(Linear) (Six-base) MAPSORT of: KIEE3083.seq Check: 3593 from: 1 to: 732 KIEE3083.orf With 182 enzymes: SgfI * March 22, 2012 16:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 292 732 Size: 292 440 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 732 Size: 159 573 AleI CACnn'nnGTG Cuts at: 0 433 732 Size: 433 299 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 451 732 Size: 451 281 AlwNI CAG_nnn'CTG Cuts at: 0 30 90 732 Size: 30 60 642 Fragments arranged by size: 642 60 30 ApaI G_GGCC'C Cuts at: 0 707 732 Size: 707 25 BanI G'GyrC_C Cuts at: 0 344 732 Size: 344 388 BanII G_rGCy'C Cuts at: 0 173 457 578 707 732 Size: 173 284 121 129 25 Fragments arranged by size: 284 173 129 121 25 Bme1580I G_kGCm'C Cuts at: 0 707 732 Size: 707 25 BmrI ACTGGGnnnn_n' Cuts at: 0 639 732 Size: 639 93 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 523 732 Size: 523 209 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 244 398 732 Size: 244 154 334 Fragments arranged by size: 334 244 154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 655 732 Size: 655 77 BsaI GGTCTCn'nnnn_ Cuts at: 0 486 613 732 Size: 486 127 119 Fragments arranged by size: 486 127 119 BsaWI w'CCGG_w Cuts at: 0 241 479 732 Size: 241 238 253 Fragments arranged by size: 253 241 238 BseYI C'CCAG_C Cuts at: 0 72 228 545 732 Size: 72 156 317 187 Fragments arranged by size: 317 187 156 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 411 469 732 Size: 40 371 58 263 Fragments arranged by size: 371 263 58 40 BsiHKAI G_wGCw'C Cuts at: 0 173 445 509 732 Size: 173 272 64 223 Fragments arranged by size: 272 223 173 64 Bsp1286I G_dGCh'C Cuts at: 0 173 445 457 509 578 707 732 Size: 173 272 12 52 69 129 25 Fragments arranged by size: 272 173 129 69 52 25 12 BspEI T'CCGG_A Cuts at: 0 479 732 Size: 479 253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 292 732 Size: 292 440 BsrBI CCG'CTC Cuts at: 0 360 732 Size: 360 372 BstEII G'GTnAC_C Cuts at: 0 279 732 Size: 279 453 BstXI CCAn_nnnn'nTGG Cuts at: 0 417 732 Size: 417 315 BstYI r'GATC_y Cuts at: 0 630 732 Size: 630 102 BtgI C'CryG_G Cuts at: 0 410 732 Size: 410 322 DrdI GACnn_nn'nnGTC Cuts at: 0 10 732 Size: 10 722 EaeI y'GGCC_r Cuts at: 0 119 732 Size: 119 613 EarI CTCTTCn'nnn_ Cuts at: 0 178 732 Size: 178 554 EcoICRI GAG'CTC Cuts at: 0 171 732 Size: 171 561 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 159 244 398 732 Size: 159 85 154 334 Fragments arranged by size: 334 159 154 85 EcoNI CCTnn'n_nnAGG Cuts at: 0 472 732 Size: 472 260 EcoO109I rG'GnC_Cy Cuts at: 0 465 704 732 Size: 465 239 28 Fragments arranged by size: 465 239 28 MslI CAynn'nnrTG Cuts at: 0 415 433 732 Size: 415 18 299 Fragments arranged by size: 415 299 18 MspA1I CmG'CkG Cuts at: 0 27 396 732 Size: 27 369 336 Fragments arranged by size: 369 336 27 NcoI C'CATG_G Cuts at: 0 410 732 Size: 410 322 NdeI CA'TA_TG Cuts at: 0 537 732 Size: 537 195 NspI r_CATG'y Cuts at: 0 526 732 Size: 526 206 PflMI CCAn_nnn'nTGG Cuts at: 0 714 732 Size: 714 18 PfoI T'CCnGG_A Cuts at: 0 205 375 732 Size: 205 170 357 Fragments arranged by size: 357 205 170 PpuMI rG'GwC_Cy Cuts at: 0 465 732 Size: 465 267 PspOMI G'GGCC_C Cuts at: 0 703 732 Size: 703 29 PstI C_TGCA'G Cuts at: 0 26 277 473 732 Size: 26 251 196 259 Fragments arranged by size: 259 251 196 26 PvuII CAG'CTG Cuts at: 0 27 396 732 Size: 27 369 336 Fragments arranged by size: 369 336 27 SacI G_AGCT'C Cuts at: 0 173 732 Size: 173 559 SapI GCTCTTCn'nnn_ Cuts at: 0 178 732 Size: 178 554 SbfI CC_TGCA'GG Cuts at: 0 473 732 Size: 473 259 ScaI AGT'ACT Cuts at: 0 488 732 Size: 488 244 SfcI C'TryA_G Cuts at: 0 22 273 469 732 Size: 22 251 196 263 Fragments arranged by size: 263 251 196 22 SmlI C'TyrA_G Cuts at: 0 634 732 Size: 634 98 StyI C'CwwG_G Cuts at: 0 410 457 732 Size: 410 47 275 Fragments arranged by size: 410 275 47 TatI w'GTAC_w Cuts at: 0 486 732 Size: 486 246 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 417 542 732 Size: 417 125 190 Fragments arranged by size: 417 190 125 Enzymes that do cut: AarI AcuI AleI AlfI AlwNI ApaI BanI BanII Bme1580I BmrI BplI BpmI BpuEI BsaI BsaWI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BstEII BstXI BstYI BtgI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I MslI MspA1I NcoI NdeI NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI SbfI ScaI SfcI SmlI StyI TatI XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI