Restriction map for
pFN21AE2289
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII AccII AccIII AclI AcsI
AflII AflIII AgeI Aor51HI ApoI
AscI AspI AvrII BalI BbrPI
BbvCI BcgI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FbaI FseI HincII
HindII HindIII HpaI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 107 GGTACC G^GTACC
AhdI 88 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 562 GTGCAC G^TGCAC
AlwNI 10 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1463 CCTCAGG CC^T[ACGT]AGG
ApaLI 562 GTGCAC G^TGCAC
AseI 1081 ATTAAT AT^TAAT
AsnI 1081 ATTAAT AT^TAAT
Asp700I 1388 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 107 GGTACC G^GTACC
AspEI 88 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 562 GTGCAC G[AT]GC[AT]^C
AvaI 374 CCCGGG C^[CT]CG[GA]G
AviII 617 TGCGCA TGC^GCA
BciVI 1485 GGATAC GTATCC(6/5)
BsiHKAI 562 GTGCAC G[AT]GC[AT]^C
BsoBI 374 CCCGGG C^[CT]CG[GA]G
BsrFI 710 ACCGGC [GA]^CCGG[CT]
BstXI 884 CCATGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1463 CCTCAGG CC^T[ACGT]AGG
Cfr10I 710 ACCGGC [GA]^CCGG[CT]
Eam1105I 88 GACTACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1463 CCTCAGG CC^T[ACGT]AGG
EcoRV 154 GATATC GAT^ATC
FauI 233 CCCGC CCCGC(4/6)
FspI 617 TGCGCA TGC^GCA
HgaI 387 GCGTC GACGC(5/10)
KpnI 107 GGTACC GGTAC^C
MaeII 1513 ACGT A^CGT
MaeIII 96 GTCAC ^GT[ACGT]AC
MslI 730 CACCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 474 CCATGG C^CATGG
NheI 1215 GCTAGC G^CTAGC
PshBI 1081 ATTAAT AT^TAAT
SfiI 681 GGCCCTCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 374 CCCGGG CCC^GGG
SspI 1390 AATATT AAT^ATT
Tsp45I 96 GTCAC ^GT[GC]AC
Tsp509I 1084 AATT ^AATT
XbaI 123 TCTAGA T^CTAGA
XcmI 753 CCAGGCTCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 374 CCCGGG C^CCGGG
XmaI 374 CCCGGG C^CCGGG
XmnI 1388 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 78 GGCGCC G[GA]^CG[CT]C
1346 GGCGCC
BamHI 264 GGATCC G^GATCC
749 GGATCC
BbeI 78 GGCGCC GGCGC^C
1346 GGCGCC
BglI 223 GCCACAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
682 GCCCTCATGGC
BpmI 309 CTGGAG CTGGAG(16/14)
405 CTGGAG
BsaHI 78 GGCGCC G[GA]^CG[CT]C
1346 GGCGCC
BsgI 727 CTGCAC GTGCAG(16/14)
439 GTGCAG
BspMI 344 ACCTGC ACCTGC(4/8)
614 ACCTGC
BsrDI 624 CATTGC GCAATG(2/0)
410 GCAATG
EaeI 477 TGGCCG [CT]^GGCC[GA]
1311 TGGCCG
EarI 771 GAAGAG CTCTTC(1/4)
802 GAAGAG
EcoT14I 474 CCATGG C^C[AT][AT]GG
1481 CCTTGG
Hin1I 78 GGCGCC G[GA]^CG[CT]C
1346 GGCGCC
HphI 609 GGTGA GGTGA(8/7)
158 TCACC
KasI 78 GGCGCC G^GCGCC
1346 GGCGCC
Ksp632I 771 GAAGAG CTCTTC(1/4)
802 GAAGAG
NarI 78 GGCGCC GG^CGCC
1346 GGCGCC
PpuMI 56 GGGTCCT [GA]G^G[AT]CC[CT]
595 GGGACCT
PvuII 13 CAGCTG CAG^CTG
211 CAGCTG
SfcI 112 CTACAG C^T[GA][CT]AG
1427 CTATAG
SfoI 78 GGCGCC GGC^GCC
1346 GGCGCC
StyI 474 CCATGG C^C[AT][AT]GG
1481 CCTTGG
TfiI 949 GATTC G^A[AT]TC
1044 GATTC
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
ApaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EcoNI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeI MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI Tru9I TseI
TspRI TthHB8I Van91I XhoII