 252 398 956
Fragments arranged by size:
956 818 398 252 33
PmlI CAC'GTG
Cuts at: 0 375 2457
Size: 375 2082
PpuMI rG'GwC_Cy
Cuts at: 0 663 1776 2133 2457
Size: 663 1113 357 324
Fragments arranged by size:
1113 663 357 324
PvuII CAG'CTG
Cuts at: 0 243 775 783 1023 2457
Size: 243 532 8 240 1434
Fragments arranged by size:
1434 532 243 240 8
SanDI GG'GwC_CC
Cuts at: 0 2133 2457
Size: 2133 324
SapI GCTCTTCn'nnn_
Cuts at: 0 949 2174 2457
Size: 949 1225 283
Fragments arranged by size:
1225 949 283
SexAI A'CCwGG_T
Cuts at: 0 1958 2457
Size: 1958 499
SfcI C'TryA_G
Cuts at: 0 704 2279 2457
Size: 704 1575 178
Fragments arranged by size:
1575 704 178
SspI AAT'ATT
Cuts at: 0 2272 2457
Size: 2272 185
StuI AGG'CCT
Cuts at: 0 810 2457
Size: 810 1647
StyI C'CwwG_G
Cuts at: 0 498 678 1055 2457
Size: 498 180 377 1402
Fragments arranged by size:
1402 498 377 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1924 2457
Size: 1924 533
TatI w'GTAC_w
Cuts at: 0 1319 2457
Size: 1319 1138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1769 2457
Size: 1769 688
XmnI GAAnn'nnTTC
Cuts at: 0 1072 2457
Size: 1072 1385
Enzymes that do cut:
AclI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApoI BaeI BanI BbsI BbvCI BclI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DrdI EaeI EagI EarI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII MscI MslI
MspA1I NcoI NdeI NspI PasI PciI PflMI PfoI
PmlI PpuMI PvuII SanDI SapI SexAI SfcI SspI
StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AgeI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BamHI BanII BbeI
BcgI BciVI BfrBI BglII BlpI BmtI BplI BpuEI
BsaBI BsaHI BsaWI BsaXI BsiWI BspEI BspHI BssHII
BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII EciI EcoICRI EcoRV FalI FseI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI
TstI Tth111I XbaI XhoI XmaI ZraI