(Circular) (Six-base) MAPSORT of: pFN21AE0233.seq Check: 4736 from: 1 to: 5926 pFN21AE0233 5926 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2583 3269 1937 Size: 646 686 4594 Fragments arranged by size: 4594 686 646 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 2887 4053 4426 1563 Size: 1324 1166 373 3063 Fragments arranged by size: 3063 1324 1166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2485 2552 2696 3989 5373 5641 1632 Size: 853 67 144 1293 1384 268 1917 Fragments arranged by size: 1917 1384 1293 853 268 144 67 AfeI AGC'GCT Cuts at: 2144 3008 2144 Size: 864 5062 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 3045 4825 5802 1203 Size: 1842 1780 977 1327 Fragments arranged by size: 1842 1780 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AlwNI CAG_nnn'CTG Cuts at: 2314 2344 3669 5241 2314 Size: 30 1325 1572 2999 Fragments arranged by size: 2999 1572 1325 30 ApaI G_GGCC'C Cuts at: 2087 3114 2087 Size: 1027 4899 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 3235 3461 3613 5722 1493 Size: 1742 226 152 2109 1697 Fragments arranged by size: 2109 1742 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2064 2174 2381 3251 1870 Size: 73 121 110 207 870 4545 Fragments arranged by size: 4545 870 207 121 110 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2647 2680 2647 Size: 33 5893 BamHI G'GATC_C Cuts at: 3256 3623 3256 Size: 367 5559 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2170 2278 2323 3247 4702 Size: 325 217 627 383 108 45 924 1455 Cuts at: 4702 618 Size: 1842 Fragments arranged by size: 1842 1455 924 627 383 325 217 108 45 BanII G_rGCy'C Cuts at: 1391 2087 3114 3245 1391 Size: 696 1027 131 4072 Fragments arranged by size: 4072 1027 696 131 BbeI G_GCGC'C Cuts at: 2174 2282 2327 2174 Size: 108 45 5773 Fragments arranged by size: 5773 108 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4955 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4117 4151 1903 Size: 34 2180 34 3678 Fragments arranged by size: 3678 2180 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2675 3818 5028 2675 Size: 1143 1210 3573 Fragments arranged by size: 3573 1210 1143 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4537 136 Size: 107 122 71 631 605 2865 1525 Fragments arranged by size: 2865 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2250 2592 5921 2250 Size: 342 3329 2255 Fragments arranged by size: 3329 2255 342 BlpI GC'TnA_GC Cuts at: 3343 3343 Size: 5926 Bme1580I G_kGCm'C Cuts at: 1790 2087 3114 3987 5143 1790 Size: 297 1027 873 1156 2573 Fragments arranged by size: 2573 1156 1027 873 297 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2625 2776 2790 Size: 416 53 83 399 24 1208 151 14 Cuts at: 2790 3657 4615 442 Size: 867 958 1753 Fragments arranged by size: 1753 1208 958 867 416 399 151 83 53 24 14 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2707 2707 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 2131 2414 4586 1239 Size: 833 59 283 2172 2579 Fragments arranged by size: 2579 2172 833 283 59 Bpu10I CC'TnA_GC Cuts at: 2698 3003 2698 Size: 305 5621 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2255 2275 2911 4051 4916 5214 2255 Size: 20 636 1140 865 298 2967 Fragments arranged by size: 2967 1140 865 636 298 20 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2417 4589 882 Size: 617 918 2172 2219 Fragments arranged by size: 2219 2172 918 617 BsaAI yAC'GTr Cuts at: 493 1204 3717 493 Size: 711 2513 2702 Fragments arranged by size: 2702 2513 711 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2171 2279 2324 2653 Size: 53 83 186 936 638 108 45 329 Cuts at: 2653 3733 4115 4750 275 Size: 1080 382 635 1451 Fragments arranged by size: 1451 1080 936 638 635 382 329 186 108 83 53 45 BsaWI w'CCGG_w Cuts at: 4359 4757 5031 5178 5589 4359 Size: 398 274 147 411 4696 Fragments arranged by size: 4696 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2390 2420 3011 3041 1178 Size: 30 165 30 987 30 591 30 4063 Fragments arranged by size: 4063 987 591 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2411 1186 Size: 847 378 4701 Fragments arranged by size: 4701 847 378 BseYI C'CCAG_C Cuts at: 2029 2455 2538 2572 2982 3107 5129 5625 2029 Size: 426 83 34 410 125 2022 496 2330 Fragments arranged by size: 2330 2022 496 426 410 125 83 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2672 3101 1163 Size: 573 936 429 3988 Fragments arranged by size: 3988 936 573 429 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 3376 4137 5165 1540 Size: 275 186 78 1297 761 1028 2301 Fragments arranged by size: 2301 1297 1028 761 275 186 78 BsiHKAI G_wGCw'C Cuts at: 2013 2925 3987 4072 5143 2013 Size: 912 1062 85 1071 2796 Fragments arranged by size: 2796 1071 1062 912 85 BsmI GAATG_Cn' Cuts at: 3437 3530 3437 Size: 93 5833 BsmBI CGTCTCn'nnnn_ Cuts at: 2441 2441 Size: 5926 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2087 2925 3114 3245 3987 4072 Size: 399 223 74 838 189 131 742 85 Cuts at: 4072 5143 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 838 742 399 223 189 131 85 74 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2595 3281 844 Size: 1751 686 3489 Fragments arranged by size: 3489 1751 686 BsrBI CCG'CTC Cuts at: 1394 1823 2670 3813 1394 Size: 429 847 1143 3507 Fragments arranged by size: 3507 1143 847 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 4595 65 Size: 1183 3173 174 1396 Fragments arranged by size: 3173 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2151 2649 4570 4757 1700 Size: 110 341 498 1921 187 2869 Fragments arranged by size: 2869 1921 498 341 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 3986 4998 3986 Size: 1012 4914 BstAPI GCAn_nnn'nTGC Cuts at: 794 2314 2596 794 Size: 1520 282 4124 Fragments arranged by size: 4124 1520 282 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstYI r'GATC_y Cuts at: 1972 2250 2340 2592 3189 3256 3623 4008 4025 Size: 278 90 252 597 67 367 385 17 Cuts at: 4025 5477 5563 5575 5921 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 597 385 367 346 278 252 90 86 67 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2088 2166 513 Size: 552 939 84 78 4273 Fragments arranged by size: 4273 939 552 84 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2980 487 Size: 683 56 1754 3433 Fragments arranged by size: 3433 1754 683 56 BtsI GCAGTG_nn' Cuts at: 3442 4236 4256 3442 Size: 794 20 5112 Fragments arranged by size: 5112 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2813 4933 809 Size: 507 1497 2120 1802 Fragments arranged by size: 2120 1802 1497 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2153 3031 Size: 54 1425 50 275 18 246 77 878 Cuts at: 3031 3373 4262 5891 8 Size: 342 889 1629 43 Fragments arranged by size: 1629 1425 889 878 342 275 246 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2076 3373 1537 Size: 275 264 1297 4090 Fragments arranged by size: 4090 1297 275 264 EarI CTCTTCn'nnn_ Cuts at: 1353 3856 1353 Size: 2503 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2658 2738 2911 4513 4887 5033 Size: 1064 138 1069 80 173 1602 374 146 Cuts at: 5033 387 Size: 1280 Fragments arranged by size: 1602 1280 1069 1064 374 173 146 138 80 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2131 2414 2485 2552 2696 3989 Size: 393 440 59 283 71 67 144 1293 Cuts at: 3989 4586 5373 5641 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1293 787 597 440 393 283 268 144 71 67 59 EcoNI CCTnn'n_nnAGG Cuts at: 2396 2396 Size: 5926 EcoO109I rG'GnC_Cy Cuts at: 2083 2084 3111 2083 Size: 1 1027 4898 Fragments arranged by size: 4898 1027 1 EcoRI G'AATT_C Cuts at: 3235 3613 3235 Size: 378 5548 EcoRV GAT'ATC Cuts at: 2951 2951 Size: 5926 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 2146 2174 2282 2327 3010 5073 1678 Size: 468 28 108 45 683 2063 2531 Fragments arranged by size: 2531 2063 683 468 108 45 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2909 2940 2941 2972 4567 Size: 32 135 32 1337 31 1 31 1595 Cuts at: 4567 4599 4641 4673 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1595 1337 135 42 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5926 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KasI G'GCGC_C Cuts at: 2170 2278 2323 2170 Size: 108 45 5773 Fragments arranged by size: 5773 108 45 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5040 5224 1305 Size: 3735 184 2007 Fragments arranged by size: 3735 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4104 Fragments arranged by size: 4104 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2435 3050 3131 3203 3884 4243 4402 Size: 648 1269 615 81 72 681 359 159 Cuts at: 4402 518 Size: 2042 Fragments arranged by size: 2042 1269 681 648 615 359 159 81 72 MspA1I CmG'CkG Cuts at: 1606 2023 2090 2123 2311 2332 2542 2742 2940 Size: 417 67 33 188 21 210 200 198 Cuts at: 2940 3342 4019 5167 5412 1606 Size: 402 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 417 402 245 210 200 198 188 67 33 21 NaeI GCC'GGC Cuts at: 2153 2651 2153 Size: 498 5428 NarI GG'CG_CC Cuts at: 2171 2279 2324 2171 Size: 108 45 5773 Fragments arranged by size: 5773 108 45 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4435 Fragments arranged by size: 4435 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NgoMIV G'CCGG_C Cuts at: 2151 2649 2151 Size: 498 5428 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 3049 3151 3284 4829 1132 Size: 97 1820 102 133 1545 2229 Fragments arranged by size: 2229 1820 1545 133 102 97 PasI CC'CwG_GG Cuts at: 1348 2824 1348 Size: 1476 4450 PciI A'CATG_T Cuts at: 3045 4825 3045 Size: 1780 4146 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5926 PfoI T'CCnGG_A Cuts at: 2343 2391 2343 Size: 48 5878 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3032 3064 3995 4027 5508 5540 3032 Size: 32 931 32 1481 32 3418 Fragments arranged by size: 3418 1481 931 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PspOMI G'GGCC_C Cuts at: 2083 3110 2083 Size: 1027 4899 PstI C_TGCA'G Cuts at: 830 2337 2379 2592 3278 830 Size: 1507 42 213 686 3478 Fragments arranged by size: 3478 1507 686 213 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 PvuII CAG'CTG Cuts at: 2311 2542 2742 2311 Size: 231 200 5495 Fragments arranged by size: 5495 231 200 SacII CC_GC'GG Cuts at: 2091 2091 Size: 5926 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SfcI C'TryA_G Cuts at: 826 1045 2333 2375 2588 3274 4409 5090 5281 Size: 219 1288 42 213 686 1135 681 191 Cuts at: 5281 826 Size: 1471 Fragments arranged by size: 1471 1288 1135 686 681 219 213 191 42 SfoI GGC'GCC Cuts at: 2172 2280 2325 2172 Size: 108 45 5773 Fragments arranged by size: 5773 108 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 2066 3253 1872 Size: 194 1187 4545 Fragments arranged by size: 4545 1187 194 SmlI C'TyrA_G Cuts at: 820 1017 1943 2254 2270 2926 4030 4931 5193 Size: 197 926 311 16 656 1104 901 262 Cuts at: 5193 820 Size: 1553 Fragments arranged by size: 1553 1104 926 901 656 311 262 197 16 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 3284 1132 Size: 2152 3774 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5926 StyI C'CwwG_G Cuts at: 513 1065 2004 2319 2508 3365 513 Size: 552 939 315 189 857 3074 Fragments arranged by size: 3074 939 857 552 315 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2536 3953 3970 4123 4308 4753 325 Size: 1255 956 1417 17 153 185 445 1498 Fragments arranged by size: 1498 1417 1255 956 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2461 3042 4172 Size: 274 80 33 51 1443 484 581 1130 Cuts at: 4172 5884 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1130 581 484 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5886 5918 5886 Size: 32 5894 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2763 2763 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 2064 3251 1870 Size: 194 1187 4545 Fragments arranged by size: 4545 1187 194 XmnI GAAnn'nnTTC Cuts at: 4055 4055 Size: 5926 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI