################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccIII AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eam1105I EarI Eco81I EcoO65I EcoRI EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AccI 577 GTCTAC GT^[AC][GT]AC AclI 881 AACGTT AA^CGTT AflIII 1040 ACATGT A^C[GA][CT]GT BciVI 659 GTATCC GTATCC(6/5) BsaI 418 GAGACC GGTCTC(1/5) BsiEI 71 CGGCCG CG[GA][CT]^CG BsmBI 442 GAGACG CGTCTC(1/5) BspLU11I 1040 ACATGT A^CATGT BspMI 581 ACCTGC ACCTGC(4/8) BsrBI 663 CCGCTC CCGCTC(-3/-3) DrdI 802 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 71 CGGCCG C^GGCCG EclXI 71 CGGCCG C^GGCCG Eco52I 71 CGGCCG C^GGCCG EcoNI 387 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 944 GATATC GAT^ATC KspI 83 CCGCGG CCGC^GG MaeII 882 ACGT A^CGT MboII 672 GAAGA GAAGA(8/7) Psp1406I 881 AACGTT AA^CGTT SacII 83 CCGCGG CCGC^GG SmaI 59 CCCGGG CCC^GGG XcmI 751 CCACAGAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 59 CCCGGG C^CCGGG XmaI 59 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AlwNI 304 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 334 CAGATCCTG Aor51HI 137 AGCGCT AGC^GCT 1001 AGCGCT ApaI 78 GGGCCC GGGCC^C 1105 GGGCCC AspHI 4 GTGCTC G[AT]GC[AT]^C 916 GAGCAC BanII 78 GGGCCC G[GA]GC[CT]^C 1105 GGGCCC BfaI 226 CTAG C^TAG 868 CTAG BglII 245 AGATCT A^GATCT 587 AGATCT BseRI 37 CTCCTC GAGGAG(10/8) 391 GAGGAG BsgI 682 CTGCAC GTGCAG(16/14) 1075 GTGCAG BsiHKAI 4 GTGCTC G[AT]GC[AT]^C 916 GAGCAC BsmAI 418 GAGAC GTCTC(1/5) 442 GAGAC BsrFI 146 GCCGGC [GA]^CCGG[CT] 644 GCCGGC BstAPI 303 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 585 GCAGATCTTGC BstF5I 654 CATCC GGATG(2/0) 844 CATCC Cfr10I 146 GCCGGC [GA]^CCGG[CT] 644 GCCGGC DdeI 693 CTCAG C^T[ACGT]AG 998 CTAAG DsaI 83 CCGCGG C^C[GA][CT]GG 161 CCGTGG Eco47III 137 AGCGCT AGC^GCT 1001 AGCGCT EcoT14I 314 CCAAGG C^C[AT][AT]GG 503 CCAAGG FokI 654 CATCC GGATG(9/13) 844 CATCC HgaI 364 GACGC GACGC(5/10) 648 GCGTC HinfI 863 GAGTC G^A[ACGT]TC 928 GAGTC MaeI 226 CTAG C^TAG 868 CTAG MaeIII 650 GTCAC ^GT[ACGT]AC 811 GTCAC NaeI 146 GCCGGC GCC^GGC 644 GCCGGC NgoMIV 146 GCCGGC G^CCGGC 644 GCCGGC NspI 1040 ACATGT [GA]CATG^[CT] 1142 ACATGC PleI 863 GAGTC GAGTC(4/5) 928 GAGTC StyI 314 CCAAGG C^C[AT][AT]GG 503 CCAAGG Tsp45I 650 GTCAC ^GT[GC]AC 811 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaI AvaII BanI BbeI BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI BstUI BstYI Cac8I CfoI Cfr13I DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI MboI MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI TaqI TseI TspRI TthHB8I XhoII