(Circular) (Six-base) MAPSORT of: pFN21AE0704.seq Check: 7382 from: 1 to: 5926 pFN21AE0704 5926 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2310 2375 2489 2759 2937 3989 5373 5641 Size: 678 65 114 270 178 1052 1384 268 Cuts at: 5641 1632 Size: 1917 Fragments arranged by size: 1917 1384 1052 678 270 268 178 114 65 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 4825 5802 1203 Size: 3622 977 1327 Fragments arranged by size: 3622 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 3119 1166 Size: 1953 3973 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3086 1184 Size: 1902 4024 AlwNI CAG_nnn'CTG Cuts at: 2455 3034 3669 5241 2455 Size: 579 635 1572 3140 Fragments arranged by size: 3140 1572 635 579 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 2410 2651 2693 3235 3461 3613 5722 1493 Size: 917 241 42 542 226 152 2109 1697 Fragments arranged by size: 2109 1697 917 542 241 226 152 42 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2030 3251 1870 Size: 73 87 1221 4545 Fragments arranged by size: 4545 1221 87 73 BamHI G'GATC_C Cuts at: 3256 3623 3256 Size: 367 5559 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2098 3247 4702 618 Size: 325 217 627 311 1149 1455 1842 Fragments arranged by size: 1842 1455 1149 627 325 311 217 BanII G_rGCy'C Cuts at: 1391 3245 1391 Size: 1854 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2859 928 Size: 971 960 3995 Fragments arranged by size: 3995 971 960 BbvCI CC'TCA_GC Cuts at: 3050 3050 Size: 5926 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4117 4151 1903 Size: 34 50 34 2096 34 3678 Fragments arranged by size: 3678 2096 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2510 3818 5028 2510 Size: 1308 1210 3408 Fragments arranged by size: 3408 1308 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4537 136 Size: 107 122 71 631 605 2865 1525 Fragments arranged by size: 2865 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2215 5921 2215 Size: 3706 2220 BlpI GC'TnA_GC Cuts at: 2088 2162 3343 2088 Size: 74 1181 4671 Fragments arranged by size: 4671 1181 74 Bme1580I G_kGCm'C Cuts at: 1790 3987 5143 1790 Size: 2197 1156 2573 Fragments arranged by size: 2573 2197 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3044 3103 3657 Size: 416 53 83 399 24 1627 59 554 Cuts at: 3657 4615 442 Size: 958 1753 Fragments arranged by size: 1753 1627 958 554 416 399 83 59 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2901 2901 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2477 2637 2981 4586 1239 Size: 1238 160 344 1605 2579 Fragments arranged by size: 2579 1605 1238 344 160 Bpu10I CC'TnA_GC Cuts at: 3050 3050 Size: 5926 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4051 4916 5214 4051 Size: 865 298 4763 Fragments arranged by size: 4763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4589 882 Size: 617 3090 2219 Fragments arranged by size: 3090 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3066 3717 493 Size: 711 1862 651 2702 Fragments arranged by size: 2702 1862 711 651 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2739 3733 4115 4750 Size: 53 83 186 936 1206 994 382 635 Cuts at: 4750 275 Size: 1451 Fragments arranged by size: 1451 1206 994 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4359 4757 5031 5178 5589 4359 Size: 398 274 147 411 4696 Fragments arranged by size: 4696 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5701 Fragments arranged by size: 5701 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2886 3161 1186 Size: 1700 275 3951 Fragments arranged by size: 3951 1700 275 BseYI C'CCAG_C Cuts at: 2791 3027 5129 5625 2791 Size: 236 2102 496 3092 Fragments arranged by size: 3092 2102 496 236 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2820 1163 Size: 573 1084 4269 Fragments arranged by size: 4269 1084 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3376 4137 5165 1540 Size: 275 186 1375 761 1028 2301 Fragments arranged by size: 2301 1375 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3987 4072 5143 3987 Size: 85 1071 4770 Fragments arranged by size: 4770 1071 85 BsmI GAATG_Cn' Cuts at: 2731 3437 3530 2731 Size: 706 93 5127 Fragments arranged by size: 5127 706 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3245 3987 4072 5143 1391 Size: 399 1455 742 85 1071 2174 Fragments arranged by size: 2174 1455 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3281 844 Size: 2437 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3813 1394 Size: 429 1990 3507 Fragments arranged by size: 3507 1990 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2152 4421 4595 65 Size: 1183 904 2269 174 1396 Fragments arranged by size: 2269 1396 1183 904 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4570 4757 1700 Size: 110 2760 187 2869 Fragments arranged by size: 2869 2760 187 110 BsrGI T'GTAC_A Cuts at: 96 2368 96 Size: 2272 3654 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 3986 4998 3986 Size: 1012 4914 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5926 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstXI CCAn_nnnn'nTGG Cuts at: 2591 2591 Size: 5926 BstYI r'GATC_y Cuts at: 1972 2215 3256 3623 4008 4025 5477 5563 5575 Size: 243 1041 367 385 17 1452 86 12 Cuts at: 5575 5921 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1041 385 367 346 243 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5374 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5187 Fragments arranged by size: 5187 683 56 BtsI GCAGTG_nn' Cuts at: 3183 3442 4236 4256 3183 Size: 259 794 20 4853 Fragments arranged by size: 4853 794 259 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2738 4933 809 Size: 507 1422 2195 1802 Fragments arranged by size: 2195 1802 1422 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2479 2717 3157 Size: 54 1425 50 275 18 649 238 440 Cuts at: 3157 3373 4262 5891 8 Size: 216 889 1629 43 Fragments arranged by size: 1629 1425 889 649 440 275 238 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2778 3856 1353 Size: 1425 1078 3423 Fragments arranged by size: 3423 1425 1078 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 2375 2477 2489 2637 2759 2937 Size: 393 678 65 102 12 148 122 178 Cuts at: 2937 2981 3989 4586 5373 5641 1239 Size: 44 1008 597 787 268 1524 Fragments arranged by size: 1524 1008 787 678 597 393 268 178 148 122 102 65 44 12 EcoO109I rG'GnC_Cy Cuts at: 2401 3017 2401 Size: 616 5310 EcoRI G'AATT_C Cuts at: 3235 3613 3235 Size: 378 5548 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 5073 1678 Size: 3395 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2812 2844 2881 2913 2968 Size: 32 135 32 1240 32 37 32 55 Cuts at: 2968 3000 3087 3119 3173 3205 4567 4599 4641 Size: 32 87 32 54 32 1362 32 42 Cuts at: 4641 4673 1373 Size: 32 2626 Fragments arranged by size: 2626 1362 1240 135 87 55 54 42 37 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5040 5224 1305 Size: 3735 184 2007 Fragments arranged by size: 3735 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2481 2719 10 Size: 54 1425 343 649 238 3217 Fragments arranged by size: 3217 1425 649 343 238 54 MslI CAynn'nnrTG Cuts at: 518 1166 2589 3068 3119 3884 4243 4402 518 Size: 648 1423 479 51 765 359 159 2042 Fragments arranged by size: 2042 1423 765 648 479 359 159 51 MspA1I CmG'CkG Cuts at: 1606 2161 2364 3156 3342 4019 5167 5412 1606 Size: 555 203 792 186 677 1148 245 2120 Fragments arranged by size: 2120 1148 792 677 555 245 203 186 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5374 NdeI CA'TA_TG Cuts at: 387 2074 2239 387 Size: 1687 165 4074 Fragments arranged by size: 4074 1687 165 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 3284 4829 1132 Size: 97 2055 1545 2229 Fragments arranged by size: 2229 2055 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5926 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 3034 1108 Size: 1926 4000 PfoI T'CCnGG_A Cuts at: 2918 2918 Size: 5926 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2401 3017 2401 Size: 616 5310 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PstI C_TGCA'G Cuts at: 830 3085 3278 830 Size: 2255 193 3478 Fragments arranged by size: 3478 2255 193 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 PvuII CAG'CTG Cuts at: 2161 2161 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SanDI GG'GwC_CC Cuts at: 3017 3017 Size: 5926 SapI GCTCTTCn'nnn_ Cuts at: 2778 2778 Size: 5926 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SfcI C'TryA_G Cuts at: 826 1045 3081 3274 4409 5090 5281 826 Size: 219 2036 193 1135 681 191 1471 Fragments arranged by size: 2036 1471 1135 681 219 193 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 2032 3253 1872 Size: 160 1221 4545 Fragments arranged by size: 4545 1221 160 SmlI C'TyrA_G Cuts at: 820 1017 1943 4030 4931 5193 820 Size: 197 926 2087 901 262 1553 Fragments arranged by size: 2087 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 3284 1132 Size: 2152 3774 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5926 StyI C'CwwG_G Cuts at: 513 1065 2686 2720 3365 513 Size: 552 1621 34 645 3074 Fragments arranged by size: 3074 1621 645 552 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2597 3953 3970 4123 4308 4753 325 Size: 1255 1017 1356 17 153 185 445 1498 Fragments arranged by size: 1498 1356 1255 1017 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2120 2368 3111 Size: 274 80 33 51 1443 143 248 743 Cuts at: 3111 4172 5884 96 Size: 1061 1712 138 Fragments arranged by size: 1712 1443 1061 743 274 248 143 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2682 2714 5886 5918 2682 Size: 32 3172 32 2690 Fragments arranged by size: 3172 2690 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 2030 3251 1870 Size: 160 1221 4545 Fragments arranged by size: 4545 1221 160 XmnI GAAnn'nnTTC Cuts at: 4055 4055 Size: 5926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI