Restriction map for
pFN21AB8542
(Circular) (Six-base) MAPSORT of: pFN21AB8542.seq Check: 1212 from: 1 to: 7405
pFN21AB8542 7405 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5215 278
Size: 53 83 186 936 3679 2468
Fragments arranged by size:
3679 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4748 1937
Size: 2811 4594
Acc65I G'GTAC_C
Cuts at: 4726 4726
Size: 7405
AclI AA'CG_TT
Cuts at: 1563 5532 5905 1563
Size: 3969 373 3063
Fragments arranged by size:
3969 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3944 4256 4698 5468 6852 7120 1632
Size: 2312 312 442 770 1384 268 1917
Fragments arranged by size:
2312 1917 1384 770 442 312 268
AfeI AGC'GCT
Cuts at: 4451 4536 4451
Size: 85 7320
AflII C'TTAA_G
Cuts at: 820 1017 3293 820
Size: 197 2276 4932
Fragments arranged by size:
4932 2276 197
AflIII A'CryG_T
Cuts at: 1203 6304 7281 1203
Size: 5101 977 1327
Fragments arranged by size:
5101 1327 977
AgeI A'CCGG_T
Cuts at: 6236 6236
Size: 7405
AhdI GACnn_n'nnGTC
Cuts at: 6134 6134
Size: 7405
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7405
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4361 1184
Size: 3177 4228
AlwNI CAG_nnn'CTG
Cuts at: 2257 2530 2567 3124 3352 3423 4045 4229 5148
Size: 273 37 557 228 71 622 184 919
Cuts at: 5148 6720 2257
Size: 1572 2942
Fragments arranged by size:
2942 1572 919 622 557 273 228 184
71 37
ApaLI G'TGCA_C
Cuts at: 5462 6618 5462
Size: 1156 6249
ApoI r'AATT_y
Cuts at: 1493 3782 4714 4940 5092 7201 1493
Size: 2289 932 226 152 2109 1697
Fragments arranged by size:
2289 2109 1697 932 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5959 160
Size: 872 4927 1606
Fragments arranged by size:
4927 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7405
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2070 3243 3492 4730 1870
Size: 73 89 38 1173 249 1238 4545
Fragments arranged by size:
4545 1238 1173 249 89 73 38
AvrII C'CTAG_G
Cuts at: 3709 3709
Size: 7405
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3501 3534 3501
Size: 33 7372
BamHI G'GATC_C
Cuts at: 3837 4104 4735 5102 3837
Size: 267 631 367 6140
Fragments arranged by size:
6140 631 367 267
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4726 6181 618
Size: 325 217 627 2939 1455 1842
Fragments arranged by size:
2939 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2071 2365 2458 4071 4176 4724 1391
Size: 680 294 93 1613 105 548 4072
Fragments arranged by size:
4072 1613 680 548 294 105 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3543 928
Size: 971 1644 4790
Fragments arranged by size:
4790 1644 971
BbvCI CC'TCA_GC
Cuts at: 2637 3717 4662 2637
Size: 1080 945 5380
Fragments arranged by size:
5380 1080 945
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2260 2294 2867 2901 3164 3198 4595
Size: 34 323 34 573 34 263 34 1397
Cuts at: 4595 4629 5596 5630 1903
Size: 34 967 34 3678
Fragments arranged by size:
3678 1397 967 573 323 263 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4136 4352 5297 6507 4136
Size: 216 945 1210 5034
Fragments arranged by size:
5034 1210 945 216
BclI T'GATC_A
Cuts at: 1548 4036 1548
Size: 2488 4917
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6016 136
Size: 107 122 71 631 605 4344 1525
Fragments arranged by size:
4344 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2997 7400 2997
Size: 4403 3002
BlpI GC'TnA_GC
Cuts at: 3703 4822 3703
Size: 1119 6286
Bme1580I G_kGCm'C
Cuts at: 1790 2509 2818 5466 6622 1790
Size: 719 309 2648 1156 2573
Fragments arranged by size:
2648 2573 1156 719 309
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7405
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3433 5136 6094
Size: 416 53 83 399 24 2016 1703 958
Cuts at: 6094 442
Size: 1753
Fragments arranged by size:
2016 1753 1703 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2808 2993 3395 3407 4055 4085 4314 6065
Size: 1569 185 402 12 648 30 229 1751
Cuts at: 6065 1239
Size: 2579
Fragments arranged by size:
2579 1751 1569 648 402 229 185 30
12
Bpu10I CC'TnA_GC
Cuts at: 2637 3369 3717 4662 2637
Size: 732 348 945 5380
Fragments arranged by size:
5380 945 732 348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2407 3458 4112 5530 6395 6693 2407
Size: 1051 654 1418 865 298 3119
Fragments arranged by size:
3119 1418 1051 865 654 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2954 3489 6068 882
Size: 617 1455 535 2579 2219
Fragments arranged by size:
2579 2219 1455 617 535
BsaAI yAC'GTr
Cuts at: 493 1204 5196 493
Size: 711 3992 2702
Fragments arranged by size:
3992 2702 711
BsaBI GATnn'nnATC
Cuts at: 7399 7399
Size: 7405
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 5212 5594 6229
Size: 53 83 186 936 495 3184 382 635
Cuts at: 6229 275
Size: 1451
Fragments arranged by size:
3184 1451 936 635 495 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5838 6236 6510 6657 7068 5838
Size: 398 274 147 411 6175
Fragments arranged by size:
6175 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2168 2198 3064 3094 3200
Size: 30 165 30 765 30 866 30 106
Cuts at: 3200 3230 3380 3410 3605 3635 1178
Size: 30 150 30 195 30 4948
Fragments arranged by size:
4948 866 765 195 165 150 106 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2510 2681 2684 2687 2708 2711 2990 3251
Size: 1324 171 3 3 21 3 279 261
Cuts at: 3251 3404 1186
Size: 153 5187
Fragments arranged by size:
5187 1324 279 261 171 153 21 3
3 3
BseYI C'CCAG_C
Cuts at: 2226 2414 2622 2627 2760 3974 4311 6608 7104
Size: 188 208 5 133 1214 337 2297 496
Cuts at: 7104 2226
Size: 2527
Fragments arranged by size:
2527 2297 1214 496 337 208 188 133
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2495 3356 1163
Size: 573 759 861 5212
Fragments arranged by size:
5212 861 759 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4855 5616 6644 1540
Size: 275 186 2854 761 1028 2301
Fragments arranged by size:
2854 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 2805 2871 3177 3184 4026 4071 5466 5551
Size: 714 66 306 7 842 45 1395 85
Cuts at: 5551 6622 2091
Size: 1071 2874
Fragments arranged by size:
2874 1395 1071 842 714 306 85 66
45 7
BsmI GAATG_Cn'
Cuts at: 4916 5009 4916
Size: 93 7312
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2091 2365 2458 2509 2805 2818
Size: 399 281 20 274 93 51 296 13
Cuts at: 2818 2871 3177 3184 4026 4071 4176 4724 5466
Size: 53 306 7 842 45 105 548 742
Cuts at: 5466 5551 6622 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 842 742 548 399 306 296
281 274 105 93 85 53 51 45
20 13 7
BspEI T'CCGG_A
Cuts at: 7068 7068
Size: 7405
BspHI T'CATG_A
Cuts at: 5294 7024 5294
Size: 1730 5675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3372 3924 4760 844
Size: 2528 552 836 3489
Fragments arranged by size:
3489 2528 836 552
BsrBI CCG'CTC
Cuts at: 1394 1823 5292 1394
Size: 429 3469 3507
Fragments arranged by size:
3507 3469 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3407 3747 5900 6074 65
Size: 1183 2159 340 2153 174 1396
Fragments arranged by size:
2159 2153 1396 1183 340 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2044 6049 6236 1700
Size: 110 234 4005 187 2869
Fragments arranged by size:
4005 2869 234 187 110
BsrGI T'GTAC_A
Cuts at: 96 3870 96
Size: 3774 3631
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7405
BssSI C'ACGA_G
Cuts at: 5465 6477 5465
Size: 1012 6393
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7405
BstBI TT'CG_AA
Cuts at: 6209 6209
Size: 7405
BstEII G'GTnAC_C
Cuts at: 1184 3248 1184
Size: 2064 5341
BstXI CCAn_nnnn'nTGG
Cuts at: 2971 2971
Size: 7405
BstYI r'GATC_y
Cuts at: 1972 2476 2997 3837 4104 4636 4735 5102 5487
Size: 504 521 840 267 532 99 367 385
Cuts at: 5487 5504 6956 7042 7054 7400 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 840 532 521 504 385 367
346 267 99 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2142 2493 2664 3882 2142
Size: 351 171 1218 5665
Fragments arranged by size:
5665 1218 351 171
BtgI C'CryG_G
Cuts at: 513 1065 3796 4287 513
Size: 552 2731 491 3631
Fragments arranged by size:
3631 2731 552 491
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3181 487
Size: 683 56 1955 4711
Fragments arranged by size:
4711 1955 683 56
BtsI GCAGTG_nn'
Cuts at: 3424 4201 4245 4921 5715 5735 3424
Size: 777 44 676 794 20 5094
Fragments arranged by size:
5094 794 777 676 44 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7405
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7370
DraI TTT'AAA
Cuts at: 4709 4709
Size: 7405
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6412 809
Size: 507 5096 1802
Fragments arranged by size:
5096 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 3660 4852
Size: 54 1425 50 275 18 222 1608 1192
Cuts at: 4852 5741 7370 8
Size: 889 1629 43
Fragments arranged by size:
1629 1608 1425 1192 889 275 222 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4852 1537
Size: 275 3040 4090
Fragments arranged by size:
4090 3040 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 5335 1353
Size: 833 3149 3423
Fragments arranged by size:
3423 3149 833
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2894 5992 6366 6512 387
Size: 1064 138 1305 3098 374 146 1280
Fragments arranged by size:
3098 1305 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4069 4069
Size: 7405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2808 2993 3395 3407 3944 4055 4085
Size: 393 1176 185 402 12 537 111 30
Cuts at: 4085 4256 4314 4698 5468 6065 6852 7120 1239
Size: 171 58 384 770 597 787 268 1524
Fragments arranged by size:
1524 1176 787 770 597 537 402 393
384 268 185 171 111 58 30 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2492 2690 2492
Size: 198 7207
EcoO109I rG'GnC_Cy
Cuts at: 2146 2731 2146
Size: 585 6820
EcoRI G'AATT_C
Cuts at: 4714 5092 4714
Size: 378 7027
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2360 2360
Size: 7405
FspI TGC'GCA
Cuts at: 4121 5911 4121
Size: 1790 5615
HaeII r_GCGC'y
Cuts at: 1678 3859 4045 4453 4538 6552 1678
Size: 2181 186 408 85 2014 2531
Fragments arranged by size:
2531 2181 2014 408 186 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2411 2413 2443 2445 2953
Size: 32 135 32 839 2 30 2 508
Cuts at: 2953 2985 6046 6078 6120 6152 1373
Size: 32 3061 32 42 32 2626
Fragments arranged by size:
3061 2626 839 508 135 42 32 32
32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2830 4749 4995 669
Size: 988 281 892 1919 246 3079
Fragments arranged by size:
3079 1919 988 892 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7405
HpaI GTT'AAC
Cuts at: 4995 4995
Size: 7405
KpnI G_GTAC'C
Cuts at: 4730 4730
Size: 7405
MfeI C'AATT_G
Cuts at: 5004 5004
Size: 7405
MluI A'CGCG_T
Cuts at: 7281 7281
Size: 7405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6519 6703 1305
Size: 5214 184 2007
Fragments arranged by size:
5214 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3662 10
Size: 54 1425 343 1830 3753
Fragments arranged by size:
3753 1830 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5363 5722 5881 518
Size: 648 4197 359 159 2042
Fragments arranged by size:
4197 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2161 2517 2636 2752 3039 3798 4315 4821
Size: 555 356 119 116 287 759 517 506
Cuts at: 4821 5498 6646 6891 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 759 677 555 517 506 356
287 245 119 116
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6853
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7405
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7405
NotI GC'GGCC_GC
Cuts at: 4852 4852
Size: 7405
NspI r_CATG'y
Cuts at: 1132 1229 4285 4763 6308 1132
Size: 97 3056 478 1545 2229
Fragments arranged by size:
3056 2229 1545 478 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7405
PciI A'CATG_T
Cuts at: 6304 6304
Size: 7405
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 4417 1108
Size: 1149 2160 4096
Fragments arranged by size:
4096 2160 1149
PfoI T'CCnGG_A
Cuts at: 3905 4107 3905
Size: 202 7203
PmeI GTTT'AAAC
Cuts at: 4709 4709
Size: 7405
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5474 5506 6987 7019 5474
Size: 32 1481 32 5860
Fragments arranged by size:
5860 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2146 2146
Size: 7405
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7405
PsiI TTA'TAA
Cuts at: 4975 4975
Size: 7405
PstI C_TGCA'G
Cuts at: 830 2283 2563 4320 4340 4757 830
Size: 1453 280 1757 20 417 3478
Fragments arranged by size:
3478 1757 1453 417 280 20
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7405
PvuII CAG'CTG
Cuts at: 2161 2517 4315 2161
Size: 356 1798 5251
Fragments arranged by size:
5251 1798 356
SacI G_AGCT'C
Cuts at: 4071 4071
Size: 7405
SacII CC_GC'GG
Cuts at: 3799 3799
Size: 7405
SalI G'TCGA_C
Cuts at: 1936 4747 1936
Size: 2811 4594
SbfI CC_TGCA'GG
Cuts at: 4757 4757
Size: 7405
ScaI AGT'ACT
Cuts at: 5653 7365 5653
Size: 1712 5693
SfcI C'TryA_G
Cuts at: 826 1045 2100 2279 2559 3753 4316 4336 4656
Size: 219 1055 179 280 1194 563 20 320
Cuts at: 4656 4753 5888 6569 6760 826
Size: 97 1135 681 191 1471
Fragments arranged by size:
1471 1194 1135 1055 681 563 320 280
219 191 179 97 20
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7405
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7405
SmaI CCC'GGG
Cuts at: 1872 2034 2072 4732 1872
Size: 162 38 2660 4545
Fragments arranged by size:
4545 2660 162 38
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2386 3293 3473 4091 5509 6410
Size: 197 926 443 907 180 618 1418 901
Cuts at: 6410 6672 820
Size: 262 1553
Fragments arranged by size:
1553 1418 926 907 901 618 443 262
197 180
SnaBI TAC'GTA
Cuts at: 493 5196 493
Size: 4703 2702
SpeI A'CTAG_T
Cuts at: 152 2189 152
Size: 2037 5368
SphI G_CATG'C
Cuts at: 1132 4763 1132
Size: 3631 3774
SspI AAT'ATT
Cuts at: 5 52 5329 5
Size: 47 5277 2081
Fragments arranged by size:
5277 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7405
StyI C'CwwG_G
Cuts at: 513 1065 2320 2845 2964 3709 3732 4844 513
Size: 552 1255 525 119 745 23 1112 3074
Fragments arranged by size:
3074 1255 1112 745 552 525 119 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3116 3445 5432 5449 5602 5787 6232
Size: 1255 1536 329 1987 17 153 185 445
Cuts at: 6232 325
Size: 1498
Fragments arranged by size:
1987 1536 1498 1255 445 329 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2304 2569 3870
Size: 274 80 33 51 1443 327 265 1301
Cuts at: 3870 5651 7363 96
Size: 1781 1712 138
Fragments arranged by size:
1781 1712 1443 1301 327 274 265 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3064 3096 3200 3232 3605 3637 4384 4416 7365
Size: 32 104 32 373 32 747 32 2949
Cuts at: 7365 7397 3064
Size: 32 3072
Fragments arranged by size:
3072 2949 747 373 104 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7405
XbaI T'CTAG_A
Cuts at: 4741 4741
Size: 7405
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7405
XmaI C'CCGG_G
Cuts at: 1870 2032 2070 4730 1870
Size: 162 38 2660 4545
Fragments arranged by size:
4545 2660 162 38
XmnI GAAnn'nnTTC
Cuts at: 2340 5534 2340
Size: 3194 4211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5213 276
Size: 53 83 186 936 3679 2468
Fragments arranged by size:
3679 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI