(Linear) (Six-base) MAPSORT of: KIEE0704.seq Check: 5476 from: 1 to: 1221 KIEE0704.orf With 182 enzymes: SgfI * January 29, 2014 11:44 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 305 370 484 754 932 1221 Size: 305 65 114 270 178 289 Fragments arranged by size: 305 289 270 178 114 65 AleI CACnn'nnGTG Cuts at: 0 1114 1221 Size: 1114 107 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1081 1221 Size: 1081 140 AlwNI CAG_nnn'CTG Cuts at: 0 450 1029 1221 Size: 450 579 192 Fragments arranged by size: 579 450 192 ApoI r'AATT_y Cuts at: 0 405 646 688 1221 Size: 405 241 42 533 Fragments arranged by size: 533 405 241 42 AvaI C'yCGr_G Cuts at: 0 25 1221 Size: 25 1196 BanI G'GyrC_C Cuts at: 0 93 1221 Size: 93 1128 BbsI GAAGACnn'nnnn_ Cuts at: 0 854 1221 Size: 854 367 BbvCI CC'TCA_GC Cuts at: 0 1045 1221 Size: 1045 176 BciVI GTATCCnnnnn_n' Cuts at: 0 505 1221 Size: 505 716 BglII A'GATC_T Cuts at: 0 210 1221 Size: 210 1011 BlpI GC'TnA_GC Cuts at: 0 83 157 1221 Size: 83 74 1064 Fragments arranged by size: 1064 83 74 BmrI ACTGGGnnnn_n' Cuts at: 0 1039 1098 1221 Size: 1039 59 123 Fragments arranged by size: 1039 123 59 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 896 1221 Size: 896 325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 632 976 1221 Size: 472 160 344 245 Fragments arranged by size: 472 344 245 160 Bpu10I CC'TnA_GC Cuts at: 0 1045 1221 Size: 1045 176 BsaAI yAC'GTr Cuts at: 0 1061 1221 Size: 1061 160 BsaHI Gr'CG_yC Cuts at: 0 734 1221 Size: 734 487 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 881 1156 1221 Size: 881 275 65 Fragments arranged by size: 881 275 65 BseYI C'CCAG_C Cuts at: 0 786 1022 1221 Size: 786 236 199 Fragments arranged by size: 786 236 199 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 815 1221 Size: 815 406 BsmI GAATG_Cn' Cuts at: 0 726 1221 Size: 726 495 BsrDI GCAATG_nn' Cuts at: 0 147 1221 Size: 147 1074 BsrGI T'GTAC_A Cuts at: 0 363 1221 Size: 363 858 BstXI CCAn_nnnn'nTGG Cuts at: 0 586 1221 Size: 586 635 BstYI r'GATC_y Cuts at: 0 210 1221 Size: 210 1011 BtsI GCAGTG_nn' Cuts at: 0 1178 1221 Size: 1178 43 DrdI GACnn_nn'nnGTC Cuts at: 0 733 1221 Size: 733 488 EaeI y'GGCC_r Cuts at: 0 474 712 1152 1221 Size: 474 238 440 69 Fragments arranged by size: 474 440 238 69 EarI CTCTTCn'nnn_ Cuts at: 0 773 1221 Size: 773 448 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 305 370 472 484 632 754 932 976 Size: 305 65 102 12 148 122 178 44 Cuts at: 976 1221 Size: 245 Fragments arranged by size: 305 245 178 148 122 102 65 44 12 EcoO109I rG'GnC_Cy Cuts at: 0 396 1012 1221 Size: 396 616 209 Fragments arranged by size: 616 396 209 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 807 839 876 908 963 995 1082 1114 Size: 807 32 37 32 55 32 87 32 Cuts at: 1114 1168 1200 1221 Size: 54 32 21 Fragments arranged by size: 807 87 55 54 37 32 32 32 32 32 21 MscI TGG'CCA Cuts at: 0 476 714 1221 Size: 476 238 507 Fragments arranged by size: 507 476 238 MslI CAynn'nnrTG Cuts at: 0 584 1063 1114 1221 Size: 584 479 51 107 Fragments arranged by size: 584 479 107 51 MspA1I CmG'CkG Cuts at: 0 156 359 1151 1221 Size: 156 203 792 70 Fragments arranged by size: 792 203 156 70 NdeI CA'TA_TG Cuts at: 0 69 234 1221 Size: 69 165 987 Fragments arranged by size: 987 165 69 PflMI CCAn_nnn'nTGG Cuts at: 0 1029 1221 Size: 1029 192 PfoI T'CCnGG_A Cuts at: 0 913 1221 Size: 913 308 PpuMI rG'GwC_Cy Cuts at: 0 396 1012 1221 Size: 396 616 209 Fragments arranged by size: 616 396 209 PstI C_TGCA'G Cuts at: 0 1080 1221 Size: 1080 141 PvuII CAG'CTG Cuts at: 0 156 1221 Size: 156 1065 SanDI GG'GwC_CC Cuts at: 0 1012 1221 Size: 1012 209 SapI GCTCTTCn'nnn_ Cuts at: 0 773 1221 Size: 773 448 SfcI C'TryA_G Cuts at: 0 1076 1221 Size: 1076 145 SmaI CCC'GGG Cuts at: 0 27 1221 Size: 27 1194 StyI C'CwwG_G Cuts at: 0 681 715 1221 Size: 681 34 506 Fragments arranged by size: 681 506 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 592 1221 Size: 592 629 TatI w'GTAC_w Cuts at: 0 115 363 1106 1221 Size: 115 248 743 115 Fragments arranged by size: 743 248 115 115 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 677 709 1221 Size: 677 32 512 Fragments arranged by size: 677 512 32 XmaI C'CCGG_G Cuts at: 0 25 1221 Size: 25 1196 Enzymes that do cut: AcuI AleI AlfI AlwNI ApoI AvaI BanI BbsI BbvCI BciVI BglII BlpI BmrI BplI BpmI Bpu10I BsaAI BsaHI BseRI BseYI BsgI BsmI BsrDI BsrGI BstXI BstYI BtsI DrdI EaeI EarI Eco57MI EcoO109I Hin4I MscI MslI MspA1I NdeI PflMI PfoI PpuMI PstI PvuII SanDI SapI SfcI SmaI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BcgI BclI BfrBI BglI Bme1580I BmgBI BmtI BpuEI BsaI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI