################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflIII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI BanI BbeI BbrPI BglI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI KpnI MamI MfeI MluI MroI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 1792 CGCG CG^CG AcsI 1777 AAATTC [GA]^AATT[CT] AcyI 22 GGCGTC G[GA]^CG[CT]C AflII 1288 CTTAAG C^TTAAG ApoI 1777 AAATTC [GA]^AATT[CT] Asp700I 331 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 2114 TGCGCA TGC^GCA AvrII 1704 CCTAGG C^CTAGG BalI 1655 TGGCCA TGG^CCA BbsI 1531 GAAGAC GAAGAC(2/6) BclI 2031 TGATCA T^GATCA BfrI 1288 CTTAAG C^TTAAG BglII 992 AGATCT A^GATCT BlnI 1704 CCTAGG C^CTAGG BlpI 1697 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1697 GCTCAGC GC^T[ACGT]AGC BpuAI 1531 GAAGAC GAAGAC(2/6) BsaHI 22 GGCGTC G[GA]^CG[CT]C Bsp1407I 1865 TGTACA T^GTACA BsrFI 39 GCCGGT [GA]^CCGG[CT] BsrGI 1865 TGTACA T^GTACA BstEII 1243 GGTGACC G^GT[ACGT]ACC BstPI 1243 GGTGACC G^GT[ACGT]ACC BstUI 1792 CGCG CG^CG BstXI 959 CCAAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1697 GCTCAGC GC^T[ACGT]AGC Cfr10I 39 GCCGGT [GA]^CCGG[CT] EarI 175 CTCTTC CTCTTC(1/4) EcoO65I 1243 GGTGACC G^GT[ACGT]ACC FbaI 2031 TGATCA T^GATCA FspI 2114 TGCGCA TGC^GCA HgaI 23 GCGTC GACGC(5/10) Hin1I 22 GGCGTC G[GA]^CG[CT]C HincII 823 GTCAAC GT[CT]^[GA]AC HindII 823 GTCAAC GT[CT]^[GA]AC Ksp632I 175 CTCTTC CTCTTC(1/4) KspI 1791 CCGCGG CCGC^GG MluNI 1655 TGGCCA TGG^CCA MscI 1655 TGGCCA TGG^CCA MvnI 1792 CGCG CG^CG NspI 2276 ACATGC [GA]CATG^[CT] PpuMI 140 AGGTCCC [GA]G^G[AT]CC[CT] SacI 2062 GAGCTC GAGCT^C SacII 1791 CCGCGG CCGC^GG SpeI 184 ACTAGT A^CTAGT SspBI 1865 TGTACA T^GTACA XmnI 331 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Aor51HI 2444 AGCGCT AGC^GCT 2529 AGCGCT BamHI 1832 GGATCC G^GATCC 2099 GGATCC BciVI 2120 GTATCC GTATCC(6/5) 2336 GTATCC BsaI 955 GAGACC GGTCTC(1/5) 1490 GAGACC BsgI 505 CTGCAC GTGCAG(16/14) 1330 GTGCAG BspMI 1358 ACCTGC ACCTGC(4/8) 1910 ACCTGC BsrDI 1403 CATTGC GCAATG(2/0) 1743 CATTGC DraII 140 AGGTCCC [GA]G^G[ACGT]CC[CT] 725 AGGGCCT DsaI 1791 CCGCGG C^C[GA][CT]GG 2282 CCACGG EaeI 47 TGGCCG [CT]^GGCC[GA] 1655 TGGCCA Eco47III 2444 AGCGCT AGC^GCT 2529 AGCGCT EcoNI 483 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 681 CCTATCTGAGG EcoO109I 140 AGGTCCC [GA]G^G[ACGT]CC[CT] 725 AGGGCCT MaeII 821 ACGT A^CGT 2470 ACGT PflMI 246 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2406 CCAAAAATTGG PleI 363 GACTC GAGTC(4/5) 384 GAGTC SfaNI 1558 GATGC GCATC(5/9) 2107 GATGC SmaI 27 CCCGGG CCC^GGG 65 CCCGGG TfiI 421 GAATC G^A[AT]TC 762 GAATC Van91I 246 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2406 CCAAAAATTGG XmaCI 27 CCCGGG C^CCGGG 65 CCCGGG XmaI 27 CCCGGG C^CCGGG 65 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BanII BbvCI BbvI BcgI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII Eco81I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII