(Circular) (Six-base) MAPSORT of: pFN21AE2289.seq Check: 9570 from: 1 to: 6229 pFN21AE2289 6229 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4039 278 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2703 3027 3324 3345 3572 1937 Size: 766 324 297 21 227 4594 Fragments arranged by size: 4594 766 324 297 227 21 Acc65I G'GTAC_C Cuts at: 2112 3550 2112 Size: 1438 4791 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3220 4292 5676 5944 1632 Size: 1588 1072 1384 268 1917 Fragments arranged by size: 1917 1588 1384 1072 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 5128 6105 1203 Size: 3925 977 1327 Fragments arranged by size: 3925 1327 977 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 2098 4958 2098 Size: 2860 3369 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2469 1184 Size: 1285 4944 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2339 2371 2339 Size: 32 6197 AlwNI CAG_nnn'CTG Cuts at: 2020 3972 5544 2020 Size: 1952 1572 2705 Fragments arranged by size: 2705 1952 1572 ApaI G_GGCC'C Cuts at: 2239 2904 2969 2239 Size: 665 65 5499 Fragments arranged by size: 5499 665 65 ApaLI G'TGCA_C Cuts at: 2567 4286 5442 2567 Size: 1719 1156 3354 Fragments arranged by size: 3354 1719 1156 ApoI r'AATT_y Cuts at: 1493 3538 3764 3916 6025 1493 Size: 2045 226 152 2109 1697 Fragments arranged by size: 2109 2045 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3087 4783 160 Size: 872 2055 1696 1606 Fragments arranged by size: 2055 1696 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 2379 3554 1870 Size: 73 436 1175 4545 Fragments arranged by size: 4545 1175 436 73 BamHI G'GATC_C Cuts at: 2269 2754 3559 3926 2269 Size: 485 805 367 4572 Fragments arranged by size: 4572 805 485 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2083 2112 3351 3550 5005 Size: 325 217 627 296 29 1239 199 1455 Cuts at: 5005 618 Size: 1842 Fragments arranged by size: 1842 1455 1239 627 325 296 217 199 29 BanII G_rGCy'C Cuts at: 1391 2239 2904 2969 3003 3197 3548 1391 Size: 848 665 65 34 194 351 4072 Fragments arranged by size: 4072 848 665 351 194 65 34 BbeI G_GCGC'C Cuts at: 2087 3355 2087 Size: 1268 4961 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2291 2586 2918 928 Size: 971 392 295 332 4239 Fragments arranged by size: 4239 971 392 332 295 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4420 4454 1903 Size: 34 2483 34 3678 Fragments arranged by size: 3678 2483 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3484 4121 5331 3484 Size: 637 1210 4382 Fragments arranged by size: 4382 1210 637 BclI T'GATC_A Cuts at: 1548 1548 Size: 6229 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2234 2693 4840 Size: 107 122 71 631 605 562 459 2147 Cuts at: 4840 136 Size: 1525 Fragments arranged by size: 2147 1525 631 605 562 459 122 107 71 BglII A'GATC_T Cuts at: 6224 6224 Size: 6229 BlpI GC'TnA_GC Cuts at: 3646 3646 Size: 6229 Bme1580I G_kGCm'C Cuts at: 1790 2239 2571 2670 2832 2841 2904 2969 4290 Size: 449 332 99 162 9 63 65 1321 Cuts at: 4290 5446 1790 Size: 1156 2573 Fragments arranged by size: 2573 1321 1156 449 332 162 99 65 63 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6229 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2693 2828 2844 Size: 416 53 83 399 24 1276 135 16 Cuts at: 2844 3960 4918 442 Size: 1116 958 1753 Fragments arranged by size: 1753 1276 1116 958 416 399 135 83 53 24 16 BmtI G_CTAG'C Cuts at: 1056 3224 1056 Size: 2168 4061 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2268 2268 Size: 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2335 2431 4889 1239 Size: 1096 96 2458 2579 Fragments arranged by size: 2579 2458 1096 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4354 5219 5517 4354 Size: 865 298 5066 Fragments arranged by size: 5066 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4892 882 Size: 617 3393 2219 Fragments arranged by size: 3393 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4020 493 Size: 711 2816 2702 Fragments arranged by size: 2816 2702 711 BsaBI GATnn'nnATC Cuts at: 6223 6223 Size: 6229 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2084 3352 4036 4418 Size: 53 83 186 936 551 1268 684 382 Cuts at: 4418 5053 275 Size: 635 1451 Fragments arranged by size: 1451 1268 936 684 635 551 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4662 5060 5334 5481 5892 4662 Size: 398 274 147 411 4999 Fragments arranged by size: 4999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2339 2369 3057 3087 1178 Size: 30 165 30 936 30 688 30 4320 Fragments arranged by size: 4320 936 688 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6229 BseYI C'CCAG_C Cuts at: 2026 2305 2557 2896 5432 5928 2026 Size: 279 252 339 2536 496 2327 Fragments arranged by size: 2536 2327 496 339 279 252 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2465 2717 1163 Size: 573 729 252 4675 Fragments arranged by size: 4675 729 573 252 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3679 4440 5468 1540 Size: 275 186 1678 761 1028 2301 Fragments arranged by size: 2301 1678 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2571 4290 4375 5446 2571 Size: 1719 85 1071 3354 Fragments arranged by size: 3354 1719 1071 85 BsmI GAATG_Cn' Cuts at: 3740 3833 3740 Size: 93 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2239 2571 2670 2832 2841 2904 2969 Size: 399 449 332 99 162 9 63 65 Cuts at: 2969 3003 3197 3548 4290 4375 5446 1391 Size: 34 194 351 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 449 399 351 332 194 162 99 85 65 63 34 9 BspEI T'CCGG_A Cuts at: 5892 5892 Size: 6229 BspHI T'CATG_A Cuts at: 4118 5848 4118 Size: 1730 4499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2358 2628 3584 844 Size: 1514 270 956 3489 Fragments arranged by size: 3489 1514 956 270 BsrBI CCG'CTC Cuts at: 1394 1823 4116 1394 Size: 429 2293 3507 Fragments arranged by size: 3507 2293 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2422 2628 4724 4898 65 Size: 1183 1174 206 2096 174 1396 Fragments arranged by size: 2096 1396 1183 1174 206 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2715 4873 5060 1700 Size: 110 905 2158 187 2869 Fragments arranged by size: 2869 2158 905 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6229 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6229 BssSI C'ACGA_G Cuts at: 4289 5301 4289 Size: 1012 5217 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6229 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6229 BstXI CCAn_nnnn'nTGG Cuts at: 2896 2896 Size: 6229 BstYI r'GATC_y Cuts at: 1972 2135 2269 2754 3559 3926 4311 4328 5780 Size: 163 134 485 805 367 385 17 1452 Cuts at: 5780 5866 5878 6224 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 805 485 385 367 346 163 134 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3469 3469 Size: 6229 BtgI C'CryG_G Cuts at: 513 1065 2088 2479 2608 2905 513 Size: 552 1023 391 129 297 3837 Fragments arranged by size: 3837 1023 552 391 297 129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5490 Fragments arranged by size: 5490 683 56 BtsI GCAGTG_nn' Cuts at: 2569 3215 3745 4539 4559 2569 Size: 646 530 794 20 4239 Fragments arranged by size: 4239 794 646 530 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 3533 3533 Size: 6229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2482 3316 3676 Size: 54 1425 50 275 18 652 834 360 Cuts at: 3676 4565 6194 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 834 652 360 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3676 1537 Size: 275 1864 4090 Fragments arranged by size: 4090 1864 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2771 2802 4159 1353 Size: 1418 31 1357 3423 Fragments arranged by size: 3423 1418 1357 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2456 4816 5190 5336 387 Size: 1064 138 867 2360 374 146 1280 Fragments arranged by size: 2360 1280 1064 867 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2335 2431 3220 4292 4889 5676 5944 Size: 393 703 96 789 1072 597 787 268 Cuts at: 5944 1239 Size: 1524 Fragments arranged by size: 1524 1072 789 787 703 597 393 268 96 EcoNI CCTnn'n_nnAGG Cuts at: 2130 2993 3188 2130 Size: 863 195 5171 Fragments arranged by size: 5171 863 195 EcoO109I rG'GnC_Cy Cuts at: 2062 2235 2601 2686 2900 2901 2966 2062 Size: 173 366 85 214 1 65 5325 Fragments arranged by size: 5325 366 214 173 85 65 1 EcoRI G'AATT_C Cuts at: 3538 3916 3538 Size: 378 5851 EcoRV GAT'ATC Cuts at: 2161 2161 Size: 6229 FspI TGC'GCA Cuts at: 2624 4735 2624 Size: 2111 4118 HaeII r_GCGC'y Cuts at: 1678 2087 3082 3355 5376 1678 Size: 409 995 273 2021 2531 Fragments arranged by size: 2531 2021 995 409 273 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 2746 2778 3403 Size: 32 135 32 512 32 630 32 625 Cuts at: 3403 3435 4870 4902 4944 4976 1373 Size: 32 1435 32 42 32 2626 Fragments arranged by size: 2626 1435 630 625 512 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3573 3819 669 Size: 988 281 1635 246 3079 Fragments arranged by size: 3079 1635 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KasI G'GCGC_C Cuts at: 2083 3351 2083 Size: 1268 4961 KpnI G_GTAC'C Cuts at: 2116 3554 2116 Size: 1438 4791 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5343 5527 1305 Size: 4038 184 2007 Fragments arranged by size: 4038 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4407 Fragments arranged by size: 4407 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2739 4187 4546 4705 518 Size: 648 1573 1448 359 159 2042 Fragments arranged by size: 2042 1573 1448 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2020 2218 2313 3645 4322 5470 5715 1606 Size: 414 198 95 1332 677 1148 245 2120 Fragments arranged by size: 2120 1332 1148 677 414 245 198 95 NarI GG'CG_CC Cuts at: 2084 3352 2084 Size: 1268 4961 NcoI C'CATG_G Cuts at: 513 1065 2479 513 Size: 552 1414 4263 Fragments arranged by size: 4263 1414 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6229 NheI G'CTAG_C Cuts at: 1052 3220 1052 Size: 2168 4061 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 3587 5132 1132 Size: 97 2358 1545 2229 Fragments arranged by size: 2358 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6229 PciI A'CATG_T Cuts at: 5128 5128 Size: 6229 PflMI CCAn_nnn'nTGG Cuts at: 1108 2644 2656 3452 1108 Size: 1536 12 796 3885 Fragments arranged by size: 3885 1536 796 12 PfoI T'CCnGG_A Cuts at: 2403 2403 Size: 6229 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2339 2371 4298 4330 5811 5843 2339 Size: 32 1927 32 1481 32 2725 Fragments arranged by size: 2725 1927 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2062 2601 2062 Size: 539 5690 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PspOMI G'GGCC_C Cuts at: 2235 2900 2965 2235 Size: 665 65 5499 Fragments arranged by size: 5499 665 65 PstI C_TGCA'G Cuts at: 830 3581 830 Size: 2751 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 PvuII CAG'CTG Cuts at: 2020 2218 2020 Size: 198 6031 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 4477 6189 4477 Size: 1712 4517 SfcI C'TryA_G Cuts at: 826 1045 2117 3432 3577 4712 5393 5584 826 Size: 219 1072 1315 145 1135 681 191 1471 Fragments arranged by size: 1471 1315 1135 1072 681 219 191 145 SfiI GGCCn_nnn'nGGCC Cuts at: 2693 2693 Size: 6229 SfoI GGC'GCC Cuts at: 2085 3353 2085 Size: 1268 4961 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 2381 3556 1872 Size: 509 1175 4545 Fragments arranged by size: 4545 1175 509 SmlI C'TyrA_G Cuts at: 820 1017 1943 4333 5234 5496 820 Size: 197 926 2390 901 262 1553 Fragments arranged by size: 2390 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3587 1132 Size: 2455 3774 SrfI GCCC'GGGC Cuts at: 2381 2381 Size: 6229 SspI AAT'ATT Cuts at: 5 52 3397 4153 5 Size: 47 3345 756 2081 Fragments arranged by size: 3345 2081 756 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6229 StyI C'CwwG_G Cuts at: 513 1065 2479 3486 3668 513 Size: 552 1414 1007 182 3074 Fragments arranged by size: 3074 1414 1007 552 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2751 4256 4273 4426 4611 5056 325 Size: 1255 1171 1505 17 153 185 445 1498 Fragments arranged by size: 1505 1498 1255 1171 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4475 6187 96 Size: 274 80 33 51 1443 2498 1712 138 Fragments arranged by size: 2498 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2593 2625 2924 2956 3057 3089 6189 6221 2593 Size: 32 299 32 101 32 3100 32 2601 Fragments arranged by size: 3100 2601 299 101 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6229 XbaI T'CTAG_A Cuts at: 2128 3565 2128 Size: 1437 4792 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2765 2765 Size: 6229 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6229 XmaI C'CCGG_G Cuts at: 1870 2379 3554 1870 Size: 509 1175 4545 Fragments arranged by size: 4545 1175 509 XmnI GAAnn'nnTTC Cuts at: 3397 4358 3397 Size: 961 5268 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4037 276 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SwaI