################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MscI MseI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tru9I Tsp509I TspRI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 479 TCCGGA T^CCGGA AfaI 487 GTAC GT^AC AlwI 630 GGATC GGATC(4/5) ApaI 703 GGGCCC GGGCC^C BanI 344 GGCACC G^G[CT][GA]CC BfaI 289 CTAG C^TAG BseAI 479 TCCGGA T^CCGGA BsmFI 13 GTCCC GGGAC(10/14) BspEI 479 TCCGGA T^CCGGA BspMI 283 ACCTGC ACCTGC(4/8) BsrBI 358 GAGCGG CCGCTC(-3/-3) BstEII 279 GGTCACC G^GT[ACGT]ACC BstPI 279 GGTCACC G^GT[ACGT]ACC BstXI 410 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 630 GGATCT [GA]^GATC[CT] DpnII 631 GATC ^GATC DrdI 4 GACATCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 410 CCATGG C^C[GA][CT]GG EaeI 119 TGGCCG [CT]^GGCC[GA] EarI 172 CTCTTC CTCTTC(1/4) EcoNI 468 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 279 GGTCACC G^GT[ACGT]ACC HinfI 129 GACTC G^A[ACGT]TC Ksp632I 172 CTCTTC CTCTTC(1/4) MaeI 289 CTAG C^TAG MaeIII 280 GTCAC ^GT[ACGT]AC MboI 631 GATC ^GATC MboII 173 TCTTC GAAGA(8/7) MflI 630 GGATCT [GA]^GATC[CT] MroI 479 TCCGGA T^CCGGA NcoI 410 CCATGG C^CATGG NdeI 536 CATATG CA^TATG NdeII 631 GATC ^GATC NspI 522 GCATGT [GA]CATG^[CT] PflMI 708 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 129 GACTC GAGTC(4/5) PpuMI 464 AGGTCCT [GA]G^G[AT]CC[CT] RsaI 487 GTAC GT^AC SacI 169 GAGCTC GAGCT^C SapI 171 GCTCTTC GCTCTTC(1/4) Sau3AI 631 GATC ^GATC SbfI 468 CCTGCAGG CCTGCA^GG ScaI 486 AGTACT AGT^ACT SmlI 634 CTTGAG C^T[CT][GA]AG Sse8387I 468 CCTGCAGG CCTGCA^GG TaqI 572 TCGA T^CGA Tsp45I 280 GTCAC ^GT[GC]AC TthHB8I 572 TCGA T^CGA Van91I 708 CCACATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 630 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 25 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 85 CAGCCCCTG BpmI 223 CTGGAG CTGGAG(16/14) 377 CTGGAG BsaI 492 GAGACC GGTCTC(1/5) 619 GAGACC BsaJI 410 CCATGG C^C[ACGT][ACGT]GG 457 CCAAGG BsaWI 241 ACCGGA [AT]^CCGG[AT] 479 TCCGGA BsrI 222 ACTGG ACTGG(1/-1) 645 CCAGT Cac8I 80 GCTGGC GC[ACGT]^[ACGT]GC 396 GCTGGC DdeI 335 CTGAG C^T[ACGT]AG 490 CTGAG DraII 464 AGGTCCT [GA]G^G[ACGT]CC[CT] 703 GGGCCCC EcoO109I 464 AGGTCCT [GA]G^G[ACGT]CC[CT] 703 GGGCCCC EcoT14I 410 CCATGG C^C[AT][AT]GG 457 CCAAGG HphI 281 TCACC GGTGA(8/7) 611 TCACC MslI 411 CATGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 429 CACTCTGGTG MspA1I 25 CAGCTG C[AC]G^C[GT]G 394 CAGCTG MwoI 27 GCTGCTGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 30 GCTGGTGCTGC PvuII 25 CAGCTG CAG^CTG 394 CAGCTG StyI 410 CCATGG C^C[AT][AT]GG 457 CCAAGG XcmI 410 CCATGGATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 535 CCATATGGAAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI AspHI AvaII BanII BbvI BcnI BmyI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cfr13I EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MnlI MspI MvaI NciI NlaIII NlaIV PstI Sau96I ScrFI SfcI TseI