################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvrII BciVI BfrI BglII BlnI BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoRV EcoT22I FseI HincII HindII HpaI KpnI MamI MfeI MluI MslI MunI NdeI NruI NsiI NspI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfuI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccIII 2810 TCCGGA T^CCGGA AocI 1165 CCTAAGG CC^T[ACGT]AGG Asp700I 1452 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 150 GGATCC G^GATCC BbrPI 2862 CACGTG CAC^GTG BcgI 1875 CGAGGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1175 TGATCA T^GATCA BsaAI 2862 CACGTG [CT]AC^GT[GA] BseAI 2810 TCCGGA T^CCGGA BspEI 2810 TCCGGA T^CCGGA BspMI 2384 ACCTGC ACCTGC(4/8) BsrDI 2739 GCAATG GCAATG(2/0) BssHII 17 GCGCGC G^CGCGC Bsu36I 1165 CCTAAGG CC^T[ACGT]AGG Eco81I 1165 CCTAAGG CC^T[ACGT]AGG EcoNI 802 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2826 GAATTC G^AATTC FbaI 1175 TGATCA T^GATCA HindIII 1983 AAGCTT A^AGCTT KspI 110 CCGCGG CCGC^GG MroI 2810 TCCGGA T^CCGGA NheI 3538 GCTAGC G^CTAGC NotI 106 GCGGCCGC GC^GGCCGC PaeR7I 70 CTCGAG C^TCGAG PmaCI 2862 CACGTG CAC^GTG PmlI 2862 CACGTG CAC^GTG SacII 110 CCGCGG CCGC^GG SexAI 1292 ACCAGGT A^CC[AT]GGT SfiI 291 GGCCAGTCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 123 CGCCGGTG C[GA]^CCGG[CT]G SspI 2602 AATATT AAT^ATT XhoI 70 CTCGAG C^TCGAG XmnI 1452 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 2403 GAATTT [GA]^AATT[CT] 2826 GAATTC ApaI 1087 GGGCCC GGGCC^C 2849 GGGCCC ApoI 2403 GAATTT [GA]^AATT[CT] 2826 GAATTC AviII 1706 TGCGCA TGC^GCA 2768 TGCGCA BbvCI 2246 CCTCAGC CCTCAGC(-5/-2) 2418 CCTCAGC BlpI 432 GCTCAGC GC^T[ACGT]AGC 3138 GCTGAGC Bpu1102I 432 GCTCAGC GC^T[ACGT]AGC 3138 GCTGAGC BsaI 2444 GAGACC GGTCTC(1/5) 699 GGTCTC BsmBI 202 CGTCTC CGTCTC(1/5) 1474 CGTCTC BstAPI 1387 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3321 GCAACTGCTGC BstEII 1698 GGTGACC G^GT[ACGT]ACC 1855 GGTCACC BstPI 1698 GGTGACC G^GT[ACGT]ACC 1855 GGTCACC BstYI 150 GGATCC [GA]^GATC[CT] 2723 AGATCC CelII 432 GCTCAGC GC^T[ACGT]AGC 3138 GCTGAGC EcoO65I 1698 GGTGACC G^GT[ACGT]ACC 1855 GGTCACC FspI 1706 TGCGCA TGC^GCA 2768 TGCGCA HgaI 3361 GACGC GACGC(5/10) 3556 GACGC MflI 150 GGATCC [GA]^GATC[CT] 2723 AGATCC NcoI 1479 CCATGG C^CATGG 2483 CCATGG StuI 1518 AGGCCT AGG^CCT 2301 AGGCCT XcmI 1130 CCAGGGAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2870 CCAGCTTGAACCTGG XhoII 150 GGATCC [GA]^GATC[CT] 2723 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XmaCI XmaI