(Circular) (Six-base) MAPSORT of: pFN21AE2734.seq Check: 5645 from: 1 to: 5644 pFN21AE2734 5644 bp With 182 enzymes: SgfI * September 25, 2018 16:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2146 2746 2146 Size: 600 5044 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3454 278 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2987 1937 Size: 1050 4594 Acc65I G'GTAC_C Cuts at: 2916 2965 2916 Size: 49 5595 AclI AA'CG_TT Cuts at: 1563 3771 4144 1563 Size: 2208 373 3063 Fragments arranged by size: 3063 2208 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2183 3707 5091 5359 1632 Size: 551 1524 1384 268 1917 Fragments arranged by size: 1917 1524 1384 551 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5447 AflIII A'CryG_T Cuts at: 1203 2475 4543 5520 1203 Size: 1272 2068 977 1327 Fragments arranged by size: 2068 1327 1272 977 AgeI A'CCGG_T Cuts at: 4475 4475 Size: 5644 AhdI GACnn_n'nnGTC Cuts at: 4373 4373 Size: 5644 AleI CACnn'nnGTG Cuts at: 1166 2891 1166 Size: 1725 3919 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2174 1184 Size: 990 4654 AlwNI CAG_nnn'CTG Cuts at: 2161 3387 4959 2161 Size: 1226 1572 2846 Fragments arranged by size: 2846 1572 1226 ApaI G_GGCC'C Cuts at: 2317 2317 Size: 5644 ApaLI G'TGCA_C Cuts at: 3701 4857 3701 Size: 1156 4488 ApoI r'AATT_y Cuts at: 1493 2953 3179 3331 5440 1493 Size: 1460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1460 226 152 AseI AT'TA_AT Cuts at: 160 1032 2394 4198 160 Size: 872 1362 1804 1606 Fragments arranged by size: 1804 1606 1362 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 AvaI C'yCGr_G Cuts at: 1870 1943 2457 2694 2969 1870 Size: 73 514 237 275 4545 Fragments arranged by size: 4545 514 275 237 73 BamHI G'GATC_C Cuts at: 2186 2974 3341 2186 Size: 788 367 4489 Fragments arranged by size: 4489 788 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2916 2965 4420 618 Size: 325 217 627 1129 49 1455 1842 Fragments arranged by size: 1842 1455 1129 627 325 217 49 BanII G_rGCy'C Cuts at: 1391 2317 2458 2574 2769 2822 2963 1391 Size: 926 141 116 195 53 141 4072 Fragments arranged by size: 4072 926 195 141 141 116 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2080 928 Size: 971 181 4492 Fragments arranged by size: 4492 971 181 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3835 3869 1903 Size: 34 1898 34 3678 Fragments arranged by size: 3678 1898 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2200 3536 4746 2200 Size: 1336 1210 3098 Fragments arranged by size: 3098 1336 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4255 136 Size: 107 122 71 631 605 337 2246 1525 Fragments arranged by size: 2246 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5639 5639 Size: 5644 BlpI GC'TnA_GC Cuts at: 3061 3061 Size: 5644 Bme1580I G_kGCm'C Cuts at: 1790 2317 3705 4861 1790 Size: 527 1388 1156 2573 Fragments arranged by size: 2573 1388 1156 527 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5644 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2269 2340 3375 Size: 416 53 83 399 24 852 71 1035 Cuts at: 3375 4333 442 Size: 958 1753 Fragments arranged by size: 1753 1035 958 852 416 399 83 71 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5644 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2566 2700 2566 Size: 134 5510 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2669 4304 1239 Size: 1430 1635 2579 Fragments arranged by size: 2579 1635 1430 Bpu10I CC'TnA_GC Cuts at: 2909 2909 Size: 5644 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3769 4634 4932 3769 Size: 865 298 4481 Fragments arranged by size: 4481 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2684 4307 882 Size: 617 1185 1623 2219 Fragments arranged by size: 2219 1623 1185 617 BsaAI yAC'GTr Cuts at: 493 1204 3435 493 Size: 711 2231 2702 Fragments arranged by size: 2702 2231 711 BsaBI GATnn'nnATC Cuts at: 5638 5638 Size: 5644 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3451 3833 4468 275 Size: 53 83 186 936 1918 382 635 1451 Fragments arranged by size: 1918 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 4475 4749 4896 5307 4077 Size: 398 274 147 411 4414 Fragments arranged by size: 4414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2510 2540 1178 Size: 30 165 30 1107 30 4282 Fragments arranged by size: 4282 1107 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 2849 1186 Size: 1009 654 3981 Fragments arranged by size: 3981 1009 654 BseYI C'CCAG_C Cuts at: 4847 5343 4847 Size: 496 5148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5071 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3094 3855 4883 1540 Size: 275 186 1093 761 1028 2301 Fragments arranged by size: 2301 1093 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2574 3705 3790 4861 2574 Size: 1131 85 1071 3357 Fragments arranged by size: 3357 1131 1071 85 BsmI GAATG_Cn' Cuts at: 3155 3248 3155 Size: 93 5551 BsmBI CGTCTCn'nnnn_ Cuts at: 2530 2530 Size: 5644 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2317 2458 2574 2769 2822 2963 3705 Size: 399 527 141 116 195 53 141 742 Cuts at: 3705 3790 4861 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 527 399 195 141 141 116 85 53 BspEI T'CCGG_A Cuts at: 5307 5307 Size: 5644 BspHI T'CATG_A Cuts at: 3533 5263 3533 Size: 1730 3914 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2130 2146 2746 2999 844 Size: 1286 16 600 253 3489 Fragments arranged by size: 3489 1286 600 253 16 BsrBI CCG'CTC Cuts at: 1394 1823 3531 1394 Size: 429 1708 3507 Fragments arranged by size: 3507 1708 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4139 4313 65 Size: 1183 2891 174 1396 Fragments arranged by size: 2891 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2913 4288 4475 1700 Size: 110 1103 1375 187 2869 Fragments arranged by size: 2869 1375 1103 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5644 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5644 BssSI C'ACGA_G Cuts at: 3704 4716 3704 Size: 1012 4632 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5644 BstBI TT'CG_AA Cuts at: 4448 4448 Size: 5644 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5644 BstXI CCAn_nnnn'nTGG Cuts at: 2137 2236 2137 Size: 99 5545 BstYI r'GATC_y Cuts at: 1972 2186 2974 3341 3726 3743 5195 5281 5293 Size: 214 788 367 385 17 1452 86 12 Cuts at: 5293 5639 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 788 385 367 346 214 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2685 2685 Size: 5644 BtgI C'CryG_G Cuts at: 513 1065 2004 2442 513 Size: 552 939 438 3715 Fragments arranged by size: 3715 939 552 438 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4905 Fragments arranged by size: 4905 683 56 BtsI GCAGTG_nn' Cuts at: 3160 3954 3974 3160 Size: 794 20 4830 Fragments arranged by size: 4830 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5644 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5609 DraI TTT'AAA Cuts at: 2948 2948 Size: 5644 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4651 809 Size: 507 3335 1802 Fragments arranged by size: 3335 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 3091 3980 Size: 54 1425 50 275 18 297 964 889 Cuts at: 3980 5609 8 Size: 1629 43 Fragments arranged by size: 1629 1425 964 889 297 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3091 1537 Size: 275 1279 4090 Fragments arranged by size: 4090 1279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3574 1353 Size: 2221 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4231 4605 4751 387 Size: 1064 138 2642 374 146 1280 Fragments arranged by size: 2642 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2572 2572 Size: 5644 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2669 3707 4304 5091 5359 1239 Size: 393 551 486 1038 597 787 268 1524 Fragments arranged by size: 1524 1038 787 597 551 486 393 268 EcoO109I rG'GnC_Cy Cuts at: 2314 2314 Size: 5644 EcoRI G'AATT_C Cuts at: 2953 3331 2953 Size: 378 5266 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2104 2395 2444 2104 Size: 291 49 5304 Fragments arranged by size: 5304 291 49 FspI TGC'GCA Cuts at: 4150 4150 Size: 5644 HaeII r_GCGC'y Cuts at: 1678 4791 1678 Size: 3113 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2769 2801 4285 Size: 32 135 32 599 32 566 32 1484 Cuts at: 4285 4317 4359 4391 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1484 599 566 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2473 2988 3234 669 Size: 988 281 535 515 246 3079 Fragments arranged by size: 3079 988 535 515 281 246 HindIII A'AGCT_T Cuts at: 748 2666 748 Size: 1918 3726 HpaI GTT'AAC Cuts at: 3234 3234 Size: 5644 KpnI G_GTAC'C Cuts at: 2920 2969 2920 Size: 49 5595 MfeI C'AATT_G Cuts at: 3243 3243 Size: 5644 MluI A'CGCG_T Cuts at: 5520 5520 Size: 5644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4758 4942 1305 Size: 3453 184 2007 Fragments arranged by size: 3453 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2129 10 Size: 54 1425 343 297 3525 Fragments arranged by size: 3525 1425 343 297 54 MslI CAynn'nnrTG Cuts at: 518 1166 2156 2891 3602 3961 4120 518 Size: 648 990 735 711 359 159 2042 Fragments arranged by size: 2042 990 735 711 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2203 2933 3060 3737 4885 5130 1606 Size: 531 66 730 127 677 1148 245 2120 Fragments arranged by size: 2120 1148 730 677 531 245 127 66 NcoI C'CATG_G Cuts at: 513 1065 2004 2442 513 Size: 552 939 438 3715 Fragments arranged by size: 3715 939 552 438 NdeI CA'TA_TG Cuts at: 387 387 Size: 5644 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5644 NotI GC'GGCC_GC Cuts at: 3091 3091 Size: 5644 NspI r_CATG'y Cuts at: 1132 1229 2155 2437 2479 2662 2804 3002 4547 Size: 97 926 282 42 183 142 198 1545 Cuts at: 4547 1132 Size: 2229 Fragments arranged by size: 2229 1545 926 282 198 183 142 97 42 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5644 PciI A'CATG_T Cuts at: 2475 4543 2475 Size: 2068 3576 PflMI CCAn_nnn'nTGG Cuts at: 1108 2361 1108 Size: 1253 4391 PfoI T'CCnGG_A Cuts at: 2813 2813 Size: 5644 PmeI GTTT'AAAC Cuts at: 2948 2948 Size: 5644 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2292 2324 3713 3745 5226 5258 2292 Size: 32 1389 32 1481 32 2678 Fragments arranged by size: 2678 1481 1389 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5644 PsiI TTA'TAA Cuts at: 3214 3214 Size: 5644 PspOMI G'GGCC_C Cuts at: 2313 2313 Size: 5644 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2027 2059 2027 Size: 32 5612 PstI C_TGCA'G Cuts at: 830 2226 2996 830 Size: 1396 770 3478 Fragments arranged by size: 3478 1396 770 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5644 PvuII CAG'CTG Cuts at: 2137 2137 Size: 5644 SacI G_AGCT'C Cuts at: 2574 2574 Size: 5644 SalI G'TCGA_C Cuts at: 1936 2986 1936 Size: 1050 4594 SbfI CC_TGCA'GG Cuts at: 2996 2996 Size: 5644 ScaI AGT'ACT Cuts at: 3892 5604 3892 Size: 1712 3932 SexAI A'CCwGG_T Cuts at: 2403 2403 Size: 5644 SfcI C'TryA_G Cuts at: 826 1045 2222 2992 4127 4808 4999 826 Size: 219 1177 770 1135 681 191 1471 Fragments arranged by size: 1471 1177 1135 770 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5644 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5644 SmaI CCC'GGG Cuts at: 1872 2459 2696 2971 1872 Size: 587 237 275 4545 Fragments arranged by size: 4545 587 275 237 SmlI C'TyrA_G Cuts at: 820 1017 1943 3748 4649 4911 820 Size: 197 926 1805 901 262 1553 Fragments arranged by size: 1805 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3435 493 Size: 2942 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5644 SphI G_CATG'C Cuts at: 1132 2155 2662 2804 3002 1132 Size: 1023 507 142 198 3774 Fragments arranged by size: 3774 1023 507 198 142 SspI AAT'ATT Cuts at: 5 52 3568 5 Size: 47 3516 2081 Fragments arranged by size: 3516 2081 47 StuI AGG'CCT Cuts at: 1517 2307 2546 1517 Size: 790 239 4615 Fragments arranged by size: 4615 790 239 StyI C'CwwG_G Cuts at: 513 1065 2004 2370 2442 2924 3083 513 Size: 552 939 366 72 482 159 3074 Fragments arranged by size: 3074 939 552 482 366 159 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2269 3671 3688 3841 4026 4471 325 Size: 1255 689 1402 17 153 185 445 1498 Fragments arranged by size: 1498 1402 1255 689 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3890 5602 96 Size: 274 80 33 51 1443 1913 1712 138 Fragments arranged by size: 1913 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5604 5636 5604 Size: 32 5612 Tth111I GACn'n_nGTC Cuts at: 1316 2939 1316 Size: 1623 4021 XbaI T'CTAG_A Cuts at: 2980 2980 Size: 5644 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5644 XmaI C'CCGG_G Cuts at: 1870 2457 2694 2969 1870 Size: 587 237 275 4545 Fragments arranged by size: 4545 587 275 237 XmnI GAAnn'nnTTC Cuts at: 3773 3773 Size: 5644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3452 276 Size: 53 83 186 936 1918 2468 Fragments arranged by size: 2468 1918 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI