Restriction map for
pFN21AE2738
(Linear) (Six-base) MAPSORT of: KIEE2738.seq Check: 4976 from: 1 to: 3576
KIEE2738.orf
With 182 enzymes: SgfI *
December 20, 2018 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2393 3576
Size: 2393 1183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 1419 2410 2552 2573 3576
Size: 7 1412 991 142 21 1003
Fragments arranged by size:
1412 1003 991 142 21 7
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1759 3576
Size: 1759 1817
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 191 223 3576
Size: 191 32 3353
Fragments arranged by size:
3353 191 32
AlwNI CAG_nnn'CTG
Cuts at: 0 907 1299 1420 1725 1891 3123 3576
Size: 907 392 121 305 166 1232 453
Fragments arranged by size:
1232 907 453 392 305 166 121
ApaI G_GGCC'C
Cuts at: 0 1091 2853 3576
Size: 1091 1762 723
Fragments arranged by size:
1762 1091 723
ApoI r'AATT_y
Cuts at: 0 2403 2826 3576
Size: 2403 423 750
Fragments arranged by size:
2403 750 423
AvaI C'yCGr_G
Cuts at: 0 70 164 441 785 937 1571 2205 2220
Size: 70 94 277 344 152 634 634 15
Cuts at: 2220 2411 3444 3576
Size: 191 1033 132
Fragments arranged by size:
1033 634 634 344 277 191 152 132
94 70 15
BamHI G'GATC_C
Cuts at: 0 150 3576
Size: 150 3426
BanI G'GyrC_C
Cuts at: 0 78 118 127 250 403 422 514 540
Size: 78 40 9 123 153 19 92 26
Cuts at: 540 1144 1386 2884 3132 3576
Size: 604 242 1498 248 444
Fragments arranged by size:
1498 604 444 248 242 153 123 92
78 40 26 19 9
BanII G_rGCy'C
Cuts at: 0 269 385 464 742 786 1091 1154 1602
Size: 269 116 79 278 44 305 63 448
Cuts at: 1602 1658 1868 2267 2418 2455 2853 3302 3576
Size: 56 210 399 151 37 398 449 274
Fragments arranged by size:
449 448 399 398 305 278 274 269
210 151 116 79 63 56 44 37
BbeI G_GCGC'C
Cuts at: 0 122 407 426 544 1148 2888 3136 3576
Size: 122 285 19 118 604 1740 248 440
Fragments arranged by size:
1740 604 440 285 248 122 118 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 1106 1312 1425 1665 3576
Size: 1106 206 113 240 1911
Fragments arranged by size:
1911 1106 240 206 113
BbvCI CC'TCA_GC
Cuts at: 0 2247 2419 3576
Size: 2247 172 1157
Fragments arranged by size:
2247 1157 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1864 1898 3576
Size: 1864 34 1678
Fragments arranged by size:
1864 1678 34
BclI T'GATC_A
Cuts at: 0 1175 3576
Size: 1175 2401
BglI GCCn_nnn'nGGC
Cuts at: 0 167 298 476 939 2803 3187 3397 3576
Size: 167 131 178 463 1864 384 210 179
Fragments arranged by size:
1864 463 384 210 179 178 167 131
BlpI GC'TnA_GC
Cuts at: 0 433 3139 3576
Size: 433 2706 437
Fragments arranged by size:
2706 437 433
Bme1580I G_kGCm'C
Cuts at: 0 253 1091 1389 2853 2932 3576
Size: 253 838 298 1464 79 644
Fragments arranged by size:
1464 838 644 298 253 79
BmgBI CAC'GTC
Cuts at: 0 2660 3198 3309 3348 3576
Size: 2660 538 111 39 228
Fragments arranged by size:
2660 538 228 111 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 155 301 2129 2339 3576
Size: 155 146 1828 210 1237
Fragments arranged by size:
1828 1237 210 155 146
BmtI G_CTAG'C
Cuts at: 0 3542 3576
Size: 3542 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 800 953 982 1321 1462 2462 3415
Size: 710 90 153 29 339 141 1000 953
Cuts at: 3415 3576
Size: 161
Fragments arranged by size:
1000 953 710 339 161 153 141 90
29
Bpu10I CC'TnA_GC
Cuts at: 0 2247 2419 2449 3576
Size: 2247 172 30 1127
Fragments arranged by size:
2247 1127 172 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1018 3576
Size: 517 501 2558
Fragments arranged by size:
2558 517 501
BsaI GGTCTCn'nnnn_
Cuts at: 0 705 2438 3576
Size: 705 1733 1138
Fragments arranged by size:
1733 1138 705
BsaAI yAC'GTr
Cuts at: 0 2864 3576
Size: 2864 712
BsaHI Gr'CG_yC
Cuts at: 0 119 404 423 541 1145 2885 3133 3362
Size: 119 285 19 118 604 1740 248 229
Cuts at: 3362 3557 3576
Size: 195 19
Fragments arranged by size:
1740 604 285 248 229 195 119 118
19 19
BsaWI w'CCGG_w
Cuts at: 0 1779 2399 2810 3576
Size: 1779 620 411 766
Fragments arranged by size:
1779 766 620 411
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 683 713 3076 3106 3576
Size: 683 30 2363 30 470
Fragments arranged by size:
2363 683 470 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 405 536 857 860 1529 2514 2701
Size: 190 215 131 321 3 669 985 187
Cuts at: 2701 3097 3166 3576
Size: 396 69 410
Fragments arranged by size:
985 669 410 396 321 215 190 187
131 69 3
BseYI C'CCAG_C
Cuts at: 0 270 446 772 1105 1140 1233 1381 1409
Size: 270 176 326 333 35 93 148 28
Cuts at: 1409 1747 2338 2542 3159 3475 3493 3511 3576
Size: 338 591 204 617 316 18 18 65
Fragments arranged by size:
617 591 338 333 326 316 270 204
176 148 93 65 35 28 18 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1900 3205 3576
Size: 929 971 1305 371
Fragments arranged by size:
1305 971 929 371
BsiEI CG_ry'CG
Cuts at: 0 110 116 327 3296 3576
Size: 110 6 211 2969 280
Fragments arranged by size:
2969 280 211 110 6
BsiHKAI G_wGCw'C
Cuts at: 0 1066 1154 1602 1868 1903 2284 3145 3302
Size: 1066 88 448 266 35 381 861 157
Cuts at: 3302 3576
Size: 274
Fragments arranged by size:
1066 861 448 381 274 266 157 88
35
BsmBI CGTCTCn'nnnn_
Cuts at: 0 208 1480 3576
Size: 208 1272 2096
Fragments arranged by size:
2096 1272 208
Bsp1286I G_dGCh'C
Cuts at: 0 253 269 385 464 742 786 1066 1091
Size: 253 16 116 79 278 44 280 25
Cuts at: 1091 1154 1389 1602 1658 1868 1903 2267 2284
Size: 63 235 213 56 210 35 364 17
Cuts at: 2284 2418 2455 2853 2932 3145 3302 3576
Size: 134 37 398 79 213 157 274
Fragments arranged by size:
398 364 280 278 274 253 235 213
213 210 157 134 116 79 79 63
56 44 37 35 25 17 16
BspEI T'CCGG_A
Cuts at: 0 2810 3576
Size: 2810 766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2393 3576
Size: 2393 1183
BsrBI CCG'CTC
Cuts at: 0 319 366 1205 2691 3243 3576
Size: 319 47 839 1486 552 333
Fragments arranged by size:
1486 839 552 333 319 47
BsrDI GCAATG_nn'
Cuts at: 0 2746 3576
Size: 2746 830
BsrFI r'CCGG_y
Cuts at: 0 115 124 212 517 552 598 859 3576
Size: 115 9 88 305 35 46 261 2717
Fragments arranged by size:
2717 305 261 115 88 46 35 9
BssHII G'CGCG_C
Cuts at: 0 17 3576
Size: 17 3559
BssSI C'ACGA_G
Cuts at: 0 237 582 1016 1589 3576
Size: 237 345 434 573 1987
Fragments arranged by size:
1987 573 434 345 237
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1393 3327 3576
Size: 1393 1934 249
Fragments arranged by size:
1934 1393 249
BstEII G'GTnAC_C
Cuts at: 0 1698 1855 3576
Size: 1698 157 1721
Fragments arranged by size:
1721 1698 157
BstYI r'GATC_y
Cuts at: 0 150 2723 3576
Size: 150 2573 853
Fragments arranged by size:
2573 853 150
Bsu36I CC'TnA_GG
Cuts at: 0 1166 3576
Size: 1166 2410
BtgI C'CryG_G
Cuts at: 0 110 1479 2163 2483 2792 2974 3576
Size: 110 1369 684 320 309 182 602
Fragments arranged by size:
1369 684 602 320 309 182 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1244 1253 3576
Size: 1244 9 2323
Fragments arranged by size:
2323 1244 9
BtsI GCAGTG_nn'
Cuts at: 0 905 1892 3576
Size: 905 987 1684
Fragments arranged by size:
1684 987 905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2819 2854 3576
Size: 2819 35 722
Fragments arranged by size:
2819 722 35
EaeI y'GGCC_r
Cuts at: 0 107 113 290 324 1788 2795 2971 3017
Size: 107 6 177 34 1464 1007 176 46
Cuts at: 3017 3293 3464 3482 3576
Size: 276 171 18 94
Fragments arranged by size:
1464 1007 276 177 176 171 107 94
46 34 18 6
EagI C'GGCC_G
Cuts at: 0 107 113 324 3293 3576
Size: 107 6 211 2969 283
Fragments arranged by size:
2969 283 211 107 6
EarI CTCTTCn'nnn_
Cuts at: 0 211 1830 3078 3576
Size: 211 1619 1248 498
Fragments arranged by size:
1619 1248 498 211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 74 539 3576
Size: 74 465 3037
Fragments arranged by size:
3037 465 74
EcoICRI GAG'CTC
Cuts at: 0 1152 1600 1866 3300 3576
Size: 1152 448 266 1434 276
Fragments arranged by size:
1434 1152 448 276 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 710 800 953 982 1321 1419 1462
Size: 7 703 90 153 29 339 98 43
Cuts at: 1462 2410 2462 2552 2573 3415 3576
Size: 948 52 90 21 842 161
Fragments arranged by size:
948 842 703 339 161 153 98 90
90 52 43 29 21 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 806 3576
Size: 806 2770
EcoO109I rG'GnC_Cy
Cuts at: 0 358 1072 1088 1910 2849 2850 3261 3576
Size: 358 714 16 822 939 1 411 315
Fragments arranged by size:
939 822 714 411 358 315 16 1
EcoRI G'AATT_C
Cuts at: 0 2826 3576
Size: 2826 750
FspI TGC'GCA
Cuts at: 0 1708 2770 3576
Size: 1708 1062 806
Fragments arranged by size:
1708 1062 806
HaeII r_GCGC'y
Cuts at: 0 30 122 407 426 544 683 1148 2888
Size: 30 92 285 19 118 139 465 1740
Cuts at: 2888 3136 3382 3536 3576
Size: 248 246 154 40
Fragments arranged by size:
1740 465 285 248 246 154 139 118
92 40 30 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 135 167 350 382 683 715 2359 2391
Size: 135 32 183 32 301 32 1644 32
Cuts at: 2391 3076 3108 3576
Size: 685 32 468
Fragments arranged by size:
1644 685 468 301 183 135 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1983 3576
Size: 1983 1593
KasI G'GCGC_C
Cuts at: 0 118 403 422 540 1144 2884 3132 3576
Size: 118 285 19 118 604 1740 248 444
Fragments arranged by size:
1740 604 444 285 248 118 118 19
MscI TGG'CCA
Cuts at: 0 1790 2797 2973 3576
Size: 1790 1007 176 603
Fragments arranged by size:
1790 1007 603 176
MspA1I CmG'CkG
Cuts at: 0 112 919 1046 1124 1488 1768 1882 3138
Size: 112 807 127 78 364 280 114 1256
Cuts at: 3138 3159 3171 3270 3411 3576
Size: 21 12 99 141 165
Fragments arranged by size:
1256 807 364 280 165 141 127 114
112 99 78 21 12
NaeI GCC'GGC
Cuts at: 0 117 554 600 861 3576
Size: 117 437 46 261 2715
Fragments arranged by size:
2715 437 261 117 46
NarI GG'CG_CC
Cuts at: 0 119 404 423 541 1145 2885 3133 3576
Size: 119 285 19 118 604 1740 248 443
Fragments arranged by size:
1740 604 443 285 248 119 118 19
NcoI C'CATG_G
Cuts at: 0 1479 2483 3576
Size: 1479 1004 1093
Fragments arranged by size:
1479 1093 1004
NgoMIV G'CCGG_C
Cuts at: 0 115 552 598 859 3576
Size: 115 437 46 261 2717
Fragments arranged by size:
2717 437 261 115 46
NheI G'CTAG_C
Cuts at: 0 3538 3576
Size: 3538 38
NotI GC'GGCC_GC
Cuts at: 0 107 3576
Size: 107 3469
PasI CC'CwG_GG
Cuts at: 0 656 1906 3366 3576
Size: 656 1250 1460 210
Fragments arranged by size:
1460 1250 656 210
PflMI CCAn_nnn'nTGG
Cuts at: 0 1299 1683 1695 3198 3576
Size: 1299 384 12 1503 378
Fragments arranged by size:
1503 1299 384 378 12
PfoI T'CCnGG_A
Cuts at: 0 354 846 1095 1676 3576
Size: 354 492 249 581 1900
Fragments arranged by size:
1900 581 492 354 249
PmlI CAC'GTG
Cuts at: 0 2864 3576
Size: 2864 712
PpuMI rG'GwC_Cy
Cuts at: 0 358 1910 3261 3576
Size: 358 1552 1351 315
Fragments arranged by size:
1552 1351 358 315
PsiI TTA'TAA
Cuts at: 0 2609 3576
Size: 2609 967
PspOMI G'GGCC_C
Cuts at: 0 1087 2849 3576
Size: 1087 1762 727
Fragments arranged by size:
1762 1087 727
PspXI vC'TCGA_Gb
Cuts at: 0 70 3576
Size: 70 3506
PstI C_TGCA'G
Cuts at: 0 1627 1887 2357 3576
Size: 1627 260 470 1219
Fragments arranged by size:
1627 1219 470 260
PvuII CAG'CTG
Cuts at: 0 1488 1768 1882 3171 3270 3411 3576
Size: 1488 280 114 1289 99 141 165
Fragments arranged by size:
1488 1289 280 165 141 114 99
SacI G_AGCT'C
Cuts at: 0 1154 1602 1868 3302 3576
Size: 1154 448 266 1434 274
Fragments arranged by size:
1434 1154 448 274 266
SacII CC_GC'GG
Cuts at: 0 113 3576
Size: 113 3463
SanDI GG'GwC_CC
Cuts at: 0 358 1910 3576
Size: 358 1552 1666
Fragments arranged by size:
1666 1552 358
SexAI A'CCwGG_T
Cuts at: 0 1292 3576
Size: 1292 2284
SfcI C'TryA_G
Cuts at: 0 743 1623 1883 2353 3576
Size: 743 880 260 470 1223
Fragments arranged by size:
1223 880 743 470 260
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 298 3576
Size: 298 3278
SfoI GGC'GCC
Cuts at: 0 120 405 424 542 1146 2886 3134 3576
Size: 120 285 19 118 604 1740 248 442
Fragments arranged by size:
1740 604 442 285 248 120 118 19
SgrAI Cr'CCGG_yG
Cuts at: 0 124 3576
Size: 124 3452
SmaI CCC'GGG
Cuts at: 0 939 2207 3446 3576
Size: 939 1268 1239 130
Fragments arranged by size:
1268 1239 939 130
SmlI C'TyrA_G
Cuts at: 0 70 532 1033 3576
Size: 70 462 501 2543
Fragments arranged by size:
2543 501 462 70
SspI AAT'ATT
Cuts at: 0 2604 3576
Size: 2604 972
StuI AGG'CCT
Cuts at: 0 1520 2303 3576
Size: 1520 783 1273
Fragments arranged by size:
1520 1273 783
StyI C'CwwG_G
Cuts at: 0 824 1479 1526 2483 2819 3576
Size: 824 655 47 957 336 757
Fragments arranged by size:
957 824 757 655 336 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 687 1216 2284 3576
Size: 687 529 1068 1292
Fragments arranged by size:
1292 1068 687 529
TatI w'GTAC_w
Cuts at: 0 1322 3576
Size: 1322 2254
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 211 243 3576
Size: 211 32 3333
Fragments arranged by size:
3333 211 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1137 2877 3576
Size: 1137 1740 699
Fragments arranged by size:
1740 1137 699
XhoI C'TCGA_G
Cuts at: 0 70 3576
Size: 70 3506
XmaI C'CCGG_G
Cuts at: 0 937 2205 3444 3576
Size: 937 1268 1239 132
Fragments arranged by size:
1268 1239 937 132
XmnI GAAnn'nnTTC
Cuts at: 0 1456 3576
Size: 1456 2120
Enzymes that do cut:
AarI AcuI AlfI AloI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstEII BstYI Bsu36I
BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HindIII KasI MscI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI PasI PflMI PfoI PmlI PpuMI PsiI
PspOMI PspXI PstI PvuII SacI SacII SanDI SexAI
SfcI SfiI SfoI SgrAI SmaI SmlI SspI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaLI AscI AseI AsiSI AvrII BaeI
BciVI BfrBI BglII BplI BsaBI BsiWI BsmI BspHI
BsrGI BstBI BstXI BstZ17I ClaI DraI DraIII DrdI
EcoRV FalI FseI FspAI HincII HpaI KpnI MfeI
MluI MmeI MslI NdeI NruI NsiI NspI PacI
PciI PmeI PpiI PshAI PsrI PvuI RsrII SalI
SapI SbfI ScaI SgfI SnaBI SpeI SphI SrfI
SwaI Tth111I XbaI ZraI