Restriction map for
pFN21AB8944
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccI AccIII AclI
AfaI AflII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbeI BbrPI BciVI BclI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsgI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI DraI DrdI Eam1105I EarI
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsaI SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI TspRI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 41 CGCG CG^CG
AcyI 567 GGCGTC G[GA]^CG[CT]C
AflIII 761 ACGTGT A^C[GA][CT]GT
Aor51HI 704 AGCGCT AGC^GCT
BalI 650 TGGCCA TGG^CCA
BbsI 139 GTCTTC GAAGAC(2/6)
BcgI 73 CGAGGGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 173 AGATCT A^GATCT
BpuAI 139 GTCTTC GAAGAC(2/6)
BsaHI 567 GGCGTC G[GA]^CG[CT]C
BsaWI 631 TCCGGT [AT]^CCGG[AT]
BspHI 341 TCATGA T^CATGA
BstAPI 23 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 717 TTCGAA TT^CGAA
BstUI 41 CGCG CG^CG
DraII 158 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 643 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 284 CGGCCG C^GGCCG
EclXI 284 CGGCCG C^GGCCG
Eco47III 704 AGCGCT AGC^GCT
Eco52I 284 CGGCCG C^GGCCG
EcoNI 150 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 158 AGGCCCC [GA]G^G[ACGT]CC[CT]
Hin1I 567 GGCGTC G[GA]^CG[CT]C
HphI 329 TCACC GGTGA(8/7)
MaeII 761 ACGT A^CGT
MaeIII 328 GTCAC ^GT[ACGT]AC
MluNI 650 TGGCCA TGG^CCA
MscI 650 TGGCCA TGG^CCA
MseI 5 TTAA T^TAA
MspA1I 790 CAGCTG C[AC]G^C[GT]G
MvnI 41 CGCG CG^CG
NspI 119 ACATGC [GA]CATG^[CT]
NspV 717 TTCGAA TT^CGAA
PflMI 642 CCACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 563 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 790 CAGCTG CAG^CTG
RcaI 341 TCATGA T^CATGA
SfaNI 801 GATGC GCATC(5/9)
SfuI 717 TTCGAA TT^CGAA
SmaI 572 CCCGGG CCC^GGG
SmlI 443 CTCAAG C^T[CT][GA]AG
Tru9I 5 TTAA T^TAA
Tsp45I 328 GTCAC ^GT[GC]AC
Van91I 642 CCACTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 235 CCATCCGGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 572 CCCGGG C^CCGGG
XmaI 572 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 721 AAATTC [GA]^AATT[CT]
830 AAATTT
AlwI 336 GATCC GGATC(4/5)
729 GATCC
ApoI 721 AAATTC [GA]^AATT[CT]
830 AAATTT
AvaII 207 GGTCC G^G[AT]CC
816 GGACC
BanII 502 GAGCCC G[GA]GC[CT]^C
538 GAGCCC
BbvCI 577 GCTGAGG CCTCAGC(-5/-2)
601 GCTGAGG
BpmI 229 CTGGAG CTGGAG(16/14)
739 CTGGAG
BsaI 875 GAGACC GGTCTC(1/5)
216 GGTCTC
BsiEI 284 CGGCCG CG[GA][CT]^CG
453 CGACCG
BsmAI 875 GAGAC GTCTC(1/5)
217 GTCTC
CpoI 206 CGGTCCG CG^G[AT]CCG
815 CGGACCG
DsaI 134 CCACGG C^C[GA][CT]GG
647 CCGTGG
EcoT14I 530 CCAAGG C^C[AT][AT]GG
653 CCAAGG
HaeII 382 AGCGCC [GA]GCGC^[CT]
704 AGCGCT
HgaI 434 GCGTC GACGC(5/10)
568 GCGTC
HincII 13 GTCAAC GT[CT]^[GA]AC
421 GTCAAC
HindII 13 GTCAAC GT[CT]^[GA]AC
421 GTCAAC
NaeI 403 GCCGGC GCC^GGC
660 GCCGGC
NgoMIV 403 GCCGGC G^CCGGC
660 GCCGGC
NlaIII 120 CATG CATG^
342 CATG
PstI 787 CTGCAG CTGCA^G
883 CTGCAG
RsrII 206 CGGTCCG CG^G[AT]CCG
815 CGGACCG
SfcI 787 CTGCAG C^T[GA][CT]AG
883 CTGCAG
StyI 530 CCAAGG C^C[AT][AT]GG
653 CCAAGG
Tsp509I 722 AATT ^AATT
831 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AvaI BanI BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
TaqI TseI TthHB8I XhoII