Restriction map for
pFN21AE2738
(Circular) (Six-base) MAPSORT of: pFN21AE2738.seq Check: 5130 from: 1 to: 8281
pFN21AE2738 8281 bp
With 182 enzymes: SgfI *
December 20, 2018 17:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4398 4398
Size: 8281
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6091 278
Size: 53 83 186 936 4555 2468
Fragments arranged by size:
4555 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5624 1937
Size: 3687 4594
Acc65I G'GTAC_C
Cuts at: 5602 5602
Size: 8281
AclI AA'CG_TT
Cuts at: 1563 6408 6781 1563
Size: 4845 373 3063
Fragments arranged by size:
4845 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2012 3424 4415 4557 4578 6344 7728 7996
Size: 380 1412 991 142 21 1766 1384 268
Cuts at: 7996 1632
Size: 1917
Fragments arranged by size:
1917 1766 1412 1384 991 380 268 142
21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8084
AflIII A'CryG_T
Cuts at: 1203 7180 8157 1203
Size: 5977 977 1327
Fragments arranged by size:
5977 1327 977
AgeI A'CCGG_T
Cuts at: 7112 7112
Size: 8281
AhdI GACnn_n'nnGTC
Cuts at: 7010 7010
Size: 8281
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3764 1184
Size: 2580 5701
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2196 2228 2196
Size: 32 8249
AlwNI CAG_nnn'CTG
Cuts at: 2912 3304 3425 3730 3896 5128 6024 7596 2912
Size: 392 121 305 166 1232 896 1572 3597
Fragments arranged by size:
3597 1572 1232 896 392 305 166 121
ApaI G_GGCC'C
Cuts at: 3096 4858 3096
Size: 1762 6519
ApaLI G'TGCA_C
Cuts at: 6338 7494 6338
Size: 1156 7125
ApoI r'AATT_y
Cuts at: 1493 4408 4831 5590 5816 5968 8077 1493
Size: 2915 423 759 226 152 2109 1697
Fragments arranged by size:
2915 2109 1697 759 423 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6835 160
Size: 872 5803 1606
Fragments arranged by size:
5803 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8281
AvaI C'yCGr_G
Cuts at: 1870 1943 2075 2169 2446 2790 2942 3576 4210
Size: 73 132 94 277 344 152 634 634
Cuts at: 4210 4225 4416 5449 5606 1870
Size: 15 191 1033 157 4545
Fragments arranged by size:
4545 1033 634 634 344 277 191 157
152 132 94 73 15
BamHI G'GATC_C
Cuts at: 2155 5611 5978 2155
Size: 3456 367 4458
Fragments arranged by size:
4458 3456 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2083 2123 2132 2255 2408
Size: 325 217 627 296 40 9 123 153
Cuts at: 2408 2427 2519 2545 3149 3391 4889 5137 5602
Size: 19 92 26 604 242 1498 248 465
Cuts at: 5602 7057 618
Size: 1455 1842
Fragments arranged by size:
1842 1498 1455 627 604 465 325 296
248 242 217 153 123 92 40 26
19 9
BanII G_rGCy'C
Cuts at: 1391 2274 2390 2469 2747 2791 3096 3159 3607
Size: 883 116 79 278 44 305 63 448
Cuts at: 3607 3663 3873 4272 4423 4460 4858 5307 5600
Size: 56 210 399 151 37 398 449 293
Cuts at: 5600 1391
Size: 4072
Fragments arranged by size:
4072 883 449 448 399 398 305 293
278 210 151 116 79 63 56 44
37
BbeI G_GCGC'C
Cuts at: 2127 2412 2431 2549 3153 4893 5141 2127
Size: 285 19 118 604 1740 248 5267
Fragments arranged by size:
5267 1740 604 285 248 118 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3111 3317 3430 3670 928
Size: 971 1212 206 113 240 5539
Fragments arranged by size:
5539 1212 971 240 206 113
BbvCI CC'TCA_GC
Cuts at: 4252 4424 4252
Size: 172 8109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3869 3903 6472 6506 1903
Size: 34 1932 34 2569 34 3678
Fragments arranged by size:
3678 2569 1932 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6173 7383 6173
Size: 1210 7071
BclI T'GATC_A
Cuts at: 1548 3180 1548
Size: 1632 6649
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2172 2303 2481
Size: 107 122 71 631 605 500 131 178
Cuts at: 2481 2944 4808 5192 5402 6892 136
Size: 463 1864 384 210 1490 1525
Fragments arranged by size:
1864 1525 1490 631 605 500 463 384
210 178 131 122 107 71
BglII A'GATC_T
Cuts at: 8276 8276
Size: 8281
BlpI GC'TnA_GC
Cuts at: 2438 5144 5698 2438
Size: 2706 554 5021
Fragments arranged by size:
5021 2706 554
Bme1580I G_kGCm'C
Cuts at: 1790 2258 3096 3394 4858 4937 6342 7498 1790
Size: 468 838 298 1464 79 1405 1156 2573
Fragments arranged by size:
2573 1464 1405 1156 838 468 298 79
BmgBI CAC'GTC
Cuts at: 1318 4665 5203 5314 5353 1318
Size: 3347 538 111 39 4246
Fragments arranged by size:
4246 3347 538 111 39
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2160 2306 4134
Size: 416 53 83 399 24 743 146 1828
Cuts at: 4134 4344 6012 6970 442
Size: 210 1668 958 1753
Fragments arranged by size:
1828 1753 1668 958 743 416 399 210
146 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5547 1056
Size: 4491 3790
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2715 2805 2958 2987 3326 3467 4467 5420
Size: 1476 90 153 29 339 141 1000 953
Cuts at: 5420 6941 1239
Size: 1521 2579
Fragments arranged by size:
2579 1521 1476 1000 953 339 153 141
90 29
Bpu10I CC'TnA_GC
Cuts at: 4252 4424 4454 4252
Size: 172 30 8079
Fragments arranged by size:
8079 172 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2522 3023 6406 7271 7569 2522
Size: 501 3383 865 298 3234
Fragments arranged by size:
3383 3234 865 501 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2710 4443 6944 882
Size: 617 1211 1733 2501 2219
Fragments arranged by size:
2501 2219 1733 1211 617
BsaAI yAC'GTr
Cuts at: 493 1204 4869 6072 493
Size: 711 3665 1203 2702
Fragments arranged by size:
3665 2702 1203 711
BsaBI GATnn'nnATC
Cuts at: 8275 8275
Size: 8281
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 2409 2428 2546
Size: 53 83 186 936 591 285 19 118
Cuts at: 2546 3150 4890 5138 5367 5562 6088 6470 7105
Size: 604 1740 248 229 195 526 382 635
Cuts at: 7105 275
Size: 1451
Fragments arranged by size:
1740 1451 936 635 604 591 526 382
285 248 229 195 186 118 83 53
19
BsaWI w'CCGG_w
Cuts at: 3784 4404 4815 6714 7112 7386 7533 7944 3784
Size: 620 411 1899 398 274 147 411 4121
Fragments arranged by size:
4121 1899 620 411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2688 2718 5081 5111 1178
Size: 30 165 30 1285 30 2363 30 4348
Fragments arranged by size:
4348 2363 1285 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 2410 2541 2862 2865 3534 4519 4706
Size: 1009 215 131 321 3 669 985 187
Cuts at: 4706 5102 5171 1186
Size: 396 69 4296
Fragments arranged by size:
4296 1009 985 669 396 321 215 187
131 69 3
BseYI C'CCAG_C
Cuts at: 2275 2451 2777 3110 3145 3238 3386 3414 3752
Size: 176 326 333 35 93 148 28 338
Cuts at: 3752 4343 4547 5164 5480 5498 5516 7484 7980
Size: 591 204 617 316 18 18 1968 496
Cuts at: 7980 2275
Size: 2576
Fragments arranged by size:
2576 1968 617 591 496 338 333 326
316 204 176 148 93 35 28 18
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2934 3905 5210 1163
Size: 573 1198 971 1305 4234
Fragments arranged by size:
4234 1305 1198 971 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2115 2121 2332 5301 5731 6492
Size: 275 186 114 6 211 2969 430 761
Cuts at: 6492 7520 1540
Size: 1028 2301
Fragments arranged by size:
2969 2301 1028 761 430 275 211 186
114 6
BsiHKAI G_wGCw'C
Cuts at: 3071 3159 3607 3873 3908 4289 5150 5307 6342
Size: 88 448 266 35 381 861 157 1035
Cuts at: 6342 6427 7498 3071
Size: 85 1071 3854
Fragments arranged by size:
3854 1071 1035 861 448 381 266 157
88 85 35
BsmI GAATG_Cn'
Cuts at: 5792 5885 5792
Size: 93 8188
BsmBI CGTCTCn'nnnn_
Cuts at: 2213 3485 2213
Size: 1272 7009
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2258 2274 2390 2469 2747 2791 3071
Size: 399 468 16 116 79 278 44 280
Cuts at: 3071 3096 3159 3394 3607 3663 3873 3908 4272
Size: 25 63 235 213 56 210 35 364
Cuts at: 4272 4289 4423 4460 4858 4937 5150 5307 5600
Size: 17 134 37 398 79 213 157 293
Cuts at: 5600 6342 6427 7498 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 468 399 398 364 293
280 278 235 213 213 210 157 134
116 85 79 79 63 56 44 37
35 25 17 16
BspEI T'CCGG_A
Cuts at: 4815 7944 4815
Size: 3129 5152
BspHI T'CATG_A
Cuts at: 6170 7900 6170
Size: 1730 6551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4398 5636 844
Size: 3554 1238 3489
Fragments arranged by size:
3554 3489 1238
BsrBI CCG'CTC
Cuts at: 1394 1823 2324 2371 3210 4696 5248 6168 1394
Size: 429 501 47 839 1486 552 920 3507
Fragments arranged by size:
3507 1486 920 839 552 501 429 47
BsrDI GCAATG_nn'
Cuts at: 65 1248 4751 6776 6950 65
Size: 1183 3503 2025 174 1396
Fragments arranged by size:
3503 2025 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 2129 2217 2522 2557 2603 2864
Size: 110 310 9 88 305 35 46 261
Cuts at: 2864 6925 7112 1700
Size: 4061 187 2869
Fragments arranged by size:
4061 2869 310 305 261 187 110 88
46 35 9
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8281
BssHII G'CGCG_C
Cuts at: 1925 2022 1925
Size: 97 8184
BssSI C'ACGA_G
Cuts at: 2242 2587 3021 3594 6341 7353 2242
Size: 345 434 573 2747 1012 3170
Fragments arranged by size:
3170 2747 1012 573 434 345
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3398 5332 794
Size: 2604 1934 3743
Fragments arranged by size:
3743 2604 1934
BstBI TT'CG_AA
Cuts at: 7085 7085
Size: 8281
BstEII G'GTnAC_C
Cuts at: 1184 3703 3860 1184
Size: 2519 157 5605
Fragments arranged by size:
5605 2519 157
BstYI r'GATC_y
Cuts at: 1972 2155 4728 5611 5978 6363 6380 7832 7918
Size: 183 2573 883 367 385 17 1452 86
Cuts at: 7918 7930 8276 1972
Size: 12 346 1977
Fragments arranged by size:
2573 1977 1452 883 385 367 346 183
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3171 3171
Size: 8281
BtgI C'CryG_G
Cuts at: 513 1065 2115 3484 4168 4488 4797 4979 513
Size: 552 1050 1369 684 320 309 182 3815
Fragments arranged by size:
3815 1369 1050 684 552 320 309 182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3249 3258 487
Size: 683 56 2023 9 5510
Fragments arranged by size:
5510 2023 683 56 9
BtsI GCAGTG_nn'
Cuts at: 2910 3897 5797 6591 6611 2910
Size: 987 1900 794 20 4580
Fragments arranged by size:
4580 1900 987 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8281
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4824 4859 529
Size: 35 4260 35 3951
Fragments arranged by size:
4260 3951 35 35
DraI TTT'AAA
Cuts at: 5585 5585
Size: 8281
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7288 809
Size: 507 5972 1802
Fragments arranged by size:
5972 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2112 2118 2295
Size: 54 1425 50 275 18 282 6 177
Cuts at: 2295 2329 3793 4800 4976 5022 5298 5469 5487
Size: 34 1464 1007 176 46 276 171 18
Cuts at: 5487 5728 6617 8246 8
Size: 241 889 1629 43
Fragments arranged by size:
1629 1464 1425 1007 889 282 276 275
241 177 176 171 54 50 46 43
34 18 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2112 2118 2329 5298 5728 1537
Size: 275 300 6 211 2969 430 4090
Fragments arranged by size:
4090 2969 430 300 275 211 6
EarI CTCTTCn'nnn_
Cuts at: 1353 2216 3835 5083 6211 1353
Size: 863 1619 1248 1128 3423
Fragments arranged by size:
3423 1619 1248 1128 863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2079 2544 6868 7242 7388 387
Size: 1064 138 490 465 4324 374 146 1280
Fragments arranged by size:
4324 1280 1064 490 465 374 146 138
EcoICRI GAG'CTC
Cuts at: 3157 3605 3871 5305 3157
Size: 448 266 1434 6133
Fragments arranged by size:
6133 1434 448 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2012 2715 2805 2958 2987 3326 3424
Size: 393 380 703 90 153 29 339 98
Cuts at: 3424 3467 4415 4467 4557 4578 5420 6344 6941
Size: 43 948 52 90 21 842 924 597
Cuts at: 6941 7728 7996 1239
Size: 787 268 1524
Fragments arranged by size:
1524 948 924 842 787 703 597 393
380 339 268 153 98 90 90 52
43 29 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2811 2811
Size: 8281
EcoO109I rG'GnC_Cy
Cuts at: 2363 3077 3093 3915 4854 4855 5266 2363
Size: 714 16 822 939 1 411 5378
Fragments arranged by size:
5378 939 822 714 411 16 1
EcoRI G'AATT_C
Cuts at: 4831 5590 5968 4831
Size: 759 378 7144
Fragments arranged by size:
7144 759 378
FspI TGC'GCA
Cuts at: 3713 4775 6787 3713
Size: 1062 2012 5207
Fragments arranged by size:
5207 2012 1062
HaeII r_GCGC'y
Cuts at: 1678 2035 2127 2412 2431 2549 2688 3153 4893
Size: 357 92 285 19 118 139 465 1740
Cuts at: 4893 5141 5387 5541 7428 1678
Size: 248 246 154 1887 2531
Fragments arranged by size:
2531 1887 1740 465 357 285 248 246
154 139 118 92 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2140 2172 2355 2387 2688
Size: 32 135 32 568 32 183 32 301
Cuts at: 2688 2720 4364 4396 5081 5113 6922 6954 6996
Size: 32 1644 32 685 32 1809 32 42
Cuts at: 6996 7028 1373
Size: 32 2626
Fragments arranged by size:
2626 1809 1644 685 568 301 183 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5625 5871 669
Size: 988 281 3687 246 3079
Fragments arranged by size:
3687 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3988 748
Size: 3240 5041
HpaI GTT'AAC
Cuts at: 5871 5871
Size: 8281
KasI G'GCGC_C
Cuts at: 2123 2408 2427 2545 3149 4889 5137 2123
Size: 285 19 118 604 1740 248 5267
Fragments arranged by size:
5267 1740 604 285 248 118 19
KpnI G_GTAC'C
Cuts at: 5606 5606
Size: 8281
MfeI C'AATT_G
Cuts at: 5880 5880
Size: 8281
MluI A'CGCG_T
Cuts at: 8157 8157
Size: 8281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7395 7579 1305
Size: 6090 184 2007
Fragments arranged by size:
6090 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3795 4802 4978 10
Size: 54 1425 343 1963 1007 176 3313
Fragments arranged by size:
3313 1963 1425 1007 343 176 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6239 6598 6757 518
Size: 648 5073 359 159 2042
Fragments arranged by size:
5073 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2117 2924 3051 3129 3493 3773 3887 5143
Size: 511 807 127 78 364 280 114 1256
Cuts at: 5143 5164 5176 5275 5416 5697 6374 7522 7767
Size: 21 12 99 141 281 677 1148 245
Cuts at: 7767 1606
Size: 2120
Fragments arranged by size:
2120 1256 1148 807 677 511 364 281
280 245 141 127 114 99 78 21
12
NaeI GCC'GGC
Cuts at: 2122 2559 2605 2866 2122
Size: 437 46 261 7537
Fragments arranged by size:
7537 437 261 46
NarI GG'CG_CC
Cuts at: 2124 2409 2428 2546 3150 4890 5138 2124
Size: 285 19 118 604 1740 248 5267
Fragments arranged by size:
5267 1740 604 285 248 118 19
NcoI C'CATG_G
Cuts at: 513 1065 3484 4488 513
Size: 552 2419 1004 4306
Fragments arranged by size:
4306 2419 1004 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8281
NgoMIV G'CCGG_C
Cuts at: 2120 2557 2603 2864 2120
Size: 437 46 261 7537
Fragments arranged by size:
7537 437 261 46
NheI G'CTAG_C
Cuts at: 1052 5543 1052
Size: 4491 3790
NotI GC'GGCC_GC
Cuts at: 2112 5728 2112
Size: 3616 4665
NspI r_CATG'y
Cuts at: 1132 1229 5639 7184 1132
Size: 97 4410 1545 2229
Fragments arranged by size:
4410 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2661 3911 5371 1348
Size: 1313 1250 1460 4258
Fragments arranged by size:
4258 1460 1313 1250
PciI A'CATG_T
Cuts at: 7180 7180
Size: 8281
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3304 3688 3700 5203 1108
Size: 2196 384 12 1503 4186
Fragments arranged by size:
4186 2196 1503 384 12
PfoI T'CCnGG_A
Cuts at: 2359 2851 3100 3681 2359
Size: 492 249 581 6959
Fragments arranged by size:
6959 581 492 249
PmeI GTTT'AAAC
Cuts at: 5585 5585
Size: 8281
PmlI CAC'GTG
Cuts at: 4869 4869
Size: 8281
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6350 6382 7863 7895 6350
Size: 32 1481 32 6736
Fragments arranged by size:
6736 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2363 3915 5266 2363
Size: 1552 1351 5378
Fragments arranged by size:
5378 1552 1351
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8281
PsiI TTA'TAA
Cuts at: 4614 5851 4614
Size: 1237 7044
PspOMI G'GGCC_C
Cuts at: 3092 4854 3092
Size: 1762 6519
PspXI vC'TCGA_Gb
Cuts at: 2075 2075
Size: 8281
PstI C_TGCA'G
Cuts at: 830 3632 3892 4362 5633 830
Size: 2802 260 470 1271 3478
Fragments arranged by size:
3478 2802 1271 470 260
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8281
PvuII CAG'CTG
Cuts at: 3493 3773 3887 5176 5275 5416 3493
Size: 280 114 1289 99 141 6358
Fragments arranged by size:
6358 1289 280 141 114 99
SacI G_AGCT'C
Cuts at: 3159 3607 3873 5307 3159
Size: 448 266 1434 6133
Fragments arranged by size:
6133 1434 448 266
SacII CC_GC'GG
Cuts at: 2118 2118
Size: 8281
SalI G'TCGA_C
Cuts at: 1936 5623 1936
Size: 3687 4594
SanDI GG'GwC_CC
Cuts at: 2363 3915 2363
Size: 1552 6729
SbfI CC_TGCA'GG
Cuts at: 5633 5633
Size: 8281
ScaI AGT'ACT
Cuts at: 6529 8241 6529
Size: 1712 6569
SexAI A'CCwGG_T
Cuts at: 3297 3297
Size: 8281
SfcI C'TryA_G
Cuts at: 826 1045 2748 3628 3888 4358 5629 6764 7445
Size: 219 1703 880 260 470 1271 1135 681
Cuts at: 7445 7636 826
Size: 191 1471
Fragments arranged by size:
1703 1471 1271 1135 880 681 470 260
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2303 2303
Size: 8281
SfoI GGC'GCC
Cuts at: 2125 2410 2429 2547 3151 4891 5139 2125
Size: 285 19 118 604 1740 248 5267
Fragments arranged by size:
5267 1740 604 285 248 118 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8281
SgrAI Cr'CCGG_yG
Cuts at: 1700 2129 1700
Size: 429 7852
SmaI CCC'GGG
Cuts at: 1872 2944 4212 5451 5608 1872
Size: 1072 1268 1239 157 4545
Fragments arranged by size:
4545 1268 1239 1072 157
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2075 2537 3038 6385 7286 7548
Size: 197 926 132 462 501 3347 901 262
Cuts at: 7548 820
Size: 1553
Fragments arranged by size:
3347 1553 926 901 501 462 262 197
132
SnaBI TAC'GTA
Cuts at: 493 6072 493
Size: 5579 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8281
SphI G_CATG'C
Cuts at: 1132 5639 1132
Size: 4507 3774
SspI AAT'ATT
Cuts at: 5 52 4609 6205 5
Size: 47 4557 1596 2081
Fragments arranged by size:
4557 2081 1596 47
StuI AGG'CCT
Cuts at: 1517 3525 4308 1517
Size: 2008 783 5490
Fragments arranged by size:
5490 2008 783
StyI C'CwwG_G
Cuts at: 513 1065 2829 3484 3531 4488 4824 5720 513
Size: 552 1764 655 47 957 336 896 3074
Fragments arranged by size:
3074 1764 957 896 655 552 336 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2692 3221 4289 6308 6325 6478 6663
Size: 1255 1112 529 1068 2019 17 153 185
Cuts at: 6663 7108 325
Size: 445 1498
Fragments arranged by size:
2019 1498 1255 1112 1068 529 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3327 6527 8239
Size: 274 80 33 51 1443 1350 3200 1712
Cuts at: 8239 96
Size: 138
Fragments arranged by size:
3200 1712 1443 1350 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2216 2248 8241 8273 2216
Size: 32 5993 32 2224
Fragments arranged by size:
5993 2224 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8281
XbaI T'CTAG_A
Cuts at: 5617 5617
Size: 8281
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3142 4882 3142
Size: 1740 6541
XhoI C'TCGA_G
Cuts at: 1943 2075 1943
Size: 132 8149
XmaI C'CCGG_G
Cuts at: 1870 2942 4210 5449 5606 1870
Size: 1072 1268 1239 157 4545
Fragments arranged by size:
4545 1268 1239 1072 157
XmnI GAAnn'nnTTC
Cuts at: 3461 6410 3461
Size: 2949 5332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6089 276
Size: 53 83 186 936 4555 2468
Fragments arranged by size:
4555 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstXI
BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PsrI RsrII SapI SrfI SwaI