Restriction map for
pFN21AB8944
(Linear) (Six-base) MAPSORT of: KIBB8944.seq Check: 3149 from: 1 to: 912
KIBB8944.orf
With 182 enzymes: SgfI *
March 22, 2012 15:45 ..
AfeI AGC'GCT
Cuts at: 0 706 912
Size: 706 206
AflIII A'CryG_T
Cuts at: 0 761 912
Size: 761 151
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 250 282 912
Size: 250 32 630
Fragments arranged by size:
630 250 32
ApoI r'AATT_y
Cuts at: 0 721 830 912
Size: 721 109 82
Fragments arranged by size:
721 109 82
AvaI C'yCGr_G
Cuts at: 0 124 541 558 572 894 912
Size: 124 417 17 14 322 18
Fragments arranged by size:
417 322 124 18 17 14
BanI G'GyrC_C
Cuts at: 0 248 546 586 912
Size: 248 298 40 326
Fragments arranged by size:
326 298 248 40
BanII G_rGCy'C
Cuts at: 0 506 542 912
Size: 506 36 370
Fragments arranged by size:
506 370 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 132 912
Size: 132 780
BbvCI CC'TCA_GC
Cuts at: 0 578 602 912
Size: 578 24 310
Fragments arranged by size:
578 310 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 62 96 912
Size: 62 34 816
Fragments arranged by size:
816 62 34
BglII A'GATC_T
Cuts at: 0 173 912
Size: 173 739
Bme1580I G_kGCm'C
Cuts at: 0 203 912
Size: 203 709
BmrI ACTGGGnnnn_n'
Cuts at: 0 98 912
Size: 98 814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 760 912
Size: 250 510 152
Fragments arranged by size:
510 250 152
Bpu10I CC'TnA_GC
Cuts at: 0 578 602 912
Size: 578 24 310
Fragments arranged by size:
578 310 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 912
Size: 428 484
BsaI GGTCTCn'nnnn_
Cuts at: 0 222 869 912
Size: 222 647 43
Fragments arranged by size:
647 222 43
BsaHI Gr'CG_yC
Cuts at: 0 568 912
Size: 568 344
BsaWI w'CCGG_w
Cuts at: 0 631 912
Size: 631 281
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 250 280 340 370 733 763 912
Size: 250 30 60 30 363 30 149
Fragments arranged by size:
363 250 149 60 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 258 405 619 622 912
Size: 258 147 214 3 290
Fragments arranged by size:
290 258 214 147 3
BseYI C'CCAG_C
Cuts at: 0 379 622 912
Size: 379 243 290
Fragments arranged by size:
379 290 243
BsiEI CG_ry'CG
Cuts at: 0 287 456 912
Size: 287 169 456
Fragments arranged by size:
456 287 169
Bsp1286I G_dGCh'C
Cuts at: 0 203 506 542 912
Size: 203 303 36 370
Fragments arranged by size:
370 303 203 36
BspHI T'CATG_A
Cuts at: 0 341 912
Size: 341 571
BsrFI r'CCGG_y
Cuts at: 0 403 660 878 912
Size: 403 257 218 34
Fragments arranged by size:
403 257 218 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 29 912
Size: 29 883
BstBI TT'CG_AA
Cuts at: 0 718 912
Size: 718 194
BstYI r'GATC_y
Cuts at: 0 173 335 728 912
Size: 173 162 393 184
Fragments arranged by size:
393 184 173 162
BtgI C'CryG_G
Cuts at: 0 134 647 912
Size: 134 513 265
Fragments arranged by size:
513 265 134
DraIII CAC_nnn'GTG
Cuts at: 0 648 912
Size: 648 264
EaeI y'GGCC_r
Cuts at: 0 284 366 650 662 912
Size: 284 82 284 12 250
Fragments arranged by size:
284 284 250 82 12
EagI C'GGCC_G
Cuts at: 0 284 912
Size: 284 628
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 610 912
Size: 610 302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 760 912
Size: 250 510 152
Fragments arranged by size:
510 250 152
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 912
Size: 154 758
EcoO109I rG'GnC_Cy
Cuts at: 0 159 912
Size: 159 753
HaeII r_GCGC'y
Cuts at: 0 386 708 912
Size: 386 322 204
Fragments arranged by size:
386 322 204
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 165 197 912
Size: 165 32 715
Fragments arranged by size:
715 165 32
HincII GTy'rAC
Cuts at: 0 15 423 912
Size: 15 408 489
Fragments arranged by size:
489 408 15
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 912
Size: 113 799
MscI TGG'CCA
Cuts at: 0 652 912
Size: 652 260
MspA1I CmG'CkG
Cuts at: 0 792 912
Size: 792 120
NaeI GCC'GGC
Cuts at: 0 405 662 912
Size: 405 257 250
Fragments arranged by size:
405 257 250
NgoMIV G'CCGG_C
Cuts at: 0 403 660 912
Size: 403 257 252
Fragments arranged by size:
403 257 252
NspI r_CATG'y
Cuts at: 0 123 912
Size: 123 789
PflMI CCAn_nnn'nTGG
Cuts at: 0 648 912
Size: 648 264
PfoI T'CCnGG_A
Cuts at: 0 209 731 796 912
Size: 209 522 65 116
Fragments arranged by size:
522 209 116 65
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 912
Size: 250 32 630
Fragments arranged by size:
630 250 32
PshAI GACnn'nnGTC
Cuts at: 0 567 912
Size: 567 345
PstI C_TGCA'G
Cuts at: 0 791 887 912
Size: 791 96 25
Fragments arranged by size:
791 96 25
PvuII CAG'CTG
Cuts at: 0 792 912
Size: 792 120
RsrII CG'GwC_CG
Cuts at: 0 207 816 912
Size: 207 609 96
Fragments arranged by size:
609 207 96
SfcI C'TryA_G
Cuts at: 0 787 883 912
Size: 787 96 29
Fragments arranged by size:
787 96 29
SmaI CCC'GGG
Cuts at: 0 574 912
Size: 574 338
SmlI C'TyrA_G
Cuts at: 0 443 912
Size: 443 469
StyI C'CwwG_G
Cuts at: 0 530 653 912
Size: 530 123 259
Fragments arranged by size:
530 259 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 833 912
Size: 833 79
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 242 912
Size: 242 670
XmaI C'CCGG_G
Cuts at: 0 572 912
Size: 572 340
Enzymes that do cut:
AfeI AflIII AloI ApoI AvaI BanI BanII BbsI
BbvCI BcgI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI Bsp1286I
BspHI BsrFI BstAPI BstBI BstYI BtgI DraIII EaeI
EagI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
MmeI MscI MspA1I NaeI NgoMIV NspI PflMI PfoI
PpiI PshAI PstI PvuII RsrII SfcI SmaI SmlI
StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI BplI BsaAI BsaBI BsgI
BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EarI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MslI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpuMI PsiI PspOMI PspXI PsrI PvuI SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI