Restriction map for
pFN21AE2734
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
Asp700I AspEI AviII AvrII BbeI
BbrPI BbvCI BcgI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII CfoI ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tsp509I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 911 GGTACC G^GTACC
AflIII 470 ACATGT A^C[GA][CT]GT
AlwNI 151 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 679 CCTCAGG CC^T[ACGT]AGG
ApaI 308 GGGCCC GGGCC^C
AseI 388 ATTAAT AT^TAAT
AsnI 388 ATTAAT AT^TAAT
Asp718I 911 GGTACC G^GTACC
AspHI 565 GAGCTC G[AT]GC[AT]^C
AspI 931 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 346 GGTCC G^G[AT]CC
BalI 122 TGGCCA TGG^CCA
BamHI 181 GGATCC G^GATCC
BanI 911 GGTACC G^G[CT][GA]CC
BbsI 82 GTCTTC GAAGAC(2/6)
BciVI 201 GGATAC GTATCC(6/5)
BpmI 643 CTGGAG CTGGAG(16/14)
BpuAI 82 GTCTTC GAAGAC(2/6)
BsaI 685 GAGACC GGTCTC(1/5)
BsiHKAI 565 GAGCTC G[AT]GC[AT]^C
BsmBI 519 CGTCTC CGTCTC(1/5)
BspLU11I 470 ACATGT A^CATGT
BsrFI 908 GCCGGT [GA]^CCGG[CT]
BstYI 181 GGATCC [GA]^GATC[CT]
Bsu36I 679 CCTCAGG CC^T[ACGT]AGG
Cfr10I 908 GCCGGT [GA]^CCGG[CT]
DraII 308 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 437 CCATGG C^C[GA][CT]GG
EaeI 122 TGGCCA [CT]^GGCC[GA]
Eco81I 679 CCTCAGG CC^T[ACGT]AGG
EcoO109I 308 GGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 198 GCGGG CCCGC(4/6)
HgaI 518 GCGTC GACGC(5/10)
HincII 466 GTCAAC GT[CT]^[GA]AC
HindII 466 GTCAAC GT[CT]^[GA]AC
HindIII 661 AAGCTT A^AGCTT
KpnI 911 GGTACC GGTAC^C
MaeII 80 ACGT A^CGT
MboII 83 TCTTC GAAGA(8/7)
MflI 181 GGATCC [GA]^GATC[CT]
MluNI 122 TGGCCA TGG^CCA
MscI 122 TGGCCA TGG^CCA
MwoI 293 GCGAACAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 437 CCATGG C^CATGG
PflFI 931 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 350 CCAACGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 388 ATTAAT AT^TAAT
PstI 217 CTGCAG CTGCA^G
PvuII 130 CAGCTG CAG^CTG
SacI 565 GAGCTC GAGCT^C
SexAI 398 ACCTGGT A^CC[AT]GGT
SfcI 217 CTGCAG C^T[GA][CT]AG
TaqI 413 TCGA T^CGA
Tth111I 931 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 413 TCGA T^CGA
Van91I 350 CCAACGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 181 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 78 GTAC GT^AC
912 GTAC
AlwI 182 GATCC GGATC(4/5)
181 GGATC
AvaI 452 CCCGGG C^[CT]CG[GA]G
689 CCCGGG
BseRI 853 CTCCTC GAGGAG(10/8)
175 GAGGAG
BsmAI 685 GAGAC GTCTC(1/5)
520 GTCTC
BsoBI 452 CCCGGG C^[CT]CG[GA]G
689 CCCGGG
BstXI 125 CCAAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
224 CCACCCTACTGG
MseI 389 TTAA T^TAA
736 TTAA
MslI 147 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
882 CACCCCTGTG
RsaI 78 GTAC GT^AC
912 GTAC
SmaI 452 CCCGGG CCC^GGG
689 CCCGGG
StuI 300 AGGCCT AGG^CCT
539 AGGCCT
Tru9I 389 TTAA T^TAA
736 TTAA
XmaCI 452 CCCGGG C^CCGGG
689 CCCGGG
XmaI 452 CCCGGG C^CCGGG
689 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MnlI MspA1I MspI
MvaI NciI NdeII NlaIII NlaIV
NspI PleI Sau3AI Sau96I ScrFI
SfaNI SphI StyI TseI Tsp45I
TspRI