################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BbrPI BcgI BclI BfrI BglI BlnI BmyI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcyI 733 GGCGTC G[GA]^CG[CT]C AlwI 631 GATCC GGATC(4/5) AvaI 25 CCCGGG C^[CT]CG[GA]G BanI 93 GGCACC G^G[CT][GA]CC BbsI 861 GTCTTC GAAGAC(2/6) BbvCI 1044 CCTCAGC CCTCAGC(-5/-2) BciVI 494 GTATCC GTATCC(6/5) BfaI 101 CTAG C^TAG BglII 210 AGATCT A^GATCT BpuAI 861 GTCTTC GAAGAC(2/6) BsaAI 1059 CACGTA [CT]AC^GT[GA] BsaHI 733 GGCGTC G[GA]^CG[CT]C BsgI 830 CTGCAC GTGCAG(16/14) BsmI 720 GAATGC GAATGC(1/-1) BsoBI 25 CCCGGG C^[CT]CG[GA]G Bsp1407I 363 TGTACA T^GTACA BsrDI 140 GCAATG GCAATG(2/0) BsrGI 363 TGTACA T^GTACA BstXI 579 CCACTTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 210 AGATCT [GA]^GATC[CT] DrdI 727 GACATGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 767 CTCTTC CTCTTC(1/4) FauI 659 GCGGG CCCGC(4/6) HapII 26 CCGG C^CGG Hin1I 733 GGCGTC G[GA]^CG[CT]C HpaII 26 CCGG C^CGG Ksp632I 767 CTCTTC CTCTTC(1/4) MaeI 101 CTAG C^TAG MaeII 1060 ACGT A^CGT MflI 210 AGATCT [GA]^GATC[CT] MspI 26 CCGG C^CGG PflMI 1023 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1076 CTGCAG CTGCA^G PvuII 154 CAGCTG CAG^CTG SapI 766 GCTCTTC GCTCTTC(1/4) SfcI 1076 CTGCAG C^T[GA][CT]AG SmaI 25 CCCGGG CCC^GGG SspBI 363 TGTACA T^GTACA Van91I 1023 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 210 AGATCT [GA]^GATC[CT] XmaCI 25 CCCGGG C^CCGGG XmaI 25 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwNI 445 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 1024 CAGCAACTG BalI 474 TGGCCA TGG^CCA 712 TGGCCA BcnI 25 CCCGG CC^[GC]GG 26 CCGGG BlpI 82 GCTCAGC GC^T[ACGT]AGC 156 GCTGAGC Bpu1102I 82 GCTCAGC GC^T[ACGT]AGC 156 GCTGAGC BseRI 890 CTCCTC GAGGAG(10/8) 1141 GAGGAG BsmFI 514 GGGAC GGGAC(10/14) 1011 GGGAC CelII 82 GCTCAGC GC^T[ACGT]AGC 156 GCTGAGC DpnII 211 GATC ^GATC 631 GATC DraII 395 AGGACCC [GA]G^G[ACGT]CC[CT] 1011 GGGACCC EcoO109I 395 AGGACCC [GA]G^G[ACGT]CC[CT] 1011 GGGACCC EcoT14I 681 CCAAGG C^C[AT][AT]GG 715 CCAAGG HgaI 1177 GACGC GACGC(5/10) 734 GCGTC HinfI 547 GAGTC G^A[ACGT]TC 879 GACTC HphI 575 TCACC GGTGA(8/7) 803 TCACC MboI 211 GATC ^GATC 631 GATC MluNI 474 TGGCCA TGG^CCA 712 TGGCCA MscI 474 TGGCCA TGG^CCA 712 TGGCCA MseI 584 TTAA T^TAA 599 TTAA NciI 25 CCCGG CC^[GC]GG 26 CCGGG NdeI 68 CATATG CA^TATG 233 CATATG NdeII 211 GATC ^GATC 631 GATC PleI 879 GACTC GAGTC(4/5) 547 GAGTC PpuMI 395 AGGACCC [GA]G^G[AT]CC[CT] 1011 GGGACCC Sau3AI 211 GATC ^GATC 631 GATC StyI 681 CCAAGG C^C[AT][AT]GG 715 CCAAGG Tru9I 584 TTAA T^TAA 599 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BbvI BpmI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EaeI EcoRII Fnu4HI FokI HaeIII ItaI MaeIII MboII MnlI MslI MspA1I MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I