(Linear) (Six-base) MAPSORT of: KIBB8542.seq Check: 5115 from: 1 to: 2700 KIBB8542.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1939 2251 2693 2700 Size: 1939 312 442 7 Fragments arranged by size: 1939 442 312 7 AfeI AGC'GCT Cuts at: 0 2446 2531 2700 Size: 2446 85 169 Fragments arranged by size: 2446 169 85 AflII C'TTAA_G Cuts at: 0 1288 2700 Size: 1288 1412 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2356 2700 Size: 2356 344 AlwNI CAG_nnn'CTG Cuts at: 0 252 525 562 1119 1347 1418 2040 2224 Size: 252 273 37 557 228 71 622 184 Cuts at: 2224 2700 Size: 476 Fragments arranged by size: 622 557 476 273 252 228 184 71 37 ApoI r'AATT_y Cuts at: 0 1777 2700 Size: 1777 923 AvaI C'yCGr_G Cuts at: 0 27 65 1238 1487 2700 Size: 27 38 1173 249 1213 Fragments arranged by size: 1213 1173 249 38 27 AvrII C'CTAG_G Cuts at: 0 1704 2700 Size: 1704 996 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1496 1529 2700 Size: 1496 33 1171 Fragments arranged by size: 1496 1171 33 BamHI G'GATC_C Cuts at: 0 1832 2099 2700 Size: 1832 267 601 Fragments arranged by size: 1832 601 267 BanII G_rGCy'C Cuts at: 0 66 360 453 2066 2171 2700 Size: 66 294 93 1613 105 529 Fragments arranged by size: 1613 529 294 105 93 66 BbsI GAAGACnn'nnnn_ Cuts at: 0 1538 2700 Size: 1538 1162 BbvCI CC'TCA_GC Cuts at: 0 632 1712 2657 2700 Size: 632 1080 945 43 Fragments arranged by size: 1080 945 632 43 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 255 289 862 896 1159 1193 2590 2624 Size: 255 34 573 34 263 34 1397 34 Cuts at: 2624 2700 Size: 76 Fragments arranged by size: 1397 573 263 255 76 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2131 2347 2700 Size: 2131 216 353 Fragments arranged by size: 2131 353 216 BclI T'GATC_A Cuts at: 0 2031 2700 Size: 2031 669 BglII A'GATC_T Cuts at: 0 992 2700 Size: 992 1708 BlpI GC'TnA_GC Cuts at: 0 1698 2700 Size: 1698 1002 Bme1580I G_kGCm'C Cuts at: 0 504 813 2700 Size: 504 309 1887 Fragments arranged by size: 1887 504 309 BmrI ACTGGGnnnn_n' Cuts at: 0 1428 2700 Size: 1428 1272 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 803 988 1390 1402 2050 2080 2309 2700 Size: 803 185 402 12 648 30 229 391 Fragments arranged by size: 803 648 402 391 229 185 30 12 Bpu10I CC'TnA_GC Cuts at: 0 632 1364 1712 2657 2700 Size: 632 732 348 945 43 Fragments arranged by size: 945 732 632 348 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 402 1453 2107 2700 Size: 402 1051 654 593 Fragments arranged by size: 1051 654 593 402 BsaI GGTCTCn'nnnn_ Cuts at: 0 949 1484 2700 Size: 949 535 1216 Fragments arranged by size: 1216 949 535 BsaHI Gr'CG_yC Cuts at: 0 23 2700 Size: 23 2677 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 163 193 1059 1089 1195 1225 1375 1405 Size: 163 30 866 30 106 30 150 30 Cuts at: 1405 1600 1630 2700 Size: 195 30 1070 Fragments arranged by size: 1070 866 195 163 150 106 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 505 676 679 682 703 706 985 1246 Size: 505 171 3 3 21 3 279 261 Cuts at: 1246 1399 2700 Size: 153 1301 Fragments arranged by size: 1301 505 279 261 171 153 21 3 3 3 BseYI C'CCAG_C Cuts at: 0 221 409 617 622 755 1969 2306 2700 Size: 221 188 208 5 133 1214 337 394 Fragments arranged by size: 1214 394 337 221 208 188 133 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 490 1351 2700 Size: 490 861 1349 Fragments arranged by size: 1349 861 490 BsiHKAI G_wGCw'C Cuts at: 0 86 800 866 1172 1179 2021 2066 2700 Size: 86 714 66 306 7 842 45 634 Fragments arranged by size: 842 714 634 306 86 66 45 7 Bsp1286I G_dGCh'C Cuts at: 0 66 86 360 453 504 800 813 866 Size: 66 20 274 93 51 296 13 53 Cuts at: 866 1172 1179 2021 2066 2171 2700 Size: 306 7 842 45 105 529 Fragments arranged by size: 842 529 306 296 274 105 93 66 53 51 45 20 13 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1367 1919 2700 Size: 1367 552 781 Fragments arranged by size: 1367 781 552 BsrDI GCAATG_nn' Cuts at: 0 1402 1742 2700 Size: 1402 340 958 Fragments arranged by size: 1402 958 340 BsrFI r'CCGG_y Cuts at: 0 39 2700 Size: 39 2661 BsrGI T'GTAC_A Cuts at: 0 1865 2700 Size: 1865 835 BstEII G'GTnAC_C Cuts at: 0 1243 2700 Size: 1243 1457 BstXI CCAn_nnnn'nTGG Cuts at: 0 966 2700 Size: 966 1734 BstYI r'GATC_y Cuts at: 0 471 992 1832 2099 2631 2700 Size: 471 521 840 267 532 69 Fragments arranged by size: 840 532 521 471 267 69 Bsu36I CC'TnA_GG Cuts at: 0 137 488 659 1877 2700 Size: 137 351 171 1218 823 Fragments arranged by size: 1218 823 351 171 137 BtgI C'CryG_G Cuts at: 0 1791 2282 2700 Size: 1791 491 418 Fragments arranged by size: 1791 491 418 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1176 2700 Size: 1176 1524 BtsI GCAGTG_nn' Cuts at: 0 1419 2196 2240 2700 Size: 1419 777 44 460 Fragments arranged by size: 1419 777 460 44 EaeI y'GGCC_r Cuts at: 0 47 1655 2700 Size: 47 1608 1045 Fragments arranged by size: 1608 1045 47 EarI CTCTTCn'nnn_ Cuts at: 0 181 2700 Size: 181 2519 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 889 2700 Size: 889 1811 EcoICRI GAG'CTC Cuts at: 0 2064 2700 Size: 2064 636 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 803 988 1390 1402 1939 2050 2080 2251 Size: 803 185 402 12 537 111 30 171 Cuts at: 2251 2309 2693 2700 Size: 58 384 7 Fragments arranged by size: 803 537 402 384 185 171 111 58 30 12 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 487 685 2700 Size: 487 198 2015 Fragments arranged by size: 2015 487 198 EcoO109I rG'GnC_Cy Cuts at: 0 141 726 2700 Size: 141 585 1974 Fragments arranged by size: 1974 585 141 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 355 2700 Size: 355 2345 FspI TGC'GCA Cuts at: 0 2116 2700 Size: 2116 584 HaeII r_GCGC'y Cuts at: 0 1854 2040 2448 2533 2700 Size: 1854 186 408 85 167 Fragments arranged by size: 1854 408 186 167 85 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 406 408 438 440 948 980 2700 Size: 406 2 30 2 508 32 1720 Fragments arranged by size: 1720 508 406 32 30 2 2 HincII GTy'rAC Cuts at: 0 825 2700 Size: 825 1875 MscI TGG'CCA Cuts at: 0 1657 2700 Size: 1657 1043 MspA1I CmG'CkG Cuts at: 0 156 512 631 747 1034 1793 2310 2700 Size: 156 356 119 116 287 759 517 390 Fragments arranged by size: 759 517 390 356 287 156 119 116 NspI r_CATG'y Cuts at: 0 2280 2700 Size: 2280 420 PflMI CCAn_nnn'nTGG Cuts at: 0 252 2412 2700 Size: 252 2160 288 Fragments arranged by size: 2160 288 252 PfoI T'CCnGG_A Cuts at: 0 1900 2102 2700 Size: 1900 202 598 Fragments arranged by size: 1900 598 202 PpuMI rG'GwC_Cy Cuts at: 0 141 2700 Size: 141 2559 PstI C_TGCA'G Cuts at: 0 278 558 2315 2335 2700 Size: 278 280 1757 20 365 Fragments arranged by size: 1757 365 280 278 20 PvuII CAG'CTG Cuts at: 0 156 512 2310 2700 Size: 156 356 1798 390 Fragments arranged by size: 1798 390 356 156 SacI G_AGCT'C Cuts at: 0 2066 2700 Size: 2066 634 SacII CC_GC'GG Cuts at: 0 1794 2700 Size: 1794 906 SfcI C'TryA_G Cuts at: 0 95 274 554 1748 2311 2331 2651 2700 Size: 95 179 280 1194 563 20 320 49 Fragments arranged by size: 1194 563 320 280 179 95 49 20 SmaI CCC'GGG Cuts at: 0 29 67 2700 Size: 29 38 2633 Fragments arranged by size: 2633 38 29 SmlI C'TyrA_G Cuts at: 0 381 1288 1468 2086 2700 Size: 381 907 180 618 614 Fragments arranged by size: 907 618 614 381 180 SpeI A'CTAG_T Cuts at: 0 184 2700 Size: 184 2516 StyI C'CwwG_G Cuts at: 0 315 840 959 1704 1727 2700 Size: 315 525 119 745 23 973 Fragments arranged by size: 973 745 525 315 119 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1111 1440 2700 Size: 1111 329 1260 Fragments arranged by size: 1260 1111 329 TatI w'GTAC_w Cuts at: 0 299 564 1865 2700 Size: 299 265 1301 835 Fragments arranged by size: 1301 835 299 265 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1059 1091 1195 1227 1600 1632 2379 2411 Size: 1059 32 104 32 373 32 747 32 Cuts at: 2411 2700 Size: 289 Fragments arranged by size: 1059 747 373 289 104 32 32 32 32 XmaI C'CCGG_G Cuts at: 0 27 65 2700 Size: 27 38 2635 Fragments arranged by size: 2635 38 27 XmnI GAAnn'nnTTC Cuts at: 0 335 2700 Size: 335 2365 Enzymes that do cut: AcuI AfeI AflII AlfI AlwNI ApoI AvaI AvrII BaeI BamHI BanII BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII MscI MspA1I NspI PflMI PfoI PpuMI PstI PvuII SacI SacII SfcI SmaI SmlI SpeI StyI TaqII TatI TstI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI BanI BbeI BfrBI BglI BmgBI BmtI BplI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII BssSI BstAPI BstBI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI ZraI