(Linear) (Six-base) MAPSORT of: KIEE0233.seq Check: 5378 from: 1 to: 1221 KIEE0233.orf With 182 enzymes: SgfI * January 29, 2014 11:44 .. AccI GT'mk_AC Cuts at: 0 578 1221 Size: 578 643 AclI AA'CG_TT Cuts at: 0 882 1221 Size: 882 339 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 480 547 691 1221 Size: 480 67 144 530 Fragments arranged by size: 530 480 144 67 AfeI AGC'GCT Cuts at: 0 139 1003 1221 Size: 139 864 218 Fragments arranged by size: 864 218 139 AflIII A'CryG_T Cuts at: 0 1040 1221 Size: 1040 181 AlwNI CAG_nnn'CTG Cuts at: 0 309 339 1221 Size: 309 30 882 Fragments arranged by size: 882 309 30 ApaI G_GGCC'C Cuts at: 0 82 1109 1221 Size: 82 1027 112 Fragments arranged by size: 1027 112 82 AvaI C'yCGr_G Cuts at: 0 59 169 376 1221 Size: 59 110 207 845 Fragments arranged by size: 845 207 110 59 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 642 675 1221 Size: 642 33 546 Fragments arranged by size: 642 546 33 BanI G'GyrC_C Cuts at: 0 165 273 318 1221 Size: 165 108 45 903 Fragments arranged by size: 903 165 108 45 BanII G_rGCy'C Cuts at: 0 82 1109 1221 Size: 82 1027 112 Fragments arranged by size: 1027 112 82 BbeI G_GCGC'C Cuts at: 0 169 277 322 1221 Size: 169 108 45 899 Fragments arranged by size: 899 169 108 45 BciVI GTATCCnnnnn_n' Cuts at: 0 670 1221 Size: 670 551 BglII A'GATC_T Cuts at: 0 245 587 1221 Size: 245 342 634 Fragments arranged by size: 634 342 245 Bme1580I G_kGCm'C Cuts at: 0 82 1109 1221 Size: 82 1027 112 Fragments arranged by size: 1027 112 82 BmrI ACTGGGnnnn_n' Cuts at: 0 620 771 785 1221 Size: 620 151 14 436 Fragments arranged by size: 620 436 151 14 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 702 1221 Size: 702 519 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 126 409 1221 Size: 67 59 283 812 Fragments arranged by size: 812 283 67 59 Bpu10I CC'TnA_GC Cuts at: 0 693 998 1221 Size: 693 305 223 Fragments arranged by size: 693 305 223 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 270 906 1221 Size: 250 20 636 315 Fragments arranged by size: 636 315 250 20 BsaI GGTCTCn'nnnn_ Cuts at: 0 412 1221 Size: 412 809 BsaHI Gr'CG_yC Cuts at: 0 166 274 319 648 1221 Size: 166 108 45 329 573 Fragments arranged by size: 573 329 166 108 45 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 385 415 1006 1036 1221 Size: 385 30 591 30 185 Fragments arranged by size: 591 385 185 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 28 406 1221 Size: 28 378 815 Fragments arranged by size: 815 378 28 BseYI C'CCAG_C Cuts at: 0 24 450 533 567 977 1102 1221 Size: 24 426 83 34 410 125 119 Fragments arranged by size: 426 410 125 119 83 34 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 667 1096 1221 Size: 667 429 125 Fragments arranged by size: 667 429 125 BsiEI CG_ry'CG Cuts at: 0 74 1221 Size: 74 1147 BsiHKAI G_wGCw'C Cuts at: 0 8 920 1221 Size: 8 912 301 Fragments arranged by size: 912 301 8 BsmBI CGTCTCn'nnnn_ Cuts at: 0 436 1221 Size: 436 785 Bsp1286I G_dGCh'C Cuts at: 0 8 82 920 1109 1221 Size: 8 74 838 189 112 Fragments arranged by size: 838 189 112 74 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 590 1221 Size: 590 631 BsrBI CCG'CTC Cuts at: 0 665 1221 Size: 665 556 BsrFI r'CCGG_y Cuts at: 0 146 644 1221 Size: 146 498 577 Fragments arranged by size: 577 498 146 BstAPI GCAn_nnn'nTGC Cuts at: 0 309 591 1221 Size: 309 282 630 Fragments arranged by size: 630 309 282 BstYI r'GATC_y Cuts at: 0 245 335 587 1184 1221 Size: 245 90 252 597 37 Fragments arranged by size: 597 252 245 90 37 BtgI C'CryG_G Cuts at: 0 83 161 1221 Size: 83 78 1060 Fragments arranged by size: 1060 83 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 975 1221 Size: 975 246 DrdI GACnn_nn'nnGTC Cuts at: 0 808 1221 Size: 808 413 EaeI y'GGCC_r Cuts at: 0 71 148 1026 1221 Size: 71 77 878 195 Fragments arranged by size: 878 195 77 71 EagI C'GGCC_G Cuts at: 0 71 1221 Size: 71 1150 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 653 733 906 1221 Size: 653 80 173 315 Fragments arranged by size: 653 315 173 80 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 67 126 409 480 547 691 1221 Size: 67 59 283 71 67 144 530 Fragments arranged by size: 530 283 144 71 67 67 59 EcoNI CCTnn'n_nnAGG Cuts at: 0 391 1221 Size: 391 830 EcoO109I rG'GnC_Cy Cuts at: 0 78 79 1106 1221 Size: 78 1 1027 115 Fragments arranged by size: 1027 115 78 1 EcoRV GAT'ATC Cuts at: 0 946 1221 Size: 946 275 HaeII r_GCGC'y Cuts at: 0 141 169 277 322 1005 1221 Size: 141 28 108 45 683 216 Fragments arranged by size: 683 216 141 108 45 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 904 935 936 967 1221 Size: 904 31 1 31 254 Fragments arranged by size: 904 254 31 31 1 KasI G'GCGC_C Cuts at: 0 165 273 318 1221 Size: 165 108 45 903 Fragments arranged by size: 903 165 108 45 MslI CAynn'nnrTG Cuts at: 0 430 1045 1126 1198 1221 Size: 430 615 81 72 23 Fragments arranged by size: 615 430 81 72 23 MspA1I CmG'CkG Cuts at: 0 18 85 118 306 327 537 737 935 Size: 18 67 33 188 21 210 200 198 Cuts at: 935 1221 Size: 286 Fragments arranged by size: 286 210 200 198 188 67 33 21 18 NaeI GCC'GGC Cuts at: 0 148 646 1221 Size: 148 498 575 Fragments arranged by size: 575 498 148 NarI GG'CG_CC Cuts at: 0 166 274 319 1221 Size: 166 108 45 902 Fragments arranged by size: 902 166 108 45 NgoMIV G'CCGG_C Cuts at: 0 146 644 1221 Size: 146 498 577 Fragments arranged by size: 577 498 146 NspI r_CATG'y Cuts at: 0 1044 1146 1221 Size: 1044 102 75 Fragments arranged by size: 1044 102 75 PasI CC'CwG_GG Cuts at: 0 819 1221 Size: 819 402 PciI A'CATG_T Cuts at: 0 1040 1221 Size: 1040 181 PfoI T'CCnGG_A Cuts at: 0 338 386 1221 Size: 338 48 835 Fragments arranged by size: 835 338 48 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1027 1059 1221 Size: 1027 32 162 Fragments arranged by size: 1027 162 32 PspOMI G'GGCC_C Cuts at: 0 78 1105 1221 Size: 78 1027 116 Fragments arranged by size: 1027 116 78 PstI C_TGCA'G Cuts at: 0 332 374 587 1221 Size: 332 42 213 634 Fragments arranged by size: 634 332 213 42 PvuII CAG'CTG Cuts at: 0 306 537 737 1221 Size: 306 231 200 484 Fragments arranged by size: 484 306 231 200 SacII CC_GC'GG Cuts at: 0 86 1221 Size: 86 1135 SfcI C'TryA_G Cuts at: 0 328 370 583 1221 Size: 328 42 213 638 Fragments arranged by size: 638 328 213 42 SfoI GGC'GCC Cuts at: 0 167 275 320 1221 Size: 167 108 45 901 Fragments arranged by size: 901 167 108 45 SmaI CCC'GGG Cuts at: 0 61 1221 Size: 61 1160 SmlI C'TyrA_G Cuts at: 0 249 265 921 1221 Size: 249 16 656 300 Fragments arranged by size: 656 300 249 16 StyI C'CwwG_G Cuts at: 0 314 503 1221 Size: 314 189 718 Fragments arranged by size: 718 314 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 531 1221 Size: 531 690 TatI w'GTAC_w Cuts at: 0 456 1037 1221 Size: 456 581 184 Fragments arranged by size: 581 456 184 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 758 1221 Size: 758 463 XmaI C'CCGG_G Cuts at: 0 59 1221 Size: 59 1162 Enzymes that do cut: AccI AclI AcuI AfeI AflIII AlwNI ApaI AvaI BaeI BanI BanII BbeI BciVI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstYI BtgI BtgZI DrdI EaeI EagI EciI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KasI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PfoI PpiI PspOMI PstI PvuII SacII SfcI SfoI SmaI SmlI StyI TaqII TatI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbsI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI BmtI BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EarI EcoICRI EcoRI FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI